Examination Papers 20082012

CONTENT
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CBSE Examination Paper2008 (Delhi) CBSE Examination Paper2008 (All India) CBSE Examination Paper2008 (Foreign) CBSE Examination Paper2009 (Delhi) CBSE Examination Paper2009 (All India) CBSE Examination Paper2009 (Foreign) CBSE Examination Paper2010 (Delhi) CBSE Examination Paper2010 (All India) CBSE Examination Paper2010 (Foreign) CBSE Examination Paper2011 (Delhi) CBSE Examination Paper2011 (All India) CBSE Examination Paper2011 (Foreign) CBSE Examination Paper2012 (Delhi) CBSE Examination Paper2012 (All India) CBSE Examination Paper2012 (Foreign) Important Diagrams

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EXAMINATION PAPERS 2008 CBSE (Delhi) SETI

Time allowed: 3 hours Maximum marks: 70 General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. Section A contains 8 questions of 1 mark each, Section B is of 10 questions of 2 marks each, Section C has 9 questions of 3 marks each whereas Section D is of 3 questions of 5 marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.
SECTIONA

1. 2. 3. 4. 5. 6.

What causes speciation according to Hugo de Vries? When and why do some animals like frogs hibernate? List any two economically important products for humans obtained from Apis indica. Name the Indian variety of rice patented by an American company. What role do macrophages play in providing immunity to humans? Name the parts A and B of the transcription unit given below.
Structural gene 3' A 3' B

7. Name the worlds most problematic aquatic weed. What is the nature of the water body in which the weeds grow abundantly? 8. What is the major difference you observe in the offsprings produced by asexual reproduction and in the progeny produced by sexual reproduction?
SECTIONB

9. The flower of brinjal is referred to as chasmogamous while that of beans is cleistogamous. How are they different from each other? 10. Name the interaction in each of the following: (a) Cuckoo lays her eggs in the crows nest. (b) Orchid grows on a mango tree. (c) Ticks live on the skin of dogs. (d) Sea anemone is often found on the shell of hermit crab. 11. A man with blood group A married a woman with B group. They have a son with AB blood group and a daughter with blood group O. Work out the cross and show the possibility of such inheritance.

Xam idea BiologyXII OR The male fruit fly and female fowl are heterogametic while the female fruit fly and the male fowl are homogametic. Why are they called so? 12. Why is using tobacco in any form injurious to the health? Explain. 13. Differentiate between a detritivore and a decomposer giving an example of each. 14. A mother of one year old daughter wanted to space her second child. Her doctor suggested CuT. Explain its contraceptive actions. 15.
a DNA b DNA T T C G G C T T A A A A

Study the linking of DNA fragments shown above: (i) Name a DNA and b DNA. (ii) Name the restriction enzyme that recognises this palindrome. (iii) Name the enzyme that can link these two DNA fragments. 16. What is divergent evolution? Explain taking an example of plants. 17. Name the blank spaces a, b, c and d in the table given below: Type of Microbe Fungus Bacterium c Yeast Name a Acetobacter aceti Aspergillus niger d Commercial Product Penicillin b Citric acid Ethanol

18. Thermal power plants are inevitable in an industrial and densely populated country like ours. What harm do they do to the environment? Also mention any precaution that could be taken to save our environment.
SECTIONC

19. Draw a labelled diagram of the microscopic structure of a human sperm. 20. Expand MOET. Explain the procedure of this technology in cattle improvement. 21. One of the codons on mRNA is AUG. Draw the structure of tRNA adapter molecule for this codon. Explain the uniqueness of this tRNA.

Examination Papers

22. Study the population growth curves in the graph given below and answer the questions that follow:
a Population density (N) K

Time (t)

(i) Identify the growth curves a and b. (ii) Which one of them is considered a more realistic one and why? dN K - N (iii) If = rN is the equation of the logistic growth curve, what does K stand for? dt K (iv) What is symbolised by N? 23. How and why is the bacterium Thermus aquaticus employed in recombinant DNA technology? Explain. OR (a) What are molecular scissors? Give one example. (b) Explain their role in recombinant DNA technology. 24. Given below is a part of the template strand of a structural gene: TAC CAT TAG GAT (a) Write its transcribed mRNA strand with its polarity. (b) Explain the mechanism involved in initiation of transcription of this strand. 25. (i) How and at what stage does Plasmodium enter into a human body? (ii) With the help of a flow-chart, only show the stages of asexual reproduction in the life cycle of the parasite in the infected human. (iii) Why does the victim show symptoms of high fever? 26. It has been recorded that the temperature of the earths atmosphere has increased by 0.6C. (a) What has caused this increase? (b) Explain its consequences. 27. Explain the pattern of inheritance of haemophilia in humans. Why is the possibility of a human female becoming a haemophilic extremely rare? Explain.
SECTIOND

28. Draw a labelled diagram of the sectional view of a mature pollen grain in angiosperms. Explain the functions of its different parts. OR Give a schematic representation of oogenesis in humans. Mention the number of chromosomes at each stage. Correlate the life phases of the individual with the stages of the process. 29. (a) Mention the role of vectors in recombinant DNA technology. Give any two examples. (b) With the help of diagrammatic representation only, show the steps of recombinant DNA technology.

Xam idea BiologyXII OR (a) What is plasmid? (b) What is meant by ADA deficiency? How is gene therapy a solution to this problem? Why is not a permanent cure? 30. Explain Hershey-Chase experiment. What was proved through this experiment? OR (a) A true breeding pea plant, homozygous for inflated green pods is crossed with another pea plant with constricted yellow pods (ffgg). What would be the phenotype and genotype of F1 and F2 generations? Give the phenotype ratio of F2 generation. (b) State the generalisation proposed by Mendel on the basis of the above mentioned cross.

Examination Papers

SETII
(Uncommon Questions to Set I) SECTIONA

2. Which one of the two, stenothermals or eurythermals, shows wide range of distribution on earth and why? 5. Recently chikungunya cases were reported from various parts of the country. Name the vector responsible. 6. Name the components a and b in the nucleotide with a purine, given below:
H a C H b

8. Name the technique used for separating DNA fragments in the laboratory.
SECTIONB

10. Name the interaction in each of the following: (a) Cuscuta growing on a shoe flower plant. (b) Mycorrhizae living on the roots of higher plants. (c) Clown fish living among the tentacles of sea anemone. (d) Koel laying its eggs in crows nest. 17. Name the blank spaces a, b, c and d given in the following table: Type of Microbe Bacterium Fungus c Fungus Name a b Monascus purpureus Penicillium notatum
SECTIONC

Commercial Product Lactic acid Cyclosporin A Statins d

21. The length of a DNA molecule in a typical mammalian cell is calculated to be approximately 2.2 metres. How is the packaging of this long molecule done to accommodate it within the nucleus of the cell? 24. Draw a labelled diagram of sectional view of a human ovary showing various stages of follicles growing in it. 27. Why should biological control of pests and pathogens be preferred to the conventional use of chemical pesticides? Explain how the following microbes act as biocontrol agents: (a) Bacillus thuringiensis (b) Nucleopolyhedrovirus

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SECTIOND

Xam idea BiologyXII

30. (a) Draw a diagrammatic sketch of the sectional view of a typical anatropous ovule. (b) List the components of the embryo sac and mention their fate on fertilisation. OR (a) Give a schematic representation of spermatogenesis in humans. (b) At which stage of life does gametogenesis begin in human male and female respectively? (c) Name the organs where gametogenesis gets completed in human male and female respectively.

Examination Papers

SETIII
(Uncommon Questions to Set I and II) SECTIONA

1. What causes swelling of the lower limbs in patients suffering from filariasis? 4. List any two adaptive features evolved in parasites enabling them to live successfully on their hosts. 6. Name the types of synthesis a and b occurring in the replication fork of DNA as shown below:
5'

si

he nt sy

sy

nt he

si s

SECTIONB

10. Name the interaction in each of the following: (a) Ascaris worms living in the intestine of human. (b) Sucker fish attached to the shark. (c) Smaller barnacles disappeared when Balanus dominated in the coast of Scotland. (d) Wasp pollinating fig inflorescence. 17. Name the blank spaces a, b, c and d given in the following table: Type of Microbe Bacterium b Fungus Bacterium Name a Aspergillus niger Trichoderma polysporum d
SECTIONC

Commercial Product Clot buster enzyme Citric acid c Butyric acid

20. Explain the process of charging of tRNA. Why is it essential in translation? 24. How do organisms manage with stressful conditions existing in their habitat for short duration? Explain with the help of one example each.

Xam idea BiologyXII 25. A non-haemophilic couple was informed by their doctor that there is possibility of a haemophilic child be born to them. Explain the basis on which the doctor conveyed this information. Give the genotypes and the phenotypes of all the possible children who could be born to them.
SECTIOND

28. A true breeding pea plant homozygous for axial violet flowers is crossed with another pea plant with terminal white flowers (aavv). (a) What would be the phenotype and genotype of F1 and F2 generations? (b) Give the phenotypic ratio of F2 generation. (c) List the Mendels generalisations that can be derived from the above cross. OR (a) Explain Griffiths series of experiments where he witnessed transformation in bacteria he worked with. (b) Name the scientists responsible for determining the biochemical nature of transforming principle in Griffiths experiments. What did they prove?

Examination Papers

SOLUTIONS
Delhi -2008 (Set I, II, III) SETI SECTIONA

1. Mutation. 2. When the animals are not able to tolerate the stressful conditions like low temperature, they hibernate to avoid the stress by escaping in time since they can not migrate. 3. Honey and Beeswax. 4. Basmati variety of rice. 5. Macrophages destroy the microbes (by phagocytosis) and provide protection against diseases. 6. A is promoter sequence of DNA. B is coding strand. 7. Water hyacinth (Eichhornia crassipes). They are eutrophic water bodies. 8. Offsprings produced through asexual reproduction are genetically identical (clone) to each other as well as to their parents, while sexually produced offsprings show genetic variations leading to evolution.
SECTIONB

9. Brinjal has chasmogamous flowers, as they are open with exposed stamen and stigma. Such flowers show cross-pollination as well as self-pollination. On the other hand, beans has cleistogamous flowers, as they never open at all, even at maturity, they also show selfpollination. 10. (a) Brood parasitism (b) Commensalism (c) Parasitism (d) Commensalism. 11. Since, son has blood group AB and daughter has blood group O then the possible genotype of man will be I A i and that of female will be I B i. The desired cross would be as follows:
Parents Genotypes Gametes F1 generation I
A A

Man I i i I I
A B B A

I i I i Blood group A i i Blood group O

A B

Woman I Bi i

I I Blood group AB I Bi Blood group B

Thus, the F1 progeny can have all the four possible blood groups, i.e., A, B, AB and O. OR The male fruit fly has XY sex chromosomes and produces two types of gametes hence it is called heterogametic while female fowl has ZW sex chromosomes thereby producing two types of gametes thus they are called heterogametic. Female fruit fly has two X chromosomes, i.e., XX and produces similar gametes hence they are called homogametic. Also, male fowl has two Z chromosomes and produces similar gametes thus they are called homogametic.

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Xam idea BiologyXII 12. Tobacco in any form is injurious to health because: (i) Smoking of tobacco is associated with cancers of lung, urinary bladder, throat and lungs, bronchitis, coronary heart disease, gastric ulcer, etc. (ii) Smoking increases carbon monoxide (CO) content in blood and reduces the concentration of haem-bound oxygen, thus causing oxygen deficiency in the body. (iii) Oral cancer is mostly due to chewing of tobacco. (iv) Nicotine, the main constituent of tobacco stimulates adrenal gland to release adrenaline and nor-adrenaline into blood which raises the blood pressure and increases heartbeat. 13. Detritivore Decomposers They are organisms which feed on detritus and They are organisms which by secreting break them into smaller particles, e.g., enzymes break down complex organic matter earthworm. into inorganic substances, e.g., some bacteria and fungi. 14. CuT increase phagocytosis of sperms within the uterus and the Cu2+ ions released suppress sperm motility and the fertilising capacity of sperms. 15. (i) a is vector/plasmid DNA. b is foreign DNA (ii) EcoRI (iii) DNA ligase. 16. It is the evolutionary process in which same structure develops along different directions due to adaptations to different needs. The thorns of Bougainvillea and the tendrils of Cucurbita are homologous, as they are modified stems. 17. (a) Penicillium notatum (b) Acetic acid (c) Fungus (d) Saccharomyces cereviseae. 18. The particulate and gaseous pollutants are released from thermal power plants which produces harmful effects. These pollutants can cause: (i) breathing or respiratory symptoms when inhaled deep into the lungs. (ii) irritation, inflammations and damage to lungs and premature death. Precautionary measures include the use of electrostatic precipitators by which 99% of the particulate matters can be removed from the exhaust.
SECTIONC

19. Structure of a sperm

Plasma membrane Acrosome Head Nucleus containing chromosomal material

Neck Middle piece Mitochondria (energy source for swimming)

Tai l

Examination Papers

11

20. MOET stands for Multiple Ovulation Embryo Transfer Technology. Procedure: (i) In this method a cow is administered hormones, with FSHlike activity, to induce follicular maturation and superovulation. (ii) Due to superovulation the cow produces more than 68 eggs per cycle instead of one egg per cycle. (iii) This superovulated cow is mated either with an elite bull or artificially inseminated. (iv) The fertilised eggs at 832 cells stages are recovered non-surgically and transferred to surrogate mothers. 21. This tRNA is specific for amino acid Methionine and it also acts as initiator codon (initiator tRNA)
fMet

tRNA

U A A U 5'

C Anticodon G Codon

mRNA 3'

Initiator tRNA

22. (i) a is exponential growth curve or J-shaped . b is logistic growth curve or S-shaped. (ii) Logistic growth curve (b) is considered more realistic one because unlimited resources are not available in an ecosystem or in a habitat. (iii) K stands for carrying capacity. (iv) N indicates population density. 23. DNA polymerase (Taq polymerase) in recombinant technology is obtained from a bacterium, Thermus aquaticus. (i) DNA polymerase (thermostable) remain active during the high temperature induced during denaturation of double stranded DNA. (ii) This enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template. (iii) The repeated amplification is achieved by this enzyme and the amplified fragment if desired can be used to ligate with a vector for further cloning. OR (a) The restriction endonucleases are called molecular scissors, as they cut the DNA segments at particular locations, e.g., EcoRI cuts DNA between bases G and A only when sequence GAATTC is present in the DNA. (b) The restriction enzymes cut the DNA strands a little away from the centre of the palindromic sites, but between the same two bases on the opposite strands. This leaves single stranded portions with overhanging stretches called sticky ends on each strand as

