Filamentous Fungi and Yeast
Filamentous Fungi and Yeast
Filamentous Fungi and Yeast
Eukaryotehttp://www.windows.ucar.edu/earth/Life/images/celltypes.gif
Dimorphism
Fungus that can
present 2 forms
depending of
conditions
Yeast
Filamentous fungi
Could be
opportunistic
pathogens
http://gsbs.utmb.edu/microbook/images/fig75_3.JPG
Yeast
Unicellular
Non filamentous
Spherical
Membrane bound nucleus
are eukaryote
Facultative anaerobes
http://www.theartisan.net/yeast_cell_final_resample.jpg
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/Y/Yeast.jpg
Filamentous fungi
Multicellular
organism
Long branched
filament called
hyphae
Hyphae aggregate
to form a mass
mycelium
Aerobes
http://www.anselm.edu/homepage/jpitocch/genbios/31-01-FungalMycelia-L.jpg
Sabouraud agar
Selective media
Contain sugars and
peptone
Low pH (5) which is
inhibitory for most other
microorganisms
Invented by a French
Doctor (Dr. Sabouraud)
specialist in scalp
disease
http://www.bium.univ-paris5.fr/sfhd/img/gd/sabou.jpg
Reproduction
Sexual and asexual
Asexual
Budding:The parent cell can divide into two
equal or unequal daughter cells
Spore prodution:
Sporangiospores
Conidiospores
http://www.anselm.edu/homepage/jpitocch/genbios/31-01-FungalMycelia-L.jpg
Reproduction
Budding: Yeast
http://www.mansfield.ohio-state.edu/~sabedon/018yeast.gif
Reproduction Conidiospores: are not
enclosed within a sac
Sporangiospores: enclosed
in a sac like head called a
sporangium
Mold growth
http://ipm.ncsu.edu/current_ipm/colony.jpg http://www.inspect-ny.com/sickhouse/1263s.jpg
http://byebyemold.com/mold_images/images/penicillium/penicillium_c.jpg http://www.mould.ph/images/curvul3.jpg
Aspergillus niger
http://res2.agr.ca/winnipeg/storage/images/lo-res/mould/aspe06-l.jpg
http://www.ttuhsc.edu/SOM/Microbiology/mainweb/aiaq/Pictures/Aspergillus%20niger.jpg
http://wellino.de/aspergillus/images/aspergillus-niger-5.jpg
Penicillium frequentans
http://www.anselm.edu/homepage/jpitocch/genbios/31-14b-Penicillium.jpg
http://www.bioweb.uncc.edu/1110Lab/notes/notes1/labpics/Penicillium%20notatum.JPG
Objective 1: Culturing yeast
A. Fermentation tubes
Inoculate each yeast into a glucose and a
sucrose fermentation tube
2 tubes per yeast, 8 total
Incubate at 37oC
Objective 1A: Culturing yeast
Next lab (Wednesday)
Glucose Sucrose
2. Saccharomyces
cerevisiae
3. Candida albicans
4. Rhodoturula rubra
Objective 1B: Culturing yeast
Streak yeast
Divide 2 sabouraud plate in a half
We have 4 different yeast
Inoculate one per half (As you did for bacteria)
Macroscopic:
Colony appearance
Hyphae color
Spore color
Underside color
Objective 2.B Environmental
sample Molds
http://www.botany.utoronto.ca/ResearchLabs/MallochLab/Malloch/Moulds/Illustrations/Slide_culture.jpg
Slide culture
http://www.botany.utoronto.ca/ResearchLabs/MallochLab/Malloch/Moulds/Illustrations/Slide_culture.jpg
Slide culture
http://www.botany.utoronto.ca/ResearchLabs/MallochLab/Malloch/Moulds/Illustrations/Slide_culture.jpg
Synthesis
Culture 4 yeast : 2 Petri plates
Culture 4 glucose fermentation tubes
Culture 4 sucrose fermentation tubes
Culture 4 molds
Slide culture