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Evaporative Light Scattering Detector

The document discusses various liquid chromatography detectors, focusing on the evaporative light scattering detector (ELSD). It describes the ELSD's principle of droplet formation, solvent evaporation, and light scattering detection. The document also discusses ELSD instrumentation, optimization, and its application to analyzing cholesterol and other compounds. It finds the ELSD provides a fast, stable, and reproducible technique with no baseline shifts from solvent changes and a low method detection limit.

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1K views13 pages

Evaporative Light Scattering Detector

The document discusses various liquid chromatography detectors, focusing on the evaporative light scattering detector (ELSD). It describes the ELSD's principle of droplet formation, solvent evaporation, and light scattering detection. The document also discusses ELSD instrumentation, optimization, and its application to analyzing cholesterol and other compounds. It finds the ELSD provides a fast, stable, and reproducible technique with no baseline shifts from solvent changes and a low method detection limit.

Uploaded by

frankkuba
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Franklin Ifeanyichukwu Uba

CHEM 7750 - Separation science


LC detectors

• Refractive index Detector (RID)


• Fluorescence detector

• Evaporative light
scattering detector (ELSD)

• UV-Detector
• Electrolytic conductivity detector • Electrochemical detectors
http://hplc.chem.shu.edu/HPLC/index.html
ELSD principle
• Elution
• Droplet formation
• Solvent Evaporation
• Light scattering
(Rayleigh)
• Detection at 45O
• Mass detector
R.P.W.Scott, Chrom-Ed Book series, Book 5, 2003.
Rayleigh scattering
• Elastic collision

ɵ
High energy Particle
photon

ɵ = Scattering angle http://upload.wikimedia.org/wikipedia/en/6/69/Rayleigh_sunlight_scattering.png

• KE conservation
• Particles smaller than wavelength of light
ELSD Instrumentation

http://www.sielc.com/images/ELSD_Schematic.gif
http://www.chem.agilent.com/
Cholesterol and COPs

http://www.scielo.cl/fbpe/img/bres/v36n3-4/fig01.gif
Previous methods
• Thin Layer Chromatography
• Qualitative
• Loading capacity restrictions
• Prone to contamination
• Gas Chromatography
• High temperatures (inlet/oven)
• Volatile derivatives (quantitative efficiency)
• High Pressure Liquid Chromatography
• No derivatives
• Faster
• Better accuracy than GC and TLC
Elution conditions
• Normal phase column – 250 mm x 46 mm i.d.
• 16 % Alumina / 84% Silica ( 5μm )
• Guard Column – 20 mm x 2 mm

• Gradient elution – 1.0 mL/min


• Dichloromethane (A) / Acetonitrile : Water (99.05:0.95) (B)
• 94:6 / A:B for 0 – 6 min
• 79:21/ A:B for 27 min
• Linear Gradient to 94:6 for 5 min

• ELSD detector : 75 OC; N2 1.5 L/min


ELSD optimization
Results
Linearity
Area = k (conc.)m

m = coefficient of curvature in
conc./peak area curves

ln(area) = ln K + m ln(conc.)

Normalized peak areas versus


concentration (0.1 – 1.0 g)
Conclusions

• Fast analytical technique


• MDL of 75 ng/injection
• Stable and reproducible detector
• No baseline shift due to solvent changes
Acknowledgement
• Dr Gilman

• CHEM 7750 Classmates


Hashim Treyvon Danielle

Jon Akanksha Nyote

Suzana Paul Sung

Kumuditha Sudha

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