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DNA Extraction Virtual Lab

This document provides instructions for a virtual DNA extraction lab. It guides students through extracting DNA from cheek cells using a buccal swab. The four main steps are: 1) collecting cheek cells, 2) bursting open the cells to release DNA, 3) separating DNA from other cell components, and 4) isolating concentrated DNA. A variety of solutions are used, including lysis solution to break open cells, salt solution to precipitate proteins, and isopropyl alcohol to make DNA visible. Centrifugation is used to separate DNA from other cell debris.

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1K views2 pages

DNA Extraction Virtual Lab

This document provides instructions for a virtual DNA extraction lab. It guides students through extracting DNA from cheek cells using a buccal swab. The four main steps are: 1) collecting cheek cells, 2) bursting open the cells to release DNA, 3) separating DNA from other cell components, and 4) isolating concentrated DNA. A variety of solutions are used, including lysis solution to break open cells, salt solution to precipitate proteins, and isopropyl alcohol to make DNA visible. Centrifugation is used to separate DNA from other cell debris.

Uploaded by

api-320549212
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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DNA Extraction Virtual Lab

http://learn.genetics.utah.edu/content/labs/extraction/

Directions: Using the link above, go through the virtual lab and answer the following questions as you proceed
through the lab.
1. What reasons would we have for extracting human DNA (3 things)?
1) Test newborns for genetic diseases 2) Analyze forensic evidence 3) Study a gene involved with cancer
2. Where do we find DNA in the cell (hint: watch the animation)?
DNA is found in chromosome s located in the cells nucleus.
3. Why is the inside of the cheek a good place to get a cell sample?
It losses thousands of cells everyday, so it wont cause any damage to the person.
4. List the four steps that you will follow to collect and purify DNA?
1) Collect Cheek Cells 2) Burst Cells Open to Release DNA 3) Separate DNA from proteins and debris
4) Isolate Concentrated DNA
5. List the equipment that will be used.
Warm Water Bath, Centrifuge, Buccal Swab, Sample Tubes, Micropippetors, Lysis Solution, Concentrated Salt
Solution, Resuspension Buffer, ethanol, isopryol alcohol
6. What is the procedure for collecting cheek cells?
Swabbing the mouth with a Buccal Swab and placing it into an Eppendorf.
7. What does lysis mean? What is the lysis solution used for?
Lysis a Greek word meaning to separate. The lysis solution is used to separate the cheek cells from the Buccal
swab.
8. List the two ingredients found in a lysis solution and what their purposes are.
a. Detergent- Disrupts cell membrane and nuclear envelope, with the cell bursting and releasing DNA.
b. Proteinase K Cuts apart histones to free DNA.

9. What is the purpose of adding salt solution?


The salt from the solution causes proteins and other cellular debris to clump together.
10. What is the purpose of adding another test tube full of water in the centrifuge?
The centrifuge needs to be balanced.
11. Whats the centrifuge do?

The centrifuge spins around at high speeds, and it pushes the unwanted cell debris down to the bottom of the
tube.
12. Sketch the distribution of the cell parts, proteins, and DNA in the test tube after being removed from the
centrifuge.

13. What is done with the DNA after being spun in the centrifuge?
The DNA is carefully relocated to a new tube without the cell debris.
14. What is the procedure for mixing isopropyl alcohol with the DNA?
Using a minipippetor, you take the isopropyl alcohol and put it in the DNA tube. Then you shake the tube
lightly.
15. How does isopropyl alcohol change the state of the DNA?
It makes the DNA come out of the solution, do now it is visible to the naked eye.
16. What happens after the DNA is again spun in the centrifuge?
When the DNA was spun in the centrifuge, it got squeezed to the bottom. The liquid is removed and you let
the DNA dry.

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