Biology Study Material Clxii2011-2012

BIOLOGY
SUPPORT MATERIAL
2011 - 2012
CLASS XII
Kendriya Vidyalaya Sangathan

18 Institutional Area
Shaheed Jeet Singh Marg
New Delhi - 1110016
MEMBERS OF THE CONTRIBUTING TEAM
1.Mr. S.C.SAHA
PGT BIOLOGY -K.V. No.1 BINAGURI
2. Mrs. SOMA BANERJEE
PGT BIOLOGY-K.V. BURDWAN
3.Mrs. RUBY .C. DESHMUKH
PGT BIOLOGY-K.V. SALUA
4. Dr. SABYASACHI MAJUMDAR
PGT BIOLOGY-K.V. MALDA
5.Mr GAUTAM BISWAS
PGT BIOLOGY-K.V. BARRACKPORE (ARMY)
6.Mr.P.K.MAHAPATRA
PGT BIOLOGY-K.V. BAMANGACHI
7.Mrs. LIPIKA DEY
PGT BIOLOGY-K.V. No.1 KANCHRAPARA
8.Mrs. G. VIJAY LAKSHMI
PGT BIOLOGY-K.V. BARRACKPORE (AFS)
ACKNOWLEDGEMENT
CHIEF PATRON
SRI AVINASH DIKSHIT, IDAS
COMMISSIONER
KENDRIYA VIDYALAYA SANGATHAN
NEW DELHI
PATRONS
SHAIK MOHAMMAD SALEEM
ASSISTANT COMMISSIONER
KOLKATA REGION
Ms. CHANDANA MANDAL

EDUCATION OFFICER
KOLKATA REGION
CONVENOR
DR. (Mrs.) TANUJA SAMAL
PRINCIPAL
KENDRIYA VIDYALAYA
COMMAND HOSPITAL
CONTENTS
Sl No
Chapter
Pages
1
2
3
4
5
6
7
8
9
5.1, 5 8
8.1, 9 12
12.1 12.6, 13 25
25.1 25.3, 26 37
37.1, 38 46
46.1, 47 56
56.1, 57 63
63.1 63.7, 64 65
65.1, 66 68
10
11
12
13
14
Reproduction in animal
Sexual reproduction in flowering plants
Human reproduction
Reproductive health
Principles of inheritance and variation
Molecular basis of inheritance
Evolution
Human health and diseases
Strategies for enhancement in food
production
Microbes in human welfare
Biotechnology:principles and processes
Biotechnology and its application
Organisms and populations
Ecosystem
15
16
Biodiversity and conservation

Environmental issues
17
18
19
20
21
Wordlist Bingo
CBSE Question & Marking Scheme
List of important diagram
High order thinking (hots)Assignments
List of abbreviations and their expansion
68.1, 69 74
75 98
98.1, 99 102
102.1, 103 -108
108.1 108.8, 109
110
110.1, 111 113
113.1 113.2, 114
120
121 125
126 132
133 134
135 145
146 147
Chapter 1: - REPRODUCTION IN ORGANISMS
REPRODUCTION IN ORGANISMS
Life Span
Period from birth till natural death.
Every organism live only for a certain period of time.

Reproduction Producing young-ones of their kind, generation after generation.
Types of reproduction:
Asexual reproduction :single parent capable of producing offspring.
Sexual reproduction : two parents are invovled in producing offspring.
Modes of asexual reproduction
Binary fission: parent body divides into two halves, genetically identical to parent.
Amoeba: It is simple or irregular. Paramoecium: Transverse binary fission.
Multiple fission: parent body divides into many daughter organisms : Plasmodium.
Budding: daughter organisms grow from small buds arising in parent body.
Exogenous budding: out side the body eg. Hydra, Yeast.
Budding in Yeast Endogenous budding : inside the body eg. Gemmule in sponge.
Conidia: non-motile, exogenous spores in chains eg. Fungi.
Zoospores: microscopic motile structures eg. Algae. In plants : term vegetative reproduction
frequently used instead of asexual reproduction, units of vegetative propagation called vegetative
propagules.
All organisms show remarkable similarity. Vast difference in their reproductive structure. Similar
pattern or phases in their life cycles.
Juvenile phase: The phase of growth before reproductive maturity.
Reproductive phase: Reproductive maturity.
Senescent phase: Phase between reproductive maturity and death.
The main events of sexual cycle are:
i. Prefertilisation events:
a.Gametogenesis :
The process of formation of male and female gametes by meiosis (cell-division).
Homogamete(Isogamete): - gametes similar eg. Algae
Heterogamete(an-isogamete): - morphologically dissimilar gamete ,male gamete (antherozoid or
sperm) ,female gamete (egg or ovum) eg. Human.
Sexuality in organisms : In plants Bisexual term is used for Homothallic and Monoecious plants
Both male and female reproductive structures in same plant eg. Higher plants, cucurbits and coconut.
Unisexual term used for Heterothallic and Dioecious plants
Male and female reproductive-structure on different plants.
Flowering plants male flowerstaminate flower and female flowerpistillate flower eg. papaya and
date-palm.
Animals Bisexual term is used for Hermaphrodite animals-eg. Earth-worm, Tape-worm, Leech,
Sponge.
Unisexual animals have male & female sexes in separate individuals-e.g. insects, frogs, human
beings
Cell division during gamete formation:
Haploid-parent (n) produces haploid gametes (n) by mitotic division, eg. Monera,fungi, algae and
bryophytes.
Diploid parent (2n) produces haploid gametes(n) by meiosis division (possess only one set of
chromosomes)and such specialized parent cell is called meiocyte or gamete mother cell.
ExampleName of organism
Meiocyte(2n)
gamete (n)
Human
46
23
Housefly
12
6
Ophioglossum (fern)
1260
630
Potato
48
24
b) Gamete transfer:- to facilitate fusion.
Male gametes mostly motile and female non-motile, exception few fungi and in algae both gametes
are motile in some cases
Water medium for gamete transfer- in lower plants. Large number of male gametes produced to
compensate loss
Higher plants, pollen-grains are transferred by pollination.
Fertilization: Fusion of male and female gametes diploid zygote.
Parthenogenesis.-development into new organism without fertilisation eg. Rotifers, honey-bees, some
lizard, bird(turkey).
Fertilization
Two types- external and internal .
External fertilisation- outside the body of organism in external- medium (water) eg. majority of algae,
fishes, amphibians.
Advantage- show great synchrony between the sexes
1. Release of large number of gametes into surrounding medium
2. Large number of offsprings produced.
Disadvantage- offspring vulnerable to predators, natural disasters.
Internal fertilisation- fusion occurs inside female body eg. majority of plants and animals. Egg nonmotile and formed inside female body. Male gamete motile, produced in large numbers to reach egg
and fuse with it. In seed plants, non- motile male gamete carried to female gamete by pollen-tube.
Post -fertilisation events- formation of zygote.
a. Zygote. One celled , diploid, vital link between two generations.
External fertilization zygote formed in external medium water eg. Frog,

Internal fertilization zygote formed inside the body eg. Human beings. Development of zygote
depends on type of life cycle and environment. Some develop thick wall ( prevent damage and
desiccation) & undergo period of rest eg. Algae, fungi.
Haplontic life cycle- zygote (2n) divides by meiosis to form haploid (n) spores.
Diplontic life-cycle- zygote (2n) divides mitotically, develops into embryo (2n).
Oviparous animals lay eggs out-side the female body. Eggs can be fertilized/ unfertilized. Fertilized
eggs covered which hard calcareous shell, laid in safe place in the environment. Unfertilised eggs laid
in water. Example- fishes, frogs, reptiles, birds
Viviparous animals bear and rear the embryo inside female body, give birth to young-ones.
Advantage- proper embryonic care, protection, survival chances of young-ones greater. Examplecows, whales, human beings
Embryogenesis: development of embryo from zygote by cell division (mitosis) and cell differentiation.
Cell- division increases the number of cells in the developing embryo
Cell differentiation - groups of cells undergo certain modifications for the formation of different kinds
of tissues and organs.
In flowering plants- zygote formed inside ovule
Changes occur in flowering plants:
Sepal
Petal
Stamen
Zygote
Primary endosperm nucleus
Synergid
Antipodals
Ovary
Ovule
Ovary wall
Integument
Fall off
Fall off
Fall off
Embryo
Endosperm (3 N)
Disintegrate
Disintegrate
Fruit
Seed
Pericarp (epicarp + mesocarp + endocarp)
Seed coat (testa + tegmen)
Parthenogenesis: Female gamete develops into new organism.

Seedless fruits formed by parthenogenesis
Clone: A group of individuals of the same species that are morphologically and genetically similar to
each other & their parents
Question & Answer

Very short answer type(1 mark)
1- What is meiocyte?
Ans: Specialized cells in diploid organism, i.e., gamete mother cell which undergo meiosis.
2- Name the kind of reproduction in bees by which drones are produced?
Ans: Parthenogenesis.
3- What is special in flowering bamboo?
Ans: Bamboo species flower only once in their life-times generally after 50-100 years.
4- What is meant by homothallic?
Ans: The term homothallic refers to bisexual or hermaphrodite condition.
5- Why are the date palms referred to as dioecious ?
Ans: In date-palms, the male and female flowers are present in different plants.
6- If the meiocyte of an onion plant contains 32 chromosomes, work out the number of
chromosome in the endosperm and embryo?
Ans: Hint: endosperm is triploid.
7- Name two acellular organisms which reproduces sexually.
Ans: Paramoecium, Plasmodium
8- Give the scientific terms for the following
Ans: a. Morphologically and genetically similar individual derived through asexual reproduction. AnsClone
b. Cyclical changes shown by seasonal breeders. Ans- Oetrous cycle
Short answer type (2 marks)
9- Name the structure which gets transformed into seeds at maturity.
Ans: ovule
10- Name any one animal in which self-fertilization occurs.
Ans: Taenia (tapeworm)
CHAPTER 2 SEXUAL REPRODUCTION INFLOWERING PLANTS

FLOWERS
Site of sex
ual Reproduction.
Male and female reproductive organs are borne on flowers.
PARTS OF A FLOWER:
Four WHORLS CALYX (sepals), COROLLA (petals), ANDROECIUM (Male

reproductive organ), GYNOECIUM (Female reproductive organs).
Male Reproductive Organ

Andro
ecium consists of Stamens.
Stamen consists of anther, filament & connective (when anther is bilobed)
Anther bilobed
has 4 Microsporangia.
Refer fig. 2.1 of NCERT (L.S. OF A FLOWER WITH DIFFERENT PARTS)
MICROSPOROGENESIS:
Microspore mother cell (2n)
Meiosis
Microspore (n)
Mitosis
Pollen grains (n)
Pollen grains have two outer walls; i) Exine ii) Intine

Exine is made of sporopollenin (HARDEST
NATURAL SUBSTANCE)
Mature pollen grains have two cells
big vegetative cell & small generative cell.
Generative cell forms two male gametes by
mitotic division.
Pollen grains shed in-celled/3celled
2
stage2
GYNOECIUM / CARPEL (THE FEMALE REPRODUUCTIVE ORGAN)

Refer fig 2.7 of text book (Structure of anatropous ovule)
Ea
ch Carpel consists of ovary, style & stigma.
Ovules are attached to ovary by placenta.
The
funicle stalk of ovule
Hilum, a region where funicle is attached
Integuments
cover embryo sac.
Micropyle
a pore for entry of pollen tube & to imbibe water
MEGASPOROGENESIS
Megaspore mother cell (2n)
Meiosis
4 Megaspores (n)
(3 megaspores degenerate, 1 remains functional)
Funtional Megaspore (n) (Divides 3 times by mitosis)
8 Nucleated Embryo Sac formed
3 cells group at micropylar end -the egg cell(n) & 2 synergids(n)
3 cells at chalazal end called antipodals(n)
2 polar nuclei at center(n each )
Ref fig 2.7 and 2.8 of text book
POLLINATION transfer of pollen from anther to stigma. Agents of pollination air, water,
insect. bat, bird, man.
Transfer of pollen
to stigma
Self pollination
(Autogamy)
Cross pollination (Hetrogamy)

Geitenogamy
Auto gamy
(Same flower)
Xenogamy (different flowers on

different plants of the same
species)
Different flowers
same plant
Double fertilization
Pollen grains germinate on stigma

& pollen tube grows through style.
Pollen tube reaches micropyle
& releases two male gametes into embryo-sac.
Fertilisation is the process of fusion of male
& female gametes (n+n) to form a diploid(2n) zygote.
SYNGAMY: Fusion of one male gamete(n) with egg (n)
Zygote(2n)Produced
First Fusion
Fusion of two Polar Nuclei(n+n=2n)
Second fusion.
Male Gamete(n) Fuses with the fusion product of the two polar nuclei(3n)
Third Fusion
fusion of male gamete with egg cell.
DOUBLE FERTILIZATION
: i)Fusion of male gamete with egg First fertilisation,
ii)Fusion of fusion product of polar nuclei with male gamete Second fertilisation.
10
Refer fig 2.13 in NCERT
POST FERTILISATION CHANGES:

STAGES OF EMBRYO DEVELOPMENT AFTER FERTILISATION
1. Zygote divides by mitosis into suspensor & embryo cells
2. Suspensor cell forms a globular basal cell which remains embedded in the endosperm & a
multicellular suspensor bearing the embryo
3. Globular embryo becomes heart-shaped & then mature embryo with radicle, plumule & Cotyledons
Primary endosperm nucleus
divides repeatedly to form endosperm, food for the embryo.
Mature ovary becomes fruit.
Mature ovule becomes seed.
True Fruit develops only from the ovary, e.g. mango, tomato
False Fruit develops from parts of the flower other than the ovary e.g. apple, peach etc.
Seeds two types: i) Albuminous (with Endosperm)
ii) Non albuminous(without Endosperm)
Special mechanism of reproduction:I) Apomixis- Production of seeds without fertilisation e.g. species of Astereceae and grasses.
ii) Polyembryony- Occurrence of more than one embryo in a seed e.g.Orange.
OUTBREEDING DEVICES:
Continued self-pollination result in breeding depression. Flowering plants have developed many
devices to discourage self-pollination & encourage cross-pollination such as
Bearing unisexual flowers
Anther & stigma mature at different times
Anther & stigma placed at different positions
Self-incompatibility where pollen grains of a flower donot germinate on the stigma of the same
flower
ARTIFICIAL HYBRIDISATION:
Types of cross-pollination performed by man for crop improvement. Achieved by
i) Emasculation i.e. removal of anthers from the flower bud of a bisexual flower before the anther dehisces
using a pair of forceps and
ii) Bagging i.e. covering the emasculated flowers with a bag of suitable size to protect them from
contamination with unwanted pollen
If flower is unisexual, emasculation is not needed. Flower bud bagged & when the stigma becomes
receptive, pollination is done using desired pollen & the flower is rebagged
11
Questions
Short Answer Type Questions (3 marks.)
1. Explain the phenomenon of double fertilization in angiospermic plants.
2. Trace the development of the mature ovule from a megaspore mother cell.
3. Enumerate the steps in artificial hybridisation.
4. Differentiate between monoecious and dioecious plants, with an example for each.
5. How is pollination effected in Vallisneria?
Long Answer Type Questions (5 marks).
1. Represent diagrammatically the formation of an embryo sac from a megaspore mother cell.
2. Draw a well-labelled diagram of the L.S of embryo of grasses. How does it differ from that of bean.
12
Chapter-3 HUMAN REPRODUCTION

Ref.: Concept map: Page-C1 & C2
The Male reproductive system

1. Penis
a. Urination
b. Sexual intercourse
1. Corpus cavernosum- spongy tissue that fills with blood to make penis erect
2. Glans- the head, end of penis
3. Foreskin
i. Covers glans,
ii. May be removed surgically in an operation (circumcision)
2. Scrotum
a. Located behind penis

b. Contains two testes
c. Temperature sensitive (Sperm must be made in cooler conditions i.e, 2-3 C
lower than body temperature)
3. Testes
a. Sperm is produced by the seminiferous tubules due to FSH
b. Testosterone is produced by Leydig cells due to LH
1. Causes the development of the male sex organs at ~8 weeks after
conception.
2. Responsible for facial, armpit, and pubic hair, bone growth and muscular
development.
c. Testes formed in the abdomen before birth. Descend through the "inguinal
canal" during fetal or post-natal life. Sometimes it may take months/years to
reach right place. Possible site for hernia.
4. Epididymis:
Stores sperm until they have matured.
5. Vas deferens:
Tube that leads from the epididymis to the urethra.

Many sperm cells are stored here too.
6. Prostate gland:
Provides an alkaline fluid that can protect sperm from harsh vaginal acids.
7. Seminal
Vesicles:
Produce food for sperm. Food "Fructose"
8. Cowper's gland:
Produces clear lubricating fluid
13
The Female Reproductive System

Ovary:
i) Each ovary contains immature ova (eggs) in follicles.
ii) Females born with lifetime supply of eggs(250,000-400,000 in each ovary)
iii) Ovaries release ovum -. Almost all ova degenerate between birth and puberty.
iv) Approx. 400 eggs will be ovulated over woman's life.
v) Egg is the largest human cell.
vi) Ovaries are located lower abdomen. 1 left and 1 on the right.
Fallopian tubes
i) Two thin tubes attached to the upper sides of uterus
ii) Tubes terminate near the ovaries but are not attached
iii) "Fimbriae" are finger-like structures on the end of each tube
iv) Tubes conduct egg to uterus by use of small hairs called "cilia"
v) Fertilization of ovum takes place in the ampullary-isthmic junction of the fallopian tubes. Egg viable
for only 24-48 hours after ovulation.
Uterus:
i) Pear-shaped organ located in lower abdomen
ii) Muscles (myometrium) stretch to allow baby to develop. Oxytocin starts labor contractions.
iii) Lining of uterus (endometrium) thickens with blood-rich tissue due to progesterone
iv) Endometrium supports embryo/fetus during growth
v) Placenta It is the interface between baby and mother. If not pregnant, lining breaks down and is
discharged from body through vagina. This is menstruation (period)
vi)Cervix connects uterus to vagina. Like a door that opens during ovulation. Cervical mucous closes the
door at all other times.
Vagina:
Birth canal:
i)Menstrual blood leaves the body
ii) Organ of intercourse
iii) Muscular stretches to allow a baby to grow
14
iv)Vaginal opening partly remains closed by thin membrane of tissue called hymen. May be stretched or
torn during any physical activity
Cervix:
i)Located at inner end of vagina
ii)Opening of uterus into vagina
iii) Mucous prevents bacteria and viruses from entering uterus
iv)Lets sperm into uterus after ovulation
v) Where baby also passes through during vaginal birth
Labia:
2 layers of skin, which fold over the opening to vagina and urethra
ii) Inner labia (labia minora)
iii)Outer labia (labia majora)
1.Two folds of skin, surround vaginal area
2. Pubic hair grows on outer labia
Clitoris:
i) Small organ, 5 to 10 millimeters long
ii) Located at junction of inner labia near front of body
iii) Contains erectile tissue & sexually sensitive
Mons pubis :Cushion like fatty tissue covered by skin and pubic hair
GAMETOGENESIS & ITS HORMONAL REGULATION :
Ref: Concept Map Page C 3
Differentiate between: Spermatogenesis and oogenesis :

Spermatogenesis
Oogenesis
Produces male gametes (sperm)
produces female gametes (oocytes)
occurs in the seminiferous tubules (in testes)
occurs in the ovaries
involves meiosis
occurs throughout life after puberty
involves meiosis
occurs after puberty until menopause
may produce 400,000,000 per day
humans normally produce one oocyte during

each ovarian cycle
Primary spermatocyte divide equally to form two

similar secondary spermatocytes
Primary otocyte divide unequally to form one

large secondary oocyte and a small polar body
One spermatogonium produces 4 functional

spermatozoa
An oogonium produces one functional ovum and

3 non functional polar bodies
15
2)Follicular phase (Proliferative phase ) and Luteal phase (Secretory Phase)
Proliferative phase
Secretory Phase
Follicular phase
Luteal phase
Stage of repair and proliferation
Prepares endometrium for implantation
It extends from the end of menstruation to ovulation
It extends after ovulation to menstruation
LH and FSH increases
LH is high (LH surge)
Estrogen level increases
Progesterone level increases
Estrogen is secreted by Graffian follicle
Progesterone secreted by corpus luteum
Menstrual Cycle
Menstruation
Repair of the
endometrium
Follicular Phase
Ovulation
Due to LH
FSH/Estrogen
Thickening of the
endometrium
Breaking
down
Luteal Phase
LH/Progesterone
MENSTRUAL CYCLE: Ref. Concept Map Page C 4
16
Functional Activities of FSH and LH
17
FUNCTION
/ PRODUCTION
FSH and LH from the pituitary:

Hormone
In Females
In Males
FSH Controls
Eggs + Estrogen
Spermatogenesis
LH Controls
Ovulation +
Corpus Luteum
Testosterone
DEVELOPMENT OF OVARIAN FOLLICLE

From Primordial Follicle to Tertiary Follicle
Primordial follicle: The surviving primary oocytes, at birth, are surrounded by thin, single layers cells of
so-called follicular epithelial cells.
Primary follicle
The primordial follicles while developing into primary follicles the follicular epithelium that surrounds the
oocyte becomes iso- to highly prismatic
A
B
1
2
Primordial follicle
Primary follicle
Oocyte
Follicular epithelium
1
2
3
4
18
Oocyte
Pellucid zone
Stratum granulosum
Theca folliculi cells
Secondary follicle
Secondary follicles with follicular epitheliums encompassing multiple rows are formed called the stratum
granulosum. Pellucid zone, between the oocyte and follicular epithelium becomes visible.
Tertiary follicle
A well-developed net of capillaries in the theca internal.

Antrum a fluid filled cavity develops
The theca layer organized in to Theca internal & Theca external
Tertiary follicle
EMBRYONIC DEVELOPMENT
Ref.: Concept Map- C5
1
2
3
4
5
6
7
8
Oocyte
Pellucid zone
Stratum granulosum
Theca internal
Theca external
Antral follicle
Cumulus oophorus (Granulosa cells, together with the oocyte)
Basal lamina between theca and stratum granulosum
Conception to Birth
The following shows some of the many stages of human development:
Zygote
The single cell that results from

fertilization of an ovum by a sperm.
Morula
*The morula (little mulberry)I

Solid ball (16 64 cells).
Morula arises from mitotic
(cleavage) divisions.
19
The blastocyst is a liquid-filled ball of

cells. Occurs around 5 8 days after
conception. Implantation in the
endometrium occurs at this stage.
Blastocyst
Embryo
Human considered an embryo from

implantation until about 8 weeks after
conception.
Foetus
8 weeks after conception until birth.
20
Fate of three germ layers

Ectoderm
Mesoderm
Endoderm
Nervous system
Skeleton
Digestive tract
Epidermis of skin
Muscles
Respiratory system
Circulatory system
Liver, pancreas
Gonads
Bladder
Mnemonics
Tubules in male reproductive system
SEVEN UP
Menstrual Cycle
FOL(d) M(a)PS
Ovarian cycle:
Follicular phase
Ovulatory phase
Luteal phase
Seminiferous tubules
Epididymis
Vas deferens,
Ejaculatory duct
(Nothing)
Urethra
Penis
Menstrual cycle:
Menstrual flow,
Proliferative phase
Secretory phase
21
TERMS TO REMEMBER
Acrosome- the part of a sperm cell that contains an enzyme (This enables a sperm cell to penetrate an egg.)
Afterbirth-placenta and fetal membrane expelled from the uterus after the birth of a baby
Amniotic sac-fluid-filled membrane or sac that surrounds the developing embryo while in the
uterus.(protects baby from hard shocks, keeps it at a constant temperature. )
Birth-the process of being born. process by which baby moves from the uterus into the outside world.
Parturition
Blastocyst = blastula = early stage of an embryo; a liquid-filled sphere whose wall is composed of a single
layer of cells; during this stage (about eight days after fertilization) implantation in the wall of the uterus
occurs.
Cervix- lower part, or neck, of uterus. (Opening to the uterus.)
Clitoris-small, sensitive organ in front of the vagina
Coitus-synonym for sexual intercourse
Conception-fertilization of an egg cell by a sperm cell
Corpus Luteum - After ovulation, this crater-like structure produces progesterone and estrogen. The
corpus luteum is the old RUPTURED GRAFFIAN follicle. It means yellow body.
Cowper's glands - 2 glands that secrete an oily liquid, which cleans and lubricates the urethra of the
male
Egg cell-a female sex cell (female gamete or ovum or secondary oocyte )
Ejaculation-the discharge of semen from the penis
Embryo-the unborn child developing in the uterus between the second and eighth weeks of life
Endometrium - the lining for the uterus site for blastocyst implants and develops.
22
Epididymis-the tightly coiled tube at the back at each testis that holds newly created sperm (each
epididymis is like a nursery where sperm mature and learn to swim.)
Erection- condition of penis when it fills with blood and becomes firm, enlarged, and erect.
Fallopian tubes -tubes leading from each ovary to the uterus. Tubes carry the egg from ovary to the
uterus. Fertilization occurs here. (Also known as the oviducts.)
Fimbria - A fimbria (plural fimbriae) Latin word, literally means "fringe." a fringe of tissue near the ovary
leading to the fallopian tube
Fertile- able to conceive a child
Fertilization union of sperm and egg. Conception.
Fertilized egg- egg after sperm has united with it. Zygote
Fetus-unborn child developing in the uterus after the first eight weeks of life
Follicle - In ovaries. Each holds and nourishes an egg until ovulation. Nest. Becomes corpus luteum after
ovulation.
Foreskin A sheath of skin that surrounds the penis.
Follicle Stimulating Hormone - secreted from pituitary gland in both men and women. In women, FSH
promotes the development of eggs and estrogen. In men, FSH promotes the development of sperm cells.
Fraternal twins-babies that develop from two eggs, each fertilized by a sperm cell; may or may not be of
the same sex
Gamete a sex cell. Sperm cells and egg cells are gametes.
Genitals-the reproductive or sex organs, especially the external organs
Germ Cell- egg or sperm cell. Gamete. In humans, germ cell contains 23 chromosomes
Hormones-chemical substances produced by the endocrine glands; they act in other parts of the body
and affect maturation, growth, and behavior; LH, FSH, GH, Testosterone, Estrogen, Progesterone are all
hormones.
Hymen-a thin bit of tissue, or membrane that may partially cover the opening of the vagina
Identical twins-babies that develop from a single fertilized egg that separates into two halves; identical
twins are always of the same sex
Labia-two folds of skin surrounding the entrance of vagina
Labor-the muscular contractions that expel a baby from uterus during childbirth
Leydig Cells when prompted by LH, Leydig cells create testosterone.
LH - Luteinizing Hormone - Secreted from pituitary gland, causes ovulation and formation of corpus
Luteum in women. In men, LH causes Leydig cells to produce testosterone.
Menstrual cycle - periodic building up and sloughing off of lining of uterus approximately every twentyeight days
Menstruation-the periodic discharge of blood and waste material (unfertilized secondary oocyte / ova
and the degenerating endometrium lining) from the uterus
Miscarriage- expulsion from the uterus of a fetus before it is developed sufficiently to live Also called
spontaneous abortion
Myometrium muscles of the uterus that stretch to accommodate the growth of the baby. These
muscles contract during labor and push baby out
Orgasm-the peak of sexual excitement when the male ejaculates semen,
Ovary- female sex glands; they produce egg cells, estrogen and progesterone.
Ovulation-the discharge, or release, of an egg cell / secondary oocyte from the ovary
Ovum-scientific name for an egg cell / secondary oocyte
Oxytocin - hormone, released from the pituitary gland , stimulates contraction of the myometrium of the
uterus during labor and facilitates ejection of milk from the breast during nursing.
23
Penis-the male sex organ through which sperm cells leave the body; it also discharges urine
Placenta- network of blood vessels and other tissues by which the unborn child is attached to the wall of
the uterus. grows out of the endometrium The umbilical cord is attached to it. It is the interface between
mother and developing fetus.
Pregnancy-the process in a woman from conception to birth
Pregnant-the condition of a woman with an embryo or fetus in her uterus
Progesterone pregnancy hormone, which is first produced by the corpus luteum and then by the
placenta.
* increases lining of endometrium.
*maintains pregnancy
*helps develop mammary glands.
Prostate gland- surrounds the upper end of male urethra and produces part of the fluid that mixes with
the sperm to form semen. Prostate fluid alkaline ,helps to protect sperm from vaginal acids.
Scrotum- pouch of loose skin containing the testes. Houses and air-conditions the testicles by moving
and sweating.
Semen-the mixture of sperm and fluids released during ejaculation. Semen comprised of sperm, fructose,
prostate fluid and oil from Cowpers gland.
Seminal vesicles-small saclike organs opening into each vas deferens near the upper end of the urethra;
produce part of the fluid that mixes with the sperm to form semen; provide food (fructose) for the sperm.
Seminiferous tubules - tubes in testes that produce sperm
Sexual intercourse- entry of penis into vagina and subsequent release of semen; also called coitus
Sperm-the male sex cell (male gamete or spermatozoon), which contains 23 chromosomes in human.
Spermatozoon-scientific name for sperm
Spontaneous abortion--synonym for miscarriage
Testes- male sex glands; produce sperm cells and testosterone;
Testicles-synonym for testes
Testosterone male hormone that regulates development of penis, muscles, body hair, etc
Umbilical cord- ropelike structure connecting embryo or fetus to placenta within the uterus.
Urethra-the tube through which urine is expelled from the bladder in both males and females and through
which semen leaves the male body
Uterus = womb - the hollow pear-shaped organ in which a baby develops before it is born;
Vagina- passage from the uterus to the outside of the body , accepts the penis during intercourse. It is
the birth canal & menstrual fluids leave through it
Vas deferens- tube extending from each epididymis to the urethra in males
Womb-synonym for uterus
Yolk sac-a structure that develops for the nutrition of embryo during early embryonic life and then ceases
to function
Zygote - The cell formed by the union of two gametes. [ fertilized ovum before cleavage.]
24
IMPORTANT NOTES
An adult male produces over 1012 to 1013 sperms each day
Human female oogonial development by mitosis is completed by 25 weeks of foetus and no
oogonia are formed after birth
Sperm entry into the ovum stimulates MPF (M phase promoting factor) & APC ( Anaphase
promoting complex) for completion of Meiosis II
During Spermatogenesis, spermatogonium produces four sperms while in oogenesis, oogonium
produces one ovum and two polar bodies.
Human Sperm contains Clupein proteins.
Yolk nucleus: A mass of mitochondria and golgi bodies near nucleus is called as yolk nucleus and
controls vitellogenesis.
Maximum level of estrogen 12th day, LH-13th day, Progestrone 21st day, Corpous luteum
formation 19th day of menstrual cycle.
Menstrual cycle is absent during pregnancy, lactation periods and permanently during menopose.
Two ovaries alternate in ovulation
13 mature eggs are released per year, so about 416 eggs (13x32 years) are ovulated during whole
reproductive period of human female.
Menstruation is also called Weeping of uterus for the lost ovum or Funeral of unfertilized eggs.
In human embryo, yolk sac degenerates since eggs is microlecithal, which shows evolutionary
significance.
Placenta acts as a physiological barriers and an ultra-filter between foetal and maternal blood.
Progesterone is also called pregnancy hormone since its secretions controls pregnancy
Teratogens are physical, chemical, biological agents which may cause malformation in developing
embryo
Period between fertilization and parturition is called gestation periods. Varies between 266 days up
to 280 days ( 49 weeks)
25
Chapter-4: REPRODUCTIVE HEALTH

