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Universal Primers

The document lists over 80 universal primer sequences that are commonly used for applications like PCR amplification, sequencing, and cloning. The primers target various genes, vectors, and genetic elements. Each primer is listed along with its name and 5' to 3' nucleotide sequence.

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Siraj
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149 views3 pages

Universal Primers

The document lists over 80 universal primer sequences that are commonly used for applications like PCR amplification, sequencing, and cloning. The primers target various genes, vectors, and genetic elements. Each primer is listed along with its name and 5' to 3' nucleotide sequence.

Uploaded by

Siraj
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Universal Primers

1492R

Sequence (5' - 3')


5' TACGGYTACCTTGTTACGACTT 3'

27F

5' AGAGTTTGATCMTGGCTCAG 3'

35S-A

5' AAGGGTCTTGCGAAGGATAG 3'

35S-B

5' AGTGGAAAAGGAAGGTGGCT 3'

518F

5' CCAGCAGCCGCGGTAATACG 3'

800R

5' TACCAGGGTATCTAATCC 3'

AD Reverse

5' AGATGGTGCACGATGCACAG 3'

a-Factor

5' TACTATTGCCAGCATTGCTGC 3'

AOX1 Forward

5' GACTGGTTCCAATTGACAAGC 3'

AOX1 Reverse

5' GCAAATGGCATTCTGACATCC 3'

BGH-R

5' TAGAAGGCACAGTCGAGG 3'

Bluescript KS

5' TCGAGGTCGACGGTATC 3'

Bluescript SK

5' CGCTCTAGAACTAGTGGATC 3'

CMV-F

5' CGCAAATGGGCGGTAGGCGTG 3'

CYC1 Reverse

5' GCGTGAATGTAAGCGTGAC 3'

DsRed1-C

5' AGCTGGACATCACCTCCCACAACG 3'

DsRed1-N

5' GTACTGGAACTGGGGGGACAG 3'

EBV-RP

5' GTGGTTTGTCCAAACTCATC 3'

EGFP-C

5' CATGGTCCTGCTGGAGTTCGTG 3'

EGFP-CF

5' AGCACCCAGTCCGCCCTGAGC 3'

EGFP-CR

5' CGTCCATGCCGAGAGTG 3'

EGFP-N

5' CGTCGCCGTCCAGCTCGACCAG 3'

EGFP-NR

5' CGTCGCCGTCCAGCTC 3'

GAL1 Forward

5' AATATACCTCTATACTTTAACGTC 3'

Gal4AD

5' TACCACTACAATGGATG 3'

GLprimer1

5' TGTATCTTATGGTACTGTAACTG 3'

GLprimer2

5' CTTTATGTTTTTGGCGTCTTCCA 3'

HCO2198

5' TAAACTTCAGGGTGACCAAAAAATCA 3'

ITS1

5' TCCGTAGGTGAACCTGCGG 3'

ITS2

5' GCTGCGTTCTTCATCGATGC 3'

ITS3

5' GCATCGATGAAGAACGCAGC 3'

ITS4

5' TCCTCCGCTTATTGATATGC 3'

ITS5

5' GGAAGTAAAAGTCGTAACAAGG 3'

KAN2-FP

5' ACCTACAACAAAGCTCTCATCAACC 3'

KAN2-RP

5' GCAATGTAACATCAGAGATTTTGAG 3'

LCO1490

5' GGTCAACAAATCATAAAGATATTGG 3'

M13F (-20)

5' GTAAAACGACGGCCAGT 3'

M13F (-29)

5' CACGACGTTGTAAAACGAC 3'

M13-FP

5' TGTAAAACGACGGCCAGT 3'

M13F-pUC(-40)

5' GTTTTCCCAGTCACGAC 3'

Universal Primers
M13R (-20)

Sequence (5' - 3')


5' GCGGATAACAATTTCACACAGG 3'

M13R (-24)

5' GGAAACAGCTATGACCATG 3'

M13R-pUC (-26)

5' CAGGAAACAGCTATGAC 3'

MT Forward

5' CATCTCAGTGCAACTAAA 3'

pBacPAC-RP

5' GTCTGTAAATCAACAACGC 3'

pBAD-F

5' ATGCCATAGCATTTTTATCCA 3'

pBAD-FP

5' ATGCCATAGCATTTTTATCC 3'

pBAD-R

5' GATTTAATCTGTATCAGG 3'

pBRrevBam

5' GGTGATGTCGGCGATATAGG 3'

pDONOR-FP

5' TAACGCTAGCATGGATCTC 3'

pEGFP_N

5' CCGTCCAGCTCGACCAG 3'

pEGFP-FP

5' TTTAGTGAACCGTCAGATC 3'

pEGFP-RP

5' AACAGCTCCTCGCCCTTG 3'

pESP-RP

5' TCCAAAAGAAGTCGAGTGG 3'

pET-24a

5' GGGTTATGCTAGTTATTGCTCAG 3'

pET-RP

5' CTAGTTATTGCTCAGCGG 3'

pFastBac Forward

5' GGATTATTCATACCGTCCCA 3'

pFastBac Reverse

5' CAAATGTGGTATGGCTGATT 3'

pGEX3

5' GGAGCTGCATGTGTCAGAGG 3'

pGEX5

5' GGCAAGCCACGTTTGGTG 3'

pJET1.2F

5' CGACTCACTATAGGGAGAGCGGC 3'

pJET1.2R

5' AAGAACATCGATTTTCCATGGCAG 3'

pMalE

5' TCAGACTGTCGATGAAGC 3'

pQE-F

5' CCCGAAAAGTGCCACCTG 3'

pQE-R

5' GTTCTGAGGTCATTACTGG 3'

pREP-fwd

5' GCTCGATACAATAAACGCC 3'

pRH Forward

5' CTGTCTCTATACTCCCCTATAG 3'

pRH Reverse

5' CAAAATTCAATAGTTACTATCGC 3'

pTrcHis Forward

5' GAGGTATATATTAATGTATCG 3'

QE Promoter

5' CCGAAAAGTGCCACCTG 3'

RVprimer3

5' CTAGCAAAATAGGCTGTCCC 3'

RVprimer4

5' GACGATAGTCATGCCCCGCG 3'

SP6

5' ATTTAGGTGACACTATAG 3'

STag 18mer Primer

5' GAACGCCAGCACATGGAC 3'

SV40-pArev

5' CCTCTACAAATGTGGTATGG 3'

SV40-Promoter

5' GCCCCTAACTCCGCCCATCC 3'

T3

5' ATTAACCCTCACTAAAG 3'

T7

5' AATACGACTCACTATAG 3'

T7 EEV

5' ATGTCGTAATAACCCCGCCCCG 3'

Universal Primers
T7promoter

Sequence (5' - 3')


5' TAATACGACTCACTATAGGG 3'

T7terminator

5' GCTAGTTATTGCTCAGCGG 3'

U-19mer Primer

5' GTTTTCCCAGTCACGACGT 3'

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