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Xam idea BiologyXII they form hydrogen bonds with their complementary cut counterparts. This stickiness at the ends facilitates the action of the enzyme DNA ligase. 24. (a) 5 AUG GUA AUC CUA3 (b) DNA-dependent RNA polymerase, catalyses transcription of mRNA. The strand of DNA with 3 5 polarity acts as the template. The RNA polymerase binds to the promoter site and starts the process of transcription. 25. (i) Plasmodium enters the human body as sporozoites (infectious form) through the bite of infected female Anopheles mosquito. (ii) Sporozoites are injected with the bite of Anopheles mosquito into the body. Sporozoites reach the liver through blood Parasite reproduces asexually in the liver cells, and by bursting the liver cells enter the blood. They enter the red blood cells Parasites reproduce asexually in red blood cells and by bursting them release haemozoin which causes cycles of fever Parasites enter new red blood cells Starts the sexual stages (gametocytes) in red blood cells. Stages of asexual reproduction in the life cycle of Plasmodium (iii) A toxic substance called haemozoin is released by the rupture of red blood cells which cause the chill and high fever recurring every three to four days. 26. (a) Increase in the level of greenhouse gases has led to considerable heating of earth leading to global warming. Earths surface re-emits heat in the form of infrared radiation but part of this does not escape into space as atmospheric gases, e.g., carbon dioxide, methane, etc. absorb a major fraction of it. The molecules of these gases radiate heat energy, a major part of which again comes to earths surface, thus heating it up. (b) The rise in temperature is leading to deleterious changes in environment and result in the odd climatic changes like EI Nino effect that leads to melting of polar ice-caps. This results in a rise in sea level and ultimately many coastal areas will be submerged. 27. Haemophilia is a sex-linked (X-chromosome) recessive disorder which shows its transmission from unaffected carrier female to some of the male progeny. A female carrier (heterozygous XX h female) transmits the haemophilic gene to 50% of her sons and a female sufferer (homozygous X h X h ) transmits the disease to all her sons and the daughters are carriers. The possibility of a female becoming haemophilic is extremely rare because she has to be homozygous (X h X h ) recessive for that trait, i.e., mother of such a female has to be at least a carrier and the father should be haemophilic.

Examination Papers
SECTIOND

13

28. Sectional view of a pollen

Exine Intine Vegetative cell

Generative cell

Functions: (i) The hard outer layer called exine is made up of sporopollenin which is a resistant organic material. Exine can withstand high temperature, strong acids and alkali thus provide protection. (ii) The intine has a prominent aperture called germ pore through which pollen tube comes out. (iii) Vegetative cell has abundant food reserve. (iv) Generative cell divides mitotically giving rise to two male gametes before pollen grains are shed (3-celled stage). OR Systematic representation of oogenesis

29. (a) Role of vectors: The vectors have the ability to replicate within the bacterial cells independent of the control of chromosomal DNA. If an alien piece of DNA is linked to the vector like bacteriophage or plasmid DNA, it can be made to multiply its number equal to the

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Xam idea BiologyXII copy number of the vector. Vectors are also used in the selection of recombinants from non-recombinants. Plasmids and bacteriophages are the most commonly used vectors. (b)
Foreign DNA Same restriction enzyme cutting both foreign DNA and vector DNA at specific point Vector DNA (plasmid)

Ligases join foreign DNA to plasmid

Transformation

E.coli Cells divide

Diagrammatic representation of steps in recombinant DNA technology

OR (a) Plasmid is a circular extra-chromosomal DNA molecule present in a bacterial cell, which replicate autonomously along with bacterial chromosomal DNA. (b) ADA deficiency: It refers to the deficiency of enzyme adenosine deaminase (ADA) caused by the deletion of the gene coding for this enzyme. This enzyme is crucial for the immune system to function. Gene therapy: Lymphocytes from the blood of the patient are grown in a culture outside the body. A functional ADA cDNA is then introduced using a retroviral vector into the lymphocytes, which in turn are then returned to the patient. However, as these cells are not immortal, the patient requires periodic infusion of such genetically engineered lymphocytes. 30. Procedure: (i) Hershey and Chase grew some bacteriophages on a medium that contained radioactive phosphorus (32P) and some in another medium with radioactive sulphur (35S). (ii) Bacteriophages grown in the medium containing radioactive phosphorus (32P) contained radioactive DNA.

Examination Papers

15

(iii) Similarly bacteriophages grown in the medium containing radioactive sulphur (35S) contained radioactive protein. (iv) Both the radioactive bacteriophage types were allowed to infect E. coli separately. (v) Soon after infection, the bacterial cells were gently agitated in blender to remove viral coats. (vi) The culture was also centrifuged to separate the bacteriophage particle from the bacterial cell. Observations and Conclusions: (i) Only radioactive 32P was found to be associated with the bacterial cell, whereas radioactive 35S was only found in surrounding medium and not in the bacterial cell. (ii) This indicates that only DNA and not protein coat entered the bacterial cell. (iii) This proves that DNA is the genetic material which is passed from virus to bacteria and not protein. OR (a)
Parents Genotypes Gametes F1 generation On selfing Gametes FG FfGg Fg fG fg FG Fg (Inflated, green pods) FFGG FG (Constricted, yellow pods)set ffgg fg FfGg (All Inflated green pods) FfGg fG fg

FG F2 generation: FG FFGG Inflated, Green FFGg Fg Inflated, Green FfGG fG Inflated, Green FfGg fg Inflated, Green

Fg FFGg Inflated, Green FFgg Inflated, Yellow FfGg Inflated, Green Ffgg Inflated, Yellow

fG FfGG Inflated, Green FfGg Inflated, Green ffGG Constricted, Green ffGg Constricted, Green

fg FfGg Inflated, Green Ffgg Inflated, Yellow ffGg Constricted, Green ffgg Constricted, Yellow

F2 phenotypic ratio: Inflated 9 : : Inflated Yellow Pods 3 : : Constricted Green Pods 3 : : Constricted Yellow Pods 1 Green Pods

(ii) Mendels Law of Independent Assortment: This law states the different factors or allelomorphic pair in gametes and zygotes assort themselves and segregate independent of one another.

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Xam idea BiologyXII

SETII
SECTIONA

2. Eurythermals show a wide range of distribution on earth, as they show tolerance for wide range of temperatures. 5. Aedes mosquito is responsible for chikungunya cases. 6. a is phosphate group. b is nitrogenous base (Purine), i.e., Adenine or Guanine 8. Gel electrophoresis.
SECTIONB

10. (a) Parasitism (b) Mutualism (c) Commensalism (d) Brood parasitism 17. (a) Lactobacillus (b) Trichoderma polysporum (c) Fungus (d) Penicillin
SECTIONC

21. Eukaryotic cells possess a set of positively charged, basic proteins called histones which are rich in basic amino acid residues lysine and arginine. Histones are organised to form a unit of eight molecules, called histone octamer. The negatively charged DNA is wrapped around the positively charged histone octamer to form a structure called nucleosome. A typical nucleosome contains 200 bp of DNA helix and nucleosomes form the repeating unit of a structure of the nucleus called chromatin. 24. Diagrammatic section view of ovary:
Primary follicle Blood vessels Tertiary follicle Showing antrum Graafian follicle

Ovum Corpus Iuteum

Examination Papers

17

27. The chemical pesticides cause pollution of water bodies as well as ground water, besides getting stored in the plants. These chemicals are toxic and thus extremely harmful to human beings and other animals. (a) Bacillus thuringiensis: B. thuringiensis toxin genes are introduced into plants to make them resistant to attack by insects and pests. They are available in sachets as dried spores, which are mixed with water and sprayed onto vulnerable plants. When they are eaten by the insect larvae, the toxin is released in the gut and becomes active and kills the larvae. Specific Bt toxin genes obtained from Bacillus thuringiensis are used in several crop plants which make them resistant to the insect pest. (b) Nucleopolyhedrovirus: These viruses are excellent candidates for species-specific, narrow spectrum insecticidal applications. This is especially desirable when beneficial insects are being conserved to aid in an overall integrated pest management (IPM) programme.
SECTIOND

30. (a) A diagrammatic view of a typical anatropous ovule:

Hilum Funicle Micropyle Micropylar pole Outer integument Inner integument Nucellus Embryo sac

Chalazal pole

(b) The embryo sac contains 3 antipodals, 3 synergids, 1 egg and 2 polar nuclei. After positive pollenpistil interaction, the pollen tube develops and enters the ovule through synergids guided by filiform apparatus. One of the male gamete fertilises the female gamete to form diploid zygote. The another male gamete fuses with the secondary nucleus (polar nuclei if they are already fused) to form a triploid primary endosperm nucleus (PEN) that develops into endosperm. The three antipodals at chalazal end and synergids at micropylar end start degenerating. OR

18 (a) Schematic representation of spermatogenesis in human:

At puberty Spermatogonia Mitosis differentiation CHROMOSOMES NUMBER PER CELL 46

Xam idea BiologyXII

Primary spermatocytes 1st meiotic division

Secondary spermatocytes 2nd meiotic division Spermatids Differentiation Spermatozoa

23

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(b) Gametogenesis, i.e.. spermatogenesis in males and oogenesis in females starts at puberty. (c) In human males the process is completed in the testes (seminiferous tubules) while in the female it is completed in the fallopian tube (oviduct).

SETIII
SECTIONA

1. Wuchereria (W. bancrofti and W. malayi)the filarial worms cause inflammation of the lymphatic vessels of the lower limbs in patients suffering from filariasis. 4. Parasites have evolved the following adaptive features: (I) Presence of adhesive organs or suckers to cling onto the host. (ii) Loss of their digestive system. (iii) Loss of unnecessary sense organs. (iv) High reproductive efficiency. 6. a is continuous synthesis. b is discontinuous synthesis. SECTIONB 10. (a) Parasitism (b) Commensalism (c) Competition (d) Mutualism

Examination Papers 17. (a) (b) (c) (d) Streptococcus Fungus Cyclosporin A Clostridium butylicum
SECTIONC

19

20. Charging of tRNA: The amino acids are activated in the presence of ATP and linked to their cognate tRNA. This process is called charging of tRNA or amino-acylation of tRNA. When two such charged tRNAs are brought close enough, the formation of peptide bond between the corresponding amino acids would be favoured energetically. The presence of a catalyst would enhance the rate of peptide bond formation. 24. If the stressful conditions remain for short duration the organisms have two alternatives to mange, i.e., either by (I) migration or (ii) suspend. (I) Migration: The temporary movement of organism from the stressful habitat to a more hospitable area and return when stressful period is over is called migration. For example, migratory birds from Siberia come to Keoladeo National Park (Bharatpur) every winter. (ii) Suspend: Those animals who fail to migrate, might avoid the stress by escaping in time. Hibernation of bears during winter or aestivation of snails and fish to avoid in summer are examples of this phenomenon. 25. On the basis of pedigree analysis, the doctor conveyed this information. Pedigree study provides a strong tool, which is utilised to trace the inheritance of a specific trait, abnormality or disease. Since, both the parents are non-haemophilic, their genotypes will be: Father XY (Normal) Mother XX h (Carrier, Non-haemophilic)
Parents Genotypes Gametes F1 generation X Y Genotype Phenotype XX Normal female 1 X Father XY Y X XX Normal female XY Normal male Xh XX h Carrier non-haemophilic female X hY Haemophilic male XY Normal male : 1 XhY Haemophilic male 1 X Mother XXh Xh

XXh Carrier non-haemophilic female 1

SECTIOND

28. Parents
Genotypes Gametes F1 generation

Axial, Violet flowers

AAVV AV

Terminal, White flowers

aavv av

AaVv (Axial, Violet flowers)

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On selfing Gametes AV AaVv Av aV av AV Av AaVv aV av

Xam idea BiologyXII

AV F2 generation AV AAVV Axial, Violet Av AAVv Axial, Violet aV AaVV Axial, Violet av AaVv Axial Violet

Av AAVv Axial, Violet AAvv Axial, White AaVv Axial, Violet Aavv Axial, White

aV AaVV Axial, Violet AaVv Axial, Violet aaVV

av AaVv Axial, Violet Aavv Axial, White aaVv

Terminal, Violet Terminal, Violet aaVv aavv

Terminal, Violet Terminal, white

(a) Phenotype of F1 generation: All axial, violet flowers Genotype of F1 generation: AaVv (b) Phenotypic ratio of F2 generation: Axial violet flowers 9 : Axial white flowers 3 : Terminal violet flowers 3 : Terminal white flowers 1

(c) Law of independent assortment: This law states that the different factors or allelomorphic pairs in gametes and zygotes assort themselves and segregate independently of one another. OR (a) Frederick Griffith (1928) conducted experiments with Streptococcus pneumoniae (bacterium causing pneumonia). He observed two strains of this bacteriumone forming smooth shiny colonies (S-type) with capsule, while other forming rough colonies (R-type) without capsule. When live S-type cells were injected into mice, they died due to pneumonia. When live R-type cells were injected into mice, they survived. When heat-killed S-type cells were injected into mice, they survived and there were no symptoms of pneumonia. When heat-killed S-type cells were mixed with live R-type cells and injected into mice, they died due to unexpected symptoms of pneumonia. He concluded that heat-killed S-type bacteria caused a transformation of the R-type bacteria into S-type bacteria but he was not able to understand the cause of this bacterial transformation. (b) Oswald Avery, Colin MacLeod and Maclyn McCarty repeated Griffiths experiment in an in vitro system in order to determine biochemical nature of transforming principle. They concluded that DNA is the hereditary material.

EXAMINATION PAPERS 2008 CBSE (All India) SETI

Time allowed: 3 hours Maximum marks: 70

General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. Section A contains 8 questions of 1 mark each, Section B is of 10 questions of 2 marks each, Section C has 9 questions of 3 marks each whereas Section D is of 3 questions of 5 marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.
SECTIONA

1. 2. 3. 4. 5. 6.

