WBI03 Jan 2015 MS

Mark Scheme (Results)
January 2015
Pearson Edexcel International

Advanced Subsidiary Level
in Biology (WBI03) Paper 01
Edexcel and BTEC Qualifications

Edexcel and BTEC qualifications are awarded by Pearson, the UKs largest awarding body.
We provide a wide range of qualifications including academic, vocational, occupational and
specific programmes for employers. For further information visit our qualifications
websites at www.edexcel.com or www.btec.co.uk. Alternatively, you can get in touch with
us using the details on our contact us page at www.edexcel.com/contactus.
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through innovation in education. Find out more about how we can help you and your
students at: www.pearson.com/uk
January 2015
Publications Code IA040379*
All the material in this publication is copyright
Pearson Education Ltd 2015
General Marking Guidance
All candidates must receive the same treatment. Examiners must

mark the first candidate in exactly the same way as they mark the
last.
Mark schemes should be applied positively. Candidates must be

rewarded for what they have shown they can do rather than
penalised for omissions.
Examiners should mark according to the mark scheme not according

to their perception of where the grade boundaries may lie.
There is no ceiling on achievement. All marks on the mark scheme

should be used appropriately.
All the marks on the mark scheme are designed to be awarded.

Examiners should always award full marks if deserved, i.e. if the
answer matches the mark scheme.
Examiners should also be
prepared to award zero marks if the candidates response is not
worthy of credit according to the mark scheme.
Where some judgement is required, mark schemes will provide the

principles by which marks will be awarded and exemplification may
be limited.
When examiners are in doubt regarding the application of the mark

scheme to a candidates response, the team leader must be
consulted.
Crossed out work should be marked UNLESS the candidate has

replaced it with an alternative response.
Question
Number
1(a)(i)
Answer
Additional Guidance
Mark
1. (temperature) 50 - 60C ;
2. {temperature controlled / eq} water bath / eq ;
3. (pH) 9 ;
ACCEPT incubator/oven
IGNORE temperature
controlled room
4. reference to buffer ;
(4)
Question
Number
1(a)(ii)
Answer
Additional Guidance
Mark
1. hot water / scalding risk, wear a lab coat / (heat

resistant) gloves ;
2. (Bunsen) risk of burning, keep away from flame ;
3. alkaline solution risk to skin or eyes, wear goggles or
gloves / lab coat / wash affected area immediately ;
4. skin contact allergy, wear gloves / lab coat / wash
affected area immediately / use coated enzyme ;
5. inhalation allergy, wear mask / use coated enzyme ;
6. spillages, clean up immediately ;
7. ethanol is flammable, keep it away from naked flames
(3)
Question
Number
1(b)
Answer
1. correct readings from the graph ;
2. calculation of rate ;
3. appropriate units e.g. a.u. per second ;
Additional Guidance
Mark
IGNORE number of decimal

points
2. accept answer in the range
0.42 to 0.45
correct answer with units
gains 3 marks
(3)
Question
Number
1(c)(i)
Answer
A axes correct (x enzyme
concentration, y rate) ;
Additional Guidance
Mark
MAX 3 if either scale is non-linear or if graph uses less

than half of the grid
L axes correctly labelled: lipase

concentration % and initial
rate (of reaction) arbitrary
units per second ;
P correct plotting ;
S suitable line ;
S accept points joined by straight lines or smooth

curve
(4)
Question
Number
1(c)(ii)
Answer
Additional Guidance
Mark
1. more readings / repeat / need to know how many

times it was repeated / eq ;
2. (idea of replication) under same conditions ;
(2)
3. standard deviation / standard error / range / eq ;
Question
Number
1(c)(iii)
Answer
Additional Guidance
Mark
1. recommended enzyme concentration, 3% ;

