Genotype Phenotype Lab: Overview
Genotype Phenotype Lab: Overview
Overview:
Duringclasstodaystudentswilllearnabouthumangenotypesandphenotypesby
participatinginalabdesignedtoshowhowtraitsareexpressedviagenes.Theywillself
documentseveraloftheirphenotypesaswellastheirpossiblegenotypes.Wewillthencompile
aclasscharttoshowhowdifferentproportionsofphenotypesinapopulationshowgeneticists
ifanalleleisdominant,recessive,orcodominant.Theywillthenbegiventimetoanswer
questionsaskedattheendofthelab.
Objectives:
Studentswillunderstandbetterhowtheirphysicaltraitsaregovernedbytheirgenes.
Studentswillknowtheirgeneticmakeupforseveraltraits.
Studentswillseehowdifferentratiosrepresentedinapopulationscumulative
phenotypeshowgeneticistswhetherthetraitisdominant,recessive,orcodominant.
Studentswillbeabletoabletodistinguishbetweendominantandrecessivealleles.
Studentswillbeabletodeterminethephenotypethatresultsfromagivengenotype.
Standards:
LS.12Recognizethattraitscanbestructural,physiologicalorbehavioralandcaninclude
readilyobservablecharacteristicsattheorganismallevelorlessrecognizablefeaturesatthe
molecularandcellularlevel.
LS.12Distinguishbetweendominantandrecessiveallelesanddeterminethephenotypethat
wouldresultfromthedifferentpossiblecombinationsofallelesinanoffspring.
LS.12Describedominant,recessive,codominant,sexlinked,incompletelydominantand
recessivealleles,punnettsquares,traits,andillustratetheirinheritancepatternsovermultiple
generations.
Materials:
Studentanswersheet,labguidesheet,whiteboard,PTCpaper.
Lesson:
LabIntroduction[5min]
oFirst,Iwillexplainhowthelabwillworkforthestudents,andhowitrelatesto
humangeneticsandtopicscoveredinpreviouschapters.WhiledoingthisIwill
handoutthePTCtastestrips.
oPhenotypesareourtraitsandtheexpressionofourgenesorourgenotypes.
oDominantalleleswillexpresstheirtraitsandmaskarecessivealleleiftheyare
pairedtogether.
oIttakestworecessiveallelesfortherecessivetraittobeexpressed.
oWecanlookatyourphysicaltraitsanddeterminewhetherthedominantor
recessivetraitisbeingexpressed.Ifthedominanttraitisshownweknowyou
haveoneoftwogenotypes.Iftherecessiveconditionisshown,weknowyou
mustbehomozygousrecessiveforthetrait.
PTCtastingtrait[10min]
oOnceIvehandedoutthePTCpaperIwillexplainthatifthestudentstaste
somethingsouronthepapertheyarepositiveforPTCtastinggeneandiftheytaste
nothingtheyarenegativeforthegene.
oIwillthenshowthemhowtofilloutthechartontheiranswersheetusingthePTC
tastinggeneasanexample.
GenotypePhenotypeLabworktime[15min]
oNextIwillhavethemgetintopartnerstorunthroughthelabwhichincludesseveral
othertraits.
oTheywillrecordtheirfindingsonachartincludedintheiranswersheet.
oAftertheyfinishthechartontheiranswersheetIwillhaveeachgroupputtheir
findingsonaclasschartdrawnonthewhiteboard.
oTheywillthenhavetimetoanswertherestofthequestionsaskedofthemintheir
lab.
Inheritancepatterns[5min]
oOnceeverystudenthasenteredtheirinformationintotheclasschartIwilltotal
theirnumbers.
oThen,Iwillgetthestudentsattentionandshowtheclasstotalsforeachtrait.
oUsingguidingquestions,Iwillthendemonstratethatbecausesometraitsare
recessivetheyarerepresentedbyalowerpercentageofthetotalspopulation.
oIwillthengetintoinheritancepatterns,andexplainthatwecanuseratiosof
individualswithdominanttraitsversusindividualswithrecessivetraitstodetermine
ifanalleleisdominantorrecessive.
Timetoanswerlabquestions[10min]:
Studentswillhavetherestofthetimeinclasstofinishthequestionsaskedinthelab.
Whatisnotfinishedinclassishomework.