The standard operating procedure outlines the steps for determining hematocrit, which is the volume of packed red blood cells (RBCs) in a given volume of whole blood. Key steps include filling capillary tubes three-quarters full with anticoagulated blood, sealing the tubes, centrifuging them to separate components, and reading the packed RBC level without including the buffy coat using a microhematocrit reader. The procedure should be followed carefully to mix specimens properly, seal tubes completely, and centrifuge at the appropriate speed and time to obtain an accurate hematocrit measurement within 1% between duplicate tests.
The standard operating procedure outlines the steps for determining hematocrit, which is the volume of packed red blood cells (RBCs) in a given volume of whole blood. Key steps include filling capillary tubes three-quarters full with anticoagulated blood, sealing the tubes, centrifuging them to separate components, and reading the packed RBC level without including the buffy coat using a microhematocrit reader. The procedure should be followed carefully to mix specimens properly, seal tubes completely, and centrifuge at the appropriate speed and time to obtain an accurate hematocrit measurement within 1% between duplicate tests.
The standard operating procedure outlines the steps for determining hematocrit, which is the volume of packed red blood cells (RBCs) in a given volume of whole blood. Key steps include filling capillary tubes three-quarters full with anticoagulated blood, sealing the tubes, centrifuging them to separate components, and reading the packed RBC level without including the buffy coat using a microhematocrit reader. The procedure should be followed carefully to mix specimens properly, seal tubes completely, and centrifuge at the appropriate speed and time to obtain an accurate hematocrit measurement within 1% between duplicate tests.
The standard operating procedure outlines the steps for determining hematocrit, which is the volume of packed red blood cells (RBCs) in a given volume of whole blood. Key steps include filling capillary tubes three-quarters full with anticoagulated blood, sealing the tubes, centrifuging them to separate components, and reading the packed RBC level without including the buffy coat using a microhematocrit reader. The procedure should be followed carefully to mix specimens properly, seal tubes completely, and centrifuge at the appropriate speed and time to obtain an accurate hematocrit measurement within 1% between duplicate tests.
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STANDARD OPERATING PROCEDURE: HEMATOCRIT DETERMINATION
1. INTRODUCTION The hematocrit is the volume of packed RBC that occupies a given volume of whole blood. This is often referred to as the packed cell volume (PCV). It is reported either as a percentage or in liters per liter. 2. POLICY 2.1. Do not include buffy coat hen reading (leukocyte and platelets). 2.2. Properly seal the capillary tube to reduce decreased hematocrit reading as a result of loss of blood during centrifugation. 2.3. Mixed the specimen properly. 3. MATERIALS AND EQUIPMENT 3.1. 3.2. 3.3. 3.4.
4. PROCEDURE 4.1. Fill two plain capillary tubes approximately three quarters full with blood anticoagulated with EDTA or heparin. Any excess blood from the outside of the tube. 4.2. Seal the end of the tube with the colored ring using nonabsorbent clay. Hold the filled tube horizontally and seal by placing the dry end into the tray with sealing compound at a 90-degree angle. 4.3. Rotate the tube slightly and remove it from the tray. The plug should be at least 4 mm long. 4.4. Balance the tubes in the centrifuge with the clay ends facing the outside away from the center, touching the rubber gasket. 4.5. Tighten the head cover on the centrifuge and close the top. 4.6. Centrifuge the tubes at between 10,000 g and 15,000 g for the time that has been determined to obtain maximum packing of RBCs. 4.7. Determine the hematocrit by using a microhematocrit reading device. Read the level of RBC packing. 4.8. The values of the duplicate hematocrits should agree within 1% (0.01 L/L). 5. REFERENCE RANGE
