Lugol's Recipe

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Here is the recipe:

Put 20g of Potassium iodide (KIO3)


and 10g resumblimed Iodine (I2)
into 200ml demineralized/distilled water
with 20ml concentrated acetic acid (CH3COOH)
and mix. It dissolves quite readily.

A recipe for Lugol's Solution:


10gm Potassium Iodide in 20mls distilled water,
5gm Iodine twice sublimed in 50mls distilled water
Stir to dissolve then add 5gms Sodium Acetate and continue to stir
until dissolved. Combine and store in dark reagent bottles. Recipe
may be doubled or tripled. Use approximately 1ml Lugol's /100ml's
lake water and dinoflagellates preserve fine. Samples after killing should be
preserved in 2% final concentration formaldehyde.

Here is the recipe:


20 gram potassium iodide
10 gram iodine
200 mL distilled water
20 mL glacial acetic acid
Replacing glacial acetic acid with sodium acetate makes the solution
neutral or slightly basic; this allows preservation of Coccolithophores.

Lugol's solution is commonly used for short-term (e.g. a few months, but
possibly a year or more) storage of cyanobacteria. Dissolve one gram of iodine
crystals and two grams of potassium iodide in 300 ml of water. Use three drops of
this solution in a 100 ml sample (it should look like very weak tea).

Hazards: Harmful if swallowed. May cause irritation. Avoid breathing vapors, or dusts. Use with
adequate ventilation. Avoid contact with eyes, skin, and clothes. Wash thoroughly after handling.
Keep container closed. Conditions aggravated/target organs: Persons with pre-existing skin, eye,
thyroid, and respiratory disorders, as well as persons with iodine allergies, will be more susceptible.
May cause skin and eye irritation. Conditions aggravated: Thyroid disorders,

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