Acid and Base Lab2
Acid and Base Lab2
Acid and Base Lab2
Introduction
The student will determine the pH of various substances and will also
determine how some of these substances can affect the environment.
Materials
pH paper and chart
well plate
pipettes
various household solutions
Procedure
1. Put a drop of each substance, one per well on the well plate. Remember, do
NOT come in contact with any of the materials and do not mix-n-match
pipettes.
2. Using your knowledge of the pH scale, hypothesize whether the liquid
would be an acid or base. Record this number on your data table.
3. Pick up a piece of pH paper with dry hands.
4. Touch the pH paper to the liquid in well A and remove it. Compare the
color of the paper with that on the pH chart. Record your observations on the
chart below.
5. Repeat the procedure with the rest of the slides.
Clean-up
1.
2.
3.
4.
Data
ID
A
B
C
D
E
F
G
H
I
J
K
L
M
Item
Hypothesis
2
4
8
3
1
5
5
7
8
9
Borax
Sugar water
Baking soda
Salt water
Vinegar
Dish soap
Ketchup
Alka-Seltzer
Water
Apple Juice
pH
9
6
8
6
1
6
2
5
7
3
Acid
Base
Base
Neutral
Acid
Base
Acid
Acid
Acid
Acid
Acid
Neutral
Acid
Analysis
1. Which of the liquids had the lowest pH? Vinegar
2. Which of the liquids had the highest pH? Borax .
3. Which of the liquid(s) were closest to being neutral? The liquid which was almost
neutral were Salt and sugar water, also dish soap. However only water was neutral.
4. If the pH of a sample was 3, how many times more acidic is it than a solution with a pH
of 6? It is two times more acidic than the substance with 6.
5. How might one correct the pH of a lake with a reading of 3? Explain your reasoning. By
becoming more ecofriendly, and trying to not throw trash in lakes and water.
Biological Applications of pH:
1. What is the pH of human blood? 7
2. What would happen to our bodies if blood pH was changed or altered?
If our blood was altered in any way, our bodies could either adapt to the change or we
would die from being unable to use the blood thanks to the higher acidic levels.
3. The pH of stomach acid is 2, why is this both good, but possibly destructive to our bodies?
The acidic level of the stomach is good, since it helps break down food which give us
energy, and breaks down waste as well.
4. What could happen to our local environment if a company pumped materials into the
atmosphere which created a drastic pH change in the rain water? The environment would be in
danger, since acidic levels in the rain would increase.
5. When we eat too many tomato products (ex. Salsa) our stomachs can become upset. Using
your understanding of pH and the pH chart in your text, why does an antacid make you feel
better? The acid levels increase because of the salsa, causing the stomach to become upset,
and an antacid regulates your stomachs acidic level.