Name any two vertebrate body parts that are homologous to human forelimbs. When and why do some animals like snails go into aestivation? What is the economic value of Spirulina? What was the speciality of the milk produced by the transgenic cow Rosie? How do neutrophils act as a cellular barrier to pathogens in humans? Mention the polarity of the DNA strands ab and cd shown in the replicating fork given below.
b d

7. Mention any two significant roles predation plays in nature. 8. Why is the polar region not a suitable habitat for tiny humming birds?
SECTIONB

9. (a) Expand IUD. (b) Why is hormone releasing IUD considered a good contraceptive to space children?

22

Xam idea BiologyXII 10. Name the interaction in each of the following: (a) Cuscuta growing on a shoe flower plant. (b) Mycorrhizae living on the roots of higher plants. (c) Clown fish living among the tentacles of sea anemone. (d) Koel laying her eggs in crows nest. 11. A plant of Antirrhinum majus with red flowers was crossed with another plant of the same species with white flowers. The plants of the F1 generation bore pink flowers. Explain the pattern of inheritance with the help of a cross. OR A woman with blood group O married a man with AB group. Show the possible blood groups of the progeny. List the alleles involved in this inheritance. 12. Why do sports persons often fall victim to cocaine addiction? 13. State the difference between the first trophic levels of detritus food chain and grazing food chain. 14. Coconut palm is monoecious while date palm is dioecious. Why are they called so? 15. How can DNA segments, separated by gel electrophoresis, be visualised and isolated? 16. How do Darwins finches illustrate adaptive radiation? 17. Name the blank spaces a, b, c and d from the table given below: Type of Microbe Bacterium Fungus c Fungus Name a b Monascus purpureus Penicillium notatum Commercial Product Lactic acid Cyclosporin A Statin d

18. DDT content in the water of a lake that supplies drinking water to the nearby villages, is found to be 0.003 ppm. The kingfishers of that area were reported to have 2 ppm of DDT. Why has the concentration increased in these birds? What harm will this cause to the bird population? Name the phenomenon.
SECTIONC

19. (a)
Ovarian hormone levels 1

Estrogen

Progesterone

10

15 20 Days

25

29

Read the graph given above and correlate the uterine events that take place according to the hormonal levels on: (i) 615 days

Examination Papers

23

(ii) 1625 days (iii) 2628 days (if the ovum is not fertilised) (b) Specify the sources of the hormones mentioned in the graph. 20. Explain the role of baculoviruses as biological control agents. Mention their importance in organic farming. 21. (a) Draw the structure of the initiator tRNA adaptor molecule. (b) Why is tRNA called an adaptor molecule? 22.
Population density (N) K

Time (t)

Study the population growth curves shown above: (i) Identify curves a and b. (ii) Mention the conditions responsible for the curves a and b respectively. (iii) Give the necessary equation for the curve b. 23. Why is Agrobacterium tumefaciens a good cloning vector? Explain. OR Explain the importance of (a) ori, (b) amp R and (c) rop in the E. coli vector shown below:
Eco R I Cla I Hind III

BamH I Pvu I ampR Pst I pBR322 tetR Sal I

ori

rop

Pvu II

24 24.
a U A CG A G A G A U U U b

Xam idea BiologyXII

Study the mRNA segment given above which is complete to be translated into a polypeptide chain. (i) Write the codons a and b. (ii) What do they code for? (iii) How is peptide bond formed between two amino acids in the ribosome? 25. (a) Name the infective stage of Plasmodium which Anopheles mosquito takes in along with the blood meal from an infected human. (b) Why does the infection cause fever in humans? (c) Give a flow chart of the part of the life-cycle of this parasite passed in the insect. 26. A factory drains its waste water into the nearby lake. It has caused algal bloom. (a) How was the algal bloom caused? (b) What would be the consequences? (c) Name the phenomenon that caused it. 27. Study the given pedigree chart and answer the questions that follow: I II

III

(a) Is the trait recessive or dominant? (b) Is the trait sex-linked or autosomal? (c) Give the genotypes of the parents in generation I and of their third and fourth child in generation II.
SECTIOND

28. (a) Draw a schematic labelled diagram of a fertilised embryo sac of an Angiosperm. (b) Describe the stages in embryo development in a dicot plant. OR (a) Draw a labelled diagram of a sectional view of human seminiferous tubule. (b) Differentiate between gametogenesis in human males and females on the basis of: (i) time of initiation of the process. (ii) products formed at the end of the process.

Examination Papers

25

29. Explain the steps involved in the production of genetically engineered insulin. OR (a) Name the nematode that infests and damages tobacco roots. (b) How are transgenic tobacco plants produced to solve this problem? 30. What is semi-conservative DNA replication? How was it experimentally proved and by whom? OR A homozygous tall pea plant with green seeds is crossed with a dwarf pea plant with yellow seeds: (i) What would be the phenotype and genotype of F1? (ii) Work out the phenotypic ratio of F2 generation with the help of a Punnett Square.

26

Xam idea BiologyXII

SETII
(Uncommon Questions to SetI) SECTIONA

2. Between amphibians and birds, which will be able to cope with global warming? Give reasons. 3. Name the two types of cells in which the HIV multiplies after gaining entry into the human body. 7. A multinational company outside India tried to sell new varieties of turmeric without proper rights. What is such an act referred to?
SECTIONB

10. Certain species of wasps are seen to frequently visit flowering fig trees. What type of interaction is seen between them and why? 18. Name the blank spaces a, b, c and d from the table given below: Type of Microbe Bacterium b Fungus Bacteria Name a Aspergillus niger Trichoderma polysporum d
SECTIONC

Commercial Product Clot buster enzyme Citric acid c Butyric acid

20. Name the pathogen that causes amoebiasis in humans. Give the symptoms and the mode of transmission of the disease. 22. How does Darwins theory of Natural Selection explain the appearance of new forms of life on earth? 23. How is the bacterium Thermus aquaticus employed in recombinant DNA technology? OR DNA being hydrophilic cannot pass through the cell membrane of a host cell. Explain how does recombinant DNA get introduced into the host cell to transform the latter.
SECTIOND

29. A tall pea plant with yellow seeds (heterozygous for both the traits) is crossed with a dwarf pea plant with green seeds. Using a Punnett square work out the cross to show the phenotypes and the genotypes of F1 generation. OR (a) Why is DNA molecule a more stable genetic material than RNA? Explain. (b) Unambiguous, degenerate and universal are some of the salient features of genetic code. Explain.

Examination Papers

27

SETIII
(Uncommon Questions to SetI and II) SECTIONA

3. Suggest any two techniques which can help in early detection of bacterial viral infections much before the symptoms appear in the body. 6. Mention the carbon positions to which the nitrogenous base and the phosphate molecule are respectively linked in the nucleotide given below:
Phosphate H P C H Base B S

7. Given below are some human organs. Identify one primary and one secondary lymphoid organs: Liver, Thymus, Stomach, Thyroid, Tonsils.
SECTIONB

10. The clown fish lives among the tentacles of sea anemone. What is this interaction between them called and why? 17. Name the blank spaces a, b, c and d from the table given below: Type of Microbe (i) Fungus (ii) c Scientific Name a Monascus purpureus
SECTIONC

Product Cyclosporin Statin

Medical Application b d

21. Name the type of immunity that is present at the time of birth in humans. Explain any two ways by which it is accomplished. 22. Study the graph given below and answer the questions that follow:

Dissolved oxygen Concentration

BOD Direction of flow of river

Sewage discharge

28

Xam idea BiologyXII (i) What is the relationship between dissolved oxygen and biochemical oxygen demand (BOD)? (ii) Mention their effect on aquatic life in the river. 26. Study the given pedigree chart and answer the questions that follow: I II

III

(a) Is the trait recessive or dominant? (b) Is the trait sex-linked or autosomal? (c) Give the genotypes of the parents shown in generation I and their third child shown in generation II and the first grandchild shown in generation III.
SECTIOND

28. Draw a labelled schematic structure of a transcription unit. Explain the function of each component in the unit in the process of transcription. OR A snapdragon plant homozygous for red flower when crossed with a white flowered plant of the same species produced pink flowers in F1 generation. (a) What is this phenotypic expression called? (b) Work out the cross to show the F2 generation when F1 was self-pollinated. Give the phenotypic and genotypic ratios of F2 generation. (c) How do you compare the F2 phenotypic and genotypic ratios with those of Mendelian monohybrid F2 ratios?

Examination Papers

29

SOLUTIONS
All India-2008 SETI SECTIONA

1. 2. 3. 4. 5. 6. 7.

8.

Wings of birds and forelimbs of horse. Snails undergo aestivation if they are unable to migrate in order to avoid stressful condition. Spirulina can serve as food rich in proteins, minerals, vitamins, fats and carbohydrates. Rosie produced milk with human alpha-lactalbumin and was nutritionally a more balanced product for human babies than natural cow-milk. Neutrophils in blood can phagocytose and destroy the microbes. a b has polarity 3 5 c d has polarity 5 3 Predation plays following two important roles in nature: (i) They keep the prey populations under control. (ii) They act as conduits for energy transfer across trophic levels. Small animals have larger surface area relative to their volume. They tend to lose body heat very fast when it is cold outside. They have to expend much energy to generate body heat through metabolism. This is the reason, why polar regions are not a suitable habitat for tiny humming birds.
SECTIONB

9. (a) IUDIntra Uterine Devices. (b) IUDs are considered as good contraceptives because: (i) they make the uterus unsuitable for implantation. (ii) it increases the phagocytosis of sperms within uterus and the Cu ions released suppress sperm motility and the fertilising capacity of sperms. 10. (a) Parasitism (b) Mutualism (c) Commensalism (d) Brood parasitism. 11. Inheritance of flower colour in Antirrhinum majus.
Parents Genotypes Red flowers RR Gametes R White flowers rr r

F1 generation On selfing Gametes F2 generation R Rr r

Rr (Pink flowers) R R RR Red flower Rr Pink flower Rr r r Rr Pink flower rr White flower

R r

30

Xam idea BiologyXII The above pattern shows law of independent assortment which states that when two pairs of traits are combined in a hybrid, segregation of one pair of characters is independent of the other pair of characters. OR
Parents Father (AB blood group) IA IB IA IB IA i (A-blood group) A B I Bi (Bblood group) Mother (O blood group) ii i

Genotype Gametes

F1 generation

The three alleles I , I , i are involved in this inheritance. 12. Cocaine has a potent stimulating action on central nervous system, producing a sense of euphoria and increased energy. Certain sports persons misuse it to enhance their performance. 13. First trophic level of First trophic level of S. No. detritus food chain grazing food chain (i) (ii) Decomposers are at the first trophic level. Producers are at the first trophic level.

They break down the complex organic They prepare complex organic molecules matter into simpler form by secreting from simple inorganic material with the enzymes. help of sun light.

14. Coconut palm is monoecious, as both the male and female reproductive structures are borne on the same plant (bisexual) while date palm is dioecious because male and female reproductive structures are borne on different plants (unisexual). 15. The separated DNA molecules are visualised only after staining DNA with ethidium bromide followed by exposure to UV radiation, as bright orange coloured bands. The separated bands of DNA (on the gel) are cut from the agarose gel and extracted from the gel piece and the process is called elution. 16. Darwin during his journey to Galapagos Islands observed that there were many varieties of small black birds later called Darwins finches. All the varieties he conjectured, evolved on the island itself. From the original seed-eating features, many other forms with altered beaks arose, enabling them to become insectivorous and vegetarian finches. This process of evolution of different species in a given geographical area starting from a point and literally radiating to other areas of geography (habitats) is called adaptive radiation. 17. (a) Lactobacillus (b) Trichoderma polysporum (c) Fungus (d) Penicillin. 18. DDT being a toxic substance gets accumulated in the organism and passes on to the next higher trophic level because it cannot be metabolised or excreted. Thus, concentration of DDT has increased in the birds in the given case. The high concentration of DDT disturbs the

Examination Papers

31

calcium metabolism in birds, causes thinning of eggshells, their premature breaking and eventually causes a decline in the bird population. The phenomenon is called biomagnification.
SECTIONC

19. (a) (i) It is the follicular phase where follicular stimulating hormones (FSH) stimulate the ovarian follicles to secrete estrogen which in turn stimulate the proliferation of the endometrium of the uterine wall. The endometrial lining begins to thicken. (ii) Secretion of progesterone maintains the endometrium which is necessary for implantation of fertilised ovum and other events of pregnancy. (iii) When fertilisation does not take place, the endometrium disintegrates, leading to menstruation marking a new cycle. (b) Estrogen is secreted by the ovarian follicles and progesterone is secreted by the corpus luteum. 20. Baculoviruses are pathogens that attack insects and other arthropods. Baculoviruses of genus Nucleopolyhedrovirus is used as biological control agents. They are excellent candidates for species-specific, narrow-spectrum insecticidal applications. They do not show negative impact on plants, birds, non-target insects. Importance in organic farming: Desirable when beneficial insects are being conserved to aid in an overall integrated pest management (IPM) programme. Its role in organic farming is that it conserves beneficial insects and kills harmful ones. 21. (a)
fMet

tRNA

U A A U 5'

C Anticodon G Codon Initiator tRNA

mRNA 3'

(b) tRNA is called an adaptor molecule because on one end it reads the code on mRNA and on other end it binds to specific amino acid. 22. (i) a is exponential growth curve. b is logistic growth curve. (ii) a when resources in the habitat is unlimited. b when resources in a habitat is limited. K - N (iii) dN / dt = rN K

32

Xam idea BiologyXII 23. Agrobacterium tumifaciens is a soil bacterium which causes disease in many dicot plants. It is able to deliver a piece of DNA known as T-DNA, to transform the normal cells into tumour cells and direct these tumour cells to produce the chemicals required by the pathogen. The tumour inducing (Ti) plasmid of Agrobacterium tumifaciens has been modified into a cloning vector which is no more pathogenic to the plants but still delivers genes of interest into a variety of plants. OR (a) ori: It is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. It is also responsible for controlling the copy number of the linked DNA. (b) amp R : The ligation of alien DNA is carried out at a restriction site present in any antibiotic resistance gene. (c) rop: It codes for the proteins involved in the replication of the plasmid. 24. (i) a is AUG b is UAA/UAG/UGA (ii) AUG codes for methionine (initiation codon). UAA/UAG/UGA does not code for any amino acid, i.e., it is a stop or terminating codon. (iii) There are two sites (P-site and A-site) in the large subunit of ribosome, where subsequent amino acids bind to and thus are close enough to form peptide bond. The ribosome also acts as a catalyst for the formation of peptide bond. 25. (a) Gametocytes (b) The parasites initially multiply within the liver cells and then attack red blood cells resulting in their rupture. These cells burst leading to the chill/shivering and fever due to release of toxic chemical substance haemozoin. (c) Female Anopheles mosquito takes up gametocytes. Fertilisation and development takes place in the mosquitos intestine. Mature infective sporozoites escape from the intestine and migrate to the salivary glands of mosquito. 26. (a) Algal bloom is caused due to accumulation of large amounts of nutrients present in the waste water. (b) It causes: (i) deterioration of water quality. (ii) increase in fish mortality rate. (iii) some bloom-forming algae are toxic to human beings and animals. (c) Accelerated eutrophication. 27. (a) Recessive trait. (b) Autosomal trait. (c) Genotypes of the parents in generation I is 'Aa' and 'Aa'. Genotypes in generation II of third child is 'aa' and fourth child is 'Aa'.