Reproductive Health:
Human reproductive health and sexuality involve great many components and interrelationships.
A total view of human reproductive health is basic to personal well-being as well as to interpersonal
relationships.
Every individual is a unique sexual being.
Adolescents are vibrant, fragile and prone to experimentation and risk taking, as a result they are the
most vulnerable population as far as delinquent behavior and attitude is concerned.
Every decision has its own consequence. Any wrong decision can lead to disastrous consequence,
which in turn can ruin ones life.
Sexual adjustment is part of total personality adjustment. Self-esteem is the key to sexual maturity.
Broad based community and institutional support for reproductive health is essential.
Adolescence Reproductive and Sexual Health (ARSH) topics are to be taken care of to dispel the
myths and misconception about this important aspect with focus on:
reducing risky behavior
theories which explain what influences people's sexual choices and behaviour
reinforced message about sexual behaviour and risk reduction
Providing accurate information about, the risks associated with sexual activity, about contraception
birth control, methods of avoiding or deferring intercourse
Dealing with peer and other social pressures on young people; providing opportunities to practice
communication, negotiation and assertion skills
Uses a variety of approaches to teaching and learning that involve and engage young people and
help them to personalise the information
Uses approaches to teaching and learning which are appropriate to young people's age,
experience and cultural background
Methods of birth control

CONCEPT MAP Ref: CH-4 (Page-3)
Behavioural methods: Behavioural methods depend on a good knowledge of the menstrual cycle
as well as adequate self control by the couple.
o Coitus Interruptus: Coitus interruptus means 'interrupted sex". In this birth control
method, the penis is withdrawn from the vagina just before ejaculation.
The main advantage is that this method does not require the use of any drug, does not
interfere with normal body functions, and the couple can plan for pregnancy at any time
26
they want. The main disadvantage is that it is dependent almost wholly on the man's selfcontrol. The failure rate is high at 15 - 18%.
o Rhythm method or Safe Period: This method requires a good knowledge of the female
partner's menstrual cycle to identify the days on which sexual intercourse is possible
without the risk of pregnancy.
Avoiding vaginal Intercourse: Anal sex, oral sex or sex without penetrating the vagina
Barrier methods: In barrier methods of birth control, a barrier is placed between the penis and the
vagina during intercourse so that the sperm cannot meet the ovum for fertilization.
o Male Condoms:, usually made of latex that covers the erect penis during penetration of
the vagina.
o Female Condoms: made of polyurethrane, loose sheath with two rings on either side.
can be inserted about 8 hours prior to sexual intercourse and can be kept in for about
another 12 hours after intercourse. Can be used more than once during this period.
o Condoms protect against pregnancy as well as sexually transmitted diseases
(STDs), including HIV/AIDS.
o Diaphragm: vaginal is a small saucer shaped rubber sheath with a metal coil in its rim
which is fitted across the mouth of the uterus (cervix).
o Cervical Cap: The cervical cap is a small dome-shaped rubber device fitted on the cervix.
It is uncomfortable to apply and is rarely used nowadays.
o Vaginal Sponge: The sponge is a small polyurethrane round device which needs to be
placed inside the vagina before sexual intercourse. It releases spermicide which makes
sperm inactive. It should be left in place for 8 hours after use and can be used more than
once during this time.
The sponge also acts as a barrier contraceptive to some extent since it swells up to fit
across the cervix once it is inside the vagina.
Hormonal Methods: Drugs are used to either prevent ovulation or to prevent implantation of the
embryo after fertilization.
Combined oral contraceptives contains two hormones similar to the natural hormones in a
womans body---an estrogen and a progestin.
How the Birth Control Pill works

Mainly work by preventing ovulation. In a normal menstrual cycle, the pituitary gland secretes the hormones
FSH and LH to stimulate the ovary to release an egg ('ovulation").
Progesterone in pills make the cervical mucus hostile to the sperm.
Causes changes that make the endometrium unreceptive to a fertilized ovum if ovulation and
fertilization do take place
o Oral Contraceptive pills: Combined oral contraceptive pills or birth control pills contain
two hormones - estrogen and progesterone. They have two functions. The main one is to
prevent ovulation. The second function is to disrupt the normal growth of the internal
uterine lining (endometrium) so that the embryo cannot implant in it.
27
Centchroman: This is a non-hormonal non steroidal contraceptive. The main function is to

cause a slowing down in the growth rate of the internal uterine lining as well as to speed
up the movement of the embryo so that implantation cannot occur.
The Patch: The patch (Ortho Evra). This is a thin band-aid like patch containing estrogen
and progesterone should be applied over the skin. Releases the hormones slowly into the
skin through which they are absorbed.
Depo-provera: This birth control method consists of injecting a high dose of the hormone
progesterone every three months. It acts mainly by preventing ovulation. The main
disadvantage is that there may be irregular bleeding throughout the three months.
Nuvaring: This is a thin silastic ring which should be inserted into the vagina once every
month. It releases the hormones estrogen and progesterone and prevents ovulation during
the menstrual cycle.
Subdermal Implants
The Norplant (a registered trademark of The Population Council for levonorgestrel subdermal
implants) Implant system set of six small plastic capsules. Capsules placed under the skin of a
womans upper arm.
Norplant capsules contain aprogestin, similar to natural hormone that a womans body makes. It is
released very slowly from all six capsules. Thus the capsules supply a steady, very low dose.
Norplant implants contain no estrogen.
Norplant capsules thicken cervical mucus making it difficult for sperm to pass through. It stops
ovulation (release of eggs from ovaries) in about half of the menstrual cycles after the first year of
use.
Emergency Oral Contraception
After unprotected sex, emergency oral contraception can prevent pregnancy. Sometimes called
postcoital or morning after contraception.
Mainly stops ovulation
Regular use of emergency contraceptives has serious health hazards.
Vaginal Pessaries, Tablets, Creams or Foams:
These contain spermicides which are toxic to the sperm and should be inserted into the vagina just
before coitus. Their advantages are that they are easy to apply, do not interfere with coitus and act
as lubricants. Disadvantage is that they are not very effective always.
Intra-Uterine Contraceptive Devices (IUCD):
IUCDs or IUDs are contraceptive devices which are placed inside the uterus. Small, flexible plastic
frame. Has copper wire or copper sleeves on it. Inserted into a womans uterus through her vagina.
Have two strings, or threads, tied to them. Strings hang through the opening of the cervix into the
vagina. A provider can remove the IUD by pulling gently on the strings with forceps.
Preventing sperm and egg from meeting. Perhaps the IUD makes it hard for sperm to move through
the womans reproductive tract, and it reduces the ability of sperm to fertilize the egg. Prevent the
egg from implanting itself in the wall of the uterus.
IUCDs prevent pregnancy by making the endometrium unreceptive to the fertilized ovum. It
stimulates the endometrium to release leukocytes (WBCs) and prostaglandins making it hostile to the
sperm. It also causes bizarre and irregular growth of the endometrium. This prevents implantation of
28
a fertilized ovum.
IUDs like Copper-Ts also come wrapped in copper. Copper is toxic to sperms and is a method of
enhancing the contraceptive effect of the IUDs.
The IUCDs can come in various shapes and sizes.
Lippes Loop: The Lippes loop consists of a thin plastic (or polyethylene)wire bent in a series of Sshapes.
l
Lippes loop
Copper-Ts
Copper T: T-shaped structure which stays inside the uterus with the long arm of the T along the
uterine cavity (endometrium) and the shorter arms transversely across the upper part of the
endometrium.
Mirena: Releases a progesterone called levonorgestrel. It works by affecting ovulation, affecting
the normal growth of the endometrium and by affecting the cervical mucus so that the movement of
sperm is obstructed. In the United Kingdom, hormone based IUDs are known as Intra-uterine
Systems (IUS).
Surgical Methods: These are more
or less permanent methods of
contraception.
o Tubal Ligation: Both the
female tubes are tied off
and usually cut during tubal
ligation to prevent the
sperm from reaching the
ovum during intercourse.
o Vasectomy: The two tubes
which carry sperm from the
testes to the penis are the
vas deferens. Tying them
29
off and cut.

Essure: Essure is a method in which small micro-inserts are placed at the mouth of the
fallopian tubes to cause scarring and block them. This prevents sperm from reaching the
ovum for fertilization.
HOW PREGNANCY OCCURS

Occurs when a sperm with an ovum.
Ovum round about 100 microns in diameter, The ovum is picked up by the fallopian tube on the same side.
The tubes have long fingerlike projections called fimbria which it uses, rather like hands to pick up the
ovum. Ovum then moves through the tube, propelled along by long hairs growing from cells in the tubes.
Like grass bending before the wind, the hairs bend towards the uterus in waves, pushing the ovum slowly
towards the uterus. The egg remains viable, (alive) for about 72 hours, but is capable of being fertilized for
only about 12 - 24 hours. If it remains unfertilized during this period, it disintegrates in the tube without
leaving any trace. Its end products (mainly proteins) are absorbed into the bloodstream and excreted
through the urine or stool.
Fertilization, Implantation and Pregnancy

The sperm viable for a longer period, found in uterus 5 7 days after coitus. But capable of fertilizing an
ovum for only 48 - 72 hours after being ejaculated. Time taken by sperm to reach tubes is between 6 12
hours but many authorities say it can be as early as 1 hour.
Intercourse has to take place within this narrow time frame (1-2 days before ovulation or immediately after
ovulation), for a pregnancy to occur. At every intercourse normal man deposits 2 5 mililitre of semen in
the upper part of the vagina (see diagram). Each mililitre of semen normally contains about 50 200 million
of sperms.
30
Sperms swim rapidly upwards into the uterus and from there into the two tubes on either side at the rate of
3mm per hour. Takes an average of 10 hours for sperm to reach tubes.
All the sperms deposited in the vagina cannot swim into the uterus. Only about 1 % of the total number of
sperms deposited in the vagina make the journey.
Hundreds of sperm (estimated to be around 300) surround the ovum in the tube. They press against the
membrane of the ovum attempting to penetrate it and fertilize the ovum. Finally one sperm succeeds. At
once a chemical reaction is triggered off in the wall of the ovum, making it impenetrable to any other sperm.
No other sperm can enter the ovum now.
Unsuccessful sperms slowly degenerate, break down and become indistinguishable from any other protein
end product in the female partners body (sperm made up mainly of protein). These get absorbed into the
blood stream, are carried away to be expelled from the body in the stool or the urine.
Sperm that manages to penetrate the ovum fuses with it to form a single cell called a zygote. Zygote starts
to divide as it is propelled towards the uterus dividing first into 2 cells, then into 4, then 8 and so on.
Dividing zygote called an embryo.
When the process of in-vitro fertilization (IVF) is carried out, the ovum and the sperm is allowed to fertilize
in a laboratory dish (petri dish). The embryo is usually transferred into the mothers uterus at the 4 8
celled stage, usually on the third day after fertilization.
By the time the zygote reaches the uterus at about the 6th to 9th day after ovulation, it is a 16-celled cluster
of cells called a morula. The morula looks rather like a bunch of grapes. Each of its cells is identical to each
other.
Theoretically, it is from the 2-celled to the 16-celled stages that the cells can be separated from each other
and allowed to develop into clones of each other (identical twins). In nature, separation occurs
spontaneously usually at the 2-celled stage to form identical twins. Separation at later stages can lead
to the potentially fatal condition of conjoint twins or Siamese twins.
31
When it reaches the uterus, the morula sticks to the inner lining of the uterus (called the 'endometrium'). By
this time, the hormone progesterone released by the ovaries finishes preparing the endometrium to receive
the morula.
The morula burrows deep into the endometrium and by the 9th - 12th day after ovulation, is fully buried in it.
Burrowing can cause a little bleeding called implantation bleeding. Buried embryo begins to develop, Its
cells increasing in number and gradually becoming different from each other in the way they function.
The development of the embryo continues until at the end of 9th month of pregnancy (40 weeks or 280
days), a fully formed baby, capable of leading a life independent from its mother is ready to be born.
INFERTILITY AND ITS TREATMENT

Infertility when a couple fails to conceive after one year of sexual life without contraception. 80% of all
women desiring children, conceive within 1 year of marriage and another 10% within the second year.
According to the World Health Organisation, incidence of infertility is about 10 % worldwide. Another 1012% of all the other couples have only one child and wish to have more. The incidence of infertility is
gradually increasing all over the world.
For many people going through infertility treatment, the level of distress and tension can be very high.
Mutual trust and faith in the doctor can help the couples to enquire about different modalities of treatment
for both the male as well as the female partner, and make informed decisions of their reproductive status
azoospermia (complete absence of sperm). In these cases, sperm has been aspirated from the testes
directly.
Intra-Uterine Insemination (IUI): This method is used in men with moderately low sperm count.
the semen is collected by masturbation, washed and centrifuged to increase the sperm density.
This high density sperm sample is now injected into the uterus, bypassing the vagina. The chances
of hostile cervical mucus is thus eliminated. Since the sperm is injected into the uterine cavity,
chances of pregnancy is increased. The procedure should be done within 2 hours of collecting the
semen.
o Insemination with Husband's Semen (AIH) This is done in cases of impenetrable cervical
mucous or when disease or deformity of the cervix makes it impossible for the sperm to enter the
uterus. It is also carried out in cases of impotence or premature ejaculation.
o
Insemination with Donor Semen (AID) AID is usually carried out in couples where the husband
suffers from azoospermia.
InVitro Fertilisation (IVF) process by which egg cells are fertilised by sperm (usually 100,000 sperm / ml)
outside the womb, in vitro. IVF is a major treatment in infertility when other methods of assisted
reproductive technology have failed. The process involves hormonally controlling the ovulatory process,
removing ova (eggs) from the woman's ovaries and letting sperm fertilise them in a fluid medium. The
fertilised egg (zygote) is then transferred to the patient's uterus with the intent to establish a successful
pregnancy. The first successful birth of a "test tube baby", Louise Brown, occurred in 1978. Prior to that,
there was a transient biochemical pregnancy reported by Australian Foxton School researchers in 1973
and an ectopic pregnancy reported by Steptoe and Edwards in 1976.
32
In vitro, (Latin) meaning within the glass, biological experiments involving cultivation of tissues outside the
living organism were carried out in glass containers such as beakers, test tubes, or petri dishes. Term in
vitro used to refer to any biological procedure that is performed outside the organism. In vivo procedure,
tissue remains inside the living organism within which it is normally found. A colloquial term for babies
conceived as the result of IVF, test tube babies, However, in vitro fertilisation usually performed in shallow
containers called Petri dishes. (made of glass or plastic resins.)
Zygote intrafallopian transfer (ZIFT) infertility treatment where blockage in the fallopian tubes prevents
the normal binding of sperm to the egg. Egg cells removed from woman's ovaries, and in vitro fertilised.
Resulting zygote placed into the fallopian tube by laparoscopy. The procedure spin-off of the gamete
intrafallopian transfer (GIFT) procedure. ZIFT has a success rate of 64.8% in all cases.
Takes an average five weeks to complete a cycle of ZIFT. First, the woman must take a fertility medication
to stimulate egg production in the ovaries. The doctor will monitor growth of ovarian follicles, once they are
mature, woman will be injected with human chorionic gonadotropins (hCG). Eggs will be harvested
approximately 36 hours later, usually by transvaginal ovum retrieval. After fertilization in laboratory resulting
early embryos or zygotes are placed into the woman's fallopian tubes using laparoscope.
Gamete intrafallopian transfer (GIFT) assisted reproductive technology against infertility. Eggs removed
from a woman's ovaries, placed in one of the Fallopian tubes, along with the man's sperm. The technique,
which was pioneered by endocrinologist Ricardo Asch, allows fertilization to take place inside the woman's
body.
Takes, an average of four to six weeks to complete a cycle of GIFT. First, the woman must take a fertility
drug to stimulate egg production in the ovaries. The doctor will monitor growth of ovarian follicles, once they
mature, woman will be injected with Human chorionic gonadotropin (hCG). The eggs will be harvested
approximately 36 hours later, mixed with the man's sperm, and placed back into the woman's Fallopian
tubes using laparoscope.
Intracytoplasmic Sperm Injection (ICSI): technique in which a single sperm injected into the centre of the
egg, in order to achieve fertilization. Sperm is collected from the male partner by masturbation. Single
healthy sperm then injected into the prepared ovum.
The advantage of this method is that only a single sperm is needed - even men with a very low sperm
count can become fathers with this treatment. Men found to be azoospermic, that is with no sperm at all in
the semen, sperm can be suctioned out of the vas deferens ( male tubes). Sperm can also be liberated
from the testes itself by careful testicular biopsy and culture by a method called MESA - Microepididymal
sperm aspiration.
Prevention of Male Infertility : Undescended testes should be treated at the earliest during infancy before
testicular function is damaged. Infections by mumps and other viruses should be managed by keeping a
watchful eye on complications in the testes.
General Facts About STDs
Sexually transmitted diseases (also called STDs, or STIs for sexually transmitted infections): Infections
transferred from one person to another through sexual contact. According to the Centers for Disease
Control and Prevention, there are over 15 million STD cases reported annually in the United States.
33
More than 25 diseases that are transmitted through sexual activity. Other than HIV, the most common
STDs in the United States are chlamydia, gonorrhea, syphilis, genital herpes, human papillomavirus,
hepatitis B, trichomoniasis, and bacterial vaginosis. Adolescents and young adults are the age groups at
greatest risk for acquiring an STD. Approximately 19 million new infections occur each year, almost half
of them among people ages 15 to 24.
Some STDs can have severe consequences, especially in women, if not treated, which is why it is so
important to go for STD testing. Some STDs can lead to pelvic inflammatory disease, which can cause
infertility, while others may even be fatal. STDs can be prevented by refraining from sexual activity, and
to a certain extent, some contraceptive devices, such as condoms.
Specific STDs: An Overview

Human Papilloma Virus: Thought to be one of the main causes of cervical cancer. Has been linked with
other types of cancers of female reproductive system. HPV can be treated to reduce signs and symptoms.
Currently no cure for this virus. HPV vaccine recently developed to prevent HPV infection.
Herpes Virus: STD that presently with no cure. Treatment available. Home remedies & natural treatment
available. Herpes symptoms include blisters or sores that periodically break out on the genitals. Refer
FAQs.
Hepatitis: To cure for those already infected, a Hepatitis B (HBV) vaccine available to prevent spread of
this infection. Many are asymptomatic, however those who do suffer from Hepatitis B symptoms may have
many unpleasant discomforts. Infection may clear up on its own. Some people may suffer from chronic
infections for many years. Treatment available for chronic sufferers. Other types of hepatitis infections that
can be passed through sexual contact include Hepatitis A and Hepatitis C.
HIV/AIDS: Most dreaded STD. New ways of treating this infection significantly prolongs an infected
person's life. For many this infection eventually progresses to AIDS and, ulitmately, death. More than 40
million people worldwide are infected with the HIV virus; women account for 50% of those infected.
Syphilis: Throughout history, cases of syphilis have been recorded. Can easily be treated and cured.
Without treatment, syphilis symptoms can progress and affect the nervous system and brain leading to
dementia and even death.
Trichomoniasis: most common, curable STDs. However, symptoms may be mistaken for a yeast
infection causing women to use wrong type of treatment for her vaginal discharge.
Common Infections:Chlamydia and gonorrhea often infect a person at the same time. Although the
symptoms of chlamydia are different from gonorrhea not unusual for person to be asymptomatic. If testing
for chlamydia, good idea to test for gonorrhea. Both STDs can be cured but can damage reproductive
system if left untreated.
Pubic Lice: Crabs are very similar to head lice. Itchy symptoms can be hard to miss. Treatment for pubic
lice can easily take care of the discomfort these pests can cause.
Rare Infections: Granuloma inguinale and chancroid,. Other lesser-talked about STDs include
nongonococcal urethritis and molluscum contagiosum,
34
KNOW THE SYMPTOMS OF STDs

Men
Swelling or tenderness in genital area.

Blisters ,sores or bumps around the mouth or genitals.
Fever,chills and aches.
Unusual itching.
Burning sensation when you pass urine or move your bowels.
White,watery or yellow disharge from the penis.
Women
Have fewer symptoms than men, often none at all. STDs can lead to cancer. Women should watch
for Bleeding that is not part of their period.
Pelvic or vaginal pain.
Discharge from the vagina.
Painful urination.
Unusual rash, sore or growth in the genital area.
DONT LET STDs TAKE YOU BY SURPRISE
Pelvic Inflammatory Disease Overview
Pelvic inflammatory disease (PID) is infection of a woman's reproductive organs. Infection spreads upward
from the cervix to the uterus, Fallopian tubes, ovaries, and surrounding structures
Pelvic Inflammatory Disease (PID) Symptoms
If a woman has PID, she may have any of these symptoms:

Abdominal pain (especially lower abdominal pain) or tenderness
Back pain
Abnormal uterine bleeding
Unusual or heavy vaginal discharge
Painful urination
Painful sexual intercourse
Symptoms not related to the female reproductive organs include fever, nausea, and vomiting.
PID symptoms may be worse at the end of a menstrual period and during the first several days following
a period.
Ectopic Pregnancy Overview
Pregnancy that develops outside a woman's uterus (womb). When the fertilized egg from ovary does not
implant itself normally in the uterus. Egg develops somewhere else in the abdomen. Such conceptions are
abnormal and cannot develop into a fetus.
Common ectopic pregnancy in fallopian tubes (so-called tubal pregnancy). Also found on the
outside of the uterus, on the ovaries, or attached to the bowel.
Complication of ectopic pregnancy is intra-abdominal hemorrhage (severe bleeding). Eg. tubal
pregnancy the products of conception continues to grow in the fallopian tube, tube expands and
eventually ruptures. This can be very dangerous because a large artery runs on the outside of
each fallopian tube. If the artery ruptures, you can bleed severely.
Ectopic pregnancy usually found in the first 5-10 weeks of pregnancy.
35
INFERTILITY IN HUMAN: Causes & Consequences :IN MALES
IN FEMALES
Oligospermia: Low sperm count
Anovulation: Absence of ovulation.
Azospermia: Absence of sperm.
Oligoovulation: Deficient ovulation.
Asthenozoospermia: Low sperm motility.
Hyperprolactinemia: Ovum remain trapped

inside the follicle.
Teratozoospermia: Defective sperm

morphology.
Idiopathic Infertility: Failure or abnormal

fertilization.
Cryptorchidism:Failure of Testes to descend in

the scrotal sac.
Tubal Infertility: Damaged/ligated fallopian

tube
COMMON SEXUALLY TRANSMITTED DISEASES (STDs)

SL.
NO.
STD
CAUSAL
AGENT
CHLAMYDIOSIS
Chlamydia
trachomatis
GONORRHOEA
Nisseria
gonorrhea
TRICHOMONIASIS
Tricomonas
vaginalis
GENITAL HERPES
Herpes
simplex virus
SYPHILIS
Trepanema
pallidum
GENITAL WART
HEPATITIS-B
Human
papilloma
virus
Hepatitis B
virus
AIDS
HIV
SYMPTOMS
EFFECT ON
FOETUS
EFFECT ON PERSON
AFFECTED
Painful urination
& intercourse
Mucus discharge
from
penis/vagina
Painful urination
in men
Premature
birth,blindness,
Pneumonia
Pelvic inflammatory
disease, Infertility,Ectopic
pregnancy
Still birth,
Blindness
Inflammation,
Itching& vaginal
white discharge
(Leucorrhoea)
Genital sores,
Fever
Not known
Pelvic inflammatory
disease,
Infertility,Rash,Death
Valvar erythema, Burning
dysuria
Still birth, Brain

damage
Cervical cancer.
Initially sores in
genitalia &
mouth,
Rashes
Warts on
genitalia
Premature birth,
Miscarriage, Still
birth
Death
Not known
Cervical cancer
Fatigue, Fever,
Jaundice, Rash,
Abdominal pain
Low birth weight
Liver cirrhosis, Liver

cancer
Fever, Prone to
infection,
Inflammation
AIDS affected
Dementia, Death
36
IMPORTANT NOTES
Indian population is identified as Young population whereas population of USA, England
,Germany etc. are identified as Ageing population.
In India, Kerala has lowest Birth rate & U.P. highest.
Deficiency of Manganese causes infertility & Vitamin E is considered as Antisterility Vitamin.
11th July celebrated as World Population Day.
Every 16th person in the world is an Indian.
Main objectives of National Population Policy, 2000 are : population stabilization, compulsory
school education, reduce infant mortality rate, decrease fertility rate, promote delayed marriage,
incentive for sterilization, restrain child marriage etc.
Kerala declared as the First baby friendly state of the world by first Human Development Report
2002.
I-pills or Intelligent pills are Emergency Contraceptive pills that should be used in emergency
only.Frequent use of it may bring ovarian damage & Menstrual problem.
Contraceptive Corn: Scientists have produced a genetically modified corn crop which produces
antisperm antibodies &suggest that a plant based jelly may be prepared which will prevent
pregnancy & spread of STDs simultaneously.
IMPORTANT TERMS TO REMEMBER
AMNIOCENTESIS: Foetal test based on chromosomal pattern in amniotic fluid surrounding the developing
embryo. can be used for sex determination.
LACTATIONAL AMENORIA: Absence of menstruation due to disruption of ovulation during the period of
intense lactation following parturition.
INTRA UTERINE DEVICES(IUDs): A medical device of insertion of artificial barrier in the uterus through
vagina for obstructing sperm entry.
STERILISATION: Surgical intervention for stopping pregnancy by blocking gamete transport pathway in
male/female.
ASSISTED REPRODUCTIVE TECHNOLOGIES(ART): Artificial technological devices to enable couples to
have children when fail they to get child due to any reproductive disorder.
ARTIFICIAL INSEMINATION: Medical technological devices by which semen collected from a healthy
donor is artificially introduced into the vagina or uterus of female.
ETIOLOGY:Study of causes of diseases.
PROPHYLAXIS: Prevention of diseases.
EPIDEMIOLOGY:Mode of transmission of diseases.
RECANALISATION: Attachment of cut Vasa deferentia with plastic tubes during Vasectomy.
CASTRATION: Surgical removal of Testes.
POPULATION CRASH: Rapid decline in the population.
POPULATION EXPLOSION: Rapid increase in the population.
AGE COMPOSITION: Relative abundance of the organisms of different ages in the population.
IN-VITRO FERTILISATION: Artificial technique of fusion of gametes outside the body in laboratory
condition, in almost similar conditions as that of the body.
INFERTILITY: Inability of a couple to produce children in spite of unprotected sexual cohabitation.
37
Chapter 5 : Genetics
Relationship between genes and chromosome of diploid organism and the terms used to describe them
Know the terms
Terms
Meaning
Example
Locus
Address/ location of a gene in a chromosome
T,A.b,d etc
Allele
Allelomorphs= alternative form of a gene
T and t OR A and a etc
Homozygous
Both alleles of a gene at a locus similar
AA or aa
Heterozygous
Both alleles of a gene at a locus dissimilar
Aa or Tt etc
Homozygous
Dominant
Both alleles of a gene at a locus similar &

dominant
AA
Homozygous
recessive
Both alleles of a gene at a locus similar &

recessive
aa
38
Mendel's first law ( Law of dominance )characters are controlled by discrete units called genes (allele)
which occur in pair. In heterozygous condition only one gene that is dominant can express itself. (Can be
explained by monohybrid cross)
Mendel's second law (Law of segregation): The two alleles received, one from each parent, segregate
independently in gamete formation, so that each gamete receives one or the other with equal probability.
(Can be explained by monohybrid cross)
Mendel's third law (Law of recombination): Two characters determined by two unlinked genes are
recombined at random in gametic formation, so that they segregate independently of each other, each
according to the first law (note that recombination here is not used to mean crossing-over in meiosis). (Can
be explained by dihybrid cross)
This is what Mendel said (summary) :
1) Dominant alleles overpower recessive alleles. Dominant traits overpower recessive traits.
2) Rule of segregation (Separation): Gametes (sex cells) only receive one allele from the original gene.
3) Rule of Independent assortment: One trait will not determine the random selection of another.
Incomplete dominance: When one allele of a gene is not completely dominant over the other and the F1
hybrids are intermediate between two parents. The phenotypic and genotypic ratio is same.1:2:1 in F2
generation. E.g. Snapdragon or Antirrhinum majus
Co dominance: Two alleles of a gene are equally expressive and dominant in a generation eg Human blood
group
( Note : Human blood group is also an example for multiple allelisim i,e when a gene exists in more than
two allelic forms)
Basic outline of Mendels cross
1. Pure breeding parents for a pair of contrasting character (allelic Pair) is taken
e.g, Tall pure-bred pea plants (TT) & short pure-bred pea plants (tt)
2. Gamete formation (Meiosis)
3. Hybridization (crossing is done)
4. F1 generation - the product of the above cross (are called hybrids)
5. Selfing (allowed to self fertilize / self breeding )
6. Gamete formation (Meiosis)
7. F2 generation - the product of the above selfing
8. Analysis of result (Phenotype and Genotype)
39
Linkage
Tendency of genes on same chromosome to remain together
Such genes are called linked genes.
Linked genes present only parental types
Figure Schematic of Genetic Linkage and Recombination

