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Absorbance V.S. Time Enzyme Lab

This document contains data from an enzyme kinetics experiment measuring absorbance over time for solutions with different concentrations of substrate. Absorbance readings were taken every 30 seconds over a period of 10 minutes for solutions with substrate concentrations of 608 μm, 304 μm, and 152 μm. Linear trendlines are fitted to the data for each substrate concentration, with higher concentrations showing a faster initial rate of absorbance increase.

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alex_wang_1
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33 views

Absorbance V.S. Time Enzyme Lab

This document contains data from an enzyme kinetics experiment measuring absorbance over time for solutions with different concentrations of substrate. Absorbance readings were taken every 30 seconds over a period of 10 minutes for solutions with substrate concentrations of 608 μm, 304 μm, and 152 μm. Linear trendlines are fitted to the data for each substrate concentration, with higher concentrations showing a faster initial rate of absorbance increase.

Uploaded by

alex_wang_1
Copyright
© © All Rights Reserved
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
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Seconds

0
10
20
30
60
90
120
150
180
210
240
270
300
330
360
390
420
450
480
510
540
570
600

608 m
0.061
0.088
0.1
0.145
0.274
0.418
0.566
0.72
0.875
1.04
1.2
1.36
1.5
1.66
1.76
1.9

304 m
0.022
0.036
0.057
0.077
0.14
0.211
0.288
0.369
0.45
0.53
0.618
0.702
0.79
0.88
0.975
Absorbance
1.07
(410 nm)
1.16
1.25
1.35
1.44
1.54
1.64
1.72

Ab
2

f(x) = 0.0049068929x

1.8
1.6

f(x) = 0.002866835

1.4
1.2
1

f(x) = 0.001826621

0.8
0.6

f(x) = 0.0008858689x

0.4
0.2
0
0

100

rbance
0 nm)

152 m
6.5 pH
0.03
0.028
0.04
0.038
0.05
0.069
0.006
0.08
0.009
0.11
0.136
0.132
0.18
0.152
0.229
0.174
f(x) = 0.0049068929x
+
0.0077484704
0.29
0.2
0.335
0.224
f(x) = 0.0028668358x
0.388
- 0.0361769879
0.446
0.504
0.562
0.62
f(x) = 0.0018266216x - 0.0290658499
0.68
0.744
0.81
0.86
f(x) = 0.0008858689x
+ 0.0436294037
0.915
0.97
1.02
1.07

Absorbance v.s. Time Enzyme Lab

2
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
0

100

200

300

400

Time (in sec)

500

600

ab

608 m
Linear
(608 m)
304 m

600

700

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