Examination Papers
SECTIOND

33

28. (a) Fertilised embryo sac showing zygote and Primary Endosperm Nucleus (PEN)
Degenerating synergids Zygote (2n) Primary endosperm cell (PEC) Primary endosperm nucleus (3n) (PEN)

Degenerating antipodal cells

(b)

Suspensor Radicle Zygote Cotyledon Heart-Shaped Embryo Globular Embryo Development of dicot embryo Plumule Mature

Stages in dicot embryo development: n The zygote undergoes transverse division forming a large basal cell and a small apical or terminal cell. n The large basal cell enlarges and undergoes transverse division to from a group of 610 cells called suspensor.

34
n

Xam idea BiologyXII The first cell of the suspensor towards the micropylar end is called haustorium, whereas the last cell of the suspensor toward the chalazal end is called hypophysis that later develops into radicle. n The smaller terminal or apical cell undergoes one vertical division. The two cells formed from terminal cell divide by a transverse division thus forming four embryonal cell (quadrant stage). n Finally these four cells divide vertically forming 8-celled proembryo (octate stage). Four cells at the apex give rise to plumule and another four give rise to hypocotyl except its tip. OR (a) Diagrammatic sectional view of a seminiferous tubule (enlarged)

Spermatozoa Spermatid Secondary spermatocyte Primary spermatocyte Sertoli cell

Spermatogonium

(b) S. No. (i) (ii) Process of gametogenesis Time of initiation of the process Males At puberty Females At puberty One ovum and two polar bodies are produced from one primary oocyte.

Products formed at the Four spermatozoa are end of the process produced from one primary spermatocyte.

29. Genetically engineered insulin: Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly, in 1983. It prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E.coli for production. The A and B chains produced were separated, extracted and combined by creating disulfide bonds to form human insulin. OR (a) Nematode Meloidegyne incognitia infects the roots of tobacco plant.

Examination Papers

35

(b) Transgenic tobacco plants are produced in following way: (i) To prevent the roots of tobacco plants from infections a strategy was adopted based on the process of RNA interference (RNAi). (ii) Nematode-specific genes were introduced into the host plants using Agrobacterium vectors. (iii) The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. (iv) These two RNAs being complementary to each other formed a double stranded RNA (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. (v) The parasite could not survive in a transgenic host expressing specific interfering RNA, thus plant got itself protected from the parasite. 30. Watson and Crick in 1953 proposed a scheme that DNA replication was semi-conservative. According to the scheme, the two parental strands separate and each strand acts as a template for synthesising a complementary strand over it. After completion of replication, each DNA had one parental strand and one newly synthesised strand. Experimental Proof for semi-conservative mode of DNA replication: n Matthew Meselson and Franklin Stahl in 1958 performed experiments on E. coli to prove that DNA replication is semi-conservative. n They grew E. coli in a medium containing 15NH4Cl (in which 15N is the heavy isotope of nitrogen) for many generations. n As a result, 15N got incorporated into newly synthesised DNA. n This heavy DNA can be differentiated from normal DNA by centrifugation in caesium chloride (CsCl) density gradient. n Then they transferred the cells into a medium with normal 14NH4Cl and took the samples at various definite time intervals as the cells multiplied. n The extracted DNAs were centrifuged and measured to get their densities. n The DNA extracted from the culture after one generation of transfer from the 15N medium to 14N medium (i.e., after 20 minutes) showed an intermediate hybrid density. n The DNA extracted from culture after two generations (i.e., after 40 minutes) showed equal amount of light DNA and hybrid DNA. OR
Parents Genotypes Gametes Tall Green seeds TTyy Ty Dwarf Yellow seeds ttYY tY

F1 Generation On selfing TtYy

TtYy, (Tall and yellow seeds) TtYy

Gametes

TY

Ty

tY

ty

TY

Ty

tY

ty

36
F2 generation

Xam idea BiologyXII

TY TY Ty tY ty
TTYY Tall, Yellow seeds TTYy Tall, Yellow seeds TtYY Tall, Yellow seeds TtYy Tall, Yellow seeds

Ty
TTYy Tall, Yellow seeds TTyy Tall, Green seeds TtYy Tall, Yellow seeds Ttyy Tall, Green seeds

tY
TtYY Tall, Yellow seeds TtYy Tall, Yellow seeds ttYY

ty
TtYy Tall, Yellow seeds Ttyy Tall, Green seeds ttYy

Dwarf, Yellow seeds Dwarf, Yellow seeds ttYy ttyy

Dwarf, Yellow seeds Dwarf, Green seeds

(i) Phenotype of F1Tall plants with yellow seeds. Genotype of F1TtYy. (ii) Phenotypic ratio of F2 generation: Tall plants, yellow seeds 9 : : Tall plants, green seeds 3 : : Dwarf plants, yellow seeds 3 : : Dwarf plants, green seeds 1

Examination Papers

37

SETII
SECTIONA

2. Birds being eurythermals can tolerate a wide range of temperature and thus will be able to cope with global warming more efficiently. 3. Macrophages and Helper T-lymphocytes. 7. Biopiracy.
SECTIONB

10. Mutualism is seen between them because both are equally benefitted. Female wasp lays eggs in fruits and uses developing seeds within the fruit for nourishing its larvae. In return, the wasp pollinates the fig inflorescence while searching for suitable egglaying site. 18. (a) Streptococcus (b) Fungus (c) Cyclosporin A (d) Clostridium butylicum.
SECTIONC

20. Entamoeba histolytica. Symptoms: (i) Constipation (ii) Abdominal pain and cramps (iii) Stool with excessive mucus and blood clots. Mode of transmission: (i) Houseflies act as mechanical carriers as they carry the parasite from the faeces of the infected person and food products. (ii) Drinking water and food contaminated by the faecal matter are the main source of infection. 22. Darwins theory of natural selection: n Varying degrees of similarities can be observed between existing life forms and those existing millions of years ago. n There has been gradual evolution of life forms with new forms arising at different periods of history. n Any population has built-in variations in characteristics which adapt it better to the environment. n The characteristics which enable some populations or individuals to survive better in natural conditions (climate, food, physical factors) would out-breed others (Survival of the fittest). n Those populations which are better fit (reproductively fit) in an environment will be selected by nature and will survive more (Natural selection). n Adaptability is inherited and fitness is the end result of ability to adapt and get selected by nature. 23. DNA polymerase (Taq polymerase) is obtained from a bacterium, Thermus aquaticus. (i) DNA polymerase (thermostable) remains active during the high temperature induced during denaturation of double-stranded DNA. (ii) This enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template. (iii) The repeated amplification is achieved by this enzyme and amplified fragment if desired can be used to ligate with a vector for further cloning.

38

Xam idea BiologyXII OR rDNA is introduced into the host cell by the following methods: (i) Heat shock method: In this method, DNA is treated with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. The rDNA is forced into the cell by incubating the cell with rDNA on ice, followed by placing them briefly at 42C (heat shock) and then putting them back on ice. (ii) Micro-injection: In this method, the rDNA is directly injected into the nucleus of an animal cell. (iii) Gene gun/Biolistics: In this method, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA.
SECTIOND

29.
Parents Genotypes Gametes TY Ty Tall plants with Yellow seeds TtYy tY TY F1 generation ty TtYy ty Ty TtYy tY ttYy Dwarf plants with Green seeds ttyy ty ty ttyy

The phenotype and genotype of F1 generation are Tall, yellow seeds : Tall, green seeds : Dwarf, yellow seeds : Dwarf, green seeds TtYy : Ttyy : ttYy : ttyy 1 : 1 : 1 : 1 OR (a) In DNA, presence of thymine at the place of uracil confers more stability to DNA. In the nucleotide of RNA the 2-OH group is a reactive group and this makes RNA labile and degradable, while DNA is chemically less reactive and structurally more stable. (b) Unambiguous: One codon specifies only one particular amino acid and not any other hence it is called unambiguous. Degenerate: Some amino acids specifies more than one codon hence the codon is said to be degenerate. Universal: A particular codon coding for an amino acid is same for all organisms (except in mitochondria and some protozoa).

Examination Papers

39

SETIII
SECTIONA

3. Enzyme Linked Immuno Sorbent Assay (ELISA), Polymerase Chain Reaction (PCR). 6. Nitrogenous base is linked to first carbon. Phosphate is linked to fifth carbon. 7. Primary lymphoid organ is thymus. Secondary lymphoid organ is tonsils.
SECTIONB

10. The interaction between clown fish and sea anemone is called commensalism because one organism is benefitted and the other is neither benefitted nor harmed. The clown fish gets protection from predators which stay away from the stinging tentacles of sea anemone. The sea anemone does not appear to derive any benefit by hosting the clown fish. 17. a is Trichoderma polysporum. b is immuno-suppressive agent. c is fungus. d is blood cholesterol lowering agent.
SECTIONC

21. Innate immunity is present at the time of birth in humans. It is accomplished by providing different types of barriers. (a) Physical barriers: These barriers do not allow pathogens and foreign agents to enter the body, e.g., skin, mucous membranes of digestive, respiratory and urinogenital tracts trapping microorganisms. (b) Physiological barriers: Sweat, tears, acid in the stomach and saliva prevent microbial growth. (c) Cellular barriers: WBCs (polymorphonuclear leukocytes and monocytes, natural killer lymphocytes) and macrophages phagocytose and destroy microbes. (d) Cytokine barriers: Interferons produced by virus-infected cells protect non-infected cells from further viral infection. 22. (i) BOD refers to the amount of oxygen consumed if all the organic matter in one liter of water were oxidised by bacteria. Thus, greater the BOD, lesser will be the dissolved oxygen in sewage discharge. (ii) Effects on aquatic life: (a) High BOD causes high mortality rate among aquatic animals. (b) The excessive nutrients facilitate algal growth causing algal bloom. 26. (a) Dominant. (b) Autosomal. (c) Genotype of parents in generation 1 is femaleaa and maleAa. Genotype of third child in generation II is Aa. Genotype of first grandchild in generation III is Aa.

40
SECTIOND

Xam idea BiologyXII

28. Schematic structure of a transcription unit:

Transcription start site Promoter 3' 5' Terminator 5' 3'

Structural gene

Template strand

Coding strand

(i) Promoter: It is the binding site for RNA polymerase for initiation of transcription. (ii) Structural gene: It codes for enzyme or protein for structural functions. (iii) Terminator: It is the region where transcription ends. OR (a) This phenotype expression is called incomplete dominance. (b)
Parents Genotypes Gametes F1-Generation On selfing Gametes R Rr r R F2 generation R RR Red flower Rr Pink flower : : 2 Pink flowers Rr Pink flower rr White flower : : White flowers 1 R Red flower RR R Rr (All pink flowers) Rr r r White flower rr r

r Phenotype Genotype Red flowers 1

(c) The phenotypic ratio deviates from a typical Mendelian monohybrid ratio of 3 : 1 to 1 : 2 : 1. Since the heterozygous hybrid individual shows a different phenotype, i.e., pink colour flowers. The genotypic ratio remains the same as the Mendelian monohybrid ratio, which is also 1 : 2 : 1.

EXAMINATION PAPERS 2008 CBSE (Foreign) SETI

Time allowed: 3 hours Maximum marks: 70 General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. Section A contains 8 questions of 1 mark each, Section B is of 10 questions of 2 marks each, Section C has 9 questions of 3 marks each whereas Section D is of 3 questions of 5 marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.
SECTIONA

1. Pick out the ancestral line of angiosperms from the list given below: Conifers, seed ferns, cycads, ferns. 2. When and why do some animals go into hibernation? 3. What is the economic value of Saccharum officinarum? 4. What does the organisation GEAC check with reference to genetic engineering? 5. In what way are monocytes a cellular barrier in immunity? 6. What are a and b in the nucleotide with purine represented below? H a C H S

b 7. About 200 species of Cichlid fish became extinct when a particular fish was introduced in Lake Victoria of Africa. Name the invasive fish. 8. What is the significance of the process of RNA interference (RNAi) in eukaryotic organisms?
SECTION-B

9. Name the blank spaces a, b, c and d from the table given below: Item (i) Pericarp (ii) b (iii) Embryonal axis (iv) d What it Represents in the Plant a cotyledon in seeds of grass family c remains of nucellus in a seed

42

Xam idea BiologyXII 10. In a particular plant species, majority of the plants bear purple flowers. Very few plants bear white flowers. No intermediate colours are observed. If you are given a plant with purple flowers, how would you confirm that it is a pure breed for that trait? Explain. OR A woman with B blood group married a man with A blood group. They had 2 sons and both had O group. Show the possibility of such an inheritance. List the alleles involved in determining the blood group. 11. Name the interaction in each of the following: (a) Clown fish living among the tentacles of sea anemone. (b) Sucker fish lives attached to the shark. (c) Smaller barnacles disappeared when Balanus dominated in the coast of Scotland. (d) Wasp pollinating fig inflorescence. 12. Due to undue peer pressure a group of adolescents started using opioids intravenously. What are the serious problems they might face in future? 13. Construct a grazing food chain and detritus food chain using the following, with 5 links each: Earthworm, bird, snake, vulture, grass, grasshopper, frog, decaying plant matter. 14. How do surgical procedures prevent conception in humans? Mention the way it is achieved in human males. 15. The following illustrates the linking of DNA fragments:
a DNA b DNA C G

(i) Name a and b . (ii) Complete the palindrome which is recognised by EcoRI. (iii) Name the enzyme that can link the two DNA fragments. 16. How do Darwin and de Vries differ in their views on the mechanism of evolution of life on earth? 17. State the use of the following enzymes/acids produced by the microbes: (i) Lipase (ii) Lactic acid (iii) Streptokinase (iv) Pectinase 18. (a) Name the green house gases that caused global warming. (b) Which of them has caused ozone hole and how?