(A) Two homologous chromosomes: blue (paternal) and orange (maternal). Three genes with
separate alleles and linkage " noted (A,a; B,b; C,c;).
(B) Crossing over during meiosis. (
(C) Two alleles and their linked genes have switched locations via recombination. Four additional
alleles and their associated (A,a; B,b;) have not switched and are considered linked.
(D) Recombined haploid chromosomes segregate separately during meiosis as gametes before
fertilization.
(E) Sample recombination frequencies between genes demonstrating higher rates of recombination
for genes further apart.
40
Cross
Result of F2 generation
Monohybrid Tt X Tt
Dihybrid cross
YyRr X YyRr
Incomplete dominance
Rr X Rr
Phenotypic ratio
3:1
9:3:3:1
Genotypic ratio
1:2:1
1:2:1:2:4:2:1:2:1
1:2:1
1:2:1
Co Dominance and multiple allelisim

Blood group
Possible genotype
A
IAIA OR IAi
B
IBIB OR IBi
AB
IA IB
O
ii
Crosses of blood group (CO DOMINANCE)
Blood group
Possible genotype
AXA
IAIA X IAIA
IA IA X IA i
IAi X IAi
BXB
IBIB X IBIB
IBIB X IBi
IBi X IBi
AB X AB
IAIB X IAIB
OXO
ii X ii
Possible phenotype
A
A
A;O
B
B
B; O
AB: A; B
O
POSSIBLE BLOOD GROUP OF PROGENY WITH RESPECT TO THE BLOOD GROUP OF PARENTS
Parent
AXA
AXO
AXB
BXB
BXO
AB X A
AB X B
AB X O
AB X AB
OXO
KEY
Progeny
A
+
+
+
+
+
+
+
-
B
+
+
+
+
+
+
+
+ = POSSIBLE
41
AB
+
+
+
O
+
+
+
+
+
+
+
- = NOT POSSIBLE
Sex determination and sex chromosome

Organism
Male
Human beings
XY
Birds
ZZ
Insects
XO
Female
XX
ZW
XX
Pedigree Analysis
Pedigree is a chart of graphic representation of record of inheritance of a trait through several generations
in a family
Symbols used:- refer NCERT Text Book
Four patterns of inheritance
AUTOSOMAL DOMINANT
1. Traits are controlled by dominant genes
2. Both males and females are equally affected
3. traits do not skip generations
4. e.g. polydactyly, tongue rolling ability etc
AUTOSOMAL RECESSIVE
1. Traits controlled by recessive genes and
appear only when homozygous
2. Both male and female equally affected
3. Traits may skip generations
4. 3:1 ratio between normal and affected.
5. Appearance of affected children from
normal parents (heterozygous)
6. All children of affected parents are also
affected.
7. e.g.- Albinism, sickle cell anaemia etc
A 'typical' autosomal dominant pedigree
x-chromosome linked pedigree
42
Now try to answer

1. Is it possible that this pedigree is for an autosomal dominant trait?
2.
Can two individuals that have an autosomal dominant trait have unaffected children?
3.
Is it possible that this pedigree is for an autosomal dominant trait?
4.
Is it possible that this pedigree is for an autosomal dominant trait?
5.
6.
Is it possible that the pedigree above is for an autosomal recessive trait?

Assuming that the trait is recessive, write the genotype of each individual next to the symbol
7.
8.

Write the genotype of each individual next to the symbol
9.
A = normal
a = the trait (a genetic disease or abnormality)
10. Is it possible that the pedigree above is for an X-linked recessive trait?
11. Write the genotype next to the symbol for each person in the pedigree
12. Is it possible that the pedigree above is
for an X-linked recessive trait?
43
Clues
Autosomal Recessive
Affected
AA
Aa
aa
X- chromosome linked
recessive
X X
X Y
Autosomal Dominant
Unaffected
aa
AA
Aa
XX
XX
XY
TERMINOLOGIES
Allele = A factor or letter that makes up a gene. 2 alleles make up one gene. Alternative forms of a genetic
locus; a single allele for each locus is inherited separately from each parent (e.g., at a locus for eye color
the allele might result in blue or brown eyes).
Alleles = "B" and "b" are different alleles.
Autosomal = refers to genes that are not found on the sex chromosomes. Autosomal chromosomes are
ones that are not XX and XY. A chromosome not involved in sex determination. The diploid human
genome consists of 46 chromosomes, 22 pairs of autosomes, and 1 pair of sex chromosomes (the X and Y
chromosomes).
Carrier = a person who has a defective gene and a Dominant normal gene and therefore, is normal. (Nn)
Centimorgan (cM): A unit of measure of recombination frequency. One centimorgan is equal to a 1%
chance that a marker at one genetic locus will be separated from a marker at a second locus due to
crossing over in a single generation. In human beings, 1 centimorgan is equivalent, on average, to 1 million
base pairs
Chromosomes = 46 are found in human cells. Genes are carried among chromosomes.
Clones: A group of cells derived from a single ancestor.
Cystic Fibrosis = Autosomal recessive. Mucous in lungs... Death in the 20s.
Dominance = This is one of Johann Gregor Mendels principles. In his studies with pea plants Mendel
notices that pure tall plants bred to pure short plants resulted in tall hybrid plants. Tallness was dominant
over shortness.
Dominant = an allele that overpowers another is dominant.
Down's Syndrome = due to an extra chromosome. (21st pair).
Gamete = sperm or egg. Germ Cell. In humans, germ cell contains 23 chromosomes.
Genetics: The study of the patterns of inheritance of specific traits
Gene = Every trait is controlled by a gene. A human has 20,000 genes. Genes are controlled by 2 factors
called alleles. Each allele comes from a parent.
Genotype = All the genes of a beastie equal the genotype of the beastie. (Genes an organism possesses)
Genome: All the genetic material in the chromosomes of a particular organism; size generally given as its
total number of base pairs.
Germ Cell- An egg or sperm cell. A gamete. In humans, a germ cell contains 23 chromosomes.
44
Haploid= A single set of chromosomes (half the full set of genetic material), present in the egg and sperm
cells of animals and in the egg and pollen cells of plants. Human beings have 23 chromosomes in their
reproductive cells.
Hemophilia = sex-linked recessive. Males get it most often.
Heterozygous = means alleles of a gene are "different".
Heterozygosity=The presence of different alleles at one or more loci on homologous chromosomes.
Homozygous = alleles of a gene are "the same"
Homologous chromosomes: A pair of chromosomes containing the same linear gene sequences, each
derived from one parent
Huntington's Chorea = Autosomal Dominant. People die at 40 +... Jerky muscular motions
Hybrid = alleles of a gene are "different" (Hh) See heterozygous.
Independent Assortment: This is Johann Gregor Mendels 2nd principle. States that alleles of one gene
separate independently from alleles of another gene. In other words, eye color does not affect a persons
ability to roll his or her tongue.
In vitro: Outside a living organism.
Karyotype: Photomicrograph of an individuals chromosomes arranged in a standard format showing the
number, size, and shape of each chromosome type;
Linkage: Proximity of two or more genes on a chromosome; the closer together the genes, the lower the
probability that they will be separated during meiosis and hence the greater the probability that they will be
inherited together.
Linkage map: A map of the relative positions of genetic loci on a chromosome, determined on the basis of
how often the loci are inherited together. Distance is measured in centimorgans (cM).
Locus (pl. loci): The position on a chromosome of a gene or other chromosome marker; also, the DNA at
that position. The use of locus is sometimes restricted to mean regions of DNA that are expressed.
Meiosis = the kind of cell division that produces sperm and egg. Meiosis cuts the number of chromosomes
in half. In humans, for instance, the nuclei of body cells contain 46 chromosomes. Due to meiosis, sex cells
carry only 23 chromosomes one chromosome from each original homologous pair.
Mendel, Johann Gregor = The father of genetics (said that traits are controlled by 2 factors etc...)
Mutation = Change in the DNA instructions. Change in DNA sequence. Change can be beneficial,
detrimental or neutral. Ultimately results in change in protein. For instance, random genetic mutation gave
rise to the dark phenotype of the peppered moth.
Non-Disjunction: When homologous chromosomes fail to segregate properly during meiosis. Down
syndrome, Turner syndrome and Klinefelter syndrome result from non-disjunction.
Phenotype = the way an organism looks.( EXTERNAL CHARACTERISTICS)
Recessive = A small, weaker allele is recessive. (CANNOT EXPRESS ITSELF IN HETEROZYGOUS
CONDITION)
Segregation = One of Mendels principles. Mendel said all genes are comprised of 2 factors, one from
each parent. Chromosomes segregate during meiosis. These factors (alleles) of a gene separate during the
formation of gametes (sperm and egg). This ensures that each parent contributes 50% of their genetic
information.
Sex chromosomes = chromosomes that determine sex (XY and XX)
Somatic Cell = Body cell that contains 46 chromosomes in humans.
Tay Sachs = Autosomal recessive. Children die young. Head enlarges....
Trait = is a feature of an organism.
45
1 Mark Questions
Questions
Q1. Mendels work was rediscovered by three scientists independently. Name any two of them.
Q2. How do we predict the frequency of crossing over between any two linked genes ?
Q3. Why did Mendel select pea plant for his experiment?
Q4. In a monohybrid cross the genotypic and phenotypic ratio is 1:2:1. What type of Inheritance is it
example of? Give one example
Q5. If a human zygote has XXY sex chromosomes along with 22 pairs of autosomes. What sex will the
individual be? Name the syndrome
Q6. Which of the following is a dominant & recessive trait in garden peaTall stem, constricted pod.
2 Mark Questions
Q7. A mother with blood group B has a fetus with blood group A father is A. Explain the situations?
Q8. The genes for hemophilia are located on sex chromosome of humans. It is normally impossible for a
hemophilic father to pass the gene to his son. Why?
Q9. Justify the situation that in human beings sex of the child is determined by father and not by mother?
Q10. What is trisomy? Give one example.
3 Marks Questions
Q11. A man with AB blood group marries a woman with AB blood group.
(i)
Work out all possible genotypes & phenotypes of the progeny.
(ii)
Discuss the kind of domination in the Parents & progeny.
Domination in Father Co dominance
Domination in progeny - Dominance
Q12. Enumerate points to establish parallelism between chromosomes & genes.
Ans12. Refer Pg 82 NCERT Book
Q13. What is Pedigree Analysis ? What are the symbols generally used in it?
Ans13. Refer Pg87,88 of NCERT Book
(3)
(3)
5 Marks Questions
Q14. A dihybrid heterozygous round, yellow seeded garden pea was crossed with a double recessive
plant.
(i)
What type of cross is this ?
(ii)
Work out the genotype & phenotype of the progeny.
(iii)
What principle of Mendel is illustrated by it ?
Ans14. Test Cross
(1)
Working out
(3)
Principle of segregation
(1)
Q15. Describe the nature of inheritance of the ABO blood group in humans. In which ways does this
inheritance differ from that of height of the plant in garden pea?
Ans15. Refer Pg 77 NCERT Book
(3)
Dominance & multiple allelism where as height shows dominance
(2)
46
Chapter 6. MOLECULAR BASIS OF INHERITANCE
DNA largest macromolecule made of helically twisted, two, antiparallel

polydeoxyribonucleotide chains held together by hydrogen bonds.
X-ray diffraction pattern of DNA by Rosalind Franklin showed DNA a helix.
Components of DNA are (i) deoxyribose sugar, (ii) a phosphate, and (iii) nitrogen containing organic
bases.
DNA contains four different bases called adenine (A), guanine (G) cytosine (C), and thymine (T).
These are grouped into two classes on the basis of their chemical structure: (i) Purines (with a double
ring structure) and (ii) Pyrimidines (with a single ring structure)
1953.James Watson and Francis Crick proposed three dimensional structure of DNA
DNA double helix with sugar phosphate back bone on outside and paired bases inside.
Planes of the bases perpendicular to helix axis.
Each turn has ten base pairs.( 34 A0)
Diameter of helix 20 A0.
Two strands of DNA antiparallel.
DNA found both in nucleus and cytoplasm.
Extranuclear DNA found in mitochondria and chloroplasts.
Two chains complementary
Two chains held together by hydrogen bond.
Adenine-Thymine pair has two hydrogen bonds.
Guanine-Cytosine pair has three hydrogen bonds.
Upon heating at temperature above 80-90 degree two strands uncoil and separate (Denaturation)
On cooling two strands join together (renaturation /annealing)
DNA is mostly right handed and B form.
Bacterial nucleoid consists of a single circular DNA molecule .
PACKAGING OF DNA HELIX

# DNA of eukaryotes is wrapped around positively charged histone proteins to form nucleosome.
# Nucleosome contains 200 base pairs of DNA helix.
# Histone octamer =2(H2a+H2b+H3+H4)
# Linker DNA bears H1 protein
# Chromatin fibres formed by repeated units of nucleosomes.
# Non histone proteins required for packaging.
# Regions of chromatin, loosely packed and stains lightly called euchromatin.
# Regions of chromatin, densely packed and stains darkly is called heterochromatin.
47
DNA AS THE GENETIC MATERIAL

Transformation experiment or Griffith effect.
Griffith performed his experiments on Mice using Diplococcus pneumoniae.
Two strains of bacteria are S-type and R-type cells.
Experiments
Living S-strain Injected into mice Mice killed
Living R-strain Injected into mice Mice lived
Heat Killed S-strain Injected into mice Mice lived
Living R-strain + Heat Killed S-strain Injected into mice Mice killed
# Grffith concluded that R type bacteria is transformed into virulent form.
# Transformation is the change in the genetic constitution of an organism by picking up genes present in
the remains of its relatives.
BIOCHEMICAL CHARACTERISATION OF TRANSFORMING PRINCIPLE
# Proved by Oswarld Avery, Colin Macleod, Maclyn Mc Carty
From this we conclude that DNA is the genetic material.
48
Semi conservative nature of DNA Mathew Messelson and Franklin start.

E.coli
Grown on 15 NH4Cl culture

medium
Both strands of DNA have 15N

(N15 N 15)
Shifted to 14NH4Cl culture

medium
After
DNA extracted subjected to

CSCl density gradient
centrifugations
20 min.
After
40 min.
Hybrid/ Intermediate type

of DNA (N15 N14)
Equal amount of light DNA
(N15 N14) and hybrid DNA
(N15 N14)
8. 3 Replication of DNA In Eukaryotes Definition: "Process by which DNA produces daughter DNA molecules
which are exact copies of the original DNA." In eukaryotes, DNA is double stranded. The two strands are
complementary to each other because of their base sequences. Semi-conservative method of DNA replication
Important points:
(i) Most common method of DNA replication.
(ii) Takes place in the nucleus where the DNA is present in the chromosomes.
(iii) Replication takes place in the S-phase (synthesis phase) of the interphase nucleus.
(iv) Deoxyribose nucleotides needed for formation of new DNA strands are present in nucleoplasm. At the time of
replication, the two strands of DNA first separate. Each strand then acts as a template for the formation of a new
strand. A new strand is constructed on each old strand, and two exactly identical double stranded DNA molecules are
formed. In each new DNA molecule, one strand is old (original) while the other is newly formed. Hence, Watson
and Crick described this method as semi-conservative replication. (A) An overall process of DNA replication
showing replication fork and formation of new strands template and lagging template.
49
The various steps involved in this process are summarized as follows:

i. Mechanism of replication starts at a specific point of the DNA molecule, called origin.
ii. At origin, DNA strand breaks because of an incision (nick). This is made by an enzyme called incision enzyme
(endonuclease).
iii. The hydrogen bonds joining the two strands are broken by the enzyme.
iv. The two strands start unwinding. This takes place with the help of a DNA unwinding enzyme Helicases. Two
polynucleotide strands are thus separated.
v. The point where the two strands separate appears like a fork or a Y-shape. This is described as a replicating
fork.
vi. A new strand is constructed on each old strand. This takes place with the help of a small RNA primer molecule
which is complimentary to the DNA at that point.
vii. Each old DNA strand acts as a template (site) for the construction of new strand. The RNA primer attaches itself
to the old strand and attracts the enzymes(DNA polymerase III) which add new nucleotides through base
complementation. The deoxyribose nucleotides are present in the surrounding nucleoplasm. New DNA strand is
thus constructed opposite to each old strand
viii. Formation of new complementary strand always begins at the 3' end of the template strand (original strand) and
progresses towards the 5' end (i.e in 3' - 5' direction). Since the new strand is antiparallel to the template strand, it is
obvious that the new strand itself is always developed in the, 5'-3' direction. For this reason when the two original
strands separate (then with respect to the origin of separation), one acts as 3'-5' template while the other acts as 5'3' template.
ix. Of the two, the replication of 3'-5' template begins first. Hence the new strand formed on it is called the leading
strand. The other template (5'-3') must begin replication at the fork and progress back toward the previously
transcribed fragment. The new strand formed on it is called the lagging strand.
x. Replication of the lagging strand takes place in small fragments called Okazaki fragments. These are then
connected together by the enzyme ligase.
xi. Replication may take place in only one direction on the DNA helix (unidirectional) or in two directions
(bidirectional).
xii. At the end of the process, two double stranded DNA molecules are formed from the original DNA molecule.
Transcription in Prokaryotes
Promoter+
RNA polymerase
With Sigma factor
Co ding Strand
Template Strand
Ribonucleotide
With the help of rho factor
Terminator
RNA
50
Transcription in Eukaryotes
RNA Polymerase
DNA template strand
Primary transcripts (Exon + Intron)
Splicing
Introns removed
Exons joined (hn RNA)

Tailing at 3 end (200-300
Adenylate residue)
Capping at 5 end
(Methylated Guanine)
m RNA (Released from nucleus to

cytoplasm)
GENETIC CODE
Initiation
Codon AUG
commaless
Triplet
Universal
Non ambigious
Genetic Code
Degenerate
Non
overlapping
NonambiguousParticular codon will always code for same amino acid.

DegenerateNumber of codons can code for one amino acid.
UniversalSpecific codon codes for same amino acid in all organisms.
51
Linear
Nonsense codon
(UAA,UAG,UGA)
Translation: Process of joining of amino acids by peptide bond to form a polypeptide.
1. Activation of amino acids

AA+ATP+E Mg+2 AA-AMP-E+ PPi
AA-AMP-E+tRNA AA-tRNA+AMP+E
2. Initiation
Small subunit (40s) of ribosome binds with mRNA.
Charged t RNA specific for initiation codon reaches P site
Larger subunit (60s) of ribosome now combines with 40s-m RNAt
RNA met complex in the presence of Mg+2
3. Elongation
Second t-RNA charged with amino acid occupies A site of ribosome.
Peptide bond formation between methionine and second amino
acids with the help of enzyme peptide transferase.
Ribosomes moves over m RNA in 53
4. Terminator
Translation stops when non sense codons (Stop codons) reached.
No t RNA for stop codons (UAA,UAG,UGA)
Synthesized polypeptide is released with the help of release factor.
* AAAmino acid
*ATPAdenosine Triphosphate
*EPyrophosphate
AAAMP-E-Amino acid adenylate enzyme complex
AAt RNAAmino acyl-t RNA complex
LAC OPERON
*Discovered by Jacob and Manod.
*Experimented on E.coli.
Refer to figure number 6.14 of page 117 of text Book
52
SWITCH OFF CONDITION

i-gene
Repressor Protein + Operator gene
RNA polymerase can not access the structural

gene due to repressor operator complex (ROC)
No transcription of the structural gene
No enzyme or protein formation
SWITCH ON CONDITION
i-gene
Repressor Protein+ Inducer (Lactose)
Repressor Inducer complex (RIC)
Structural gene accessed by RNA

polymerase (no blockage at operator)
Transcription of Structural gene
Enzyme/ protein formed
53
Methodologies of Human Genome Project
Sequence annotation (Sequence the

whole set of genome)
Expressed sequence Tags (EST)

(Identifying all the genes Expressed as
RNA)
-Isolation of total DNA from the

cell
-Fragmentation by restriction
endonuclease
Fragments cloned in suitable host

BAC/YAC
Fragments sequenced using

automated DNA sequences.
Sequences arranged on the basis

of overlapping regions.
Alignment of the sequences by

specialized computer based
programmes
54
Functions of 50%
discoursed genes
unknown
Repetitive sequences
contribute large portion
Largest gene
dystrophic
Average gene consists

of 3000 bases
3164.7 millions
Nucleotides
Total genes 30,000
Salient features
of Human
Genome
Y chromosome has 231 genes
<2% gene codes protein
Chromosome-1 has
2968 gene
Application of Human genome project
-: Identification of defective genes.

-: Opportunity to offer early treatment.
-: Identification of genes that confer susceptibility to certain disease.
-: Prediction of protein that the genes produce.
-: Drug designing to enhance or inhibit the activities of the proteins.
TECHNIQUE FOR DNA FINGER PRINTING

Technique developed by Dr.Alec Jeffreys.
Process is also known as DNA typing/DNA profiling.
DNA extraction from the cells in high speed refrigerated centrifuge
Amplification of DNA content by PCR (Polymerase chain reactions)
DNA fragmentation by Restriction endonuclease
Gel electrophoresis
Double stranded DNA split into single stranded
Southern blotting (Transferring separated DNA to nylon or nitrocellulose sheet)
Nylon sheet immerse in a bath having probes/marker* (Hybridisation)
Nylon membrane pressed on X-ray film (Autoradiography)
Dark band develops at probe site
*Probes/ Markers are radioactive synthetic DNA complementary to VNTR
55
QUESTIONS
ONE MARK QUESTION
1.Name the genetic material in TMV.
2.Write the scientific name of the plant on which Taylor et al performed their experiment.
3.What would be the proportion of light and hybrid density DNA molecules after 80 minutes
of a single
cell of E. coli growth?

4.When does DNA replicate in the cell cycle ?
5.Name the amino acids having only one codon.
TWO MARK QUESTION
1.What is meant by semiconservative nature of DNA replication?
2. What are the functions of DNA polymerase?
3. What is frame shift mutation ?Name the type of mutation that does not affect protein synthesis .
4.What are the untranslated regions (UTRs) ?
5.Briefly describe polymorphism.
THREE MARK QUESTIONS

1.Describe the discontinuous synthesis of DNA.
2. How is Lac operon switched on in an E.coli cell ?
3.Name the three RNA Polymerases found in eukaryotes and mention their functions.
4.Explain the two major approaches involved in the sequencing of genomes.
FIVE MARKS QUESTIONS

1.Describe the salient features of the double helical model of DNA.
2. Bring out the salient features of genetic code .
3.Describe in detail the steps in the technique of DNA finger printing.
4.Describe the process of replication of DNA.
5. What is satellite DNA ?Name their types. Mention their basis for the classification of satellite DNA.
56
CHAPTER-7: EVOLUTION
Evolution: Process that results in heritable changes in a population spread over many generations
(change in allele frequencies over time) leading to diversity of organisms on earth. It is the genetic change
in a population or species over generations( Genes mutate, individuals are selected, and populations
evolve).
Evidences of evolution:
From comparative anatomy: Comparison of body structures amongst different species comes under
comparative anatomy. Certain anatomical similarities among species bear witness to evolutionary history.
e.g. the same skeletal elements make up the forelimbs of man, horse, whale and bat, but each of them
performing different function. However, structural similarities in all mammals descended from a common
ancestory with prototype forelimbs are common suggesting homology. Comparative anatomy confirms
that evolution is a remodeling process. Ancestral structures that originally functioned in one capacity
become modified as they take on new functions-descent with modification.
Vestigial organs: functionless homologous organs that have no apparent function in certain organism.
(supposed to be remnants of organs that had been well developed and functional in their ancestral state
but had become modified during evolution)
E.g. 1. Vermiform appendix in man,2. Pelvic girdle in python,3. Nictitating membrane,4. Coccyx or tail
vertebrae in man.
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Divergent Evolution: Evolutionary pattern in which two species gradually become increasingly different.
This type of evolution often occurs when closely related species diversify to new habitats. On a large scale,
divergent evolution is responsible for the creation of the current diversity of life on earth from the first living
cells. On a smaller scale, it is responsible for the evolution of humans and apes from a common primate
ancestor. Adaptive radiation is one example of divergent evolution.
Adaptive radiation: Diversification, over evolutionary time, of a species or group of species into
several different species or subspecies that are typically adapted to different ecological Group of organisms
diversify greatly and take on new ecological roles. (for example, Darwin's finches in the Galapagos
Island and Marsupials in Australia).
Convergent Evolution :Convergent evolution takes place when species of different ancestry begin to
share analogous traits because of a shared environment or other selection pressure. For example, whales
and fish have some similar characteristics since both had to evolve methods of moving through the same
medium: water.
Parallel Evolution :Parallel evolution occurs when two species evolve independently of each other,
maintaining the same level of similarity. Parallel evolution usually occurs between unrelated species that do
not occupy the same or similar niches in a given habitat.
Biological Evolution:
In the early 1800s French naturalist Jean Baptiste Lamarck suggested that evolution is a process of
adaptation , the refinement of charecteristics that equip organisms to perform successfully in their
environment.However, unfortunately we remember Lamarck for his erroneous view of how adaptation
evolve (the inheritance of acquired characters).
Branching descent and natural selection are the two key concepts of Darwinian Theory of evolution.
According to him all the species inhabiting earth today descended from ancestral species (descent with
modification)and natural selection is the mechanism for such descent with modification. Natural Selection
states that a population of organisms can change over the generations if individuals having certain heritable
traits leave more offspring than other individuals, resulting in a change in the populations genetic
composition over time.
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Stabilizing selection favors the norm, the common, average traits in a population .In nature, natural
selection is most commonly stabilizing. The average members of the population, with intermediate body
sizes, have higher fitness than the extremes. Stabilizing selection culls extreme variants from the
populations.
Directional selection shifts the overall makeup of the population by favoring variants of one extreme
within a population. Natural selection may be directional: it may favor, for example, smaller individuals and
will, if the character is inherited, produce a decrease in average body size. Directional selection could, of
course, also produce an evolutionary increase in body size if larger individuals had higher fitness.
Disruptive selection, like directional selection, favors the variants of opposite extremes over intermediate
individuals. Disruptive selection differs in that sudden changes in the environment creates a sudden force
favoring that In nature, sexual dimorphism is probably a common example.
Founder Effect: A cause of genetic drift attributable to colonization by a limited number of individuals from
a parent population. When few individuals colonize a new habitat, genetic drift will more than likely occur.
The founder population is small and again the alleles present in this small population will not be
representative of the original population. Saltation (from Latin, saltus, "leap") is a sudden change from one
generation to the next, that is large, or very large, in comparison with the usual variation of an organism.
The term is used for occasionally hypothesized, nongradual changes (especially single-step speciation)
that are atypical of, or violate, standard concepts involved in neo-Darwinian evolution.
Natural Selection: states that a population of organisms can change over the generations if individuals
having certain heritable traits leave more offspring than other individuals,resulting in a change in the
populations genetic composition over time.
Artificial selection: Process by which humans breed animals and cultivate crops to ensure that future
generations have specific desirable characteristics.(In artificial selection, breeders select the most desirable
variants in a plant or animal population and selectively breed them with other desirable individuals).
Big bang theory: States that the universe began in a state of compression to infinite density, and that in
one instant all matter and energy began expanding and have continued expanding ever since.
Genetic drift: Changes in the frequencies of alleles in a population that occur by chance, rather than
because of natural selection.
Gene flow: movement of genes into or through a population by interbreeding or by migration.
Gene frequency: The frequency in the population of a particular gene relative to other genes at its locus.
Expressed as a proportion (between 0 and 1) or percentage (between 0 and 100 percent).
Gene pool: All the genes in a population at a particular time.
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Hardy-Weinberg principle: In population genetics, the idea that if a population experienced no

selection, no mutation, no migration, no genetic drift, and are random mating,then the frequency of
each allele and the frequencies of genotype in the population would remain the same (constant)
from one generation to the next generation.
p2 + 2pq + q2 = 1 or, (p + q)2 = 1
Calculation of allele frequencies
Recessive traits: If the frequency of a recessive trait such as cystic fibrosis or PKU is known, it is
possible to calculate allele frequencies and genotype frequencies using the Hardy Weinberg equation and
its assumptions are as follows:
i. say 1 in 1, 2500 Indian newborns have cystic fibrosis which means that the frequency of
homozygotes for this recessive trait is
q = 1/2,500 = 0.0004
ii. The square root of the frequency of recessives is equal to the allele frequency of the cystic
fibrosis allele
q = (0.0004)0.5 = 0.02
iii. The frequency of the normal allele is equal to 1 - the frequency of the cystic fibrosis allele
p = 1- q = 1 - 0.02 = 0.98105
iv. The frequency of carriers (heterozygotes) for the cystic fibrosis allele is
2pq = 2 (0.98)(0.02) = 0.04 or 1/25
v. The frequency of homozygotes for the normal allele is
p = (0.98) = 0.96vi.
Thus the population is composed of three genotypes at the calculated frequencies of homozygous normal =
0.96, heterozygous carriers = 0.04, homozygous affected = 0.0004
Time period
10-15 Mya
Name
Dryopithecus (ape like)
Brain capacity
Ramapithecus (man like)

