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05 Spectros

The document discusses spectroscopy techniques and provides examples of analyzing samples using spectroscopy. It includes 10 problems analyzing samples, calculating percentages of elements, determining molecular structures from NMR spectra, and identifying isotopes based on infrared spectroscopy data.

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Jihee Yoon
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123 views2 pages

05 Spectros

The document discusses spectroscopy techniques and provides examples of analyzing samples using spectroscopy. It includes 10 problems analyzing samples, calculating percentages of elements, determining molecular structures from NMR spectra, and identifying isotopes based on infrared spectroscopy data.

Uploaded by

Jihee Yoon
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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GENERAL CHEMISTRY WORKSHOP

SPECTROSCOPY
1. A sample of steel from a bicycle frame is to be analyzed to determine its manganese
content. A sample of the steel weighing 0.1523 g was dissolved, and the unknown
-1
amount of manganese was converted to MnO4 . Water was then added to give a
solution with a final volume of 100.0 mL. A portion of this solution was placed in a
spectrophotometer, and its absorbance was found to be 0.780 in a 1.00 cm cuvette.
Given that for MnO4-1 in aqueous solution is 2.48 x 103 L/mol cm, calculate the
percent manganese in the steel.
2. The molar extinction coefficient of lysozyme at a pH of 6.1, for an incident frequency
of 1645 cm-1, is 2.0 x 104 L mol-1 cm-1. An aqueous solution containing 80. mg/mL of
this enzyme has an 8.3% transmittance at 1645 cm-1 in a cuvette of 0.100 mm light
path. What is the approximate molar mass of lisozyme?
3. The absorption of ultraviolet light of wavelength 280 nm by proteins is caused almost
entirely by the aromatic amino acids tryptophan and tyrosine. Suppose that a protein
of molar mass of approximately 26,000 amu contains two residues of tryptophane (try
= 5.0 x 103 L mol-1 cm-1) and six residues of tyrosine (tyr = 1.1 x 103 L mol-1 cm-1).
The absorbance is recorded from a cuvette of 1.00 cm path length, holding the protein
concentration at 1.00 mg/mL. Calculate the absorbance and the percent transmittance
of this solution at 280 nm.
4. Absorption by H35Cl occurs near 200. cm-1, and the spacing between neighboring
lines is 20.89 cm-1. Find the moment of inertia and the internuclear distance in H35Cl.
5. The infrared spectrum of X16O shows the = 0 to = 1 transition at 1877 cm-1. If the
force constant of X16O is 1550. N m-1 and the atomic mass of 16O is 15.9995 amu,
determine the identity of the isotope X.
6. Draw the idealized NMR spectra for the following compounds: a) CH2Cl2, b) CH3-CHI2,
c) CH2F-CO-CHBr2, d) CH3 -O- CH3, e) CH2=CBr-CH3.
7. Draw the idealized NMR spectra for the following compounds. (Assume that the five
hydrogen atoms in the benzene are equivalent with no spin-spin coupling)

8. Draw the idealized NMR spectra for the following compounds: a) bromoethane,
b) 2-butanone, c) ethylmethyl ether, d) toluene, (Assume that the five hydrogen atoms in
the benzene are equivalent with no spin-spin coupling), e) methyl acetate.
9.

Draw the idealized NMR spectra for the following compounds: a) CH3CH2CH2Br,
b) CH2=CHCH2CH3, c) CH3CH2-O-CH2CHFCH3.

10. The NMR spectra below are for the alkene C6H12 and for the alkane C4H10O. Deduce
the structures for these compounds. Please notice that the TMS reference has been
omitted in each spectrum.

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