Examination Papers
SECTION-C

43

19. Study the figure given:

e
d c

(i) Pick out and name the cells that undergo spermiogenesis. (ii) Name a and b cells. What is the difference between them with reference to the number of chromosomes? (iii) Pick out and name the motile cells. (iv) What is f cell? Mention its function. (v) Name the structure of which the given diagram is a part. 20. Define totipotency of a cell. List the requirements if the objective is to produce somaclones of a tomato plant on commercial scale. 21. (a) One of the codons on mRNA is AUG. Draw the structure of tRNA adapter molecule for this codon. (b) Name the RNA polymerase that transcribes tRNA in eukaryotes. (c) What is unique about the amino acid this tRNA binds with? 22. Study the graph given below:

Population density (N)

Time (t)

44

Xam idea BiologyXII (i) What does the curve a represent in the graph? What does K stand for? (ii) Which one of the two curves is considered a more realistic one for most of the animal population? (iii) Which curve would depict the population of a species of deer if there are no predators in the habitat? Why is it so? 23. A vector is engineered with three features which facilitate its cloning within the host cell. List the three features and explain each one of them. OR Why are restriction endonucleases so called? Explain their role as molecular scissors in recombinant DNA technology. 24. What is hnRNA? Explain the changes hnRNA undergoes during its processing to form mRNA. 25. Name the malarial parasite. Where do the gametocytes of this parasite develop? Give a flow chart of its life-cycle in this host. 26. (a) Expand BOD. (b) At a particular segment of a river near a sugar factory, the BOD is much higher than the normal level. What is it indicative of? What will happen to the living organisms in this part of the river? (c) Under what conditions will the BOD be lowered in the river? How will it affect the aquatic life? 27. Study the pedigree chart given below showing the inheritance pattern of a human trait and answer the questions that follow: I II

III (a) Give the genotype of the parents shown in generation I and of the son and daughter shown in generation II. (b) Give the genotype of the daughters shown in generation III. (c) Is the trait sex-linked or autosomal? Justify your answer.
SECTION-D

28. (a) Give a schematic representation of oogenesis in human female indicating the chromosomal number at each step. Mention at what stage of female life does each phase occur. (b) Where would you look for coleoptile and coleorhiza? What function do they perform? OR (a) Draw a labelled schematic representation of a mature embryo sac of an angiosperm. (b) Explain the role of ovarian hormones in inducing changes in the uterus during menstrual cycle. (c) What triggers release of oxytocin at the time of parturition?

Examination Papers

45

29. Explain the steps involved in the production of genetically engineered insulin. Why is insulin thus produced preferred to the one produced from non-human sources? OR (a) Why is Bacillus thuringiensis considered suitable for developing GM plants? (b) Explain how it has been used to develop GM crops. 30. What is semiconservative replication of DNA? Explain how it was experimentally proved. OR (a) A true breeding pea plant homozygous for axial violet flowers is crossed with another pea plant with terminal white flowers (aavv). Work out the cross to show the phenotypes and genotypes of F1 and F2 generations along with the ratios. (b) State the law that Mendel proposed on the basis of such a cross.

SETII
(Uncommon Questions to Set I) SECTION-A

3. High fever, loss of appetite, stomach pain and constipation are some of the symptoms seen in a patient. How would the doctor confirm that the patient is suffering from typhoid and not amoebiasis? 6. Given below is a schematic representation of a lac operon in the absence of an inducer. Identify a and b in it.
p i p o o b Repressor mRNA a y a

7. State the principle on which ELISA works. 8. Name the pioneer species: (i) on a bare rock (ii) in a water body
SECTION-B

9. Name the blank spaces a, b, c and d in the table given below: Name of the Drug (i) a (ii) Marijuana Plant Source Poppy plant c Organ System Affected b d

11. Why is Saheli considered to be an improved form of oral contraceptive for human females?

46

Xam idea BiologyXII 15. Give the sum total of all the allelic frequencies in a stable population. List any two factors which disturb the stability of a population. How does this disturbance affect the population?
SECTION-C

22. (a) What is micropropagation? Why are the plants produced by rnicropropagation called somaclones? (b) Name the technique by which healthy plants can be recovered from the diseased plants. 26. Study the graph below and answer the questions which follow:

Population density (N)

Time (t)

(i) The curve a is represented by the equation

dN = rN. What does r represent in the dt

equation and what is its importance? (ii) Which one of the two curves is considered a more realistic one for most of the animal population? (iii) Which curve would depict the population of a species of deer if there are no predators in the habitat? Why is it so?
SECTION-D

30. A true breeding pea plant, homozygous for inflated and green pods is crossed with another pea plant with constricted and yellow pods (ffgg). (i) Work out the cross to show the phenotypes and genotypes of F1 and F2 generations. Give the phenotype ratio of F2 generation. (ii) List the laws of Mendel which can be derived from such a cross. OR (i) Who explained the transforming principle in an organism? How did the scientist perform the experiment to explain this principle? (ii) How was the biochemical nature of the transforming material determined? Explain.

Examination Papers

47

SETIII
(Uncommon Questions to Set I and II) SECTION-A

3. Give the scientific name of the pathogen causing malignant malaria in humans. 6. What are a and b in the transcription unit represented below?
3' a 3' b

SECTIONB

9. How do normal cells get transformed into cancerous neoplastic cells? Mention the difference between viral oncogenes and cellular oncogenes. 10. Name the interaction in each of the following: (a) Cuscuta growing on a shoe flower plant. (b) Mycorrhizae living on the roots of higher plants. (c) Clown fish living among the tentacles of sea anemone. (d) Koel laying its eggs in crows nest. 11. How do pills act as contraceptives in human females? 12. State HardyWeinberg principle of genetic equilibrium. Knowing that genetic drift disturbs this equilibrium, mention what does this disturbance in genetic equilibrium lead to.
SECTIONC

26. Study the graph given below and answer the questions which follow:

Population density (N)

Time (t)

(i) The curve b is described by the following equation: dN K N = rN dt K What does K stand for in this equation? Mention its significance.

48

Xam idea BiologyXII (ii) Which one of the two curves is considered a more realistic one for most of the animal populations? (iii) Which curve would depict the population of a species of deer if there are no predators in the habitat? Why is it so? 27. Explain the advantages of inbreeding in cattle population. What effect does inbreeding depression have on cattle population and how is it overcome?
SECTIOND

30. What are transgenic animals? Explain any four ways in which such animals can be beneficial to humans. Or (a) What is a plasmid? (b) What is meant by ADA deficiency? How is gene therapy a solution to this problem? Why is it not a permanent cure?

Examination Papers

49

SOLUTIONS
SETI SECTIONA

1. Seed ferns. 2. Animals hibernate in unfavourable or stressful conditions when they are unable to migrate to avoid the stressful conditions. 3. Saccharum officinarum has thicker stem, higher sugar content and grows well in South India. 4. GEAC makes decisions regarding the validity of GM research. It also checks the safety of introducing GM organisms for public services. 5. Macrophages phagocytose and destroy microbes. 6. a is phosphate group b is purine (adenine/guanine) 7. Nile perch. 8. RNA interference in all eukaryotic organisms is a method of cellular defence.
SECTIONB

9. aFruit wall bScutellum cPlumule and radicle dPerisperm 10. The genotype of a purple flowered plant can be determined by conducting a test cross. Case I

If the F1 generation produces all purple flower the parent would be homozygous dominant, i.e., pp. Case II

50

Xam idea BiologyXII If the F1 generation produces purple and white flowers in 1:1 ratio, the parent would be heterozygous, i.e., Pp. OR Since the progeny have blood group O, the parents must be heterozygous. The possibilities would be as shown:

Thus, the F1 progeny can have all the four possible blood groups, i.e., A, B, AB and O. 11. (a) Commensalism (b) Commensalism (c) Competition (d) Mutualism 12. (i) They may get viral infections like AIDS and hepatitis B which are transferred through syringes. (ii) They may get addicted to drugs. 13. Grazing Food Chain: Grass Grasshopper Frog Snake Vulture or Grass Grasshopper Bird Snake Vulture Detritus Food Chain: Decaying plant matter Earthworm Bird Snake Vulture 14. Surgical methods are terminal and permanent methods which block the transport of gametes, thereby preventing conception. In males, a small part of the vas deferens is removed and tied up through a small incision on the scrotum. This is called vasectomy. 15. (i) aVector DNA bForeign DNA (ii) 5 GAATTC 3 3 CTTAAG 5 (iii) DNA ligase

Examination Papers 16. S.No. (i) (ii) Darwins Evolution de Vries Evolution

51

According to Darwin, evolution was According to de Vries, evolution occurred gradual (stepwise). in a single step (saltation). Variations and natural selection occurs Single step mutation caused speciation. through a number of generations and are responsible for speciation. Darwins variations are small and de Vries mutations are random and directional. directionless.

(iii) 17.

(i) Lipase: Used in detergent formulations and helps in removing oily stains from laundry. (ii) Lactic acid: Converts milk into curd. (iii) Streptokinase: Used as clot-buster for removing clots from blood vessels of patients who have undergone myocardial infarction. (iv) Pectinase: Used for clarifying bottled juices. 18. (i) CO2, CH4, N2O, chlorofluorocarbons or CFCs (ii) Ozone degradation has increased due to chlorofluorocarbons (CFCs). CFCs are refrigerants which react with UV in stratosphere to release chloride atoms. Chloride atoms act as catalyst to degrade ozone and release molecular oxygen. CFCs have permanent and continued effect as chloride atoms are not consumed.
SECTIONB

19.

(i) dSpermatids (ii) aSpermatogomium bPrimary spermatocyte They both are diploid having 46 chromosomes. (iii) eSpermatozoa (iv) fSertoli cells Provides nutrition to the germ cells. (v) Seminiferous tubule. 20. The capacity of a cell/explant to grow into a whole plant is called totipotency. Requirements: (i) Explant: Any part of a plant taken out and grown in a test tube. (ii) Nutrient medium: It must have carbon source (sucrose), inorganic salts, growth regulators (auxins, cytokinins, etc.), vitamins and amino acids. (iii) Suitable light and temperature conditions.

52 21. (a)
fMet

Xam idea BiologyXII

tRNA

U A A U 5'

C Anticodon G Codon Initiator tRNA

mRNA 3'

(b) RNA polymerase III. (c) Formyl-methionine is the initiator amino acid. 22. (i) a represents exponential growth curve. K represents carrying capacity. (ii) Curve b is considered more realistic. (iii) Curve a. In the absence of predators and unlimited resources, the populations grow exponentially. 23. (i) Origin of replication (ori) This is a DNA sequence that is responsible for initiating replication. Any piece of DNA when linked to this sequence can replicate within the host cells. Ori also controls the copy numbers of the linked DNA. (ii) Selectable marker It helps to select the host cells which contain the vector (transformants) and eliminate the non-transformants. Genes encoding resistance to antibiotics like ampicillin, chloramphenicol, tetracycline or kanamycin, are useful selectable markers for E. coli. (iii) Cloning sites To link the alien DNA, the vectors require very few (mostly single) recognition sites for the restriction enzymes. More than one recognition sites within the vector, can complicate the gene cloning as it will generate several fragments. Ligation of alien DNA can be carried out at a restriction site present in one of the two antibiotic resistance genes. OR Restriction endonucleases are called so because they restrict the growth of bacteriophages by recognising and cutting DNA at specific sites. Following are the functions of restriction endonucleases: n Every endonuclease inspects the entire DNA sequence for the palindromic recognition sequence.

Examination Papers
n n

53

On finding the palindrome, the endonuclease binds to the DNA. It cuts the opposite strands of DNA in the sugarphosphate backbone; a little away from the centre of the palindrome sites but between the same bases on both strands. n This results in the formation of single stranded overhanging stretches at the end of each strand are called sticky ends. n The sticky ends facilitate the action of the enzyme DNA ligase by readily forming hydrogen bonds with complementary strands. n In genetic engineering, DNA from different sources are cut with the same restriction enzymes so that both DNA fragments have same kind of sticky ends. n These sticky ends are complementary to each other and thus can be joined by DNA ligase (end-to-end). 24. hnRNA is the precursor of mRNA that is transcribed by RNA ploymerase II and is called heterogenous nuclear RNA. Changes: n The hnRNA undergoes two additional processes called capping and tailing. n In capping, an unusual nucleotide, methyl guanosine triphosphate, is added to the 5-end of hnRNA. n In tailing, adenylate residues (about 200300) are added at 3-end in a template independent manner. n Now the hnRNA undergoes a process where the introns are removed and exons are joined to form mRNA by the process called splicing. 25. Plasmodium sps. Gametocytes develop in red blood cells of humans. Life-cycle: Sporozoites are injected with the bite of Anopheles mosquito into the body. Sporozoites reach the liver through blood Parasite reproduces asexually in the liver cells, and by bursting the liver cells enter the blood. They enter the red blood cells Parasites reproduce asexually in red blood cells and by bursting them release haemozoin which causes cycles of fever Parasites enter new red blood cells Starts the sexual stages (gametocytes) in red blood cells. Stages in the life cycle of Plasmodium 26. (a) BODBiochemical Oxygen Demand

54

Xam idea BiologyXII (b) It indicates the addition of lot of organic matter; microorganisms involved in the biodegradation of organic matter in the water body consume a lot of oxygen and as a result, there is a sharp decline in the dissolved oxygen content downstream from the point of addition of effluent from the factory. This causes mortality of fish and other aquatic organisms. (c) When the amount of organic matter decreases and the microbes do not need oxygen for decomposition, the BOD decreases. Thus, aquatic organisms will start flourishing. 27. (a) Genotype of the parents in generation I: Male (Father) Aa Female (Mother) Aa Son (Generation II) Aa Daughter (Generation II) aa (b) Genotype of the daughters in generation IIIAa (c) It is an autosomal trait, because if the sex-linked trait has to appear in the daughter (generation II), the father must have it; put he does not show the trait and so it is not sex-linked.
SECTIOND

28. (a) Schematic representation of oogenesis:

(b) The shoot apex and few leaf primordia is enclosed in a hollow foliar structure in epicotyl region called coleoptile. Coleorhiza is an undifferentiated sheath that encloses the radicle and root cap. They both have protective function. Coleoptile protects the plumule and coleorhiza protects the radicle of the embryonal axis.