2 mya
1.2 mya
Australopithecines ( cave dwellers)

Homo habilis
Homo erectus
500cc
700cc
800cc to 1300cc
100,000-40,000 Neanderthal man

mya
25000mya
Homo sapiens
1450cc
1650cc
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Remarks
East Africa, Asia; closely related
to chimpanzee
Shivalik Hills; erect posture,
small canine
African Ape Man ; height 1.5mts
Tool Maker, Community Life
Knew how to use fire, larger
teeth
East and central Asia
Modern man ; height1.5 to 1.8
mts; flat face
Synopsis of Human evolution Major Events during Geological Periods(Time scale):

PERIOD
Precambrian:
Cambrian
Ordovician
Silurian
Devonian
Carboniferous
Permian
Triassic
Jurassic
Cretaceous
Tertiary
Quarternary
EVENTS
Origin of life, Oxygen evolution through photosynthesis
Flourishing of the invertebrates, increase in algal diversity,
appearance of vertebrates.
Plants begin to colonize land.
Increase in diversity of fish.
Amphibians appear
Extensive forest,dominance of amphibians,increase in diversity
of insects,first reptiles appear.
Age of reptiles begin
Dinosaurs evolve and spread, first mammal appear
First bird and first flowering plant appear.
Dominance of flowering plants.
Age of mammals begin
Evolution of human, Large mammals and birds become extinct.
Organic Evolution Study Questions

1. Biological evolution is the cumulative changes that occur in a
____________________ over time.
2. The principle source of change (genetic variation) is due to this type of
chromosomal event. _____________________
3. Charles Darwin published his landmark book entitled
________________________________________ in 1859.
4. In his book, Darwin states that the origin of all life forms is due to random
._______________
5. Darwin premise that all humans, animals, and bacteria share a common distant
ancestor is explained with the concept of __________________ with
._________________________
6. Similarities of the structures of between dissimilar species (ex: arm bones) are
called ________________ structures.
7. Darwinian Theory tells us that
_________________ + ________________ = new species
8. Neo-Darwinian Theory tells us that beneficial genetic mutations concentrated in a
population over time can result in the formation of new ____________________.
9. List three characteristics that always provide a selective advantage.
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Evolution Study Questions KEY

1. population.
2. Mutation
3. The Origin of Species
4. chance.
5. descent modification.
6. homologous.
7. mutability + natural selection = new species
8. species.
9. i. self defense ii. reproductive success iii. food gathering ability
Probable questions:
Short Answer Questions
1. Define evolution.
2. Explain the origin of the earth and atmosphere.
3 . Who conducted simulation experiments? What is the significance of this experiment? Explain the
simulation experiment conducted to explain the origin of complex organic molecules from simple molecules.
Long Answer Questions
1. What are homologous organs? What is homology? What do the homologous organs explain as an
evidence of organic evolution?
2. What are analogous organs? What is analogy?What do analogous organs explain as an evidence of
organic evolution?
3. What are vestigial organs? How do they support the organic evolution? Name any four vestigial organs
in human being.
4. Briefly explain the idea of natural selection taking industrial melanism or antibiotic resistance in
bacteria as example.
Ans. Prior to industrialization, the number and frequency of white peppered moth far exceeded that of dark
coloured peppered moth in Liverpool , England since the white moth got selective advantage over dark
variety to avoid predation by concealing in the lichen infested grey tree trunk. However, after the
industrialization, due to disappearance of lichen in a polluted ambience the dark peppered moth got
selective advantage over white moth to avoid predation in the black tree trunk and hence got reproductive
success due to directional selection. A reduction in air pollution due to clean air legislation again lead to
reproductive success of the white variety.
( industrial melanism)
5. What is geological time scale? How do you infer the evidence of evolution from it?
6. Discuss the evidences from morphology and comparative anatomy in support of organic evolution.
7. Comparative embryology gives no less a significant evidence in support of evolution than any other
branch of biology. Substantiate.
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8. Fossils are the documentary evidences in support of evolution. Discuss.

Ans Fossil record provides clear evidence for the evolution of species over time. It also documents the
evolution of major new groups of organisms from previously existing organisms.Fossil records allow the
biologist to reconstruct the history of life on earth.
9. Justify the statement" Galapagos islands are the living laboratories of Evolution".
Ans The Galapagos island are home to 13 species of finches which evolved on the Galapagos island in
isolation from other finches.New species of finches evolved from the single species that originally colonize
the island provide unique example of adaptive radiation thus , supporting evolution.
10. What is Hardy-Weinberg equilibrium? Write the Hardy-Weinberg equation.
11. What is genetic drift?
Ans Genetic drift is the effect of chance.
Genetic drift causes random changes in allele frequencies over time. Genetic drift can cause small
populations to lose genetic variation.It can cause the fixation of harmful, neutral or beneficial alleles.
12. Define founder effect.
Ans Founder effect is a genetic bottle neck that results when a small group of individuals from a larger
source population establishes a new population far from the original population.
13. What is gene flow?
Ans. Gene flow is nothing but exchanging alleles between populations.
Gene flow can introduce new alleles into a population, providing new genetic variation on which evolution
can work. Gene flow makes the genetic composition of populations more similar
14.Discuss Darwin's theory of Natural Selection.
Ans. Natural selection is the effect of advantageous allele.
In natural selection(NS) , individuals that possess certain forms of an inherited phenotypic trait tend to
survive better and produce more offspring than do individuals that possess other forms of trait. NS is the
only evolutionary mechanism that consistently favors alleles that improve the reproductive success of the
organism in its environment.
15.Discuss mechanisms of evolution.
Ans. Genetic variation is the raw material of evolution
Individuals within the populations differ in morphological, behavioral and biochemical traits , many of which
are under genetic control. Genetic variation provides the raw material on which evolution can work.
Evolution can be summarized as a three step process
1) Mutations and genetic rearrangements caused by recombination occur at random .
2) These random events then generate inherited differences in the characteristics of individuals in
populations.
3) Finally, mutation , gene flow , genetic drift and natural selection can cause allele frequencies to change
over time.
Of the four mechanisms of evolutionary change, mutation, gene flow and genetic drift are influenced by
chance events, while, natural selection is a random process.
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CHAPTER 8 : HUMAN HEALTH AND DISEASES

IMPORTANT QUESTION
1.How do saliva and tear help to prevent bacterial infection?
Ans: -saliva and tear contain lysozymes.
-Lysozymes are the enzymes which digest the cell wall of bacteria
-By lysing the cell wall, they kill bacteria and prevent their infection.
2. What is vaccination?How does it help in producing immunity?
Ans:- Vaccination is the process of introducing a preparation of antigenic protein of the pathogens or
weakened or killed pathogen into the body.
-The vaccines include quick multiplication of B and T-lymphocytes;some of them are stored as memory
cells
-The B-lymphocytes quickly produced antibodies,which neutralize the antigen during infection.
3. Write the full form of ELISA.Give an example of the clinical application of ELISA?
Ans:--Enzyme Linked Immune Sorbent Assay.
-ELISA test is used in the diagnosis of AIDS,hepatitis-B and other STDs
4 .What are the advantages of people being healthy ?
Ans-When people are healthy,
a)They are efficient at work which consequently increases productivity and brings economic prosperity
b)Health increases longevity.
c)It reduces infant and maternal mortality
5 .a) Name the respective forms in which the malarial parasite gains entry into
i) Human body and
ii) Body of female Anopheles
b) Name the hosts where the sexual and the asexual reproduction of malarial parasite occur respectively
c) Name the toxin responsible for the appearance of symptoms of malaria in humans.Why do these
symptoms occur periodically ?
Ans-(a) (i)-Sporozoite
(ii)-Gametocyte
(b) -sexual reproduction in mosquito
-asexual reproduction in human body.
(c) Haemozoin
- Haemozoin is released when the RBCs rupture and release the pathogen
-some cells of pathogen enter fresh RBCs and reproduce asexually and repeat the cycle; hence the
symptoms appear periodically .
6. Define innate immunity. Name and explain the category of barrier which involves macrophages.
Ans. Innate immunity refers to all those defence elements with which a person is born and are always
available to protect the body. -Macrophanges form part of the cellular barrier. -The cellular barrier includes
the following specialized cells; (i) Polymorphonuclear leucocytes. (ii) Monocytes. (iii) Natural killer
lymphocytes and (iv) Macrophages. - these cells phagocytose and destroy the invading microbes.
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7. What is meant by writing H2L2 for an antibody? Name any four types of antibodies produced in
our/human body?
Ans. - Each antibody molecule has four peptide chains. - Of them, two are small and called light chains (L)
and two of them are longer and called heavy chains (H); hence written as H2L2. The four types of
antibodies are IgA, IgE, IgG and IgM.
8. How do normal cells get transformed into cancerous neoplastic cells? Mention the differences between
viral oncogenes and cellular oncogenes.
Ans. The transformation of normal cells into cancerous neoplastic cells is induced by physical, chemical
and biological agents collectively called carcinogens; they lose the property of contact inhibition.
Difference:
Viral Oncogenes
Cellular Oncogenes
- These are the genes present in the oncogenic - These are the genes present in normal cells and
viruses, which effect oncogenic transformation of code for growth factors; when activated under certain
the cells they infect.
conditions, can cause oncogenic transformation of the

cell.
9(i) Explain metastasis. Why is it fatal?

(ii) The lymphocytes are of two types B and T-cells. Why are they called so? (iii) A person was injured in a
road accident and required an urgent immune response. What should be done?
Ans. (i) Metastasis is the property of tumor cells, which get separated from a tumor, spread to different sites
in the body through body fluids and produce secondary tumors wherever they are lodged. Since secondary
tumors are formed at several parts of the body, it is difficult to be diagnosed and treated; hence it is fatal.
(ii) Those lymphocytes which undergo maturation in the bone marrow are called B-cells while those which
undergo maturation in the thymus are called T-cells.
(iii) Vaccine against Tetanus.
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Chapter-9 strategies for enhancement in food production

Animal Breeding-objectives:
1.Improved growth rate.
2.Increased production
3. Improve Desirable Qualities.
4.Improved resistance to diseases
5.Improved resistance to adverse environmental conditions
Methods: i).Inbreeding:-Breeding between same breed for 4-6 generations.Eg.- cows, baffaloes, poultry In
breeding depression- continued in breeding reduces fertility even productivity. A single outcross often
helps to overcome inbreeding depression
ii) Outbreeding- breeding between unrelated animals Of two types
1.) Out crossing- mating within the same breed but not having ancestors.
2.) Crossbreeding- superior males of one breed are mated with superior females of another
breed to get better progency.e.g.- cows of inferior breed with superior bull. Hisardale- is a new breed of
sheep developed in Punjab by crossing Bikaneri Eves and Marano Rams.
3) Interspecific hybridization- male and female animals of two different species are mated. E.g.mule is crossbreed of male donkey and female horse.
4) Control breeding- it is done by artificial insemination and multiple ovulation embryo transfer technology
(MOET)
(a) Artificial insemination- semen of superior male is collected and injected unto the reproductive tract of
selected female. The spread of certain diseases can be controlled by this method.
(b) MOET- This is a technique for herd improvement. Hormones(FSH) are given to the cow for inducing
follicular maturation and super ovulation. The cow is either mated with best bull or inseminated .It is done in
cattle, sheep, rabbits etc.
Steps in Plant breeding:1 Collection of variability-Collection and preservation of all different wild varieties, species, relatives of
cultivated species etc. are also called germplasm collection.
2.Evaluation and selection of parents-Germplasm is evaluated to identify plants with desirable traits.
3.Cross hybridization among the selected parents-Two plants having two desired characters are
hybridized to get new hybrid having two desired characters.
4.Selection and testing of superior recombinants-Selection of the plants having desired character
combinations.
5.Testing,release and commercialization of new cultivars-Newly selected lines are evaluated for their
yield, agronomic traits, disease resistance etc. and released into the market.
Green revolution - Crop production.
White revolution - Milk production
Blue revolution - Fish production
Biofortification-Breeding crops with higher levels of proteins, vitamins and minerals e.g.vit C rich bitter
gourd,mustard,tomato; protein rich beans lablab etc.
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SCP (Single cell protein )-Microbes such as bacteria, yeast, algae are treated in various ways and used as
food. Eg-spirulina can be grown in waste water(from potato processing plant)
Tissue culture- cultured with any plant part called explant.
Types
1.Meristem Culture When apical part is taken and cultured.
Uses: a)Rapid clonal multiplication
b)Production of virus free plants
c)Production of transgenic plants
d)Germplasm collection
2. Protoplast culture and somatic hybridization- The plant cell lacking cell wall is protoplast. Fusion of
protoplast is done by Polyethylene glycol. Pomato is somatic hybrid of potato and tomato.
3.Micropropagation-Tissue culture technique used for rapid vegetative propagation of ornamental plants
and fruit trees
4.Somaclone-Plants obtained from single plants by vegetative propagation.
Questions
I MARK
Q1.Name two techniques involved in controlled breeding experiments.
Q2.What is blue and green revolution?
Q3. What is inbreeding depression?
Q4. What is Heterosisor hybrid vigour?
Q5.Name the Indian variety of rice patented by an American company.
Q6.What is Pomato?
Q7 .Name the algae used as protein rich food.
Q8.Expand-MOET and SCP.
Q9.What is quarantine?
Q10.What is cultivar?
2 MARK
Q1. What is Biofortification?
Q2.Which part of the plant is best suited for making virus free plants?
Q3.What is breed? What are the objectives of animal breeding?
Q4.Define out-crossing? Suggest an advantage.
Q5.What is artificial insemination?what is its importance?
Q6. What are the differences between aqua and pisciculture?
Q7. What is animal husbandry?
Q8. What is bird flu?
Q9. Name the most common species of honey bees of India?what are the products from the honey bees?
Q10. What is germplasm?How it is maintained?
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3MARKS QUESTIONS
Q1.What does inbreeding mean? Suggest its advantages. What is the danger of inbreeding?
Q2.Name the methods employed in animal breeding. Which method is the best? Why?
Q3. Explain the procedure of MOET technique in cattle.
Q4. What is interspecific hybridization ?Give one example of crop in which it practiced and mention one
advantage.
Q5. What is cross-breeding ?what advantages does it have? Give example
5 MARKS QUESTIONS
Q1. Explain the points that have to be considered for successful bee- keeping?
Q2. Write the scientific name of sugarcane grown in north and south India respectively. Mention their
characteristic features. Mention the characteristic of the hybrid produced by crossing these two varieties .
Hint :North Saccharumbarberi. South Saccharumofficinarum . High yield , thick stems , higher sugar
content , ability to grow in both North and South India
Q 3. Describe various steps involved in plant breeding.
Hint: Collection of variability, Evaluation and selection of parents, Cross hybridisation among the selected
parents, Selection of testing of superior Recombinants, Testing , release and comercialisation of new
cultivars
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Chapter 10: Microbes in Human Welfare

Microbes are present everywhere.
E.g.
Thermal vents of geyser (Temp. above 1000c)
Deep in soil.
Under snow.
Diverse. Protozoa, Bacteria, Fungi, Virus, Viroids, Prions (Proteinaceous infectious agents)
Useful : Antibiotics.
Harmful: cause diseases.

In Household Products:
Everyday : Lactobacillus (LAB) Lactic acid Bacteria form curd from milk.
Increase Vit . B12
Check disease causing microbes in our stomach.
Fermentation of dough for dosa, idli (CO2 produced)
Making bread Bakers yeast.Saccharomyces cerevisiae.
Toddy made from sap of palm.
Cheese making (eg.Swiss cheesse by Propionibacterium sharmanii, Roquefort cheese by fungi.)

In Industrial Products :
Beverages and antibiotics.
Fermentors : Large vessels for growing microbes.

Fermented Beverages :
Beverages like wine, bear, whisky, Brandy, Rum (Saccharomyces cerevisiae)

Malted cereals and fruit juices used to produce ethanol, wine and beer produced without distillation.
Whisky, brandy, rum produced after distillation.
Antibiotics : (Against life)
Penicillin produced by Alexander Fleming from Penicillium notatum while working with Staphylococci
Earnest Chain and Howard Plorey awarded Nobel Prize in 1945 for establishing Penicillin as an effective
antibiotic.
Uses : Treat diseases like plague, whooping cough, diphtheria, leprosy.

Chemicals: Enymes and other Bioactivities Molecules: Uses:
Aspergillus niger for production of Citric Acid.
A cetobacter aceti for production of Acetic Acid.
Clostridium butylicum for production of Butynic Acid.
Lactobacillus for production of Lactic acid.
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Lipases used in detergents to remove oil strains from Laundry.
Pectinases and Proteases to clarify bottled jucies.
Streptokinase (from Streptococcus) as clot buster in patients with myocardial infraction (heart attack).
Cyclosporin A an immunosuppresant used in organ transplant patients (produced by Trichoderma

polysporum)
Statins produced by yeast Monascu spurpureus used as blood, cholesterol lowering agent.
Microbes in sewage Treatment:
Major component of waste water, human excreta.
Waste water sewage.
Cannot be disposed directly into rivers and streams.
Before disposal sewage treated in sewage treatment plants (STPs)
Treatment done in two stages.
Primary : Physical removal of particles large and small by filtration and sedimentation.
Solids primary sludge.
Supernatant effluent.
Secondary: Primary effluent taken to large aeration tanks.
Agitated mechanically and air pumped into it.
Aerobic microbes form masses with fungal filaments flocs.
Microbes consume organic matter in effluent for growth.
BOD ( Biological oxygen demand) reduced.
Passed into settling tank.
Bacterial flocs sedimented (activated sludge)
Small part of activated sludge used as inoculums in aeration tank.
Major part pumped into large anaerobic sludge digesters.
Anaerobic bacteria digest bacteria and fungi.
Bacteria produce gases such as menthane, hydrogen sulphide and CO2 Biogas.
Secondary effluent released into rivers and streams.
No man made technology available till date.
Untreated sewage if released into rivers causes pollution.
Ministry of environment and Forests iniatiated, Ganga Action Plan and Yamuna Action Plan.
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Biogas plant:
Concrete tank 10- 15 mts deep, & slurry or dung fed.
Floating cover placed above rises as biogas content rises.
Connecting pipe for supply of biogas.
Used for cooking and lighting.
Development by IARI :- Indian Agriculture Research institute & KVIC:-Khadi and village Industries
Commission.
Microbes as BiocontrolAgents :
Insecticides and Pesticides toxic, harmful & are pollutants.
Natural predation better method.
No of pests kept in check, not totally eradicated.
Food chains not disturbed
Eg. Ladybird and Dragon flies useful to get rid of aphids and mosquitoes.
Bacill us huringiensis(Bt) used to control butterfly caterpillar.
Mode of spores operation.

oAvailable is sachets, mixed with water and sprayed on plants.
oEaten by insect larva
oToxin released in gut kills larvae.
71

Now Bt toxin genes introduced into plants resistant to insect pests.
e.g. Bt cotton.
Tungus trichoderma now being developed.
Nucleo polyhedrovirus good for narrow spectrum insecticide applications.

Advantages :
No negative impacts on plants, mammals, birds, fish or target insects.
For overall IMP (Intergrated pest Management) programme.
For ecologically sensitive areas.

As Biofertilizers:
Chemical fertilizers major pollutant.
Switch to organic farming and use of biofertilizers need of the time.
Main sources of biofertilizers. Bacteria, Fungi & Cyanobacteria.

Eg Rhizobium present in roots of leguminious plants fix atmospheric nitrogen into usable organic form.
Azospirillium and Azotobacter free living bacteria fix atmospheric Nitrogen.
Symbiotic Associations
Eg.Genus Glomus sp. form mycorrhiza
Fungal symbiont absorbs phosphorus from soil and passes it to plant.
Plants show
resistance to root borne pathogens.
Tolerance to salinity and drought
Increase in growth and development.
Cynobacteria autotrophic fix atmospheric nitrogen
Imp.biofertilizer.
e.g. Anabaena, Nostoc, Oscillatoria.
Blue green algae increase fertility by adding organic matter.
No. of biofertilizers are commercially available.

Process of sewage treatment in STP
a)Primary treatment(physical )
b)Secondary treatment(biological)
Effluent loaded in large aeration tank, Agitation & rapid growth of aerobic microbes (flocs) ,Consumes
organic matter ,reduces BOD, Effluent passed to settling tank, Flocs sediments form activated
sludge(A.S.),Poured into sludge digester(small amount of A.S. used as inoculum) Filtration &
sedimentation.
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Process of sewage treatment in STP

Primary treatment (physical)
Filtration & sedimentation
Secondary treatment (biological)

Filtration &sedimentation
Agitation & rapid growth of aerobic microbes (flocs)
Consumes organic matter, reduces BOD
Effluent passed to settling tank
Flocs sediments form activated sludge
Anaerobic Sludge Digester
Form Biogas
Water released into rivers and streams
Questions
(1 mark)
1. Name two vitamins produced by microbial fermentation.
2. What is the botanical name of bakers yeast?
3. Milk starts to coagulate when lactic acid bacteria is added to warm milk as a starter. Mention two benefits
LAB provides
(2 marks)
1. State the use of:
Trichoderma with respect to organ transplant
Nucleopolyhedrovirus with respect to pest management

2. Why should sewage be treated before its disposal?
3. What is primary sludge?
4. Name the pests, lady birds and dragonflies help to get the rid of respectively
5. Give the role of microbes in single cell protein.
6. What is micorhiza? How does it help as biofertilizers?
7. What is BOD? What does it mean if a water sample has more BOD?
8. Name any two cyanobacteria. How do they serve as main source of biofertilizer ?
9. What is the difference between Bt and Bt cotton? Explain the use of Bt as a biological control.
10. Give reasona) Bottled fruit juices brought from market are clearer as compared to those made at home,
b) Large holes are found in swiss-cheese,
c) The insect which are so called pest are not eradicated in organic fumes.
11. Name the gobar gas liberated from biogas plant. Which type of bacteria are responsible for its
production? Give advantage.
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(3 marks)
1. Differentiate between
a) Primary sludge and activated sludge,
b) Biofertilizer and chemical fertilizer,
c) Primary sewage treatment and secondary sewage treatement.
(5 marks)
1.Answer briefly:
1) How is sewage harmful to man?
2) What is organic farming?
3) Which group of organisms attack insect and arthopod? How are they best biocontrol biological
agent,
4) What is the difference between flocks and primary sludge?
2.Write short notes on: a) bakers yeast, b) alcohol c) statin d)Brewers yeast e) streptokinase
74
Chapter-11: BIOTECHNOLOGY: PRINCIPLES AND PROCESSES

CONCEPT MAP :-
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Biotechnology is a broad area of science involving multiple disciplines designed to use living organisms or
their products to perform valuable industrial or manufacturing processes or applications pertaining to
human benefit.
Recombinant DNA technology:
An organism's genome contains virtually all the information necessary for its growth and development
Steps in producing recombinant DNA
1. The required gene is cut from a DNA molecule using a restriction enzyme.
2. A bacterial plasmid is isolated and cut with the same restriction enzyme. This ensures cut ends are
complementary (same base sequence) to the ends of the required gene.
3. The required gene is joined to the plasmid using the enzyme DNA ligase in a process called ligation.
4. The resulting recombinant plasmid is returned to the bacterial cell.
5. The bacteria reproduce and the required gene is cloned.
How do we obtain DNA and how do we manipulate DNA?

Quite straightforward to isolate DNA
For instance, to isolate genomic DNA
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1. Remove tissue from organism

2. Homogenise in lysis buffer containing guanidine thiocyanate (denatures proteins)
3. Mix with phenol/chloroform - removes proteins
4. Keep aqueous phase (contains DNA)
5. Add alcohol (ethanol or isopropanol) to precipitate DNA from solution
6. Collect DNA pellet by centrifugation
7. Dry DNA pellet and resuspend in buffer
8. Store at 4C
Each cell (with a few exceptions) carries a copy of the DNA sequences which make up the organism's
genome.
How do we manipulate DNA?
It used to be difficult to isolate enough of a particular DNA sequence to carry out further manipulation
and/or characterisation of its molecular sequence
Recombinant DNA Technology
Techniques for
- Isolation
- Digestion
- Fractionation
- Purification of the TARGET fragment
- Cloning into vectors
- Transformation of host cell and selection
- Replication
- Analysis
- Expression of DNA
DNA is manipulated using various enzymes that modify and/or synthesise it

Until 1970 there were no convenient methods available for cutting DNA into discrete, manageable
fragments.
1970 - The Beginning of the Revolution
Discovery of a restriction enzyme in the bacterium Haemophilus influenzae
Restriction enzymes
. Restriction enzymes are endonucleases
Bacterial enzymes
Different bacterial strains express different restriction enzymes
The names of restriction enzymes are derived from the name of the bacterial strain they
are isolated from
Cut (hydrolyse) DNA into defined and REPRODUCIBLE fragments
Basic tools of gene cloning
Names of restriction endonucleases
Titles of restriction enzymes are derived from the first letter of the genus +
the first two letters of the species of organism from which they were isolated.
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Source microorganism
Enzyme
Arthrobacter luteus
Alu I
Bacillus amyloiquefaciens H
Recognition Site
Ends produced
AG
CT
Blunt
Bam HI
G
GATCC
Sticky
Escherichia coli
Eco RI
G
AATTC
Sticky
Haemophilus gallinarum
Hga I
Haemophilus infulenzae
Hind III
A
AGCTT
Sticky
Providencia stuartii 164
Pst I
CTGCA
G
Sticky
Nocardia otitiscaviaruns
Not I
GC
GGCCGC
Sticky
Staphylococcus aureus 3A
Sau 3A
GATC
Sticky
Serratia marcesans
Sma I
CCC
GGG
Blunt
GACGC(N)5
Thermus aquaticus
Taq I
T
CGA
Restriction enzymes recognise a specific short nucleotide sequence
Sticky
Sticky
This is known as a Restriction Site

The phosphodiester bond is cleaved between specific bases, one on each DNA strand
The product of each reaction is two double stranded DNA fragments

Restriction enzymes do not discriminate between DNA from different organisms
Restriction endonucleases are a natural part of the bacterial defence system
Part of the restriction/modification system found in many bacteria
These enzymes RESTRICT the ability of foreign DNA (such as bacteriophage DNA) to
infect/invade the host bacterial cell by cutting it up (degrading it)
The host DNA is MODIFIED by METHYLATION of the sequences these enzymes
recognise
o Methyl groups are added to C or A nucleotides in order to protect the bacterial
host DNA from degradation by its own enzymes
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Types of restriction enzymes

Type I Recognise specific sequencesbut then track along DNA (~1000-5000 bases)
before cutting one of the strands and releasing a number of nucleotides (~75) where the
cut is made. A second molecule of the endonuclease is required to cut the 2nd strand of
the DNA
o e.g. EcoK.
o Require Mg2+, ATP and SAM (S-adenosyl methionine) cofactors for function
Type II Recognise a specific target sequence in DNA, and then break the DNA (both
strands), within or close to, the recognition site
o e.g. EcoRI
o Usually require Mg2+
Type III Intermediate properties between type I and type II. Break both DNA strands at a
defined distance from a recognition site
o e.g. HgaI
o Require Mg2+ and ATP
Hundreds of restriction enzymes have been isolated and characterised
Enables DNA to be cut into discrete, manageable fragments
Type II enzymes are those used in the vast majority of molecular biology techniques
Many are now commercially available
Many Type II restriction endonucleases recognise PALINDROMIC sequences (From Greek palindromos,
running back again, recurring : palin, again)
A segment of double-stranded DNA in which the nucleotide sequence of one strand reads in reverse order
to that of the complementary strand. (always read from the same direction)
For example, EcoRI recognises the sequence
5'-G A A T T C-3'
3'-C T T A A G-5'
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Different enzymes cut at different positions and can create single stranded ends ('sticky ends')
Some generate 5' overhangs - eg: EcoRI
Some generate 3' overhangs - eg: PstI
Some generate blunt ends- eg: SmaI
Examples of restriction enzymes and the sequences they cleave

