Scribd
Upload
19 views

Practical MICRO

The document outlines an internal practical examination for an enzyme and bioprocess technology course. It lists 7 experiments students can choose from to complete as part of the exam: 1) production and estimation of lipase or alkaline protease, 2) determination of thermal death time (TDT) or thermal death point (TDP) of microorganisms, 3) determination of Km and Vmax for invertase or other enzyme kinetics experiments, 4) immobilization of whole cells, 5) enzymatic determination of compounds, and 6) study of allosteric enzymes. The exam is worth a total of 50 marks and students must label glassware, write brief work plans, and complete a viva voce and journal.

Uploaded by

Adena Cleris
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
19 views

Practical MICRO

The document outlines an internal practical examination for an enzyme and bioprocess technology course. It lists 7 experiments students can choose from to complete as part of the exam: 1) production and estimation of lipase or alkaline protease, 2) determination of thermal death time (TDT) or thermal death point (TDP) of microorganisms, 3) determination of Km and Vmax for invertase or other enzyme kinetics experiments, 4) immobilization of whole cells, 5) enzymatic determination of compounds, and 6) study of allosteric enzymes. The exam is worth a total of 50 marks and students must label glassware, write brief work plans, and complete a viva voce and journal.

Uploaded by

Adena Cleris
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 1

BAHAGWAN MAHAVIR COLLEGE OF M. Sc.

BIOTECHNOLOGY, SURAT
INTERNAL PRACTICAL EXAMINATION 2011-12 SUB. : IBT 706: Enzyme and Bioprocess Technology
DATE: Instruction: For each exercise take separate answer book. Keep your flasks and test tubes labeled with your roll no. Clean all the glasswares before you leave the Laboratory. Ex.1 Carry out exercise ticked below. First write down the plan of work in brief. TOTAL : 50 Marks

[20 ]

[ [ [ [ [ [ [ [ [ [ [
EX. 3 Ex.4

] ] ] ] ] ] ] ] ] ] ]

(a) (b) (c) (d)

Production and estimation of Lipase. Production and estimation of Alkaline Protease. Determination of TDT of provided microorganism for design of sterilizer. Determination of TDP of provided microorganism for design of sterilizer. [15]

Ex.2 Write the plan of work for the marked experiment and proceed to perform the same. (a) (b) (c) (d) (e) (f) (g) Determination of Km and Vmax for invertase. Study the Time Course of reaction catalyzed by alkaline phosphatase. Estimation of KLa by sulphite oxidation method for two variables. Determine the thermostability of Horse radish peroxidase. Immobilization of whole cell by calcium alginate method. Enzymatic determination of 3-phosphoglyceric acid in sample.

Study the properties of an allosteric enzyme. (Yeast isocitrate dehydrogenase or alcohol dehydrasegenase ) Viva-voce Journal

[10] [05]

You might also like