Examination Papers

55

29.Genetically engineered insulin: Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly, in 1983. It prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E.coli for production. The A and B chains produced were separated, extracted and combined by creating disulfide bonds to form human insulin. Since insulin produced from non-human sources causes allergy or other reactions to the foreign protein, genetically engineered insulin is preferred. OR (a) Some strains of Bacillus thuringiensis produce proteins that kill some insects like lepidopterans (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes). Bt toxins are initially inactive protoxins but after ingestion by the insect their inactive toxin becomes active due to the alkaline pH of the gut which solublise the crystals. The activated toxin binds to the surface of midgut epithelial cells thus creating pores which causes cell swelling and lysis, further leading to death of the insects. (b) Bacillus thuringiensis produces Cry protein. Cry protein producing gene is transferred to the plant to provide resistance against insect larvae. Man has developed several transgenic crops by introducing these genes from bacteria to crop plants such as Bt cotton, Bt corn, etc. 30. Watson and Crick in 1953 proposed a scheme that DNA replication was semiconservative. According to the scheme, the two parental strands separate and each strand acts as a template for synthesising a complementary strand over it. After completion of replication, each DNA had one parental strand and one newly synthesised strand. Experimental Proof for semi-conservative mode of DNA replication: n Matthew Meselson and Franklin Stahl grew E. coli in a medium containing 15NH4Cl (in which 15N is the heavy isotope of nitrogen) for many generations. n As a result, 15N got incorporated into newly synthesised DNA. n This heavy DNA can be differentiated from normal DNA by centrifugation in caesium chloride (CsCl) density gradient. n Then they transferred the cells into a medium with normal 14NH4Cl and took the samples at various definite time intervals as the cells multiplied. n The extracted DNAs were centrifuged and measured to get their densities. n The DNA extracted from the culture after one generation of transfer from the 15N medium to 14N medium (i.e., after 20 minutes) showed an intermediate hybrid density. The DNA extracted from culture after two generations (i.e., after 40 minutes) showed equal amount of light DNA and hybrid DNA. OR Parents
Genotypes Gametes F1 generation

Axial, violet flowers

AAVV AV

Terminal, white flowers

aavv av AaVv (Axial, Violet flowers)

56

Xam idea BiologyXII

On selfing Gametes AV

AaVv Av aV av

AV Av

AaVv aV av

AV F2 generation AV AAVV Axial, Violet Av AAVv Axial, Violet aV AaVV Axial, Violet av AaVv Axial, Violet

Av AAVv Axial, Violet AAvv Axial, White AaVv Axial, Violet Aavv Axial, White

aV AaVV Axial, Violet AaVv Axial, Violet aaVV

av AaVv Axial, Violet Aavv Axial, White aaVv

Terminal, Violet Terminal, Violet aaVv aavv

Terminal, Violet Terminal, white

(a) Phenotype of F1 generation: All axial, violet flowers: Genotype of F1 generation: AaVv. (b) Phenotypic ratio of F2 generation: Axial violet flowers 9 : Axial white flowers 3 : Terminal violet flowers 3 : Terminal white flowers 1

(c) Law of independent assortment: This law states that the different factors or allelomorphic pair in gametes and zygotes assort themselves and seggregate independently of one another.

SET II
SECTIONA

3. Typhoid would be confirmed by Widal Test. 6. aRepressor bRepressor bound to the operator and prevents transcription of structural genes. 7. ELISA is based on antigenantibody reaction. 8. (i) Lichens (ii) Phytoplanktons.
SECTIONB

9. aMorphine bCentral nervous system cCannabis sativa dCardiovascular system. 11. Saheli contains a non-steroidal preparation and it is once-a-week pill. It has very few side-effects but high contraceptive value.

Examination Papers

57

15. Gene pool is the sum total of all allelic frequencies. The following two factors disturb the stability of a population: (i) Gene migration or gene flow: When individuals migrate to another place or population, new genes or alleles are added to new population and are lost from old population, in turn changing the frequencies. (ii) Mutation: Advantageous mutations lead to new phenotypes and over few generations, result in speciation.
SECTIONC

22. (a) Micropropagation is the method of growing a number of plants through tissue culture. Since tissue culture involves only mitotic divisions, the plantlets formed are genetically identical and hence are called somaclones. (b) Meristem culture. 26. (i) r represents the intrinsic rate of natural increase. It is an important parameter for assessing the impact of any abiotic or biotic factors on the population growth. (ii) Curve b is more realistic. (iii) Curve a. When the predators are absent, the prey population grows exponentially.
SECTIOND

30.

(i)
Parents Genotypes Gametes F1 generation On selfing Gametes FG FfGg Fg fG fg FG Fg (Inflated, green pods) FFGG FG (Constricted, yellow pods) ffgg fg FfGg (All Inflated green pods) FfGg fG fg

FG F2 generation: FG FFGG Inflated, Green FFGg Fg Inflated, Green FfGG fG Inflated, Green FfGg fg Inflated, Green

Fg FFGg Inflated, Green FFgg Inflated, Yellow FfGg Inflated, Green Ffgg Inflated, Yellow

fG FfGG Inflated, Green FfGg Inflated, Green ffGG Constricted, Green ffGg Constricted, Green

fg FfGg Inflated, Green Ffgg Inflated, Yellow ffGg Constricted, Green ffgg Constricted, Yellow

58
F2 phenotypic ratio: Inflated Green Pods 9 : : Inflated Yellow Pods 3 : : Constricted Green Pods 3 : :

Xam idea BiologyXII

Constricted Yellow Pods 1

(ii) Mendels Law of Independent Assortment: This law states the different factors or allelomorphic pair in gametes and zygotes assort themselves and segregate independently of one another. OR (i) Frederick Griffith (1928) conducted experiments with Streptococcus pneumoniae (bacterium causing pneumonia). n He observed two strains of this bacteriumone forming smooth shiny colonies (S-type) with capsule, while other forming rough colonies (R-type) without capsule. n When live S-type cells were injected into mice, they died due to pneumonia. When live R-type cells were injected into mice, they survived. n When heat-killed S-type cells were injected into mice, they survived and there were no symptoms of pneumonia. n When heat-killed S-type cells were mixed with live R-type cells and injected into mice, they died due to unexpected symptoms of pneumonia. He concluded that heat-killed S-type bacteria caused a transformation of the R-type bacteria into S-type bacteria. (ii) Oswald Avery, Colin MacLeod and Maclyn McCarty repeated Griffiths experiment in an in vitro system in order to determine biochemical nature of transforming principle. They concluded that DNA is the hereditary material.

SET III
SECTIONA

3. Plasmodium falciparum. 6. aPromoter bTerminator

SECTIONB

9. Normal cells are transformed into cancerous cells due to various physical, chemical and biological agents called carcinogens. Cellular oncogenes (c-onc) or proto-oncogenes are present in inactivated state but under certain conditions (like mutation) get transformed to cancer-causing oncogenes. Whereas viral oncogenes are cancer-causing genes of virus. 10. (a) (b) (c) (d) Parasitism Mutualism Commensalism Brood parasitism

Examination Papers

59

11. They inhibit ovulation and implantation as well as other quality of cervical mucus to prevent entry of sperms. 12. HardyWeinberg principle states that allele frequencies in a population are stable and remain constant from generation to generation. n Genetic drift refers to change in allele frequencies of a population occurring by chance.
n

The change in allele frequency may be so different that the population becomes a different species.
SECTIONC

26.

(i) K stands for carrying capacity. Carrying capacity is defined as the maximum number of individuals of a population that can be sustained by the given habitat/environment. (ii) Curve b is more realistic. (iii) Curve a. When the predators are absent, the prey population grows exponentially. 27. Advantages of inbreeding: (i) Inbreeding increases and evolves a pure line. (ii) Recessive genes are exposed by inbreeding which are then eliminated by selection. (iii) Superior genes can be accumulated by inbreeding by eliminating undesirable genes. (iv) By selection at every step, productivity of inbred population is increased. Inbreeding depression reduces the fertility and over productivity of animals. In order to overcome the cattle from inbreeding depression, selected animals of the breeding population should be mated with unrelated superior animals of the same breed. This helps in restoring the fertility and yield in the cattle.
SECTIOND

30. Animals whose DNA is manipulated to possess and express an extra (foreign) gene are known as transgenic animals. Following are the common ways in which they are beneficial: (i) Study of normal physiology and development Useful to study gene regulation, their effect on the normal functions of the body and its development. For example, study of complex growth factors like insulin-like growth factor. (ii) Study of disease Study of genes which are responsible for diseases in human and their treatment. Transgenic models have been developed for many human diseases like cancer, cystic fibrosis, rheumatoid arthritis and Alzheimers disease. (iii) Biological products Useful biological products can be produced by introducing into transgenic animals the portion of DNA (or genes) which codes for a particular product. For example, human protein (a-1-antitrypsin) is used to treat emphysema. (iv) Vaccine safety Transgenic mice are developed to test safety of vaccines before being used on humans. For example, polio vaccine.

60 (v) Chemical safety testing

Xam idea BiologyXII

Transgenic animals are made to carry genes which make them more sensitive to the toxic substances than non-transgenic animals. On exposing to the toxic substances, their effects are studied in less time. OR (a) Plasmid is a circular extra-chromosomal DNA molecule present in a bacterial cell, which replicates autonomously along with bacterial chromosomal DNA. (b) ADA is adenosine deaminase deficiency. ADA is caused due to deletion of gene for adenosine deaminase. In some cases, it can be cured by bone marrow transplantation and enzyme replacement therapy but it is not fully curative. Lymphocytes from patients blood were grown in a culture and functional ADA cDNA was introduced in these lymphocytes using a retroviral vector. The lymphocytes were transferred into the patients body. Periodic infusion of such genetically engineered lymphocytes is done because these cells are mortal. For permanent cure, gene isolated from the bone marrow cells producing ADA at early embryonic stage can be a possible cure.

EXAMINATION PAPERS 2009 CBSE (Delhi) SETI

Time allowed: 3 hours Maximum marks: 70

General Instructions: (I) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. Section A contains 8 questions of one mark each, Section B is of 10 questions of two marks each, Section C is of 9 questions of three marks each and Section D is of 3 questions of five marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.
SECTIONA

1. Why hnRNA is required to undergo splicing? 2. The microscopic pollen grains of the past are obtained as fossils. Mention the characteristic of the pollen grains that makes it happen. 3. How does colostrum provide initial protection against diseases to new born infants? Give one reason. 4.
Crustaceans b Other animal groups

5. 6. 7. 8.

Name the unlablelled areas a and b of the pie chart (given above) representing the global biodiversity of invertebrates showing their proportionate number of species of major taxa. Mention the type of evolution that has brought the similarity as seen in potato tuber and sweet potato. Name the group of organisms and the substrate they act on to produce biogas. Mention the pollinating agent of an inflorescence of small dull coloured flowers with well exposed stamens and large feathery stigma. Give any one characteristic of pollen grains produced by such flowers. Name the organism commercially used for the production of single cell protein.
SECTIONB

9. Explain the contribution of Thermus aquaticus in the amplification of a gene of interest.

62 10.
a b

Xam idea BiologyXII

(i) What does the above diagram illustrate? (ii) Name the parts labelled a and b. (iii) Name the type of cells that produce this molecule. 11. Banana is a parthenocarpic fruit whereas oranges show polyembryony. How are they different from each other with respect to seeds? OR Where are fimbriae present in a human female reproductive system? Give their function. 12. How is the translation of mRNA terminated? Explain. 13. Explain accelerated eutrophication. Mention any two consequences of this phenomenon. 14. List the specific symptoms of amoebiasis. Name the causative organism. 15. A crane had DDT level as 5 ppm in its body. What would happen to the population of such birds? Explain giving reasons. 16. Describe the responsibility of GEAC, set up by the Indian Government. 17. During the secondary treatment of the primary effluent how does the significant decrease in BOD occur? 18. Study the figure given below and answer the questions:
i p o z y a

mRNA

lac mRNA

Enzymes

(a) How does the repressor molecule get inactivated? (b) When does the transcription of lac mRNA stop? (c) Name the enzyme transcribed by the gene z.

Examination Papers
SECTION C

63

19. Name the pioneer species on a bare rock. How do they help in establishing the next type of vegetation? Mention the type of climax community that will ultimately get established. 20. Study the figure given below and answer the questions that follow:
Inner cell mass

(a) Name the stage of human embryo the figure represents. (b) Identify a in the figure and mention its function. (c) Mention the fate of the inner cell mass after implantation in the uterus. (d) Where are the stem cells located in this embryo? 21. Give the scientific name of the parasite that causes malignant malaria in humans. At what stage does this parasite enter the human body? Trace its life cycle in human body. 22. Draw a labelled schematic sketch of replication fork of DNA. Explain the role of the enzymes involved in DNA replication. 23. Explain the causes of global warming. Why is it a warning to mankind? 24. Haemophilia is a sex linked recessive disorder of humans. The pedigree chart given below shows the inheritance of haemophilia in one family. Study the pattern of inheritance and answer the questions given.
1 2

10

11

12

13

14

15

(a) Give all the possible genotypes of the members 4, 5 and 6 in the pedigree chart. (b) A blood test shows that the individual 14 is a carrier of haemophilia. The member numbered 15 has recently married the member numbered 14. What is the probability that their first child will be a haemophilic male? OR Inheritance pattern of ABO blood group in humans shows dominance, co-dominance and multiple allelism. Explain each concept with the help of blood group genotypes.