The 'sticky' overhangs are known as COHESIVE ENDS
The single stranded termini (or ends) can base pair (ANNEAL) with any complementary
single stranded termini
This is the basis for RECOMBINANT DNA TECHNOLOGY
Inserting foreign DNA into a cloning vector
Restriction enzymes are a useful tool for analysing Recombinant DNA
After ligating a particular DNA sequence into a cloning vector, it is necessary to check that the correct
fragment has been taken up. Sometimes it is also necessary to ensure that the foreign DNA sequence is in
a certain orientation relative to sequences present in the cloning vector.
Checking the size of the insert
Checking the orientation of the insert
Determining pattern of restriction sites within insert DNA
DNA fractionation
Separation of DNA fragments in order to isolate and analyse DNA cut by restriction enzymes
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Electrophoresis
Electrophoresis is a technique used to separate and sometimes purify macromolecules - especially
proteins and nucleic acids - that differ in size, charge or conformation. When charged molecules are placed
in an electric field, they migrate toward either the positive or negative pole according to their charge.
DNA is electrophoresed through the agarose gel from the cathode (negative) to the anode (positive) when
a voltage is applied, due to the net negative charge carried on DNA
When the DNA has been electrophoresed,

The gel is stained in a solution containing the chemical ethidium bromide. This compound binds tightly to
DNA and fluoresces strongly under UV light - allowing the visualisation and detection of the DNA.
Recombinant DNA technology:
Recombinant DNA: Plasmids, cloning
What is DNA cloning?
DNA cloning is the isolation of a fragment or fragments of DNA from an organism and placing in a VECTOR
that replicates independently of chromosomal DNA. The RECOMBINANT DNA is propagated in a host
organism, the resulting CLONES are a set of genetically identical organisms which contain the recombinant
DNA
Three main purposes for cloning DNA
1) DNA sequencing
2) Protein production
3) Engineering animals/plants/proteins
Cloning and Expression Vectors
Isolated DNA is cloned into VECTORS for long term storage, propagation of the DNA and for production of
protein from gene(s) encoded in the DNA
What are cloning vectors?
Cloning vectors are extra-chromosomal 'replicons' of DNA which can be isolated and can replicate
independently of the chromosome. Vectors usually contain a selectable marker - a gene that allows
selection of cells carrying the vector e.g. by conferring resistance to a toxin. DNA of interest can be cloned
into the vector and replicated in host cells, usually one which has been well characterised.
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Commonly used vector systems

Bacterial plasmids
Bacteriophages
Cosmids
Yeast artificial chromosomes (YACs)
Ti plasmid (plants)
Eukaryotic viruses such as baculovirus (insect cells), SV40 virus and retroviruses.
Characteristics of a Cloning Vector
Origin of replication (ORI)
This process marks autonomous replication in vector. ORI is a specific sequence of nucleotide in DNA from
where replication starts. When foreign DNA is linked to this sequence then along with vector replication,
foreign (desirable) DNA also starts replicating within host cell.
Selectable Marker
Charecteristics of Selectable marker:
A gene whose expression allows one to identify cells that have been transforrned or transfected with a
vector containing the marker gene.
A marker gene is used to determine if a piece of DNA has been successfully inserted into the host
organism.. A gene, usually encoding resistance to an antibiotic,.A selectable marker will protect the
organism from a selective agent that would normally kill it or prevent its growth.
Restriction sites
It should have restriction sites, to allow cleavage of specific sequence by specific Restriction
Endonuclease. Restriction sites in E.coli cloning vector pBR322 include HindIII , EcoRI , BamHI , SalI, PvuI,
PstI, ClaI etc.
Refer NCERT text book diagram of pBR322
A Cloning Vector that Works with Plant Cells
Most commonly used plant cloning vector "Ti" plasmid, or tumor-inducing plasmid. Found in cells of the
bacterium known as Agrobacterium tumefaciens, normally lives in soil. Bacterium has ability to infect
plants and cause a crown gall, or tumorous lump, to form at the site of infection.
Ti plasmid - called T DNA - separates from the plasmid and incorporates into the host cell genome. This
aspect of Ti plasmid function has made it useful as a plant cloning vector (natural genetic engineer).
Plasmids are the most commonly used vector system. Several types available for cloning of foreign DNA
in the host organism Escherichia coli. Many E. coli plasmids allow the expression of proteins encoded by
the cloned DNA
Bacteriophage another common vector system used for cloning DNA. These are viruses which 'infect' E.
coli. The M13 bacteriophage is a single-stranded DNA virus which replicates in E. coli in a double-stranded
form that can be manipulated like a plasmid. It can be used to produce single-stranded DNA copies which
are useful for DNA sequencing.
Bacteriophage is another bacteriophage which is commonly used to make DNA libraries. It allows the
cloning of larger fragments of DNA than can be incorporated into plasmids.
Transformation is the process by which plasmids (or other DNA) can be introduced into a cell. For
E. coli transformation with plasmids is quite straightforward, plasmids can be introduced by
electroporation or by incubation in the presence of divalent cations (usually Ca 2+) and a brief heat
shock (42C) which induces the E. coli cells to take up the foreign DNA
1. two antibiotic selection and replica plating
2. color selection: blue/white selection using the lacz gene
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Insertional inactivation Subcloning a DNA fragment into an active gene (usually a marker gene whose function can be easily
detected) will disrupt the function of that gene. This can be detected by looking for colonies that no longer
display that phenotype.
Colour selection
A more common method to determine which transformants contain plasmids with inserts is to use colour
selection. For E. coli, this involves the lac complex and blue/white screening.
Colonies carrying plasmid with no insert will be coloured blue whereas colonies carrying recombinant
plasmid will be white.
For plasmids such as pBR322, which contains two antibiotic resistance genes, cloning an insert into one of
these will disrupt that gene and inactivate the resistance to that antibiotic.
Southern/Northern Blotting Analysis
Analysing complex nucleic acid mixtures (DNA or RNA)
The total cellular DNA of an organism (genome) or the cellular content of RNA are complex mixtures of
different nucleic acid sequences. Restriction digest of a complex genome can generate millions of specific
restriction fragments and there can be several fragments of exactly the same size which will not be
separated from each other by electrophoresis.
Techniques have been devised to identify specific nucleic acids in these complex mixtures
Southern blotting - DNA
Northern blotting - RNA
Southern blotting
Technique devised by Ed Southern in 1975, is a commonly used method for the identification of DNA
fragments that are complementary to a know DNA sequence. Allows a comparison between the genome of
a particular organism and that of an available gene or gene fragment (the probe). It can tell us whether an
organism contains a particular gene(DNA fragment) or not
In Southern blotting,
1 Chromosomal DNA is isolated from the organism of interest, and digested to completion with a restriction
endonuclease enzyme.
2 The restriction fragments are then subjected to electrophoresis on an agarose gel, which separates the
fragments on the basis of size.
3 DNA fragments in the gel are denatured (i.e. separated into single strands) using an alkaline solution.
4 Transfer fragments from the gel onto nitrocellulose filter or nylon membrane.
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Fig 7-32, Lodish et al (4th ed.)

DNA is bound irreversibly to the filter/membrane by baking at high temperature (nitrocellulose) or crosslinking through exposure to UV light (nylon).
Final step is to immerse the membrane in a solution containing the probe - either a DNA (cDNA clone,
genomic fragment, oligonucleotide) or RNA probe can be used. This is DNA hybridisation
The membrane is washed to remove non-specifically bound probe, and is then exposed to X-ray film - a
process called autoradiography. The principle of Southern blotting
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PCR(Polymerase Chain Reaction):

PCR is a technique for the in vitro amplification of a desired sequence of DNA. PCR allows the
generation of a large quantity of DNA product (up to several g) from only a few starting copies. It has been
shown that PCR can be used to generate a detectable quantity of DNA from only one starting target (or
template) molecule.
PCR developed in the mid-1980's, has found multiple applications, such as:
1. Rapid amplification of intact genes or gene fragments
2. Generation of large amounts of DNA for sequencing
3. Generation of probes specific for uncloned genes by selective amplification of a specific
segment of cDNA
4. Analysis of mutations for medical applications
5. Detection of minute amounts of DNA for forensic purposes
6. Amplification of chromosomal regions adjacent to genes of known sequence and many
more
Development of PCR won the Nobel prize for Kary Mullis and co-workers.
PCR principle
PCR reaction is a DNA synthesis reaction that depends on the extension of primers annealed to opposite
strands of a dsDNA template that has been denatured (melted apart) at temperatures near boiling. By
repeating the melting, annealing and extension steps, several copies of the original template DNA can be
generated.
The amount of starting material (target) needed is very small
Not necessary to isolate the desired sequence, because it will be defined by the primers that are used in
the reaction. The primers are oligonucleotides complementary to different regions on the 2 strands of
DNA template (flanking the region to be amplified).
The primer acts as a starting point for DNA synthesis. The oligo is extended from its 3' end by DNA
polymerase.
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Primer design
The stages of a PCR reaction
PCR is a cycle of three steps:
1. DENATURATION - the strands of the DNA are melted apart by heating to 95C
2. ANNEALING - the temperature is reduced to ~ 55C to allow the primers to anneal to the
target DNA
3. POLYMERISATION/EXTENSION - the temperature is changed to the optimum
temperature in order for the DNA polymerase to catalyse extension of the primers, i.e. to
copy the DNA between the primers.
The cycle is repeated over and over again - as many times as needed to produce a detectable amount of
product.
Discovery of a thermostable DNA polymerase
The breakthrough came with the discovery of the thermostable DNA polymerase Taq polymerase,
from the thermophilic bacterium, Thermus aquaticus, which lives in hot springs.
Taq polymerase enzyme can resist high temperatures required to melt the template DNA apart without
denaturation (loss of activity) and works best at high temperatures (72C). This led to improved specificity
& sensitivity. Annealing of primers to sites other than the target sequence is significantly reduced at the
higher temperatures used for Taq polymerase.
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Applications of PCR
1) Cloning a gene encoding a known protein
2) Amplifying 'old DNA'
3) Amplifying cloned DNA from vectors
4) Creating mutations in cloned genes
5) Rapid amplification of cDNA ends - RACE
6) Detecting bacterial or viral infection
* AIDs infection
* Tuberculosis (Mycobacterium tuberculosis)
7) Cancer
Detecting mutations that occur in cancer and monitoring cancer therapy. Determining if a patient is free of
malignant cells
8) Genetic diagnosis
a. Diagnosing inherited disorders
* Cystic fibrosis
* Muscular dystrophy
* Haemophilia A and B
* Sickle cell anaemia
b. Diagnosing cancer - certain cancers are caused by specific and reproducible mutations: e,g.
Retinoblastoma - childhood cancer of the eye. The heritable form (germ line mutation of one of the two
retinoblastoma allelles): mutation is detected in all cells. Spontaneous form: only detected in tumour tissue.
c. Blood group typing
d. Prenatal diagnosis eg determining the sex of foetus for those at risk of X-linked disorders
PCR is one of the most versatile techniques invented, and has so many applications that this list could go
on for quite some time.
Downstream processing
It refers to the recovery and purification of biosynthetic products, particularly pharmaceuticals, from natural
sources such as animal or plant tissue or fermentation broth
Stages in Downstream Processing
A widely recognized heuristic for categorizing downstream processing operations divides them into four
groups which are applied in order to bring a product from its natural state as a component of a tissue, cell
or fermentation broth through progressive improvements in purity and concentration.
Removal of insolubles
Product Isolation
ProductPurification
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Product Polishing
GLOSSARY:
Amplification
An increase in the number of copies of a specific DNA fragment; can be in vivo or in vitro.
See also: cloning, polymerase chain reaction
Annotation
Adding pertinent information such as gene coded for, amino acid sequence, or other commentary to the
database entry of raw sequence of DNA bases.
Antisense
Nucleic acid that has a sequence exactly opposite to an mRNA molecule made by the body; binds to the
mRNA molecule to prevent a protein from being made.
Autoradiography
A technique that uses X-ray film to visualize radioactively labeled molecules or fragments of molecules;
used in analyzing length and number of DNA fragments after they are separated by gel electrophoresis.
Bacterial artificial chromosome (BAC)
A vector used to clone DNA fragments (100 to 300 kb insert size; average, 150 kb) in Escherichia coli cells.
Based on naturally occurring F-factor plasmid found in the bacterium E. coli.
Base sequence
The order of nucleotide bases in a DNA molecule; determines structure of proteins encoded by that DNA.
Bioinformatics
The science of managing and analyzing biological data using advanced computing techniques. Especially
important in analyzing genomic research data.
Biotechnology
A set of biological techniques developed through basic research and now applied to research and product
development. In particular, biotechnology refers to the use by industry of recombinant DNA, cell fusion, and
new bioprocessing techniques.
Cancer
Diseases in which abnormal cells divide and grow unchecked. Cancer can spread from its original site to
other parts of the body and can be fatal.
See also: hereditary cancer, sporadic cancer
Carcinogen
Something which causes cancer to occur by causing changes in a cell's DNA.
See also: mutagen
Carrier
An individual who possesses an unexpressed, recessive trait.
cDNA library
A collection of DNA sequences that code for genes. The sequences are generated in the laboratory from
mRNA sequences.
See also: messenger RNA
Cell
The basic unit of any living organism that carries on the biochemical processes of life.
Chromosome
The self-replicating genetic structure of cells containing the cellular DNA that bears in its nucleotide
sequence the linear array of genes. In prokaryotes, chromosomal DNA is circular, and the entire genome is
carried on one chromosome. Eukaryotic genomes consist of a number of chromosomes whose DNA is
associated with different kinds of proteins.
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Clone
An exact copy made of biological material such as a DNA segment (e.g., a gene or other region), a whole
cell, or complete organism.
Cloning
Using specialized DNA technology to produce multiple, exact copies of a single gene or other segment of
DNA to obtain enough material for further study. Process, used by researchers in the Human Genome
Project, referred to as cloning DNA. Resulting cloned (copied) collections of DNA molecules constitute
clone libraries. Second type of cloning exploits the natural process of cell division to make many copies of
an entire cell. The genetic makeup of these cloned cells, called cell line, is identical to the original cell. Third
type of cloning produces complete, genetically identical animals such as the famous Scottish sheep, Dolly.
Cloning vector
DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA
fragment of appropriate size can be integrated without loss of the vector's capacity for self-replication;
vectors introduce foreign DNA into host cells, where the DNA can be reproduced in large quantities.
Examples are plasmids, cosmids, and yeast artificial chromosomes; vectors are often recombinant
molecules containing DNA sequences from several sources.
Complementary DNA (cDNA)
DNA that is synthesized in the laboratory from a messenger RNA template.
Complementary sequence
Nucleic acid base sequence that can form a double-stranded structure with another DNA fragment by
following base-pairing rules (A pairs with T and C with G). The complementary sequence to GTAC for
example, is CATG.
Cosmid
Artificially constructed cloning vector containing the cos gene of phage lambda. Cosmids can be packaged
in lambda phage particles for infection into E. coli; Permits cloning of larger DNA fragments (up to 45kb)
than can be introduced into bacterial hosts in plasmid vectors.
Crossing over
The breaking during meiosis of one maternal and one paternal chromosome, the exchange of
corresponding sections of DNA, and the rejoining of the chromosomes. This process can result in an
exchange of alleles between chromosomes.
See also: recombination
DNA (deoxyribonucleic acid)
The molecule that encodes genetic information. DNA is a double-stranded molecule held together by weak
bonds between base pairs of nucleotides. The four nucleotides in DNA contain the bases adenine (A),
guanine (G), cytosine (C), and thymine (T). In nature, base pairs form only between A and T and between
G and C; thus the base sequence of each single strand can be deduced from that of its partner.
DNA bank
A service that stores DNA extracted from blood samples or other human tissue.
DNA repair genes
Genes encoding proteins that correct errors in DNA sequencing.
DNA replication
The use of existing DNA as a template for the synthesis of new DNA strands. In humans and other
eukaryotes, replication occurs in the cell nucleus.
DNA sequence
The relative order of base pairs, whether in a DNA fragment, gene, chromosome, or an entire genome.
See also: base sequence analysis
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Double helix
The twisted-ladder shape that two linear strands of DNA assume when complementary nucleotides on
opposing strands bond together.
Electrophoresis
A method of separating large molecules (such as DNA fragments or proteins) from a mixture of similar
molecules. An electric current is passed through a medium containing the mixture, and each kind of
molecule travels through the medium at a different rate, depending on its electrical charge and size.
Agarose and acrylamide gels are the media commonly used for electrophoresis of proteins and nucleic
acids.
Electroporation
A process using high-voltage current to make cell membranes permeable to allow the introduction of new
DNA; commonly used in recombinant DNA technology.
See also: transfection
Embryonic stem (ES) cells
An embryonic cell that can replicate indefinitely, transform into other types of cells, and serve as a
continuous source of new cells.
Endonuclease
See: restriction enzyme
Escherichia coli
Common bacterium that has been studied intensively by geneticists because of its small genome size,
normal lack of pathogenicity, and ease of growth in the laboratory.
Eugenics
Study of improving a species by artificial selection; usually refers to the selective breeding of humans.
Exogenous DNA
DNA originating outside an organism that has been introduced into the organism.
Exon
The protein-coding DNA sequence of a gene.
See also: intron
Exonuclease
An enzyme that cleaves nucleotides sequentially from free ends of a linear nucleic acid substrate.
Expressed sequence tag (EST)
A short strand of DNA that is part of cDNA molecule and can act as identifier of a gene. Used in locating
and mapping genes.
See also: cDNA, sequence tagged site
Fingerprinting
In genetics, the identification of multiple specific alleles on a person's DNA to produce a unique identifier for
that person.
See also: forensics
Fluorescence in situ hybridization (FISH)
A Physical mapping approach that uses fluorescein tags to detect hybridization of probes with metaphase
chromosomes and with the less-condensed somatic interphase chromatin.
Forensics
Use of DNA for identification. Some examples of DNA use are to establish paternity in child support cases;
establish the presence of a suspect at a crime scene, and identify accident victims.
Functional genomics
Study of genes, their resulting proteins, the role played by proteins in the body's biochemical processes.
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Gel electrophoresis
See: electrophoresis
Gene
The fundamental physical and functional unit of heredity. A gene is an ordered sequence of nucleotides
located in a particular position on a particular chromosome that encodes a specific functional product (i.e.,
a protein or RNA molecule)
See also: gene expression
Gene expression
The process by which a gene's coded information is converted into the structures present and operating in
the cell. Expressed genes include those that are transcribed into mRNA and then translated into protein
and those that are transcribed into RNA but not translated into protein (e.g., transfer and ribosomal RNAs).
Gene library
See: genomic library
Gene mapping
Determination of the relative positions of genes on a DNA molecule (chromosome or plasmid) and of the
distance, in linkage units or physical units, between them.
Gene pool
All the variations of genes in a species.
See also: allele, gene, polymorphism
Gene therapy
Experimental procedure aimed at replacing, manipulating, or supplementing nonfunctional or
misfunctioning genes with healthy genes.
See also: gene, inherit, somatic cell gene therapy, germ line gene therapy
Gene transfer
Incorporation of new DNA into an organism's cells, usually by a vector such as a modified virus. Used in
gene therapy.
See also: mutation, gene therapy, vector
Genetic engineering
Altering the genetic material of cells or organisms to enable them to make new substances or perform new
functions.
Genetic engineering technology
See: recombinant DNA technology
Genetic marker
A gene or other identifiable portion of DNA whose inheritance can be followed.
See also: chromosome, DNA, gene, inherit
Genetic material
See: genome
Genetic polymorphism
Difference in DNA sequence among individuals, groups, or populations (e.g., genes for blue eyes versus
brown eyes).
Genetic screening
Testing a group of people to identify individuals at high risk of having or passing on a specific genetic
disorder.
Genetic testing
Analyzing an individual's genetic material to determine predisposition to a particular health condition or to
confirm a diagnosis of genetic disease.
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Genetics
The study of inheritance patterns of specific traits.
Genome
All the genetic material in the chromosomes of a particular organism; its size is generally given as its total
number of base pairs.
Genome project
Research and technology-development effort aimed at mapping and sequencing the genome of human
beings and certain model organisms.
See also: Human Genome Initiative
Genomic library
A collection of clones made from a set of randomly generated overlapping DNA fragments that represent
the entire genome of an organism.
Genotype
The genetic constitution of an organism, as distinguished from its physical appearance (its phenotype).
Human Genome Project (HGP)
Formerly titled Human Genome Initiative.
See also: Human Genome Initiative
In situ hybridization
Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in cloned
bacterial or cultured eukaryotic cells.
In vitro
Studies performed outside a living organism such as in a laboratory.
In vivo
Studies carried out in living organisms.
Independent assortment
During meiosis each of the two copies of a gene is distributed to the germ cells independently of the
distribution of other genes.
See also: linkage
Informatics
See: bioinformatics
Karyotype
Photomicrograph of an individual's chromosomes arranged in standard format showing the number, size,
and shape of each chromosome type; used in low-resolution physical mapping to correlate gross
chromosomal abnormalities with the characteristics of specific diseases.
Knockout
Deactivation of specific genes; used in laboratory organisms to study gene function.
See also: gene, locus, model organisms
Marker
See: genetic marker
Microinjection
A technique for introducing a solution of DNA into a cell using a fine microcapillary pipette.
Mitochondrial DNA
Nitrogenous base
A nitrogen-containing molecule having the chemical properties of a base. DNA contains the nitrogenous
bases adenine (A), guanine (G), cytosine (C), and thymine (T).
See also: DNA
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Northern blot
A gel-based laboratory procedure that locates mRNA sequences on a gel that are complementary to a
piece of DNA used as a probe.
Nucleotide
A subunit of DNA or RNA consisting of a nitrogenous base (adenine, guanine, thymine, or cytosine in DNA;
adenine, guanine, uracil, or cytosine in RNA), a phosphate molecule, and a sugar molecule (deoxyribose in
DNA and ribose in RNA). Thousands of nucleotides are linked to form a DNA or RNA molecule.
See also: DNA, base pair, RNA
Nucleus
The cellular organelle in eukaryotes that contains most of the genetic material.
Phage
A virus for which the natural host is a bacterial cell.
Plasmid
Autonomously replicating extra-chromosomal circular DNA molecules, distinct from the normal bacterial
genome and nonessential for cell survival under nonselective conditions. Some plasmids are capable of
integrating into the host genome. Number of artificially constructed plasmids are used as cloning vectors.
Polymerase chain reaction (PCR)
A method for amplifying a DNA base sequence using heat-stable polymerase and two 20-base primers,
one complementary to the (+) strand at one end of the sequence to be amplified and one complementary to
the (-) strand at the other end. Because the newly synthesized DNA strands can subsequently serve as
additional templates for the same primer sequences, successive rounds of primer annealing, strand
elongation, and dissociation produce rapid and highly specific amplification of the desired sequence. PCR
also can be used to detect the existence of the defined sequence in a DNA sample.
Polymerase, DNA or RNA
Enzyme that catalyzes the synthesis of nucleic acids on preexisting nucleic acid templates, assembling
RNA from ribonucleotides or DNA from deoxyribonucleotides.
Primer
Short preexisting polynucleotide chain to which new deoxyribonucleotides can be added by DNA
polymerase.
Probe
Single-stranded DNA or RNA molecules of specific base sequence, labeled either radioactively or
immunologically. Used to detect the complementary base sequence by hybridization.
Restriction enzyme, endonuclease
Protein that recognizes specific, short nucleotide sequences and cuts DNA at those sites. Bacteria contain
over 400 such enzymes that recognize and cut more than 100 different DNA sequences.
See also: restriction enzyme cutting site
Restriction fragment length polymorphism (RFLP)
Variation between individuals in DNA fragment sizes cut by specific restriction enzymes; polymorphic
sequences that result in RFLPs are used as markers on both physical maps and genetic linkage maps.
RFLPs are usually caused by mutation at a cutting site.
See also: marker, polymorphism
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Restriction-enzyme cutting site

Specific nucleotide sequence of DNA at which a particular restriction enzyme cuts the DNA. Some sites
occur frequently in DNA (e.g., every several hundred base pairs); others much less frequently (rare-cutter;
e.g., every 10,000 base pairs).
Retroviral infection
Presence of retroviral vectors, such as some viruses, which use their recombinant DNA to insert their
genetic material into the chromosomes of the host's cells. The virus is then propogated by the host cell.
Reverse transcriptase
Enzyme used by retroviruses to form a complementary DNA sequence (cDNA) from their RNA. The
resulting DNA is then inserted into the chromosome of the host cell.
Ribonucleotide
See: nucleotide
Ribose
The five-carbon sugar that serves as a component of RNA.
See also: ribonucleic acid, deoxyribose
Ribosomal RNA (rRNA)
A class of RNA found in the ribosomes of cells.
RNA (Ribonucleic acid)
Chemical found in nucleus and cytoplasm of cells. Plays important role in protein synthesis and other
chemical activities of the cell. Structure of RNA similar to that of DNA. There are several classes of RNA
molecules, including messenger RNA, transfer RNA, ribosomal RNA, and other small RNAs, each serving
a different purpose.
Sanger sequencing
A widely used method of determining the order of bases in DNA.
See also: sequencing, shotgun sequencing
Satellite
Chromosomal segment that branches off from the rest of the chromosome but is still connected by a thin
filament or stalk.
Scaffold
In genomic mapping, a series of contigs that are in the right order but not necessarily connected in one
continuous stretch of sequence.
Segregation
The normal biological process whereby the two pieces of a chromosome pair are separated during meiosis
and randomly distributed to the germ cells.
Sequencing
Determination of order of nucleotides (base sequences) in a DNA or RNA molecule or the order of amino
acids in a protein.
The X or Y chromosome in human beings that determines the sex of an individual. Females have two X
chromosomes in diploid cells; males have an X and a Y chromosome. The sex chromosomes comprise the
23rd chromosome pair in a karyotype.
94
Shotgun method
Sequencing method that involves randomly sequenced cloned pieces of the genome, with no
foreknowledge of where the piece originally came from. This can be contrasted with "directed" strategies, in
which pieces of DNA from known chromosomal locations are sequenced. Because there are advantages to
both strategies, researchers use both random (or shotgun) and directed strategies in combination to
sequence the human genome.
Single nucleotide polymorphism (SNP)
DNA sequence variations that occur when a single nucleotide (A, T, C, or G) in the genome sequence is
altered.
Single-gene disorder
Hereditary disorder caused by a mutant allele of a single gene (e.g., Duchenne muscular dystrophy,
retinoblastoma, sickle cell disease).
See also: polygenic disorders
Somatic cell
Any cell in the body except gametes and their precursors.
Southern blotting
Transfer by absorption of DNA fragments separated in electrophoretic gels to membrane filters for
detection of specific base sequences by radio-labeled complementary probes.
Transfer RNA (tRNA)
A class of RNA having structures with triplet nucleotide sequences that are complementary to the triplet
nucleotide coding sequences of mRNA. The role of tRNAs in protein synthesis is to bond with amino acids
and transfer them to the ribosomes, where proteins are assembled according to the genetic code carried by
mRNA.
Transgenic
An experimentally produced organism in which DNA has been artificially introduced and incorporated into
the organism's germ line.
See also: cell, DNA, gene, nucleus, germ line
Transposable element
A class of DNA sequences that can move from one chromosomal site to another.
Trisomy
Possessing three copies of a particular chromosome instead of the normal two copies.
See also: cell, gene, gene expression, chromosome
Virus
Noncellular biological entity that can reproduce only within a host cell. Viruses consist of nucleic acid
covered by protein; some animal viruses are also surrounded by membrane. Inside the infected cell, the
virus uses the synthetic capability of the host to produce progeny virus.
See also: cloning vector
Western blot
A technique used to identify and locate proteins based on their ability to bind to specific antibodies.
See also: DNA, Northern blot, protein, RNA, Southern blotting
Wild type
The form of an organism that occurs most frequently in nature.
Yeast artificial chromosome (YAC)
Constructed from yeast DNA, it is a vector used to clone large DNA fragments.
See also: cloning vector, cosmid
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Outline of molecular biology
Questions
1 Mark Questions
1) What is biotechnology?
2) Define plasmid.
3) What are molecular scissors?
4) What do you mean by recognition sequence?
5) Which enzymes act as molecular glue?
6) What is elution?
7) What are cloning vectors?
8) Name the sequence within a cloning vector from where the replication commences.
9) Mention the bacteria that acts as natural genetic engineer.
10) Name any two processes by which alien DNA is introduced into the host cell.
11) Expand the term PCR.
12) Name the microorganism from which the thermostable DNA polymerase required for PCR is obtained?
13) What is a bioreactor?
14) What are the two main processes involved in downstream processing?
HINTS:
1) Large scale production and marketing of products and processes using living organisms, cells or
enzymes.
2) Autonomously replicating circular , extra-chromosomal bacterial DNA used in gene manipulation.
3) Restriction enzymes.
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4) Restriction endonucleases always cut DNA at a specific point by recognizing a specific sequences of
base pair known as recognition sequence.
5) DNA ligases
6) The ultimate step in the separation and isolation of DNA fragments through gel electrophoresis in which
separated bands of DNAs are cut out from the gel and extracted from the gel piece.
7) Cloning vectors are extra-chromosomal 'replicons' of DNA which can be isolated and can replicate
independently of the chromosome. DNA of interest can be cloned into the vector and replicated in host cells
8) ORI point
9) Agrobacterium tumefaciens
10) Microinjection,biolistics(gene gun)
11) Polymerase Chain Reaction 12) Thermusaquaticus
13) Large scale biotechnological product involves the use of bioreactor.
14) Separation and purification.
2-Marks Questions
1) Enlist the core techniques that pave the way for modern biotechnology.
2) What is gene cloning?
3) Mention the three steps involve in genetically modifying an organism.
4) Why do bacteria possesses restriction enzyme ?
5) Mention one basic difference between restriction endonucleases and exonucleases.
6) What is a palindromic sequence? Give example.
7) What are sticky ends and blind ends ?
8) Mention the role of selectable marker in cloning vector.
9) What is insertional inactivation?
10) How can you make a bacterial cell competent to take up foreign DNA ?
HINTS:
1) ( a) Genetic engineering (b) maintenance of sterile ambience.
2) The process of cloning multiple copies of a gene.
3) (a) identification of DNA with desirable genes
(b) introduction of the identified DNA into the host and
(c) maintenance of introduced DNA in the host and transfer of DNA to its progeny.
4) By restriction enzyme bacteria can attack and destroy the phage DNA in case of viral attack and thereby
prevent viral attack.
5) Exonucleases digest DNA from the flank ( beginning/end) of the DNA strands. Whereas endonucleases
catalyses the hydrolytic cleavage of DNA in the middle.
6) A segment of double-stranded DNA in which the nucleotide sequence of one strand reads same in
reverse order to that of the complementary strand. (always read from the same direction)
7) Double stranded ends of a DNA molecule (without any overhangings) produced by the action of certain
restriction enzymes .-[blunt ends)/ Sticky ends - Double stranded ends] of a DNA molecule (with
overhangings) produced by the action of certain restriction enzymes
8) The selectable marker genes in a cloning vector allow for the selection and identification of bacteria that
have been transformed with a recombinant plasmid compared to nontransformed cells. Some of the most
common selectable markers are genes for ampicillin resistance (ampR) and tetracycline resistance (tetR )
and the lacZ gene used for blue white selection.
9) Insertional inactivation refers to the loss of activity of the selectable marker genes due to the insertion of
foreign DNA within the coding sequence of the marker gene in a transfected bacteria.
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3-Marks Questions:
1)Enlist the major steps in recombinant DNA technology.