2. idea that use of high concentrations of lipase is more
expensive ;
3. idea of using concentration that produces biofuel fast
enough / idea of little {increase / eq} above 3% ;
4. ref. to straight line part and levelling off ;
5. ref. to enzyme concentration limiting over straight line
part ;
6. ref. to substrate concentration being limiting when
levelled off ;
7. suitable manipulation of data to support any of the
points made ;
7. e.g. rate of reaction

increases by only 0.01 when
enzyme concentration
increases by 1.5
IGNORE manipulation if units
are present and incorrect
(4)
Question
Number
2(a)(i)
Answer
1. use of {bar / pie} chart ;
Additional Guidance
Mark
1. ACCEPT suitable stacked bar chart
2. comparison between the two trap

types ;
3. comparison between the three
species ;
4. clear labelling ;
(4)
Candidates do not need to include totals
Question
Number
2(a)(ii)
Answer
7/8/9;
Additional Guidance
Mark
ACCEPT 7 or 8 or 9
(1)
Question
Number
2(a)(iii)
Answer
1. idea that Steiner is better overall ;
Additional Guidance
Mark
1. ACCEPT converse
2. Steiner better at catching two of the three species /

{less / equally} effective for one of the species ;
3. idea of equal effectiveness for trapping Bactrocera
cacuminatus ;
4. suitable manipulation of data to support any of the
points made ;
e.g. 296 / 53.6% more flies
caught with Steiner
(4)
Question
Number
2(b)
Answer
Additional Guidance
Mark
1. release of sterile males ;

2. an explanation of how this reduces fruit fly
population ;
2. e.g. (no fertilisation)

therefore eggs cannot develop
(2)
Question
Number
2(c)(i)
Answer
ENVIRONMENTAL IMPLICATION:
1. using insecticides ;
Additional Guidance
Mark
Max 3 marks for either

environmental implication
or economic implication
2. killing beneficial species / build up along food chains

;
3. problems of reducing fruit fly population / eq ;
4. e.g failure of orchid pollination so orchids become
extinct ;
5. idea that this affects food chains ;
ECONOMIC IMPLICATION :
6. idea of increased costs or loss of income ;
7. Perspex expensive ;
8. labour costs ;
9. fewer orchids or more fruit to sell ;
(4)
Question
Number
2(c)(ii)
Answer
1. increased income / eq ;
Additional Guidance
Mark
mark points 1 and 2 or 3

and 4 are awarded in pairs
2. more money for {education / health / eq} ;

OR
3. more food produced / eq ;
4. {better nutrition / e.g} / better health / more
employment / eq ;
Question
Number
2(d)
Answer
1. date of {publication / paper} ;
(2)
Additional Guidance
Mark
IGNORE url of website
2. article title ;
3. title of journal ;
4. further detail about journal {e.g. volume number / part
number / pages }
(3)
Pearson Education Limited. Registered company number 872828

with its registered office at Edinburgh Gate, Harlow, Essex CM20 2JE

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Mark Scheme (Results)

Pearson Edexcel International

Edexcel and BTEC Qualifications

Pearson: helping people progress, everywhere

General Marking Guidance

All candidates must receive the same treatment. Examiners must

Mark schemes should be applied positively. Candidates must be

Examiners should mark according to the mark scheme not according

There is no ceiling on achievement. All marks on the mark scheme

All the marks on the mark scheme are designed to be awarded.

Where some judgement is required, mark schemes will provide the

When examiners are in doubt regarding the application of the mark

Crossed out work should be marked UNLESS the candidate has

1. hot water / scalding risk, wear a lab coat / (heat

IGNORE number of decimal

MAX 3 if either scale is non-linear or if graph uses less

L axes correctly labelled: lipase

S accept points joined by straight lines or smooth

1. more readings / repeat / need to know how many

1. recommended enzyme concentration, 3% ;

7. e.g. rate of reaction

1. ACCEPT suitable stacked bar chart

2. comparison between the two trap

2. Steiner better at catching two of the three species /

1. release of sterile males ;

2. e.g. (no fertilisation)

Max 3 marks for either

2. killing beneficial species / build up along food chains

mark points 1 and 2 or 3

2. more money for {education / health / eq} ;

IGNORE url of website

Pearson Education Limited. Registered company number 872828

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