64 25.
Tasmanian wolf

Xam idea BiologyXII

Sugar glider Tiger cat

Marsupial mole Marsupial radiation

Banded anteater Koala Marsupial rat

Bandicoot

Wombat

Kangaroo

(a) Mention the specific geographical region where these organisms are found. (b) Name and explain the phenomenon that has resulted in the evolution of such diverse species in the region. (c) Explain giving reasons the existence of placental wolf and Tasmanian wolf sharing the same habitat. 26.
b Cla I c

Pvu I Pst I a pBR322 d

BamH I

Sal I ori rop

Pvu II

(a) Identify the selectable markers in the diagram of E. coli vector shown above.

Examination Papers

65

(b) How is the coding sequence of a-galactosidase considered a better marker than the ones identified by you in the diagram? Explain. 27. Construct an ideal pyramid of energy when 1,000,000 joules of sunlight is available. Label all its trophic levels.
SECTION D

28. Explain with the help of a diagram the development of a mature embryo sac from a megaspore mother cell in angiosperm. OR Study the following flow chart. Name the hormones involved at each stage. Explain their functions. Hypothalamus Pituitary Testes Sperms 29. (a) Explain the experiment performed by Griffith on Streptococcus pneumoniae. What did he conclude from this experiment? (b) Name the three scientists who followed up Griffiths experiments. (c) What did they conclude and how? OR Two blood samples A and B picked up from the crime scene were handed over to the forensic department for genetic fingerprinting. Describe how the technique of genetic fingerprinting is carried out. How will it be confirmed whether the samples belonged to the same individual or to two different individuals? 30. One of the main objectives of biotechnology is to minimise the use of insecticides on cultivated crops. Explain with the help of a suitable example how insect resistant crops have been developed using techniques of biotechnology. OR (a) How is mature insulin different from proinsulin secreted by pancreas in humans? (b) Explain how was human functional insulin produced using rDNA technology. (c) Why is the functional insulin thus produced considered better than the ones used earlier by diabetic patients?

SETII
(Uncommon Questions to Set I) SECTIONA

4. Why are the wings of a butterfly and of a bat called analogous? 8. How are the two following varieties of sugarcane different from each other? (i) Saccharum barberi (ii) Saccharum officinarum

66
SECTIONB

Xam idea BiologyXII

11. Name the cell from which the endosperm of coconut develops. Give the characteristic features of endosperm of coconut. OR How does Cu-T act as an effective contraceptive for human females? 13. Nematode-specific genes are introduced into the tobacco plants using Agrobacterium vectors to develop resistance in tobacco plants against nematodes. Explain the events that occur in tobacco plant to develop resistance. 15. Egrets are often seen along with grazing cattle. How do you refer to this interaction? Give a reason for this association.
SECTIONC

24. Mention the target cells of luteinising hormone in human males and females. Explain the effect and the changes which the hormone induces in each case.
SECTIOND

29. (a) Draw a diagrammatic sectional view of human ovary showing different stages of oogenesis along with corpus luteum. (b) Where is morula formed in humans? Explain the process of its development from zygote. OR How does the pollen mother cell develop into a mature pollen grain? Illustrate the stages with labelled diagrams. 30. How did Hershey and Chase prove that DNA is the hereditary material? Explain their experiment with suitable diagrams. OR Inheritance pattern of flower colour in garden pea plant and snapdragon differs. Why is this difference observed? Explain showing the crosses up to F2 generation.

SETIII
(Uncommon Questions to Set I and II) SECTIONA

3. Some allergens trigger sneezing and wheezing in human beings. What causes this type of response by the body? 5. Are the thorn of Bougainvillea and tendril of Cucurbita homologous or analogous? What type of evolution has brought such a similarity in them? 6. Mention the two additional processings which hnRNA needs to undergo after splicing so as to become functional. 8. Which of the following is a free living bacteria that can fix nitrogen in the soil? Spirulina, Azospirillum, Sonalika

Examination Papers
SECTIONB

67

10. Name the muscular and the glandular layers of human uterus. Which one of these layers undergoes cyclic changes during menstrual cycle? Name the hormone essential for the maintenance of this layer. OR Name the hormonal composition of the oral contraceptive used by human females. Explain how does it act as a contraceptive. 14. (a) What is r in the population equation given: dN/dt = rN (b) How does the increase and the decrease in the value of r affect the population size?
SECTIONC

19. (a) Name the respective forms in which the malarial parasite gains entry into (i) Human body and (ii) Body of female Anopheles. (b) Name the hosts where the sexual and the asexual reproductions of malarial parasites occur respectively. (c) Name the toxin responsible for the appearance of symptoms of malaria in humans. Why do these symptoms occur periodically?
SECTIOND

28. (a) What did Meselson and Stahl observe when (i) they cultured E. coli in a medium containing 15NH4Cl for a few generations and centrifuged the content? (ii) they transferred one such bacterium to the normal medium of NH4Cl and cultured for 2 generations? (b) What did Meselson and Stahl conclude from this experiment? Explain with the help of diagrams. (c) Which is the first genetic material? Give reasons in support of your answer OR You are given a red flower-bearing pea plant and a red flower-bearing snapdragon plant. How would you find the genotypes of these two plants with respect to the colour of the flower? Explain with the help of crosses. Comment upon the pattern of inheritance seen in these two plants. Draw a labelled diagram of a mature embryo sac. Why does a pollen grain possess two male gametes? Explain. OR Draw a schematic diagram of a human sperm and label the cellular components. Give the functions of any three parts. Where are the sperm heads found embedded to survive after spermatogenesis?

29. (a) (b) (a) (b)

68

Xam idea BiologyXII

SOLUTIONS
Delhi -2009 (Set I, II, III) SETI SECTIONA

1. hnRNA undergoes splicing in order to remove introns which are intervening or non-coding sequences and exons are joined to form functional mRNA. 2. The exine of pollen grains have an outermost hard layer composed of a chemical, sporopollenin. It is highly resistant to high temperature, strong acids and alkali. 3. Colostrum provides protection to new born babies due to the presence of several antibodies essential to develop resistance. 4. (a) Insects (b) Molluscs. 5. Convergent evolution. 6. OrganismsMethanogens SubstrateCellulosic material/cow dung/agriculture waste. 7. Pollinating agent is wind. Pollen grains are light, dry and non-sticky. 8. Spirulina.
SECTIONB

9. Thermus aquaticus produces a thermostable DNA polymerase enzyme which remains active during the high temperature induced denaturation and separation of the double stranded DNA during PCR (polymerase chain reaction). 10. (i) An antibody molecule (ii) aAntigen binding site bLight chain (iii) B-lymphocytes (B-cells). 11. Banana develops from an ovary without fertilisation having non-viable seeds so it is called parthenocarpic fruit. An orange contain seeds with more than one embryo thus, it shows polyembryony. OR The fimbriae are the finger-like projections present on the edges of infundibulum (fallopian tubes). They help in collection of ovum after ovulation. 12. mRNA contains a stop codon (UAA, UAG, UGA) which do not code for any amino acid and are not identified by any tRNAs. As a result, the synthesis of polypeptide chain stops and the polypeptide chain is released from the ribosome. 13. Accelerated eutrophication is nutrient enrichment of water bodies due to human activities like passage of sewage.

Examination Papers

69

Its consequences are: (i) Large amount of nutrients in waters causes excessive growth of planktonic (free-floating) algae, called algal bloom which impart characteristic colour to water bodies. (ii) Depletion of oxygen content of water leading to the death of the aquatic organisms. 14. The symptoms of amoebiasis are: (i) Abdominal pain and cramps (ii) Stools with excess mucus and blood clots (iii) Constipation. Causative organismEntamoeba histolytica. 15. The high concentration of DDT (5 ppm) in cranes disturb their calcium metabolism which causes thinning of eggshell and their premature breaking, thereby causing decline in the bird populations. 16. GEAC (Genetic Engineering Approval Committee) make decisions regarding the validity of GM research. It also checks the safety of introducing GM-organisms for public services for their large scale use. 17. During secondary treatment of primary effluents, vigorous growth of useful aerobic microbes into flocs occur when it is agitated mechanically and air is pumped into it in a large aeration tank. These microbes while growing consume major part of the organic matter in the effluent. This significantly reduces BOD. 18. (a) When the inducer comes in contact with repressor, repressor is inactivated. (b) When lactose is lacking or absent, the transcription of lac mRNA stops. (c) b-galactosidase.
SECTIONC

19. The pioneer species on a bare rock are usually lichens. The lichens secrete acids to dissolve rocks which help in weathering and formation of small amount of soil. Now these suitable conditions help in establishing small plants like bryophytes. They are, with time, succeeded by bigger plants, and after several more stages, ultimately a climax community is formed. The climax community is forest. 20. (a) Blastocyst (b) a is trophoblast. The trophoblast layer gets attached to endometrium and later form extra embryonic membrane namely chorionic villi. (c) The inner cell mass differentiates into an outer layer ectoderm and a inner layer endoderm. (d) Inner cell mass. 21. Scientific name of the parasite that causes malignant malaria in human is Plasmodium falciparum. Parasite enters the human body as sporozoite.

70 Life cycle in human body:

Xam idea BiologyXII

Sporozoites enters into the body (human) by biting of a female Anopheles mosquito. Sporozoites enters liver cells through blood The parasite reproduces asexually in liver cells, and through bursting of host liver cells enter into blood. In red blood cells parasite reproduces asexually, bursting the red blood cells followed by release of haemozoin thereby causing cycles of fever and other symptoms. Released parasite enters new RBCs thereby infecting them. 22.

Enzymes involved in DNA replication are: (i) DNA-dependent DNA polymerase catalyses the polymerisation of polynucleotides in a very short time only in 5 - 3 direction with accuracy. (ii) DNA ligase joins the discontinuously synthesised short segments called Okazaki fragments on one of the template strands. 23. Causes of global warming: (i) Deforestation (ii) Rise in the concentration of greenhouse gases (CO 2 , CH 4 , CFCs, N 2 O). (iii) Burning of fossil fuels (iv) Rise in industrial wastes and pollutants.

Examination Papers

71

Global warming is a warning to mankind because: (i) Rise in temperature is leading to increased melting of polar ice-caps as well as of other places like the Himalayan snow caps. This will result in a rise in sea level that can submerge many coastal areas. (ii) Deleterious changes in the environment results in odd weather and climate changes, e.g., El Nino effect. 24. (a) Genotypes of member 4 XX or XX h Genotypes of member 5 X h Y Genotypes of member 6 XY (b) The probability of first child to be a haemophilic male is 25%. OR Dominance: It is the phenomenon where one allele expresses itself exclusive of the presence of other allele. The alleles I A and I B both are dominant over allele i as I A and I B forms antigen A and antigen B respectively but i does not form any antigen. Co-dominance: It is the phenomenon when the F1 generation resembles both the parents, and both the parental characters are expressed simultaneously. Both the alleles I A and I B are co-dominant as both of them are able to express themselves in the presence of each other in blood group AB (I A I B ) by forming antigen A and B. Multiple allelism: It is the phenomenon of occurrence of a gene in more than two allelic form on the same locus. The ABO blood group in humans are determined by three different allelic forms I A , I B and i. 25. (a) Australia. (b) Adaptive radiation (Divergent evolution). The process of evolution of different species in a given geographical area starting from a point and literally radiating to other areas of geography (habitats) is called adaptive radiation. It is the development of different functional structures from a common ancestral form. (c) Placental wolf and Tasmanian wolf share similar habitat due to convergent evolution and evolved into unrelated group of organisms. 26. (a) aAmpicillin resistance dTetracycline. (b) The insertion of rDNA into the coding sequence of an enzyme a-galactosidase leads to the inactivation of the enzyme called insertional inactivation. The recombinants do not produce a blue coloured colonies in the presence of chromogenic substrate while the non-recombinants produce a blue colour. Thus, coding sequence of a-galactosidase is a better marker. 27.
Tertiary consumers Secondary consumers 1,000,000 J of energy of sunlight Primary consumers 10 J

100 J

1000 J

Producers

10,000 J

72
SECTIOND

Xam idea BiologyXII

28.
Micropylar end Nucellus Micropylar end Nucellus Megaspore tetrad Micropylar end

Megaspore mother cell

Megaspore dyad

(a) Micropylar end Chalazal end Antipodals Synergids

Egg Central cell 2 polar nuclei Polar nuclei Central cell Egg Synergids

(b)

Antipodals

Micropylar end (c)

Filiform apparatus

(a) Parts of the ovule showing a large megaspore mother cell, a dyad and a tetrad of megaspores; (b) 2, 4 and 8-nucleate stages of embryo sac and a mature embryo sac; (c) A diagrammatic representation of the mature embryo sac.

OR Hypothalamus GnRH Pituitary LH and FSH Testes Androgen (Testosterone) Sperms

Examination Papers

73

29.

Hypothalamus secretes gonadotropin releasing hormone (GnRH) which stimulates the anterior lobe of pituitary gland to secrete LH (Luteinising hormone) and FSH (Follicle stimulating hormone). LH stimulates Leydig cells and causes the secretion of testosterone from the testes. FSH stimulates Sertoli cells of the testes to secrete an androgen-binding protein (ABP) that concentrates testosterone in seminiferous tubules. It also causes the secretion of some factors which help in spermiogenesis. (a) Frederick Griffith (1928) conducted experiments with Streptococcus pneumoniae (bacterium causing pneumonia). He observed two strains of this bacteriumone forming smooth shiny colonies (S-type) with capsule, while other forming rough colonies (R-type) without capsule. When live S-type cells were injected into mice, they died due to pneumonia. When live R-type cells were injected into mice, they survived. When heat-killed S-type cells were injected into mice, they survived and there were no symptoms of pneumonia. When heat-killed S-type cells were mixed with live R-type cells and injected into mice, they died due to unexpected symptoms of pneumonia. He concluded that heat-killed S-type bacteria caused a transformation of the R-type bacteria into S-type bacteria but he was not able to understand the cause of this bacterial transformation. (b) Oswald Avery, Colin MacLeod and Maclyn McCarty. (c) They concluded that DNA is the hereditary material. They purified proteins, DNA and RNA from the heat-killed S cells to check which one transformed live R cells into S cells. They discovered that DNA alone from S bacteria caused R bacteria to become transformed. They also discovered that protein-digesting enzymes (proteases) and RNA-digesting enzymes (RNases) did not affect transformation, so the transforming substance was not a protein or RNA. Digestion with DNase inhibit transformation, showing that DNA caused the transformation. OR DNA Fingerprinting: This technique was discovered by Alec Jeffreys in 1985. The technique involves following steps: (i) Isolation and extraction of DNA from the cell by centrifugation. (ii) By the help of enzyme restriction endonuclease, DNA molecules are digested. The fragment also contains VNTRs. (iii) The small DNA fragments are separated through gel electrophoresis set-up that contain agarose polymer gel. (iv) The separated DNA fragments are transferred from electrophoresis plate to synthetic membranes like nitrocellulose or nylon membrane sheet called Southern blotting. (v) The DNA probes are added which target a specific nucleotide sequence which is complementary to them and this process is called hybridisation. (vi) The nylon membrane is exposed to an X-ray film and dark orange coloured bands developed at sites where probes have bound to the DNA fragments. This is known as autoradiography. On comparing the DNA prints of blood samples A and B, it can be confirmed that the blood sample picked up from the crime scene belongs to the same individual or to two different individuals.