2)Mention the steps involved in the separation and isolation of DNA fragments through agarose gel
electrophoresis.
3)Describe in brief the principle of DNA isolation through gel electrophoresis.
4)Highlight the salient features that are required to facilitate cloning into a vector.
5) Enumerate the major steps for isolation of DNA.
6) Draw a neat ,labeled diagram of (a) simple stirred tank bioreactor/ (b) sparged tank bioreactor.
Answers:
1) R-DNA Technology:
Restriction enzyme cuts double stranded DNA at its particular recognition sequence.
The cuts produce DNA fragments with cohesive ends
DNA from a plasmid was also cut by the same restriction enzyme
When two of the above mentioned DNA come together they can join by base pairing.
DNA ligase enzyme used to unite the backbones of the two DNA fragments ,producing R-DNA
2) Agarose gel electrophoresis:
3) DNA When charged molecules are placed in an electric field, they migrate toward either the positive or
negative pole according to their charge. In contrast to proteins, which can have either a net positive or net
negative charge, nucleic acids have a consistent negative charge imparted by their phosphate backbone,
and migrate toward the anode DNA is electrophoresed through the agarose gel from the cathode (negative)
to the anode (positive) when a voltage is applied, due to the net negative charge carried on DNA
4)Salient features of a DNA cloning Vectors:
Size: small enough to be easily separated from the chromosomal DNA of the host bacteria.
Ori site; must have the site for DNA replication that allows the plasmid to replicate separately from the host
cells chromosome.
Multiple Cloning sites :a stretch of DNA with recognition sequence for many different commonb restriction
enzymes.
Selectable marker genes
RNApolymerase promoter sequence

5) Major steps for isolation of DNA: Cell containing DNA is treated with lysozyme/cellulose/chitinase
DNA along with RNA,Protein,lipid are released
Treatment with RNAase,protease to remove RNA and Protein
Appropriate treatment to remove other impurities
Addition of chilled ethanol to get precipitation of purified DNA
6) Consult NCERT Textbook page number 204
5-Marks Questions:
1) What do you mean by PCR? Briefly enumerate the major steps of PCR. Mention the utility of PCR.
Ans: PCR is a cycle of three steps:
DENATURATION - the strands of the DNA are melted apart by heating to 95C
ANNEALING - the temperature is reduced to ~ 55C to allow the primers to anneal to the target DNA
POLYMERISATION/EXTENSION - the temperature is changed to the optimum temperature in order for the
DNA polymerase to catalyse extension of the primers, i.e. to copy the DNA between the primers.
The cycle is repeated over and over again - as many times as needed to produce a detectable amount of
product (DNA)
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Chapter-12 BIOTECHNOLOGY & ITS APPLICATION

Biotechnology is making Genetically modified organisms-microbes, plants, animals for industrial
production of Bio-Pharmaceuticals and other useful products.
Applications
i) Diagnostic & therapeutic
ii) Genetically modified crops
iii) Waste treatment
iv) Energy production
v) Food processing
vi) Bioremediation
Application in agriculture
Genetically modified organisms (GMO)-Plants, bacteria, fungi, animals. whose genes are altered by
manipulation.
Transgenic crops(GMO) -Crops contain or express one or more useful foreign genes.
Advantages -i) More tolerant to stresses (heat, cold, drought).
ii) Pest resistants GM crops, reduce the use of Chemical pesticides. Eg- BT-Cotton
iii) Reduced post harvest losses. Eg- Flavr savr tomato.
iv) Enhance nutritional value of food. Eg- Golden Rice (Vitamin A enriched).
v) Increased efficiency of mineral use.
PEST RESISTANT PLANTS
Bt- cotton -BT stands for Bacillus thuringiensis (Soil Bacteria). Bacterium produces proteins
(Crystal Protein-cry I AC, cry II AB). A crystalliane insecticidal protein that kills the insects. Hence cryGenes have been introduced in plants to produce crystal proteins as Protoxin (inactive toxin), which is
converted to toxins in alkaline medium (i.e. in the gut of insects) and cause death of the insect larva.
Protection of plants against nematodes Nematode, Meloidogyne incognita infects tobacco plants &
reduces yield. Specific genes (DNA) from nematodes introduced into the plants using Agrobacterium
tumifecians (soil bacteria). Genes produce sense and antisense complementary RNA. Act as dsRNA and
initiates RNAi ( RNA interference) and silences the specific mRNA. Complementary RNA neutralizes the
specific RNA of nematodes by a process called RNA Interference and parasite cannot live in transgenic
host.
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In medicine- genetically engineered insulin

Human insulin consists of Polypeptide chains A & B. Insulin secreted as Prohormone, which contains C
peptides, removed during maturation.
In 1983, Eli Lilly, an American company prepared 2 DNA sequences coding for chains A & B.
Genes inserted into the cells and tissues to correct certain hereditary diseases.
Gene therapy
Gene therapy corrects the gene defects in child or embryo. Deficiency of ADA causes SCID due to the
disorder of a gene. It can be cured by bone marrow transplantation. Functional ADA-cDNA is introduced in
lymphocyte and returned to the patient.
Molecular diagnosis -- PCR (Polymesase chain reaction) used for early diagnosis of disorder.
ELISA (Enzyme Linked Immunosorbent Assay) used to detect AIDS.
Transgenic Animals
Animals with manipulated genes or a foreign gene to be expressed are called as transgenic animals. They
are useful1. To know how genes contribute to development of disease.
2. To use proteins for treatment of disease.
3. To verify vaccine and chemical safety.
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Biropiracy -- Some organizations and multinational companies exploit or patents bioresources of other
nations without proper authorization. Indian patent bill is there to prevent such unauthorized exploitation.
GEAC- For validity of GM research and the safety of introducing GM organism
Three mark question
1) What is the main advantage of producing genetically engineered insulin?
Ans- Produces only A&B peptides.
No C-Peptides produced .
No need to remove C-Peptides during maturation.
2) What are the advantages of Molecular diagnosis technique?
Ans- 1) Accurate,
Disease can be detected at very early stage
Can be diagonised even if the number of pathogens is very low.
What are the potential risks ( Three ) of using GM food?
Ans Potential risks- i) Products of transgene - allergic or toxic
ii) Cause damage to natural environment
iii) Weeds also become resistant
iv) Can endanger native species
4)What is hirudin? How do you get it?
Ans- Anti coagulant. obtained from transgenic brassica napus.
5) How does agro bacterium help to increase Tobacco production?
Ans - Introduction of Nematode specific gene.
Production of dsRNA(Sense and anti-Sense)
Silence specific MRNA.
6) Why do farmers face the problems in Agro chemical based farming?
Ans - 1. Too expensive 2. Conventional breeding not able to increase production.
7) Why should farmers in India cultivate GM crops?
Ans - Tolerant to stress, pest resistant, less post harvest losses, increased mineral using efficiency.
Five mark question
1)Explain the steps involved in the production of genetically engineered insulin?
Ans- i) Human insulin consists of 51 amino acids arranged in chains of A and B bearing 21 and 30 a. a
respectively interconnected by disulphide bridges.
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Diagram- Maturation of proinsulin into insulin after removal of c- peptide

ii) Insulin synthesized as prohormone has extra c -peptide which is removed during maturation.
iii) In 1983 , Eli Lilly, American company prepared two DNA sequences similar to A and B chains of
human insulin(humulin).
iv) Chain A and B extracted and combined by creating disulphide bonds.
Keywords of the chapter

Genetically Modified Organism(GMO), Bt cotton, insecticidal proteins, cry genes, pest resistant plants, RNA
interference(RNAi)/RNAsilencing, dsRNA, Genetically engineered insulin, gene therapy, ADA deficiency , c
DNA, Molecular diagnosis, transgenic animals, Bio ethics, Genetic Engineering Approval
Committee(GEAC), Bio piracy, Indian patent bill.
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Chapter 13 ORGANISMS AND POPULATIONS

Ecology
# It deals with the interaction (i) Among organisms
(ii) Between organisms (iii) Physical
environment.
Atmosphere
Organism
Organism
Hydrosphere
Lithosphere
Biome
# Combination of various communities.
# Seasonal variation and annual variation lead to biome formation
# E.g. Artic and Alpine tundra, coniferous forest, temperate forest, grass land and desert.
Environment
Abiotic factors
Temperature, soil,
water, light
Biotic factors
Microorganisms, plants,
Animals
Temperature
# Average temperature varies seasonally
# Organisms Eurythermal or Stenothermal
# Organisms affected by Global Warming.
Water
# Influences life of organisms. No life without water.
# Productivity and distribution of plants water dependent.
#Organisms Euryhaline or Stenohaline.
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Light
# Photosynthesis and release of oxygen light dependent.
# Sciophytes need to use diurnal and seasonal light intensity of forage, migration and
reproduction.
Soil
# Nature and proportion of soil in a place depends on climate, weathering process and types of
soil.
# Soil composition, grain size and aggregation determine percolation and water holding capacity
of soil.
# Physical and chemical properties determine type of plants and Animals that survive in a habitat.
Response to environmental condition
Regulation
suspention
organism
conformation
Migration
Adaptation
Regulation
# Organisms maintain homeostasis achieved by physiological and behavioral means
# Thermo regulation and osmo regulation.
Conformation
# Cannot maintain constant internal Environment
# Body temperature and osmotic concentration of body changes with ambient temperature and
concentration of medium.
Migration
# Organism moves away temporarily to another habitat in stressful condition.
e.g.- Migratory birds
Suspension
# Organisms suspend their metabolic activities during stressful condition
# Resume their function at the return of favorable conditions.
E.g. Hibernation of Frog, Reptiles, Polar Bear etc
# Aestivation in Snail and Fish.
# Seed dormancy.
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Adaptation
# Morphological, physiological and behavioral changes that enable organisms to adjust to the
ever changing environment .
E.g. Kangaroo rat survives in desert conditions through internal oxidation of fat, removing
concentrated urine of less quantity.
# Allens rule-cold climate mammals have shorter ears and limbs to minimize heat loss.
# Polar mammals like seals have blubber to prevent heat loss.
# Burrowing habit to escape form heat
# Higher count of RBC, Hb at high altitudes.
Population attributes
*Birth Rate Number of individuals born per thousand per year.
*Death Rate Number of individuals die per thousand per year.
*Sex Ratio Ratio of male-female in the population.
*Population density.
Age pyramids
# Three ecological ages:
# Pre-reproductive, Reproductive and Post-Reproductive
# High proportion pre-reproductive individuals occur in expanding population
# Pre-reproductive individuals are uniform in stable population.
# Pre-reproductive individuals are less in Declining population.
Representation of age pyramids for human population

Post-Reproductive
Reproductive
Pre-Reproductive
EXPANDING
STABLE
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DECLINING
Population growth
Factors that affect the size of population

Food availability
Weather
Predation pressure
Competition
Density of population at any time at a given place depends on
Natality, Mortality, Emigration Immigration
Population growth models

Refer to NCRT text book Pg.No.230
Factors that affect population density
IMMIGRATION
[I]
+
NATALITY
[B]
POPULATION DENSITY
[N]
MORTALITY
[D]
EMIGRATION
[E]
Types of population interactions

INTERACTION
Mutualism
Predation
Parasitism
Commensalism
Competition
Ammensalism
SPECIES a
+
+
+
+
-
SPECIEC b
+
0
0
Mutualism
Both the species get benefited.
Lichens Relationship between Non-photosynthetic Fungus and
photosynthetic Algae or Cyanobacteria.
Mycorrhiza Asociation between Fungui and Higher Plants like Pinus.
Plants and insects for pollination
Orchid ophrys and male bee a good example for co-evolution of plants and
Animals.
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PREDATION
One species get benefited and the other harmed.
Tiger and Deer
Snake and Frog
Herbivores and plants
Competition
Both the species are harmed.
Flammingoes and resident fishes compete for the common food zooplankton in
South American lakes.
Abington Tortoise and goats in
Galapagos Islands for food.
Gouses Competitive Exclusion
Principle -Two closely related
species competing for the same
resource
cannot
co-exist
indefinitely
and
the
competitively inferior one will be
eliminated eventually.
Parasitism
One species gets benefit and the other is harmed.
Parasites
Endoparasites
Liver fluke, plasmodium
ectoparasites
lice, ticks
brood par asites

koel
Adaptations of parasites
# Loss of sense organs
# Presence of adhesive organs or suckers
# Loss of digestive system
# High reproductive capacity.
Ammensalism
One species hurts the other but the other is not affected.
Penicillium secretes Penicillin and kill Bacteria but by this Penicillium does not benefit.
Algal bloom leads to death of fishes, but the death of fishes is of no use to the algal bloom.
Commensalism
One species benefits and the other neither harmed nor benefited.
The cattle egret catches the insects disturbed by moving cattle, but the cattle neither harmed nor
benefited.
Another example
The clown fish gets protection from predators by close association with sea anemone, but the sea
anemone is not effected.
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Short answer type questions (3 marks)

1. What is brood parasitism? Give an example. What adaptation has evolved in this phenomenon?
Ans. One species lays eggs in the nest of another bird, lets the host incubate them. e.g. Cuckoo lays eggs
in the nest of a crow.
The Eggs of the parasite resemble the eggs of the host in colour, size. Reduce chances of the host
bird detecting the foreign eggs and ejecting them from nest.
2. Name and explain the kind of interaction in the following.
Ans. 1. Algae and Fungi in Lichens
2. Head Louse Humans
3. Hermit Crab and Sea Anemone
(i) Interaction of mutualism where the two species are equally benefited. Fungus provides protection,
helps in absorption of water and minerals, Algae provide food for the Fungus.
(ii) This is case of Parasitism where the louse is an ectoparasite. Parasite takes shelter on humans and also
derives nutrition.
(iii) It is commensalisms where one species is benefited and the other is neither benefited nor affected. Sea
Anemone is benefited as it does not have to move to places rich in nutrients, while hermit crab is neither
benefited nor harmed.
3. How does Ophrys get pollinated by bees?
Ans.1. Sexual deceit.
2. One petal resembles female.
3. Male pseudocoupulates with the flower.
4. Pollen grain transferred from one flower to another.
4. Biomass is a more meaningful measure of population size. Explain with an example.
Ans. (i) Population large Total number is not an easily adoptable measure. Counting takes long time or
practically impossible
(ii) There is no need to know the absolute population size for some investigations.
(iii) Number may sometimes be misleading e.g. In a given area there are 200 Parthenium plants and a
single banyan tree. Here biomass size of the banyan tree is much more than those of 200 Parthenium
plants.
5. Give example of how plant protects themselves from the predators.
Ans. (i) Thorns. E.g. Rose, babool etc.
(ii) Chemicals that can kill the animals. E.g.- Calotropis etc.
6. What is interference competition? Define competitive exclusion principles.
Ans. (i) Feeding efficiency may be reduced due to interference of another species. E.g. Tiger and deer.
(ii)Two closely related species need same resource can not co-exist indefinitely.
(5 Marks) Questions:
1.What are the different types of population growth pattern? Mention their differences.
Ans: a. Logistic and Exponential growth
b. S Shaped curve, J shaped curve. Limiting Factors, No-limiting Factors
2.With the help of age pyramids explain the nature of a population.
Ans: a. Pre-reproductive/ re-productive/ post-reproductive
b. increasing population/ stable population/ declining population
108
CHAPTER 14.
ECOSYSTEM
QUESTIONS
[2 MARKS QUESTIONS]
Q1.What are decomposers? Write their function.
Ans-a)Saprotrophs feed on dead bodies of organisms, b) Decomposition and mineralization.
Q2.What is the difference between gaseous and sedimentary cycle?
Ans-a) Gaseous-Reservoir in atmosphere ,Nitrogen cycle b) Sedimentary-Soil,e.g-phosphorus.
Q3.Why is the length of a food chain in an ecosystem generally limited to 3-4 trophic levels?
Ans As 90% energy is lost in the form of heat from one trophic level to another, residual energy decreases
drastically within 2-3 trophic levels.
Q4.What are the differences between detritus and grazing food chains?
Ans-a) Begins with Detritus-dead and decaying organic matter. b) Grazing-Begins with Living green plants.
Q5.What are the two basic catagories of ecosystem? Give example.
Ans-a) Terrestrial-Forest, grassland, desert. b) Aquatic-Pond, lake, sea, ocean
Q6.Mention two factors by which productivity is limited in an aquatic ecosystem.
Ans-a) Light-decreases with increasing water depth. b) Nutrient Limiting factor in Deep Ocean
Q7.What is food chain? Give an example.
Ans-a) Food and feeding relation among organisms makes a chain like structure b) GrassDeerLion
Q8.Expand PAR, How much PAR is used in gross primary productivity?
[ 3 MARKS QUESTIONS]
Q1.Briefly describe the process and products of decomposition.
Ans-Breakdown of complex organic matter by decomposers.a)Process-i)fragmentation ii)leaching
iii)catabolism. Humification and mineralization humification leads to accumulation of dark colour substance
called humus. Mineralisation result in release of inorgranic substances.
Q2.Give account of factors affecting the rate of decomposition.
Ans-a) climatic factor i)temp ii) soil b) chemical quality of detritus Higher temp and moist condition high
rate of decomposition Dry soil , High temp Low rate
Q3) What are ecological pyramids ? Mention its limitations .
Ans a) Arrangement of trophic levels from producers to top carnivores forms pyramid like structure 3 types
i) Pyramid of number ii) Biomass iii) Energy
Limitations i) Assumes simple food chain ii) Single species may operate at two or more trophic levels.
Q4 ) Explain carbon cycle with ray diagram .
Ans Given in text.
Q5 .Describe pond as an ecosystem .Ans- Pond has biotic and abiotic components
a) Biotic Phytoplankton ,Zooplankton , small fishes , large fishes , frogs , snake ,etc.
b) Abiotic - water , dissolved organic and inorganic substances ,sunlight , temp .
Phytoplankton (microscopic plants ) producers . Zooplankton (microscopic animals ) primary consumers
Small fishes - secondary consumers Large fishes , frog, snails tertiary consumers.
109
[5 Marks Questions]
Q1. Describe the major components of ecosystems.
Ans- a) Biotic-i) Producer-green plants. ii) Consumers-primary, secondary,tertiary and decomposers. b)
Abiotic-i) Physical and climatic factors-soil, temperature,light, humidity. ii) Chemical factors-inorganic
chemical substances (sodium, potassium, nitrogen etc.) organic substances-(humus, protein, fat etc.)
Q2. Give an account of energy flow in an ecosystem.
Ans- Rate of energy transfer between the organisms of different trophic levels is called energy flow. Energy
flow is unidirectional, 10% loss of energy in each trophic levels. 2-10% PAR captured by green
plants.Energy flow diagram from the text.
Q3.What is xerosere?
Describe the process of succession on a bare rock. Ans-a) Succession on bare rock. b) Steps in Xerosere
i) Lichens-Pioneer Community. ii) Mosses iii) Herbs iv) Shrubs v) Trees-Climax community.
110
Chapter-15: BIODIVERSITY AND CONSERVATION

Biodiversity is defined as the totality of genes, species and ecosystems of a given region, i.e. the combined
diversity at all levels of biological organisation. It is the variety and variability of life form (all animals, plants
and microbes on earth) and the ecological complexes in which they occur. The term was first coined by
Walter G. Rosen(1985),however the term was popularized by the American sociobiologist Edward
Wilson(1988)
Hierarchial levels of Biodiversirty:
1)Species Diversity 2)Genetic Diversity and 3)Ecosystem/Community/Habitat Diversity
Patterns of Biodiversity:1) Lattitudinal gradients Generally, species diversity decreases, as we
move from equator to poles.e.g. However , Tropics(23.50N -23.50S) show richest species diversity.
Speciation is generally a function of time. Temperate region is subjected to glaciation. Tropical regions
remained relatively undisturbed for millions of years and thus had a long evolutionary time for species
diversification. Moreover, tropical environments are less seasonal, relatively more constant and predictable.
Such constant environment facilitates niche specialization and lead to greater species diversity. Tropical
latitudes also get huge solar radiations which promotes higher productivity
SPECIES Area relationships :
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ALEXANDER VON HUMBOLDT observed within a region species richness increased with increasing
explored area but only up to a limit.
The relation between species richness and area for a wide variety of taxa turns out to be a rectangular
hyperbola.
On a logarithmic scale the relationship is a straight line describe by the equation
LogS = logC +Z log A
Where S= species richness, A = Area, Z = slope of the line(regression coefficient),C = Y- intercept.
It has been noted that regardless of the taxonomic group or region the slope of the regression line are
amazingly similar. However, for a very large area like the entire continent the slope of the line is
steeper.
Loss of biodiversity:
Loss of biodiversity in a region may lead to
1)decline in plant production
2)lowered resistance to environmental changes such as drought.
3)increased variability in certain ecosystem processes such as plant productivity, water use, pest &
disease cycles.
Major causes of biodiversity loss:
i)Habitat loss and fragmentation ii)over exploitation iii)Alien species invasions iv)Co-extinctions
and mass extinctions, , v) overexploitation , vi) urbanization, vii)pollution viii) Global climate change
Biodiversity conservation
Reasons for conservation can be grouped into three categories:
a)narrow utilitarian-for deriving direct economic benefit from nature.
b)broad utilitarian-as biodiversity plays a major role in many ecosystem services.
c)ethical-we need to realise that every species has an intrinsic value and we need to pass on our biological
legacy to future generations.
How to conserve biodiversity:
In-situConservation Threatened /endangered plants and animals are provided with urgent
measures to save from extinction within their natural habitat( in wildlife sanctuaries, national parks &
biosphere reserves, sacred groves /lakes-i.e. in protected areas)
Biodiversity hotspots regions with very high levels of species richness and endemism. Norman Myers
developed the concept of hotspots in 1998 to designate priority areas for insitu conservation. They are the
most threatened reservoir of biodiversity on earth. In India 2 hotspots are there,e.g.Western ghats, and the
Eastern Himalayas.
Ex-situ Conservation Threatened animals & plants are taken out from their natural habitat &
placed in a setting where they can be protected and given care as in botanical gardens, zoological
gardens, seed/pollen/gene banks etc.
Efforts to conserve biodiversity:
Convention on Biological Diversity(CBD)
The three main goals of CBD are
1)Conservation of biological diversity
2)Sustainable use of components and
112
3)Fair and equitable sharing of benefits

Indian efforts:
Taking cognizance of the provisions of the CBD,India has enacted an umbrella legislation called the
Biological Diversity Act,2002 and also notified the Biological Diversity Rulkes,2004.Its primary aim is to
endorse the main goals of CBD suiting to Indias national needs and circumstances.
India will host the 11th Conference of Parties(COP) (known as RIO+20)in October2012.
Questions:
Q1. Define Biodiversity.
Q2.What is ecosystem diversity?
Q3.Expand the term IUCN.(International Union for Conservation of Nature and Natural resources)
Q4.Who popularized the term biodiversity?
Q5.Can you mention the estimated number of species so far identified on earth?
Q6. Establish the relationship between species richness and explored area.(comment on the species
area relationship curve).
Q7.Plots with more species showed less year to year variation in total biomass-who showed this? (David
Tilman)
Q8.Who proposed the Rivet popper hypothesis? Comment on the major postulate of this hypothesis. (Paul
Ehrlich)
Q9.Mention the major causes behind biodiversity loss.
Q10. Why should we conserve biodiversity?(comment on the broad/narrow utilitarian and ethical value of
biodiversity)
Q11.What do you mean by the term ecosystem services?
Q12. What is meant by the term endemism?
Q13.What are hot spots? Name two factors for declaring a hot spot. What are the hot spots found in India?
Q14. Distinguish between in-situ and ex-situ conservation measures with examples.
Q15.Can you mention some national and international efforts towards biodiversity conservation?
Q16. Write short notes on i) sacred groves and ii) traditional ecological knowledge.
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Chapter 16: Environmental Issues

Pollution: Any undesirable change in physical, chemical or biological characteristics of air, land, water or
soil which harms the human beings.
POLLUTION
AIR POLLUTION
SOIL POLLUTION
WATER POLLUTION
NOISE POLLUTION
Pollutants: Agents that bring about pollution E.g. smoke, dust, pollen, chemical pollutants, wastes from
hospitals, E-wastes etc.
Biodegradable and non -biodegradable pollutants
Ways of removing particulate matter
1. Electrostatic Precipitator
2. SCRUBBER
3. Proper maintenance of Automobiles
Reference Fig 16.1 NCERT
Advantage of CNG over diesel
CNG burns most efficiently.
Cheaper Cannot be siphoned.
Cannot be adulterated.
Problems in use of CNG
Difficulty in laying down pipelines Non-assurance of uninterrupted supply
Steps taken in Delhi to reduce pollution.
Phasing out old vehicles.
Use of unleaded petrol.
Use of low sulphur Petrol and Diesel.
Use of catalytic converters in vehicles Application of stringent pollution level norms for
vehicles.
Noise pollution
It is undesirable high level of sound.
Harmful effects of noise pollution
Psychological and Physiological disorders
Damage of eardrums and hearing ability
Cause Sleeplessness, increased heartbeat altered breathing pattern, stress etc.
Steps to be taken to control noise pollution
Use of sound absorbent materials or by muffling noise in industries
Demarcation of horn free zones around hospitals and schools.
Permissible sound levels of crackers,
Timings after which Loudspeakers cannot be played
114
Water pollution
Biological Oxygen Demand (BOD) indicates the amount of dissolved oxygen utilised by the
microorganisms for oxidising the oxdisable organic matter present in the water body.
Greater the organics, greater would be the pollution and lesser the dissolved oxygen.
Effects of BOD
Algal bloom
It is free floating (Planktonic) Algae.
Imparts a distinct colour to water bodies
Cause deterioration of water quality and fish mortality.
Some blooms are toxic to humans and Animals.
Water hyacinth (Eichornia crassipes)
Worlds most problematic aquatic weed
Called as Bengal TerrorGrows faster than our ability to remove.
Bio magnification
Increase in concentration of the toxicant at successive tropic levels
Bio magnification of DDTIN Aquatic food chain
Water
Zooplankton
Small Fish
Large Fish
Fish-eating Birds
0.0003 ppm
0.04 ppm
0.5 ppm
2 ppm
5 ppm
Eutrophication
Natural ageing of lake by biological enrichment of its water.
Cultural or accelerated eutrophication
Acceleration of ageing process of a lake by effluents from industries and homes.
Integrated waste water treatment in arcata
It consist of two steps
a) Conventional sedimentation, filtering and chlorine treatment,
b) Passing this water through marshes for neutralization absorption and assimilation of pollutants.
c) Upkeep of this project by FOAM (Friends of Arcata Marsh).
SOLID WASTES
Biodegradable
Recyclable
Non-Biodegradable
Ecological sanitation (Ecosan)

