Scribd
Upload
737 views

Lab Stain Chart

Micro-Lab Stain Organization Chart Stain Type Simple Counter Stain Mordant Decolonize Result B. Cereus Beige Colony S. Aureus White Colony morphology cell 2 cell arrangement only microbes 2 diseases caused by myobacterium TB and leprosy Stained waxy cell wall is not decolonize by Acid Alcohol (-)

Uploaded by

Frankiesgirl6yr
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as ODT, PDF, TXT or read online on Scribd
737 views

Lab Stain Chart

Micro-Lab Stain Organization Chart Stain Type Simple Counter Stain Mordant Decolonize Result B. Cereus Beige Colony S. Aureus White Colony morphology cell 2 cell arrangement only microbes 2 diseases caused by myobacterium TB and leprosy Stained waxy cell wall is not decolonize by Acid Alcohol (-)

Uploaded by

Frankiesgirl6yr
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as ODT, PDF, TXT or read online on Scribd
You are on page 1/ 2

Micro-Lab

Stain Organization Chart Subject of Study 3

Simple
Stain Type Counter Mordant Decolonize Result Comment
Stain
B. cereus Found on quadrant
Beige strand TSA agar.
Methylene Colony White Colony
Simple Stain NA NA
Blue S. aureus Beige Colony
White Morphology
Colony Cell 2 Cell Arrange
Agar slant Flame loop & add
culture of drop of H2O
B. cereus Flame loop & add a
Methylene
Smear Prep NA NA Nutrient small amount of cult.
Blue
broth culture Heat fix attaches
of S. aureus bacterial smear to
slide.
Crystal Iodine acts as a
Violet mordant
Gram +
(Primary) Decolonize carefully
Purple
95% Ethyl Basis of classifying
Gram Stain Gram Iodine
Safranin Alcohol function of
Gram -
(Counter) morphology
Pink/Red
Cell 2 Cell
arrangement
Carbon Acid Myobacteriu Only microbes
fusion Alcohol (Red/rods) 2 diseases caused by
(Primary) “retains myobacterium
Fast-Acid
NA primary S. aureus TB & leprosy
Stain
Methylene stain” (Blue/cocci) Stained waxy cell
Blue wall is not decolonize
(Counter) by acid alcohol
(-) stain procedure
Gray – Black Will not absorb die,
Methylene background but back ground is
Blue Clear oval colored
Capsule (-) NA NA spaces each DO Not
Nigrosin containing a • heat fix
(acidic) small blue • rinse
bacillus
• blot
Micro-Lab

Vegetative Has thick spore


Cell coat
Malachite (pink spores) Does not absorb die
green (small green Heat allows die to
(Primary) spores) penetrate.
Spore Stain NA Water
Metallic
Safranin inactive cells
(Counter) types are spores

Important Terms Related to Staining

Dyes – Chemicals used to add color to the cell.


Acidic Stain – are anionic, negative charge (Sodium, Potassium, Calcium)
Basic Stain – are cationic, positive charge (Chloride, Sulfate salts)
Staining – The process of adding color to a bacterial cell
Simple Stain- uses only 1 dye.
Allows observation of
morphology
cell to cell arrangement
Differential Stain – uses 2 or more dyes
Allows observation of
morphology
cell to cell arrangement
property/ structure
Crystal violet – Iodine crystal formation in cell
Gram + Alcohol dehydrates cell wall
CV-I, crystal formation is unable to leave cell
thus leaving purple dye
Gram – Alcohol dissolves outer membrane, leaving wholes
in wall, thus allowing CV-I to wash out
Heat Fix – fixes bacterial smear to slide, preventing smear from washing away during staining
Chromogen – Colored compound, not stain

You might also like