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SBS Plate Microarray Auto Processor: Key Requirements

Microarrays Inc is developing an automated microarray plate processing device for Alabama A&M's senior design project. The device must be able to process up to 110 plates according to standard operating procedures, incorporating wash buffers at appropriate temperatures and intervals as specified. It must safely and effectively dry plates, be ergonomically friendly, allow pausing at any step, and be programmable to run alternate processes. The device will log temperature and humidity during processing and have LED status indicators.

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61 views2 pages

SBS Plate Microarray Auto Processor: Key Requirements

Microarrays Inc is developing an automated microarray plate processing device for Alabama A&M's senior design project. The device must be able to process up to 110 plates according to standard operating procedures, incorporating wash buffers at appropriate temperatures and intervals as specified. It must safely and effectively dry plates, be ergonomically friendly, allow pausing at any step, and be programmable to run alternate processes. The device will log temperature and humidity during processing and have LED status indicators.

Uploaded by

Cam Miller
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Microarrays Inc

601 Genome Way, Suite 3300, Huntsville, AL 35806


[email protected]
www.microarrays.com


Alabama A & M Senior Design Project September 2013

SBS Plate Microarray Auto Processor

Key Requirements
Power: 110V
Capacity: 110 plates
Process as defined by SOPs
o Incorporates 4 wash buffers at appropriate temperatures
o Alert user when going into block wash pause and require user
intervention to continue
- addition of ethanolamine will be required
- temperature of block solution needs to be 50C
o DI water rinses
o Intervals and timing as specified
o Drain to sink for disposal
Safely & effectively dries plates
Materials shall not interact with reagents
o Container materials
o Tubing
Ergonomically friendly
o Weight of handling 20lbs
o Sits waist high average user 5 2 female
o Benchtop device
Pause function be able to check on the process at any step
o Does not need to interrupt a fill sequence

Goals
Programmable to process using alternate times and/or iterations
o LabView is available at MI for generating an executable
o Program#1: Purge/Prime sequences if necessary
o Program#2: Full processing event
o Program#3: Partial processing event
Log temperature & humidity readings during the processing event(s)
LED indicator running vs stopped/paused
Ability to process 25 x 75 mm slides as well
Microarrays Inc
601 Genome Way, Suite 3300, Huntsville, AL 35806
[email protected]
www.microarrays.com
Rack to be safely loaded as a single piece
Minimal noise
Abort sequence
o Would safely terminate the procedure by draining all liquids and
drying plates/slides

Definitions
Plate glass substrate on which arrays are printed
o Dimensions: 110 x 74 x 1.0 mm ( 0.1 thickness)
o Weight: 18 g ( 0.5 g) plate type Schott
o Weight: 21 g ( 0.5 g) plate type Alternate Supplier

Wash buffers liquid reagents used in the processing of microarrays.
o Triton - non-ionic surfactant
o HCl Hydrochloric acid
o KCl Potassium chloride
o dH2O de-ionized water / lab water
o ethanolamine used as a blocking agent. Is sensitive to light and
oxygen; must be added immediately prior to use.

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