A sustainable system for handling human excreta without using water but with composting method.
Advantages of ecosan
a) Wastage of water is reduced
b) Practical and efficient
c) Hygienic and cheap
d) Excreta can be recycled and used as natural fertilizer.
Hospital wastes
Syringes, discarded medicines, Used gloves, Post-operative materials etc.
Should be treated before disposing off.
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E-wastes
Unused or damaged computers, calculators, mobile phones etc.
Developed countries have plants for recycling e-wastes for recycling of metals.
In developing countries e-wastes are buried in landfills or incinerated.
Agro chemicals
Chemicals used in agricultural fields, Fertilizers, pesticides, weedicides etc.
They are toxic to even non target organisms.
Excess fertilizers cause Eutrophication.
They cause soil pollution
Advantages of organic farming
Economical Wastes do not get accumulated but recycled
Does not cause Eutrophication
Radioactive wastes
Emit radiations and damage biological organisms.
Nuclear wastes are called potent pollutants, as they are lethal even in lower doses.
Disadvantages of nuclear plants
Accidental leakages may happen
Unsafe disposal of radioactive wastes
Radiation emitted cause mutations in organisms
Radiation causes genetic disorders
Greenhouse effect
Earths atmosphere acts as a cover, which allows heat and light to enter in, but heat cannot
escape. Thus warming up the earth.
Green House Gases:-Carbon dioxide, methane etc.
Ozone
Triatomic molecule of oxygen.
Found in stratosphere of atmosphere.
CFCs discharged from lower atmosphere move upward
UV rays act on these CFCs and release chlorine atoms.
Chlorine degrades ozone and release molecular oxygen
This process is irreversible and thus ozone is depleted
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OZONE HOLE
Reference Fig 16.8 NCERT
Soil erosion
The removal of top fertile layer due to human activities
Reasons: Over cultivation
Over grazing
Deforestation
Improper irrigation practices
Waterlogging
The crops may droop
Leads to salinity of the soil.
Slash and burn agriculture/jhum cultivation
Farmers cut down the trees of the forest and burn the plant remains.
Ash is used as fertilizer and land is used for farming or cattle grazing
Later, Land is left uncultivated for several years for replenishment of minerals
Effects of deforestation
Leads to global warming due to excess carbon-dioxide
Loss of biodiversity
Damage to hydrological cycle
Leads to soil erosion
Desertification of land
Reforestation
Restoring forest that was existing earlier
E.g. Observing Van-Mahotsavas
It also occurs naturally
Afforestation
Developing a forest in a new area where no such forest existed in that area.
A case study of peoples participation in forest conservation
A king of Jodhpur wanted to arrange wood for his new palace in 1731.
Few Bishnois hugged the trees and asked to cut them first rather than cutting trees.
365 persons lost their lives in this act
A small temple is now present there in remembrance of this act
Amrita Devi Bishnoi Wild Life Protection Award is instituted for individuals of rural areas who take keen
interest in protecting wild life.
Chipko movement
It was started by local women of Garhwal, They hugged the trees to protect them from the axes of
contractors.
Joint forest management (jfm)
Startedy Government of India in 1980
Local communities worked with the government to save the forest.
Communities get forest products for encouragement.
117
Environmental issues
Very short answer type questions (1mark)
1. What is meant by algal blooms? What is its significance?
Ans. Excess growth of certain phytoplankton due to excess nutrients in water causes Deteriorates water
quality, leads to fish mortality.
2. Define eutrophication.
Ans. Nutrient enrichment in water bodies leading to depletion of oxygen and loss of life supporting
Environment.
3. What is bio magnification?
Ans. Increase in the concentration of certain toxic chemicals at successive trophic levels.
4. What is BOD?
Ans. Biological Oxygen Demand is the measure of organic matter in any water sample.
5. What is the effect of DDT in birds?
Ans. DDT disturbs calcium metabolism in birds, thinning of egg shell and premature breaking of
Eggs lead to decline in bird population.
6. What do you understand by Ecosan?
Ans. Ecosanare the toilets which use compositing method for ecological sanitation.
7. Why are nuclear wastes called potent pollutants?
Ans. Because they are lethal even at lower doses and cause damaging disorders.
8. What is Jhum cultivation?
Ans Farmers cut down the tress, burn, use cattle for grazing and then allow the land to recover.
9. Mention two problems that have arisen due to green revolution.
Ans. Water logging and soil salinity.
10. What is snow blindness?
Ans. Inflammation of cornea caused by a high dose of UV-B radiation.
11. Which is the worlds most problematic weed, also known as terror of Bengal?
Ans. Eichorniacrassipes(Water hyacinth).
12.. What is the effect of DDT in birds?
Ans. Disturbs Calcium metabolism Thinning of egg shells and premature breakage of eggs, Decline
of bird population.
1. Mention the harm caused by fine particulate matter to human beings?
Ans. (i) Cause respiratory problems
(ii) Irritation of eyes
(iii) Inflammation of lungs
(iv) Premature death.
2. Differentiate between biodegradable and non-biodegradable wastes.
Biodegradable wastes
Non-Biodegradable wastes
*Can be broken down into harmless simple
*Cannot be broken down by microbes and get
Compounds by the action of decomposers.
accumulated in the biosphere
*Can be used as manure
*Enter the food chain
*Cause little pollution
*Cause bio magnifications
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3. Describe Chipko Movement.

Ans. It was launched in Garwhal, Himalayas by Sh Sunder LalBahuguna in 1974.
Local women showed enormous bravery in protecting the trees from the axes of the contractors
by hugging them.
4. What are the advantages of Organic farming?
Ans. Economical procedure as recycling takes place.
Waste not accumulated but recycled
Efficiency and utilization of resources increased
Does not lead to eutrophication.
5. Write an account on Ecological sanitation (Ecosan).
Ans.A sustainable system for handling human excreta, using dry composting toilets. Practical,
Hygienic, efficient and cost-effective solution to human waste disposal Human excreta can be
Recycled into manure Used in Kerala and Sri Lanka.
6. How do radioactive wastes cause damage to living organism?
Ans. Cause mutations in living organisms at a very high rate. Lethal in high doses Causes cancer
And other disorders.Reduces the vegetation cover.
7. What is ecological sanitation? What are its advantages?
Ans. It is sustainable system for handling human excreta without using water but by composting
Method.
Advantages
Hygienic, practical and efficient, Conserves water can be recycled and, Acts as a natural fertilizer.
1. Mention harmful effects of noise pollution on human health.
Ans. Stress Altered breathing pattern
Increased heart beating and blood pressure
Sleeplessness and headache
Hearing impairment.
2. What measures should be taken to reduce global warming?
Ans. Reduce use of fossil fuel
Efficient use of energy.
Avoid deforestation
Reduce human population Control greenhouse gases.
3. How can we reduce automobile pollution?
Ans. Un-Leaded Petrol- Reduces lead pollution in air.
Low Sulphur Diesel- Reduces sulphur pollution in air
Four stroke engines to reduce emission of unburnt hydrocarbons.
Tube-Ups to increase air-fuel ratio and help in better combustion.
Catalytic Converters to reduce pollution.
CNG to reduce pollution and conserve fossil fuels.
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4. Mention the adverse effects agrochemicals.

Ans. They are toxic to non-target organisms. They cause soil pollution Excess fertilizers cause
eutrophication.
5. Write a short note on ozone depletion.
Ans. Ozone found in stratosphere. CFCs discharged from lower atmosphere move upward. In
stratosphere UV rays act on these CFCs release chlorine atoms. Chlorine degrades ozone and
release molecular oxygen (O3O2). In this reaction chlorine acts, as catalyst and loss ozone is
irreversible.
6. Mention the Supreme Court directions to the Government to reduce pollution.
Ans. Switch over to CNG in public transport system
Enforcement of Euro II norms for vehicles.
Compulsory periodic check-up of pollution.
Use of unleaded petrol Low sulphur petrol and diesel
Catalytic converters in vehicles
Phasing out of old vehicles.
Long answer type questions (5marks)
1. a) Explain the functioning of electrostatic precipitator with the help of a diagram.
b) Mention the consequence if the electrostatic precipitator does not work in a power plant.
Ans. Used for removing particulate air pollutants.
Removes about 99 of the particulate pollutants from the exhaust of thermal power plants.
Electrode wires that are maintained at several thousand volts, which release electrons.
Electrons become attached to dust particles giving a net negative charge.
Collecting plates are grounded and attract the charged dust particles.
Velocity of air between the plates must be low enough to allow the dust particles to fall.
If electrostatic precipitator of a thermal plant stops working, all the particulate pollutants get released
and pollute the air.
Discharge corona
Negatively charged wire
Dirty Air
Clean air
Dust particles
Collection plate grounded
ELECTROSTATIC PRECIPITATOR
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WORD LIST BINGO

Chapter-1:
REPRODUCTION IN ANIMAL
Life span, reproduction, asexual reproduction, clone, binary fission, buds, zoospores, conidia, gemmules,
vegetative propagation, runner, rhizome, sucker, tuber, offset, bulb,vegetative propagules, nodes,sexual
reproduction, seasonal flowering, oestrous cycle, fertilization, gametogenesis, isogametes, heterogametes,
antherozoid, homothallic, monoecious, hetrothallic, dioecious, staminate,pistillate, unisexual, bisexual,
hermaphrodites, meiocytes, pollination
Syngamy, zygote, parthenogenesis, external fertilization, internal fertilization, embryogenesis, cell
differentiation, oviparous,viviparous,ovary, pericarp.
Chapter-2:SEXUAL REPRODUCTION IN FLOWERING PLANTS

Androecium, Gynoecium, stamen, filament, dithecous, microsporangia, pollen sacs,tapetum, sporogenous
tissue, microsporogenesis, microspore tetrad, pollen grains, exine, intine, germ pores, vegetative cell ,
generative
cell,monocarpellary
multicarpellary,syncarpous,
stigma
,
style,
ovary,placenta,megasporangia(ovule) funicle, hilum, integuments, micropyle, chalaza, nucellus, embryo sac
(female gametophyte), megasporogenesis, megaspore mother cell, megaspore, monosporic, egg apparatus,
synergids, antipodals, egg cells, filiform apparatus, polar nucleus, secondary nucleus, pollination,
autogamy, chasmogamous and cleistogamous flower, geitenogamy, xenogamy, outbreeding devices,
pollen- pistil interaction, artificial hybridization, emasculation, bagging, primary endosperm nucleus, triple
fusion, endosperm , embryo, post fertilization events, scutellum, heart shaped embryo, plumule, hypocotyle,
epicotyle, plumule, radical, coleorrhiza, coleoptiles, albuminous/nonalbuminous seed, perisperm, seed
dormancy, pericarp,false/true /parthenocarpic fruit,apomixes, polyembryony
Chapter-3:HUMAN REPRODUCTION
Insemination, implantation, testes, scrotum, seminiferous tubules, sertoli cells, interstitial cells of Leydig,
rete testis, vasa efferentia, epididymis, vas deferens, urethral meatus, accessory reproductive glandsseminal vesicles, prostate, bulbourethral glands, ovaries, oviduct, uterus, cervix, vagina, mammary glands,
infundibulum, fimbriae, ampulla, isthmus, perimetrium, myometrium, endometrium,fallopian
tube,clitoris,lactiferous duct,spermatogenesis, spermatogonia, primary spermatocytes,secondary
spermatocytes, spermatids, spermatozoa, spermiogenesis/spermateleosis, spermiation ,acrosome, semen,
oogenesis, oogonia, primary oocyte, primary/secondary follicle, antrum, graffian follicle, zona pellucid,
ovulation,
polar
bodies,menstrual
cyclemenarche,corpus
luteum,
progesterone,
menopause,cleavage,morula, blastomeres,trophoblast cells, inner cellmass, implantation, chorionioc villi, h
CG, h PL, estrogen, relaxin, umbilical cord, germ layers-ectoderm, mesoderm, endoderm, stem cells,
parturition, foetal ejection reflex, lactation, colostrums.
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Chapter-4:REPRODUCTIVE HEALTH
Health,STDs,AIDS,Reproductive & child health care, amniocentesis, maternal mortality rate(MMR), infant
mortality rate(IMR), contraceptive,natural methods, periodic abstinence, withdrawal method/coitus
interruptus ,barrier methods, condoms, diaphragms, cervical caps, vaults, intra uterine devices(IUDs), pills,
sterilization, vasectomy,tubectomy,Medical Termination of Pregnancy(MTP), Infertility, Assisted
Reproductive technologies(ART),in-vitro fertilization(IFV), embryo transfer, test tube baby, zygote intra
fallopian transfer(ZIFT),intra uterine transfer(IUT), in-vivo fertilization, gamete intra fallopian transfer(GIFT),
Intra
cytoplasmic
sperm
injection(ICSI),
artificial
insemination(AI),
intra
uterine
insemination(IUI),Adolescence Reproductive and Sexual Health(ARSH), Reproductive Health
Chapter-5:PRINCIPLES OF INHERITANCE AND VARIATION

Inheritance, Fillial progeny, factors,traits, homologous chromosome,
gene, loci/locus, allele
homozygous,heterozygous ,phenotype, genotype, monohybrid cross, dominant , recessive, punnett
square,segregation, dihybrid cross, independent assortment,test cross,back cross, incomplete dominance,
co-dominance, chromosomal theory of inheritance,linkage, recombination,sex determination, autosomes,
sex chromosomes/allosomes,homogametic, heterogametic, mutation, chromosomal aberrations, frame-shift
mutation, pedigree analysis, consanguineous mating, Mendelian disorders, nondisjunction, haemophilia,
sickle cell anaemia, phenylketonurea, in born error of metabolism/metabolic disorder,aneuploidy,polyploidy,
syndrome, Downs syndrome, trisomy, Klinefeltors syndrome, gynaecomastia, Turners syndrome,
rudimentary/streak gonad.
Chapter-6:MOLECULAR BASIS OF INHERITANCE

Deoxyribonucleic Acid(DNA), Ribonucleic Acid(RNA),nucleotide, nucleoside, nitrogenous bases(purine,
pyrimidine), sugar(pentose-deoxyribose/ribose),Adenine,Guanine, Thymine, Cytosin,Uracil, N-glycosidic
linkage, Phosphodiester linkage, double helix, Chargaffs rule, anti parallel polarity, central dogma, histones,
octamer, nucleosome, chromatin, euchromatin, heterochromatin, nonhistone chromosomal
protein(NHC),Transformation(Griffiths experiment),
Transduction
(Hershey-Chase
experiment),
replication,semiconservative replication,template DNA, Meselson-Stahls experiment,replication fork,
helicases, topoisomerases, single stranded binding protein, primase,DNA polymerase, DNA ligase,Okazaki
fragments, continuous (leading)strand, discontinuous (lagging)strand, Transcription, promoter,structural
genes/cistrons,terminator,DNA dependent RNApolymerase,coding strand, polycistronic, monocistronic,
coding sequence(exon),non-coding sequence(intron), messengerRNA(m RNA), transferRNA(t
RNA),ribosomal RNA(r RNA), Initiation factor, termination factor, Eucaryotic transcription, heterogenous
nuclear RNA(hn RNA),capping,tailing, splicing,Translation, genetic code,codon, unambiguous, degenerate,
universal, methionine/fMet, START/initiation codon(AUG), STOP/NONSENSE CODON(UAA,UAG,UGA),frame
shift (insertion/deletion) mutation, adapter molecule, untranslated region,anti codon, aminoacylation of t
RNA, release factor,regulation, gene expression,operon, regulator, promoter,operator,lac-Operon, Bgalactosidase,permease,trans-acetylase,lactose,inducer,switch on/off, inducible system,negative regulation,
Human Genome Project (HGP),GENOME, bioinformatics, DNA sequence, Expressed Sequence Tags(ESTs),
Sequence Annotation
122
Bacterial
artificial
chromosome(BAC),Yeast
artificial
chromosome(YAC),Single
nucleotide
polymorphism(SNPs), DNA fingerprinting, DNA polymorphism, repetitive DNA, satellite DNA, Variable
Number Tandem Repeats(VNTRs),isolation of DNA, Electrophoresis, blotting, hybridization, probe,
autoradiography.
Chapter-7:EVOLUTION
Evolution, Big bang, spontaneous generation, panspermia, Millers experiment, HMS Beagle,
CharlesDarwin,fitness,AlfredWallace,fitness,naturalselection,evidences,morphology,anatomy,homologous
organ, divergent evolution, analogous organ, convergent evolution, industrial melanism, adaptive radiation,
branching descent, saltation, stabilising selection, directional selection, disruptive selection, gene migration,
gene flow, genetic drift, mutation, genetic recombination, founder effect, geological periods, Dryopithecus,
Ramapithecus, Homo habilis, Homo erectus, Homo sapiens, Neanderthal man, brain capacity.
Chapter-8:HUMAN HEALTH AND DISEASES

Health, disease ,infection, genetic disorders, life style disorders, infectious/ non-infectious, pathogen,
typhoid, widal test, pneumonia, common cold, malaria, Plasmodium sp. Anopheles sp. ,Haemozoin,
sporozoites, gametocytes,
amoebiasis,
ascariasis, elephantiasis/filariasis, Wuchereria bancrofti,
ringworms, personal hygiene, public hygiene, air borne disease, water borne disease, vector borne diseases,
biological control(Gambusia), Aedes, immunity, innate immunity, physical barrier, physiological barrier,
cellular barrier, cytokine barrier, acquired immunity, B lymphocytes,T ,lymphocytes,
antibody(Immunoglobulin), light chain, heavy chain, Humoral immune response, cell mediated immunity,
active immunity, passive immunity, colostrums, IgA, vaccination, immunization, allergies, IgE, histamine,
serotonin, auto immunity, lymphoid organs, bone marrow, thymus, mucosal associated lymphoid
tissue(MALT),Acquired Immuno Deficiency Syndrome (AIDS),retro virus, HIV, Enzyme Linked Immuno
Sorbent Assay(ELISA),Cancer, contact inhibition, benign tumor, malignant tumor, neoplastic cells,
metastatis, carcinogens, viral oncogenes ,proto oncogenes, radiotherapy, chemotherapy, immunotherapy,
a-interferon ,drugs, opioids, cannabinoids, cocaine, barbiturates, amphetamines, LSD, hallucinogens, drug
abuse, addiction, dependence, withdrawal syndrome, alcohol abuse , liver cirrhosis, danger signs, peer
pressure.
Chapter-9:STRATEGIES FOR ENHANCEMENT IN FOOD PRODUCTION

Animal husbandry, dairy, poultry, animal breeding, inbreeding, out breeding, homozygosity, inbreeding
depression, out breeding, out crossing , cross breeding, interspecific hybridization, artificial insemination,
Multiple Embryo Transfer Technology(MOET), apiculture, fisheries, plant breeding, green revolution,
germplasm collection, cultivars, disease resistance, mutation breeding, insect pest
resistance,
biofortification, Single Cell Protein(SCP), tissue culture, explants, totipotency, micropropagation,
somaclones,meristem, somatic hybrids.
123
Chapter-10:MICROBES IN HUMAN WELFARE

Microbes, Lactic acid bacteria(LAB), Saccharomyces cerevisiae, fermentors, distillation, antibiotics,
bioactive molecules, streptokinase, clot buster, cyclosporine A, immunosuppressive agents, statins,
cholesterol lowering agents, sewage, primary treatment, primary sludge, flocs, Biochemical Oxygen
Demand(BOD), Activated sludge, anaerobic sludge digesters, biogas, Ganga action plan, Yamuna action
plan, methanogens, Bt cotton, Bacillus thuringiensis, baculoviruses, Trichoderma spp. Integrated Pest
Management(IPM), Biofertilisers, organic farming, mycorrhiza, cyanobacteria.
Chapter-11:BIOTECHNOLOGY:PRINCIPLES AND PROCESSES

Biotechnology, genetic engineering, recombinant DNA, gene cloning, gene transfer, origin of replication,
plasmid, restriction enzymes, cloning, restriction endonuclease, recognition sequence, nucleases,
exonucleases, endonucleases, palindrome, sticky end ,blunt end, gel electrophoresis, elution, cloning
vectors, selectable markers, transformation, antibiotic resistance, insertional inactivation, tumor, Ti plasmid,
Agrobacterium tumifaciens, micro injection, biolistic/gene gun, lysozyme, cellulose, chitinase, Polymerase
Chain Reaction(PCR),denaturation, annealing, extension, thermostable DNApolymerase, bioreactors,
downsteam processing.
Chapter-12:BIOTECHNOLOGY AND ITS APPLICATION

Genetically Modified Organism(GMO), Bt cotton, insecticidal proteins, cry genes, pest resistant plants, RNA
interference(RNAi)/RNA silencing, dsRNA, Genetically engineered insulin, gene therapy, ADA deficiency , c
DNA, Molecular diagnosis, transgenic animals, Bioethics, Genetic Engineering Approval Committee(GEAC),
Bio piracy, Indian patent bill.
Chapter-13:ORGANISMS AND POPULATIONS

Organisms, population, communities, ecosystems, biomes, ecology, grassland,tundra, desert, coniferous
forest, temperate forest, tropical forest, abiotic features, temperature,stenothermal, eurithermal, water,
salinity, pH, light, soil, moisture,conformers, regulators, partial regulators,migration, suspension,
hibernation, aestivation,adaptation,altitude sickness, Allen,s rule
population attributes,age
pyramid(expanding, stable, declining),population density, natality, mortality, immigration, emigration,
exponential growth, logistic growth,mutualism, competition, predation, parasitism, commensalism,
amensalism.
Chapter-14:ECOSYSTEM
Terrestrial ecosystyem, aquatic ecosystem, stratification, productivity, Gross primary productivity (GPP),Net
primary productivity(NPP),Secondary, productivity,decomposition, detritus, detritivores, fragmentation,
leaching, catabolism, humification, humus, mineralization, Photosynthetically active radiaton(PAR),energy
flow, producers,consumers,herbivores,carnivores,Grazing food chain(GFC), Detritus Food Chain(DFC), food
web, trophic level, standing crop, biomass, Ecological pyramids, upright pyramid, inverted pyramid,
succession, pioneer, sere/seral stage, climax community, Hydrarch succession, Xerarch succession,
nutrient cycling , biogeochemical cycles, ecosystem services.
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Chapter-15:BIODIVERSITY AND CONSERVATION

Biodiversity, genetic diversity, species diversity, ecological/habitat diversity,mega diversity country, loss of
biodiversity,habitat
loss, habitat fragmentation, over exploitation, alien/non native/invasive species,
coextinctions, narrowly utilitarian, broadly utilitarian,in situ conservation, endemism, hotspots, sacred
groves, Ex situ conservation,Earth summit, sustainable development.
Chapter-16:ENVIRONMENTAL ISSUES
Pollution, pollutants, Environmental Protection Act(EPA), Electrostatic Precipitator(ESP), Vehicular pollution,
Compressed Natural Gas(CNG), Euro ii, Bharatii, Air prevention and pollution control act, noise pollution,
decibel, water pollution, domestic sewage, dissolved oxygen(DO), Oxygen sag curve, biochemical/biological
oxygen demand(bod), algal bloom, planktonic, Bioaccumulation, Biomagnificationj,solid wastes, municipal
solid waste, sanitary landfills, plastic waste, e-wastes, agro-chemicals, radioactive wastes, enhanced
Greenhouse effect , Global warming, CFCs, stratospheric Ozone depletion, deforestation,slash & burn
agriculture, Jhum cultivation, deforestation, reforestation, chipco movement, Joint Forest Management(JFM)
125
QUESTION
CLASS : XII (Sc.);
M.M.:70 ;
SUBJECT: (BIOLOGY)
TIME: 3 HRS.
General instructions:
1. All question are compulsory.
2. The question paper consists of four sections A,B,C&D.Section A contains 8 questions of one mark
each, Section B contains 10 questions of two marks each, Section C contains 9 questions of three
marks each & Section D contains 3 questions of five marks each.
3. There is no overall choice.However, internal choice is provided in few question where student
should attempt only one of the alternative given.
4. Wherever necessary, neat labeled diagram should be drawn.
SECTION : A
Q1.Chromosome number in meiocytes of Apple is 34. Write the chromosome number in gamete ? (1)
Q2. Which kind of evolution is shown by the thorn & tendril of bougainvillea & Cucurbita?
(1)
Q3How water pollinated flowers protect the pollen grains from water ?
(1)
Q4.What kind of chromosomal disorder results in the genetic disorder of Klinefelters syndrome?
(1)
Q5.Some cells release interferons. Why?
(1)
Q6.Name the bio-reactive molecule used as immunosuppressive agent & mention the organism from
which it is produced ?
(1)
Q7.Identify the kind of population interaction in an Orchid growing as an epiphyte on a mango branch? (1)
Q8. What type of growth status the following pyramid represents:
(1)
PC 21
PP
SECTION : B
Q9.Differentiate between Chasmogamous & Cleistogamous flower.
(2)
Q10 Explain the following: i) I V F
(2)
ii) I C S I
Q11.i)What kind of disorder leads to the disease Phenylketonuria?
126
ii) What is the resultant effect of the disorder in man?
(1+1=2)
Q12. Name the missing organisms/ diseases in the table given below.
Organism
Microsporum
B
C
Plasmodium falciporam
(1/2X4=2)
Disease
A
Elephantiasis
Amoebiasis
D
Q13.i) Write the use of Cyclosporin A & write the name of the organism from which it is
produced?
ii)Give one example each of fermented beverages with &without distillation.
Q14. Complete the following gaps in A, B, C & D in the following table :
( 1+1=2)
(2)
Crops
Variety
Resistance to disease
Wheat
Resistant to leaf and stipe rust
Pusa swarnim
Resistant to white rust
Cauliflower
Resistant to black rot
Pusa kamal
Bacterial blight
Q15.i) Expand GEAC.

ii)Mention two modern biotechnological techniques for early molecular diagnosis of diseases.
(1+1=2)
Q16.Differentiate between Exponential &Logistic growth with the help of population growth curve.
(2)
Q17 .Differentiate between in-situ & ex-situ conservation of biodiversity with suitable example
Q18.i) What is e-waste?
(2)
ii)Mention different kinds of impurities present in waste water.
(1+1=2)
SECTION : C
Q19.i) Explain Hardy-Weinberg equilibrium with suitable example.
ii) Mention five factors affecting Hardy-Weinberg equilibrium.
(2+1=3)
Q20. Draw a neat diagram of structure of a Nucleosome & label four parts of it.
OR
Draw a neat diagram of structure of Human sperm & label four of its parts.
127
(3)
Q21. Answer the following about Opioid drugs: i)Name opioid receptors in human body
ii) Give one example of opioid drug iii)How it is extracted iv) How it is taken by man
v)What is its effect on human body? vi) Chemical name of the drug.
Q22. Explain the following in relation to sewage treatment: i) Floc
iii) Anaerobic sludge digesters.
(3)
ii) BOD
(3)
Q23. i)What is Biomagnification?

ii)What is meant by Green house effect?
(1+2=3)
Q24. Explain the steps involved in the process of Decomposition in the ecosystem.
(3)
Q25.How biotechnological application for production of Pest resistant could be developed in Bt cotton
plant.
(3)
Q26. i)Explain the oral administrative contraceptive device & time period of its effectiveness.
ii) Give example of one copper releasing & one hormone releasing IUDs.
(2+1=3)
Q27. Mention the six steps followed in the technique of DNA fingerprinting.
(3)
SECTION : D
Q28.i)Name the male accessory glands & its function in man.
ii)Explain the hormonal changes that occur during Menstrual cycle in human with the help
graphical diagram.
iii)What is Placenta?
of
(1+3+1=5)
OR
i)Explain the stages of Megasporogenesis in flowering plants with the help of suitable diagram.
ii)Draw a neat labeled diagram of mature pollen grain.
iii)Differentiate between Perisperm & Pericarp.
(3+1+1=5)
Q29.i) Draw a neat labeled diagram of a Transcription unit & define the following:
a) Function of RNA polymerase III b) Splicing c) Tailing.
ii)What is Frame-shift insertion?
(3+2=5)
OR
i)
Differentiate between Benign tumour & Malignant tumour.
ii) Mention two methods of detection & diagnosis of cancer.

iii) Write any two methods for prevention of drug abuse in adolescence.
128
(1+2+2=5)
Q30. i) Complete the following palindrome sequence and name the restriction endonuclese that recognizes
this.
5
3
G
ii) Explain three methods for transfer of recombinant DNA in competent host in rDNA technology.
iii) What is meant by Insertional inactivation ?
(1+3+1=5)
QUESTION
CLASS : XI (Sc.);
SUBJECT: (BIOLOGY)
M.M.:70 ; TIME: 3 HRS.

