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Tricho Egg Card Containing

1. The document describes a process for parasitizing eggs of the parasitoid Trichogramma with a 10% gum arabic solution. Eggs are sprinkled uniformly on card grids and excess eggs are removed with a brush after drying. 2. Parasitized eggs are placed in bags with nucleus cards containing ready-to-emerge Trichogramma adults to further parasitize the fresh eggs. The parasitoid to host ratio is adjusted to 1:6 for effective parasitism. 3. Parasitized eggs turn black within 3-4 days and parasitoids emerge within 8-10 days, and can be stored in refrigeration for up to 3 weeks without loss of emergence

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Ananda Preethi
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49 views3 pages

Tricho Egg Card Containing

1. The document describes a process for parasitizing eggs of the parasitoid Trichogramma with a 10% gum arabic solution. Eggs are sprinkled uniformly on card grids and excess eggs are removed with a brush after drying. 2. Parasitized eggs are placed in bags with nucleus cards containing ready-to-emerge Trichogramma adults to further parasitize the fresh eggs. The parasitoid to host ratio is adjusted to 1:6 for effective parasitism. 3. Parasitized eggs turn black within 3-4 days and parasitoids emerge within 8-10 days, and can be stored in refrigeration for up to 3 weeks without loss of emergence

Uploaded by

Ananda Preethi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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The cost of 10% gum Arabic is applied

on the grids (G1-30)and the eggs are


sprinkled uniformly in a single layer
with the aid of a tea strainer. The
excess eggs pasted removed by
gently passing a shoe brush over the
card after sufficient air drying under
fan. The egg cards are placed into
polythene bags of suitable size and
the nucleus card of trichogramma are
introduced in it. The earliest way to
accomplish this is to place a piece of
tricho egg card containing
parasitized eggs that are ready to
yield the adults and to hold them in
subdued light for 2 to 3 days. the
emerging parasites readily parasitize
the fresh eggs. The parasitoid host
ratio is adjusted accordingly to 1:6
get effective parasitism. The

parasitized eggs in the tricho card turn


to black in 3 or 4 days and the adult
parasitoids emerge in 8 to 1o days
from the date of parasitization. The
parasitized eggs in which the
parasitoids in the larval or pupal
stage can be stored in the refrigerator
for about 3 weeks without any loss in
emergence.
Pracautions:
Poor quality of mass reared
trichogrmma can result in control
failures. The artificial conditions of
mass rearing can select for genetic
changes that reduce the effectiveness
of the trichogrmma in the field. Such
rearing conditions include rearing
multiple generations on unnatural
host eggs, the absence of plants ,

crowding and interference ,rapid


generation time ,and failure to
rejuvenate genetic stock. Except for
obvious problems such as lace of adult
emergence or wing deformities ,
growers and pest consultants cannot
detect poor quality trichogrmma prior
to release. Commercial suppliers are
responsible for maintaining desirable
characteristics necessary for good
performance in the field. Production
colonies should be periodically
replaced with individuals from a stock
culture maintained on the natural or
target host.

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