General instructions:
All question are compulsory.
The question paper consists of four sections A, B, C & D.Section A contains 8 questions of one
mark each, Section B contains 10 questions of two marks each, Section C contains 9 questions of
three marks each & Section D contains 3 questions of five marks each.
There is no overall choice.However, internal choice is provided in few question where student
should attempt only one of the alternative given.
Wherever necessary, neat labeled diagram should be drawn.
SECTION : A
Q1.Name the kind of Leucoplast that stores oil & protein?
(1)
Q2. Where do you find Porins?Mention its function.
(1)
Q3.In which stage of cell division chromosome number could be counted easily?
(1)
Q4.Name the following organisms:i) A cyanobacterium used as Protein rich food?

ii) A Dianoflagellate causing Red tide?
(1)
129
Q5.Locate Companion cells in plant tissues & write its function.
(1)
Q6.Name the component parts in mouth of Cockroach.
(1)
Q7.Certain marine brown & red algae produce large amount of hydrocolloids that are commercially used.
Name those two substances.
(1)
Q8.Identify the symbols used in writing the floral formula of a flower :- i) % ii) G
(1)
SECTION : B
Q9.Differentiate between Symplastic & Apoplastic pathway of movement of water in plants.
(2)
Q10. Write one point difference between:

i) Peptide bond & Glycosidic bond
ii) Nucleoside & Nucleotide
(2)
Q11.Fill in the blanks with suitable secondary metabolites/Examples :-
Q12.
i) Pigment
: _____________
ii) ______
: Lemon grass oil
iii)Toxins
: ____________
iv)______
: Rubber
(2)
Write the composition of Fluid Mosaic model proposed by Singer & Nickolson.
(2)
Q13. What is Heterocysts ? Give two examples where heterocyst is found.
(1+1=2)
Q14. Complete the following gaps in A, B, C & D in the following table using suitable words :
(2)
Roots of Rhizophora
Leaves of Calotropis
Fruit of Mango
A scar on seed coat
Q15.i) What is Mesosome? Write its function.

ii) Omnis cellula-e cellula. Illustrate.
(1+1=2)
130
Q16.Differentiate between Areolar tissue & Adipose tissue with suitable example.
(2)
Q17 .Identify the following : i) Periderm ii) Conjoint vascular bundle.
(2)
Q18.Draw a neat labelled diagram of different kinds of chromosomes based on the position of centromere.
OR
Draw a neat labelled diagram of a Bacteriophage.
(2)
SECTION : C
Q19.Mention the events that happen during the following phases of cell cycle:
i) G1 phase ii) S phase iii) Quiescent phase
(3)
Q20. Draw a neat diagram of a monocotyledonous seed & label different parts of it.
OR
Draw a neat diagram of Digestive system of frog & label different parts of it.
(3)
Q21. Answer the following :

i)Name the central cavity in sponges through which water passes.
ii) Name the part in Ctenophores having external ciliated rows meant for locomotion.
iii)Name the specialised cells in flat worms that help in osmoregulation.
iv)Name the kind of scales that cover the skin of bony fishes.
v)Name the part that represent ear in Amphibians.
vi) Name the lateral appendages that help in swimming in Nereis.
Q22. Classify different types of Nephridia in Earthworm & locate each type in it.
(3)
(3)
Q23. The transpiration driven ascent of xylem sap depends on some physical properties of water.
Explain three such properties of water.
(3)
131
Q24. Explain the activity of following enzymes: i) Hydrolase ii) Lyase iii) Transferases.
(3)
Q25.Mention the universal rules to be followed in Binomial nomencleture.
(3)
Q26. Which group of fungi are called as imperfect fungi? State reasons.
(3)
Q27. Mention two ways that Ammonia can be utilized by plants through Nitrogen cycle.
(3)
SECTION : D
Q28.i)Differentiate between Chordates & Non- chordates with suitable examples.
ii)Write the name of the group in which following structures are found & mention function of each:a) Choanocytes b) Malpighian tubules c) Water vascular system
(2+3=5)
OR
i)Explain the steps involved in the process of Double fertilization in flowering plants with the help
of suitable diagram.
ii)What does Haplo-diplontic phases of life cycle signify? Give suitable example.
(3+2=5)
Q29. Describe the floral characters of the family Fabaceae.Write the floral formula & floral diagram of it.
OR
i) Categorise various kinds of Aestivation & explain each with suitable diagrammatic representation.
ii)What is Pericarp?
(3+2=5)
Q30. i)Explain the steps involved in Prophase I of Meiosis I with suitable diagram.
ii) Write the significance of Meiosis.
(3+2=5)
__________________
132
LIST OF IMPORTANT DIAGRAM

Chapter1:
Fig1.2(a) and (b),NCERT,page5
Fig18(a) and (b),NCERT,page16
Chapter2:
Fig2.1,NCERT,page20
Fig2.3(a),(b) and (c),NCERT,page22
Fig2.7(d),NCERT,page25,(anatropous ovule the fig should be turned upside down)
Fig2.8,NCERT,page26
Fig2.12c and d,NCERT,page32
Fig2.13,NCERT,page34
Fig2.15a(maize seed),NCERT,page37
Chapter3:
Fig3.1,NCERT,page43
Fig3.4,NCERT,page46
Fig3.5,NCERT,page47
Fig3.6,NCERT,page48
Fig3.7,NCERT,page49
Fig3.8,NCERT,page49
Fig3.9,NCERT,page50
Chapter6:
Fig6.1,NCERT,page96(for HOTS)
Fig6.12,NCERT,page114(Consult any reference book for correct diagram)
Chapter7:
Chapter8:
133
Chapter10:
Fig10.8,NCERT,page186(HOTS)
Chapter11:
Chapter12:
Chapter13:
Chapter14:
Chapter15:
Chapter16:
Fig16.6 16.7,NCERT,page281(HOTS)
Notes: Diagram should be drawn in pencil, labeled with pencil, a caption depicting the Fig must be
given.
Diagram should be drawn scientifically without stressing on unnecessary shades/colour.
134
HIGH ORDER THINKING (HOTS) QUESTIONS

CHAPTER-2
What is Pollen Viability?How the pollen grains of different species are kept stored?
What is mea
nt by free nuclear endosperm. Give one example.
Name the single cotyledon found in
grass family. Differentiate between coleoptile and coleorhiza?
Production of Hybrid seed is a costly affair then why do farmers produce such seeds every year? How can
this problem be solved.
CHAPTER- 3
Q. Why cleavage is called as fractionating process?
A-Cleavage results in increase in number of blastomeres but decrease in size of blastomeres.
Q. Which factor determines the pattern and speed of cleavage?
A- Amount and distribution of yolk.
Q. Name the extra-embryonic membranes in human embryo and mention its function.
A- Yolk Sac Vestigial, act as extra embryonic gut.
Amnion-Protect embryo, acts as shock absorber, prevents desiccation of embryo.
Allantois- Stores nitrogenous wastes, acts as extra embryonic kidney.
Chorion Helps exchange of gases, acts as extra embryonic lung.
Q. Which type of Placenta is found in man?
A- Chorionic ( finger-like out growth ) , haemochlorial , metadiscoidal(Chorionic villi exposed like disc) and
deciduate (part of uterine wall expelled during parturition ).
Q. Why testis lies outside the body cavity in scrotal sac ?
A- Scrotal sacs act as thermo regulators, keep testicular temperature 2 degrees lower than the normal body
temperature for normal spermatogenesis.
Q. What happens if testes fail to descend into scrotal sac?
A-High temperature of abdomen will kill the spermatogenetic tissues of the testis and no sperm will be
formed (azoospermia) causing sterility , the phenomenon called Crytorchidism.
Q Vitellogenesis is an important phenomenon after fertilization . Give reasons .
A-After fertilization Vitelline membrane is transferred into fertilization membrane which checks polyspermy
Q Penetration of sperm is a chemical process. Illustrate.
A Sperm head i.e. acrosome contains sperm lysin / hyaluronidase enzyme which help dissolving hyaluronic
acid binding the follicular cells of corona radiata from penetration of sperm nuculeus to egg nucleus
Q In morula stage the cells divide without any increase in size why ?
A Since, zona pellucida of egg cell remain intact till completion of cleavage.
Q What is the importance of fertilizinAntifertilizin reaction?
A Ovum secretes fertilizin( glyprotein or mucopolysaccharide ) which has a number of spermophiliic sites
on its surface where sperm can be bound by their antifertilizin site (on sperm head containing acidic amino
acid) In this process thinning out of number of sperms take place to avoid polyspermy.
135
CHAPTER-4
(REPRODUCTIVE HEALTH)
Q.Why oral contraceptive pills are called as combined pills?

Ans.Since the same medicine contains mainly Progestogen& Estrogen that shows combined action
inhibiting ovulation as well as contraception.
Q.Amniocentesis is illegal for detection of sex of foetus.Why?
Ans. The method is misused for identification of female child during foetal development & aborted due to a
misconception of rejection of female child by the society.
Q.Removal of gonads can not be considered as contraceptive options. Why?
Ans. Contraception basically includes preventing unwilling conception without affecting normal body
function that can be disrupted by removal of gonads.
Q. What are the essential features for an ideal contraceptive?
Ans. Ideal contraceptive should be user friendly, comfortable & easy to use, without any side effect &
completely effective against pregnancy.
Q. All RTIs are STDs but all STDs are not RTIs. Justify.
Ans.Reproductive tract infections are basically transferred through sexual contact & hence may be termed
as sexually transmitted diseases whereas some STDs like Hepatitis-B, AIDS are not caused due to
infection of reproductive tract although transmitted through sexual contact.
Q. Now a days number of childless couples is decreasing. Why?
Ans.Various improvised scientific methods are available for infertile couples to have a child through
assisted reproductive technology.
Q.How CVS technique is more advanced than Amniocentesis?
Ans. Chorionic Villi Sampling technique may be applied by 8 th to 10th week of pregnancy whereas
Amniocentesis is done in about 14th to 15th week. Hence, abortion is easier & less risky in CVS techniques.
Q. Test tube baby has raised several legal problems. Explain.
Ans. Method used for test tube baby need artificial need artificial collection of sperm & ovum, implantation
in surrogate mother in some occasions which very often discouraged by the society. Couples become
selective & avoid natural process.
Q. Population explosion is the resultant effect of reproductive health awareness. Why?
Ans. Reproductive health awareness resulted in reduced infant mortality rate , discouraging early marriage,
contraceptive devices, control against diseases could increase in population.
Q.What is meant by induced abortion?
Ans. It is an intentional or voluntary termination of pregnancy before full term due to unprotected
intercourse or failure of effect of contraceptive during coitus or rape.
136
CHAPTER 5
Principle of inheritance and Variation:
1 If a dominant allele for tall plants is represented by the letter D, what letter should
represent the corresponding recessive allele?
2 In cats, the allele (S) for short fur is dominant to the allele (s) for long fur.
(a) What is the genotype of a true-breeding, long-furred cat?
(b) What is the phenotype of a cat with the genotype Ss?
(c) In an Ss genotype, which allele is expressed in the phenotype?
(d) Which of the fo1lowing genotypes is (i) heterozygous (ii) homozygous dominant?
SS, Ss, ss
3 In rabbits, assume that the dominant allele (B) produces black fur. The allele (b) for white fur is recessive
to B.
(a) What colour fur will each of the following rabbits have?
Rabbit 1
Rabbit 2
Rabbit 3
genotypeBB
BbbBbb
Rabbit 4
(b) Which of them will breed true?

(c) Which rabbits are homozygous for coat colour?
(d) If rabbits 1 and 4 were mated together and had 12 babies,
how many of these would you expect to be black?
(e) If rabbits 2 and 3 are interbred and produce several litters, totalling 48 babies, how
many white babies would be predicted by the laws of genetics?
(f) If rabbits 3 and 4 are mated together on several occasions and have 50 babies
Altogether, how many of their babies would you 'expect' to be black?
4 The alleles controlling the ABO blood groups are given the letters IA (group A), IB (group B) and i (group
O). On the drawings below, write in the alleles on the chromosomes for each of the blood groups. (clue :
The first one has been done for you)
group A
IA
group B
group AB
IA
or
or
5 Give three examples of human disorders which are caused by the action of a single pair of
alleles. In each case say whether the harmful allele is dominant or recessive to the
non-harmful allele.
137
group O
6 In humans, maleness or femaleness is determined by a pair of sex chromosomes called

X and Y.
(a) What is the genotype for males?
(b) What is the genotype for females?
(c) what are the symbols used for represent male and female genotype in birds?
7 (a) In humans, is it the sperm or the ovum which determines the sex of the offspring?
(b) Give a reason for your answer.
8 When a particular gene is said to be sex-linked, on which chromosome is that gene usually present?
9 colour-blindness is a sex-linked inherited condition controlled by a recessive
allele. Use the symbols X and Y for the sex chromosomes and N and n for the alleles for
normal or defective colour vision to show the genotypes of
(a) a normal male
(d) a colour-blind female
(b) acolour-blind male
(e) a normal (carrier) female.
(c) a normal (non-carrier) female
10 Use the genotypes you have written for your answer to question 9 to show the
Chances of (a) a son being colour blind, (b) a daughter being a carrier, resulting
From a marriage between a normal man and a carrier woman.
11. In a cross between pure tall plants with green pods and a pure dwarf plant with
yellow pods, how many short plants do you expect in F2 generation
a) 9,
b) 3 c) 4
d) 1
12. Is it possible that a male with an extra X chromosome in his genome? If so how it can happen? Mention
its phenotypic characters?
13. a disputed child with blood group O was claimed by two couples; their blood groups are are as
following:
Couple-I
Couple-II
mother father
A
B
O
AB
State with your knowledge of genetics which couple could be the real parent of the child? Also mention
their genotypes.
Note for questions related with pedigree refer study material pg no.54 -58
Few hinds
3. b)-Rabbit 1Rabbit 4
c) Rabbit 1Rabbit 4
d) 12
e) 3:1
f) 50%
138
5.Haemophilia (recessive), albinism (recessive), phenylketonuria (recessive), red-green colourblindness

(recessive), sickle-cell anaemia (partially recessive) (any
three)
11) 4
12. Klinefelterssyndrone.
13. couple I when both are heterozygous
CHAPTER 7
Explain the following:
1.
Biological evolution is the sum total of genetic changes.-Substantiate.
2.
In terms of evolution fittest does not necessarily means strongest.-Explain(the
fittest are not necessarily the strongest individuals, but those individuals who are the bearers of
advantageous inherited traits that allow them to survive and reproduce more than others-natural selection.)
3.
Besides, descent from common ancestor two species can share common
characteristics
.-Explain(due to evolutionary convergence).
4.
Genetic drift affects small populations.-Explain.
5.
The footprint of evolutionary change can be found throughout the naturesubstantiate the statement highlighting predator-prey relationship in terms of natural selection.
(Natural selection favours individuals whose characteristics improve either their ability to consume others or
their ability to avoid being consume.)
6.
Indiscriminant use of antibiotics will jeopardize your future battle against bacteriaJustify.(every time we use antibiotics we are applying selection pressure ,killing off any nonresistant
bacteria thereby , we are actually helping to speed the evolution of resistance to antibiotics)
CHAPTER 8
Human health and disease
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
1) Why do children of metro cities of India suffer from allergies and asthma?
Ans (Hint.-Pollution )
2) A patient has lost his immunity.
i) Name the diseases associated with it.
ii) Name the confirmatoroe test to diagnose the disease.
iii)Why did he lost his immunity.
Ans (Hint:-AIDS)
3)A person claimed that he has seen sounds,heardcolours and smelt light.
i)What could be the possible reason?
ii) Name two chemicles responsible for this conditions.
iii) Mention any one source for these chemicles.
Ans (Hint:- Drug Abuse)
139
CHAPTER 09
Q.1.In mung bean resistance to yellow mosaic vein was developed.(3)
1) Name the phenomenon used.
2) How it is induced?
3) What happens to genes in this method?
HINT:1) Mutation breeding 2)mutations can be induced by chemicals or gamma radiations 3)base
sequences within genes are changed to create variations that results in new characters .
Q.2.What is hidden hunger? what are the defects caused? Name a method of production of improved
quality food that can minimize/prevent it.(3)
Hint: 3billion people suffer from micronutrient,protein and vitamin deficiencies.
Increases risk of diseases, reduces lifespan & mental abilities.
Biofortification.
Q.3.Conventional agriculture is not able to meet demand of food for ever increasing population.SCP can
serve as an alternate.Justify.(3)
Hint: Spirulina & Methylophilus methlotrophus.
Q.4.a)Following are some statements arrange them in sequence beginning from the first step(2)
1.Transferred to a surrogate mother.
2.It is either mated with an elite bull or artificially inseminated.
3.Fertilized eggs at 32 cell stage are recovered non surgically.
4.It produces 6 to 8 eggs instead of one egg which they normally yield per cycle.
b)These steps are of which method of animal breeding?(1)
Q.5.Animal protein can be used extensively for feeding growing population.However nothing much has
been done in this area.Suggest some alternative ways how animal proteins can be obtained on alarge
scale cost effectively?(5)
CHAPTER10
Q.1.How is sewage subjected to various treatments in sewage treatment plant? (3)
Hint: explaination of primary and secondary treatment.
Q.2. Biofertilisers are preferred over chemical fertilizers - Substantiate ?
Hint: Mention the eco friendly sustainable use of biofertilisers.
Q.3.Three water samples labeled A (river water); B(untreated sewage water) and C(secondary
effluent)were taken for BOD test. The BOD values were 20mg/l; 8mg/l &400mg/l, respectively. Which water
sample is most polluted? Assign the correct label to each assuming the river water is relatively clean? (3)
Hint: sample A BOD 20mg/l; sample B BOD 8mg/l &sample C 400mg/l
Q.4.A) Who gave the medicinal importance of Antibiotics? (1)
B) Give the functional importance of Propionibacterium. (1)
c) Name the bacterium that produces the insecticidal cry protein? (1)
Q.5.Supply the scientific terms for the following (5)
140
1) The waste and waste water produced by residential and commercial sources and discharged into
sewers.
2) An approach to farming based on biological methods that avoid the use of synthetic crop/livestock
production inputs.
3) A group of gram positive bacteria that carry out lactic acid fermentation of sugars.
4) The sludge produced by primary treatment in a wastewater treatment plant.
5) A systems approach that combines a wide array of crop production practices with careful monitoring of
pests and their natural enemies.
Hint: a)sewage b)organic farming c)LAB d)primary sludge e)IPM
Q.6.why should biological control of pests and pathogens be proffered to the conventional use of chemical
pesticides? Explain how the following microbes act as biocontrol agents:(1+1+1)
a)Bacillus thuringiensis b)Nucleopolyhedroviruses
Q.7. During secondary treatment of the primary effluent how does the significant decrease in BOD occur?
(3)
Q.8a) Draw a labelled diagram of a typical biogas plant.

hint:5) floating gas holder 3)sludge 9) digester 1) Dung and water
b) Give the role of spent slurry
Q.9 Explain Why :
(a) Cow dung is used in the generation of biogas.
(b) A small amount of curd is added in fresh milk to convert it into curd.
(c) Baculovirus are used in narrow spectrum insecticidal application.
Q.10 What are antibiotics? Give two examples. What is their significance?(3)
Q.11. Give one example and one use of the following: a) Free living fungi b) Symbiotic fungi c) Free living
bacteria (3)
Q12) How is it that the Cry protein produced by Bacillus thuringiensis (Bt) does harm the bacteria but only
killsthe insect larvae? (3)
5 Marks Questions
Q1)Explain how microbes are used in sewage treatment?
141
Q2)What do you understand by integrated pest management (IPM)? Explain with example and state its
importance
Q5) Differentiate Antibodies and antibiotics Q6) How are biofertilizers different from fertilizers such as NPK that we
buy in the market? Justify the role of Rhizobium as a biofertilizer
CHAPTER 11
1) Why dont restriction enzyme digest chromosomal DNA in bacterial cells ?
2) Why do bacteria have plasmids?
3) Why thermostable DNA polymerase is essential in PCR?
4)Eukaryotes do not have restriction endonuclease, then how they manage with normal endonuclease
enzyme?
5) It is advisable to use different restriction endonucleases to cut the vector DNA and source DNA.Why ?
6) Uncontrolled recombinant DNA technology experiments is dangerous to mankind. Comment on it. 167
4) Foreign DNA + plasmid =??
5 ). Complete the above sequence of diagrammatic representation and name it.
(a) Which is the most commonly used matrix in gel electrophoresis ?
(b) What is the source of it?
6) Find the odd one out and write why that is odd
(a) Sal I, Pst I, Cla I, BamH I, pBR 322
(b) Bacteria, Virus, Gene-gun, Fungi
7) Detect the mismatch from the following and replace the wrong match with a right one
(a) ECOR I Bacteria
(b) Ethidium Bromide- Gel electrophoresis
(c) Lysozyme- Fungi
(d) Palindrome sequence-Restriction enzyme
8). Name the enzyme involved in the following process:
(a) Repeated amplification of DNA fragments.
(b) Formation of short piece of RNA strand for annealing.
(c) Breaking of bacterial cell to release DNA and other macromolecules.
(d) Cutting and rejoining DNA fragments.
(e) Formation of m-RNA
(f) Joining of foreign DNA fragments with plasmid.
9)Explain how recombinants and non- recombinants are differentiated on the basis of colour production in
the presence of a chromogenic substrate. Name that procedure.
142
Have some more

1. When scientist make an animal superior by view of genotype, introducing some foreign
gene in it , the phenomenon is called ______________.
2. Why DNA is unable to pass through cell membrane?
3. Why dont the restriction enzymes destroy the DNA of the organism in which they are
produced?
4. What function the enzymes DNA ligase perform in genetic engineering?
5. What are the essential features of a vector?
6. Which property of plasmid makes them ideal vectors for gene cloning
7. Discuss the use of molecular probes in forensic science for identification of criminals.
8. What is vector less gene transfer? What are the methods used to transfer genes directly in
plants?
9. Name two bacterias found to be very useful in genetic engineering?
10. Agrobacterium tumefaciens is known as natural genetic engineer of plants why?
11. What do you understand by insertion inactivation genetic engineering? State its usefulness
12. What is the significance of ori-gene (origin of replication) in a plasmid?
13. Name the substance used to stain DNA fragments separated in gel electrophoresis? How
they are visualized
Few clues
5. refer concept map
6.Self replicating
8.gene gun, microinjection etc
9.Escherichia coli ,Agrobacterium tumefaciens
10 the can cause Crown Gall disease by transferring Ti plasmid to higher plants naturally
13 Ethidium bromide/UV light
CHAPTER12:
1.Gene medicine refers to the use of gene manipulation technology to ameliorate or even permanently cure
disease in human-Name the technique.(Gene therapy)
2. Agrobacterium tumefaciens are considered as natural genetic engineer.-Justify.
3.The bacterium Bacillus thuringiensis provides the major source of insect resistant gene-clarify.
4. 'RNAsilencing is a form of genomic defense'-elucidate the statement taking M. incognitia as example.
143
CHAPTER13:
Organism,population
1.When two species of Paramoecium(P. caudatum and P. aurelia were grown together in the laboratory, at
first both the species grow in number,eventually however, P. caudatum declines in number while P. aurelia
continues to increase in number.-Which type of animal interaction can substantiate the above
phenomenon?(competition)
2.Plants like beech, Oak and Pine gain amino acids from fungal associationship , while the fungi in return
receive carbohydrates and vitamins from the tree-What type of interaction can be inferred from
this?(Mutualism)
3.In the stomach of ruminants huge number of Cellulolytic bacteria are present which help the herbivores to
digest the plant material, in turn, the bacteria receive Nitrogen that has been secreted or ingested into the
rumen in the form of urea-Name the type of interaction.
CHAPTER 14
1 Why is dry weight chosen for expressing the the biomass of a species ?
Hint To avoid variation in weight due to seasonal moisture difference
2 In a pyramid of biomass drawn below name themembers of each trophic levels
1one which issupported
2the one which supports.
In which ecosystem such apyramid is found?
Ans 1zooplankton
2 phytoplankton
Aquatic ecosystem
3Explain why ecological succession will be faster in forest devastated by fire than on bare rock?Also
compare succession in case of an abandoned land after floods with that on a bare rock?
144
CHAPTER 15
Explain Why ?
1. Indonesia exhibits more biological diversity than Polland. (Mention why there is more biodiversity
in tropics)
2. National Parks is a better option for the conservation of biodiversity than zoological
gardens.(Mention about the advantages of in-situ conservation over ex-situ conservation).
3. Loss of Key stone species from an ecosystem will be a major threat to ecosystem function.(Give
your answer on the basis of rivet popper hypothesis.)
4. India is a megadiversity country.
5. Within your sibling there are lots of variation although, all of you have the same parents(answer on
the basis of genetic diversity).
6. India nurtures a lot greater biodiversity than Norway.(answer the question based on greater
ecosystem diversity in India than in Norway).
7. Justify the killing of elephants at North Bengal in the light of biodiversity conservation.(Habitat
loss/fragmentation/shrinkage by the construction of rail line through the elephant corridor in North
Bengal leads to man animal conflict and resultant loss of biodiversity).
8. The unique mangrove biodiversity of Sunderbans will totally wiped away (Frame your answer on
the basis of global climate change and subsequent biodiversity loss)
9. Find the odd one out:
Eicchorniasp., Lantana sp. ,Partheniumsp. , Oryza sativa.(Besides, Oryza sativa all other are
invasive species)
CHAPTER 16
1) What is the norms set by Euro-II for petrol and Diesel vehicles
2) Name the Phenomena Which Keeps the Earth Warmer than Moon.
3) Name the important ozone depleting substances.
4) Why is thermal pollution harmful for aquatic life?
5) Why are cloudy, dusty & humid Nights warmer than clear dust free and dry nights?
6)(Fig 16.3 pg No. 274-biology text book for class xii)
In the above graph what does A & B depict?
145
LIST OF ABBREVIATIONS AND THEIR EXPANSION

Chapter2:
MMC: Megaspore Mother Cell
PEC: Primary Endosperm Cell
Chapter3:
LH: Luteinising Hormone
FSH: Follicle Stimulating Hormone
hpl : Human placental lactogen
hcg: human chorionic gonadotropin
Chapter4:
STDs; Sexually Transmitted Diseases
RCH: Reproductive and Child Health Care
MMR: Maternal Mortality Rate
IMR: Infant Mortality Rate
IUDs: Intra Uterine Devices
MTP: Medical Termination of Pregnancy
VD: Venereal Diseases
RTI: Reproductive Tract Infection
HIV: Human Immunodeficiency virus
PID: Pelvic Inflammatory Diseases
ART: Assisted Reproductive Technologies
IVF: In vitro Fertilization
ZIFT: Zygote intra fallopian transfer
IUT: Intra Uterine transfer
GIFT: Gamete intra fallopian transfer
ICSI: Intra Cytoplasmic Sperm Injection
AI: Artificial Insemination
IUI: Intra uterine insemination
Chapter6:
sn RNAs: small nuclear RNAs
hn RNAs: heterogenous nuclear RNA
HPG: Human Genome Project
ESTs: Expressed Sequence Tags
BAC: Bacterial Artificial Chromosomes
YAC: Yeast Artificial Chromosomes
SNPs: Single Nucleotide Polymorphism
VNTR: Variable Number of Tandem Repeats
146
Chapter8:
PMNL: Polymorpho nuclear leucocytes
CMI: Cell mediated immunity
MALT: Mucosal Associated Lymphoid Tissue
AIDS: Acquired Immuno Deficiency Syndrome
ELISA: Enzyme Linked Immuno-sorbent Assay
NACO: National AIDS Control Organisation
MRI: Magnetic Resonance Imaging
CT: Computed Tomography
Chapter9:
SCP: Single Cell Protein
Chapter10:
LAB: Lactic Acid Bacteria
BOD: Biochemical/Biological Oxygen Demand
Chapter11:
PCR: Polymerase Chain Reaction
Chapter12:
RNAi: RNA interference
GMO: Genetically Modified Organism
ADA: Adenosine deaminase deficiency
GEAC: Genetic Engineering Approval Committee
GPP: Gross Primary PRODUCTIVITY
NPP: Net Primary ProductivityGFC: Grazing Food Chain
DFC: Detritus Food Chain
DU: Dobson unitCFCs: Chloroflurocarbons
JFM: Joint Forest Management
147

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BIOLOGY

Kendriya Vidyalaya Sangathan

MEMBERS OF THE CONTRIBUTING TEAM

PGT BIOLOGY -K.V. No.1 BINAGURI

2. Mrs. SOMA BANERJEE

PGT BIOLOGY-K.V. BURDWAN

3.Mrs. RUBY .C. DESHMUKH

PGT BIOLOGY-K.V. SALUA

4. Dr. SABYASACHI MAJUMDAR

PGT BIOLOGY-K.V. MALDA

5.Mr GAUTAM BISWAS

PGT BIOLOGY-K.V. BARRACKPORE (ARMY)

PGT BIOLOGY-K.V. BAMANGACHI

7.Mrs. LIPIKA DEY

PGT BIOLOGY-K.V. No.1 KANCHRAPARA

8.Mrs. G. VIJAY LAKSHMI

PGT BIOLOGY-K.V. BARRACKPORE (AFS)

Ms. CHANDANA MANDAL

Biodiversity and conservation

Chapter 1: - REPRODUCTION IN ORGANISMS

Every organism live only for a certain period of time.

External fertilization zygote formed in external medium water eg. Frog,

Parthenogenesis: Female gamete develops into new organism.

Question & Answer

CHAPTER 2 SEXUAL REPRODUCTION INFLOWERING PLANTS

Four WHORLS CALYX (sepals), COROLLA (petals), ANDROECIUM (Male

Male Reproductive Organ

Pollen grains have two outer walls; i) Exine ii) Intine

GYNOECIUM / CARPEL (THE FEMALE REPRODUUCTIVE ORGAN)

Cross pollination (Hetrogamy)

Xenogamy (different flowers on

Pollen grains germinate on stigma

SYNGAMY: Fusion of one male gamete(n) with egg (n)

Refer fig 2.13 in NCERT

POST FERTILISATION CHANGES:

Chapter-3 HUMAN REPRODUCTION

The Male reproductive system

a. Located behind penis

Stores sperm until they have matured.

Tube that leads from the epididymis to the urethra.

Produce food for sperm. Food "Fructose"

Produces clear lubricating fluid

The Female Reproductive System

Differentiate between: Spermatogenesis and oogenesis :

Produces male gametes (sperm)

produces female gametes (oocytes)

occurs in the seminiferous tubules (in testes)

occurs in the ovaries

may produce 400,000,000 per day

humans normally produce one oocyte during

Primary spermatocyte divide equally to form two

Primary otocyte divide unequally to form one

One spermatogonium produces 4 functional

An oogonium produces one functional ovum and

2)Follicular phase (Proliferative phase ) and Luteal phase (Secretory Phase)

Stage of repair and proliferation

Prepares endometrium for implantation

It extends from the end of menstruation to ovulation

It extends after ovulation to menstruation