74

Xam idea BiologyXII 30. By using the techniques of biotechnology, insect resistant crops have been developed. Some strains of Bacillus thuringiensis (Bt) produce protein crystals which contain a toxic insecticidal protein that kill some insects like lepidopterans, coleopterans and dipterans, etc. Specific Bt toxin genes were isolated from Bacillus thuringiensis and cloned in bacteria. Specific Bt toxin genes obtained from Bacillus thuringiensis are used in several crop plants like cotton by providing them resistance from insects. Bt toxins are insect-group specific coded by a gene called cry which are of various typesproteins encoded by the genes cryIAc and cryIIAb control the cotton bollworms, that of cryIAb controls corn borer. OR (a) The proinsulin has an extra stretch called the C peptide along with two short polypeptide chains A and B while the mature insulin has only two polypeptide chains A and B. (b) From the human cell, DNA containing insulin gene is isolated. The two DNA sequences corresponding to A and B, chains of human insulin is introduced into the plasmids of E. coli to produce insulin chains A and B. The extracted chain A and B were combined by creating disulfide bonds to form human insulin (humulin). (c) The insulin prepared by rDNA technology does not produce sensitive allergic reactions and immunological reactions whereas those used earlier produced allergic reactions and other complications to the foreign protein as earlier extracted from pancreas of slaughtered cattle or pigs.

SETII
SECTIONA

4. The wings of a butterfly and bat are called analogous organs because they are similar in function and differ in their structural details and origin. 8. (i) Saccharum barberi: It has poor sugar content and yield. (ii) Saccharum officinarum: It has higher sugar contents and stems are thicker.
SECTIONB

11. The endosperm of coconut develops from the primary endosperm cell. Characteristic features of endosperm of coconut are: (i) The water of coconut is free-nuclear endosperm made up of thousands of free nuclei. (ii) The surrounding white kernel of coconut is the cellular endosperm. OR CuT is an ideal contraceptive device for human females because: (i) Cu ions released suppress sperm motility and fertilising capacity of sperms. (ii) It increases phagocytosis of sperms within the uterus. 13. Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant. The introduction of DNA was such that it produced sense and anti-sense RNA in the host cells. Both the RNAs being complementary to each other form double stranded RNA (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. The parasite could not survive in a transgenic host expressing specific interfering RNA. Thus, transgenic plant is protected from the parasite.

Examination Papers

75

15. The interaction between them is called commensalism. Egrets always forage close to where the cattle are grazing because the cattle, as they move, stir up and flush out insects from the vegetation which otherwise might be difficult for the egrets to find and catch.
SECTIONC

24. The target cells of luteinising hormone in males are the Leydig cells and in females are the mature growing follicles. LH in males stimulates the Leydig cells (interstitial cells) of testes to synthesise and secrete androgens which in turn stimulate the process of spermatogenesis. LH in females stimulate the ovulation (release of ovum), transformation of Graafian follicle into corpus luteum to secrete progesterone which prepares the endometrium to receive and implant blastocyst.
SECTIOND

29. (a)
Blood vessels

Primary follicle

Tertiary follicle Showing antrum Graafian follicle

Ovum Corpus Iuteum Diagrammatic section view of ovary

(b) Morula is formed in the upper portion of oviduct, i.e., isthmus. The haploid nucleus of the sperms and that of the ovum fuse together to form a diploid zygote. Zygote divides rapidly as it moves through the isthmus of the oviduct by mitotic division called cleavage and as a result 2, 4, 8, 16 daughter cells are produced which are termed as blastomeres. The embryo with 8-16 blastomeres is called a morula. OR Pollen mother cells undergo meiosis to form a microspore tetrad (microspores arranged in a cluster of four cells) by the process called microsporogenesis. The microspores dissociates from each other and develop into pollen grains. The protoplast (pollen grain) then divides mitotically to form two unequal cells the bigger vegetative cell and smaller generative cell.
Vegetative cell Generative cell Asymmetric spindle

Vacuoles Nucleus

(b) (a) Enlarged view of a pollen grain tetrad; (b) Stages of a microspore maturing into a pollen grain.

(a)

76

Xam idea BiologyXII 30. Procedure: (i) Hershey and Chase grew some bacteriophage virus on a medium that contained radioactive phosphorus (32P) and some in another medium with radioactive sulphur (35S). (ii) Viruses grown in the presence of radioactive phosphorus (32P) contained radioactive DNA. (iii) Similar viruses grown in presence of radioactive sulphur (35S) contained radioactive protein. (iv) Both the radioactive virus types were allowed to infect E. coli separately. (v) Soon after infection, the bacterial cells were gently agitated in blender to remove viral coats from the bacteria. (vi) The culture was also centrifuged to separate the viral particle from the bacterial cell. Observations and Conclusions: (i) Only radioactive 32P was found to be associated with the bacterial cell, whereas radioactive 35S was only found in surrounding medium and not in the bacterial cell. (ii) This indicates that only DNA and not protein coat entered the bacterial cell. (iii) This proves that DNA is the genetic material which is passed from virus to bacteria and not protein.
Bacteriophage Radioactive (35 S) labelled protein capsule Radioactive (32 P) labelled DNA

1. Infection

2. Blending

3. Centrifugation No Radioactive (35 S) detected in cells + Radioactive (35 S) detected in supernatant Radioactive (32 P) detected in cells + No Radioactivity detected in supernatant

The Hershey-Chase experiment

Examination Papers

77

OR Inheritance pattern of flower colour in garden pea exhibits principle of dominance whereas in snapdragon incomplete dominance is exhibited. Inheritance of flower colour in garden pea: Parents Purple flower White flower Genotypes PP pp Gametes P p

F 1 generation F2 generation On selfing P p Pp P PP

Pp

(purple flowers)

Pp p Pp (Purple flower) pp (White flower)

(Purple flower) Pp (Purple flower) Phenotypic ratio 3: 1 Genotypic ratio 1: 2: 1. Inheritance of flower colour in snapdragon Parents Red flower Genotypes RR Gametes R

White flower rr r

F 1 generation On selfing

Rr R RR R r (Red flower) Rr (Pink flower)

Rr (pink flowers) Rr r Rr (Pink flower) rr (White flower)

Phenotypic ratio 1: 2: 1 Genotypic ratio 1: 2: 1.

78

Xam idea BiologyXII

SETIII
SECTIONA

3. The exaggerated response of the immune systems to certain antigens (allergens) present in the environment is the cause. 5. They are homologous organ. Divergent evolution has brought such a similarity in them. 6. Capping and tailing. 8. Azospirillum.
SECTIONB

10. The muscular layer of uterus is myometrium (middle thick layer of smooth muscle) and the glandular layer that lines the uterine cavity is endometrium (innermost). The endometrium undergoes cyclic changes during the menstrual cycle. Progesterone is the hormone essential for the maintenance of the endometrium. OR Oral contraceptive of female consists of either progestogen or progesteroneestrogen combination. They are taken as pills that inhibit ovulation and implantation. 14. (a) 'r is called intrinsic rate of natural increase. (b) Population size increases with the increase in r and it decreases with the decrease of r.
SECTIONC

19. (a) (i) Sporozoite (ii) Gametocytes. (b) Sexual reproduction occurs in mosquito, and the asexual reproduction takes place in human body. (c) Haemozoin. Parasites after entering the fresh RBCs take 48 to 72 hours to complete the erythrocytic cycle. Then they burst to release toxic substance called haemozoin and the symptoms like chill and high fever occurs periodically.
SECTIOND

28. (a) (i) Meselson and Stahl observed that in the E. coli bacterium the DNA becomes completely labelled with N 15 medium by centrifugation for few generations. (ii) After two generations density changed and showed equal amount of light DNA (N14) and dark hybrid DNA (N 14 N 15 ). (b) They concluded that DNA replicates semi-conservatively.

Examination Papers
Generation I
15

79
Generation II
14

N-DNA

14

N-DNA

N-DNA N-DNA N-DNA N-DNA

15

N-DNA 20 min 40 min

15

14

Gravitational force

14

15

N15 N Heavy

14

N15 N Hybrid

14

N14 N

14

N15 N Hybrid

Light

Meselson and Stahls Experiment (Separation of DNA by centrifugation)

(c) RNA is the first genetic material. Reasons: (i) RNA is highly reactive, and acts as a catalyst as well as a genetic material. (ii) Essential life processes such as metabolism, translation and splicing evolved around RNA. OR A test cross is required to find out the genotype of both the plant. (A) Garden pea (Homozygous red) Genotypes RR rr Gametes R r

F1 generation (Heterozygous red) Genotypes Gametes R Rr r

Rr

(red flowers)

rr r

F1 generation

Rr

rr White flower White flower rr r (Pink flowers)

Red flower (B) In snapdragon: Red flower Genotypes RR Gametes R

Rr

80 29. (a) A diagrammatic representation of the mature embryo sac.

Chalazal end Antipodals

Xam idea BiologyXII

Polar nuclei Central cell Egg Synergids

Micropylar end

Filiform apparatus

(b) Pollen grains possess two male gametes as it is required for double fertilisation in most angiosperms. Out of two male gametes, one male gamete moves towards egg cell and fuses with its nucleus to form a diploid cell called zygote and another male gamete fuses with two polar nuclei (secondary nuclei if they are already fused) to produce a triploid primary endosperm nucleus (PEN). OR (a) Structure of a sperm:
Plasma membrane Acrosome Head Nucleus containing chromosomal material

Neck Middle piece Mitochondria (energy source for swimming)

Tail

Functions: (i) Head: The acrosome contains hydrolytic enzymes which dissolves the egg membrane for fertilisation. (ii) Middle piece: It contains mitochondria that provide energy for the movement of the sperms. (iii) Tail: It helps in the motility of sperms that helps for the swimming of sperms in a liquid medium. (b) The sperm head becomes embedded in the Sertoli cells.

EXAMINATION PAPERS 2009 CBSE (All India) SETI

Time allowed: 3 hours Maximum marks: 70

General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. Section A contains 8 questions of one mark each, Section B is of 10 questions of two marks each, Section C has 9 questions of three marks each whereas Section D is of 3 questions of five marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.
SECTIONA

1. 2. 3. 4.

When and at what end does the tailing of hnRNA take place? Name the type of flower which favours cross pollination. Name the type of cells the AIDS virus enters into after getting in the human body. Name the unlabelled areas a and b of the pie chart representing the biodiversity of plants showing their proportionate number of species of major taxa.
Mosses Ferns

Lichens

5. According to HardyWeinbergs principle, the allele frequency of a population remains constant. How do you interpret the change of frequency of alleles in a population? 6. A boy of ten years had chicken-pox. He is not expected to have the same disease for the rest of his life. Mention how it is possible.1 7. Which one of the following is the bakers yeast used in fermentation? Saccharum barberi, Saccharomyces cerevisiae, Sonalika. 8. Why is bagging of the emasculated flowers essential during hybridisation experiments?

82
SECTIONB

Xam idea BiologyXII

9. What are recombinant proteins? How do bioreactors help in their production? 10. (a) Name the lymphoid organ in humans where all the blood cells are produced. (b) Where do the lymphocytes produced by the lymphoid organ mentioned above migrate and how do they affect immunity? 11. Draw a vertical section of a maize grain and label (i) pericarp, (ii) scutellum, (iii) coleoptile and (iv) radicle. OR Where are the Leydig cells present? What is their role in reproduction? 12. Explain the dual function of AUG codon. Give the sequence of bases it is transcribed from and its anticodon. 13. Explain the cause of algal bloom in a water body. How does it affect an ecosystem? 14. List the specific symptoms of typhoid. Name its causative agent. 15. Name the pioneer and the climax species in a water body. Mention the changes observed in the biomass and the biodiversity of the successive seral communities developing in the water body. 16. How is DNA isolated in purified form from a bacterial cell? 17. Explain metastasis. Why is it fatal? 18.
i p o z y a

mRNA

Transcription lac mRNA Translation Enzymes

Inducer

(a) Name the molecule X synthesised by i gene. How does this molecule get inactivated? (b) Which one of the structural genes codes for b-galactosidase? (c) When will the transcription of this gene stop?
SECTIONC

19. Fertilisation is essential for production of seed, but in some angiosperms, seeds develop without fertilisation. (i) Give an example of an angiosperm that produces seeds without fertilisation. Name the process. (ii) Explain the two ways by which seeds develop without fertilisation. 20. Explain any three measures which will control vehicular air pollution in Indian cities. 21. (a) Why do the symptoms of malaria not appear immediately after the entry of sporozoites into the human body when bitten by female Anopheles? Explain. (b) Give the scientific name of the malarial parasite that causes malignant malaria in humans.

Examination Papers 22.

b c

83

(a) What is this diagram representing? (b) Name the parts a, b and c. (c) In the eukaryotes, the DNA molecules are organised within the nulceus. How is the DNA molecule organised in a bacterial cell in absence of a nucleus? 23. Ornithologists observed decline in the bird population in an area near a lake after the setting of an industrial unit in the same area. Explain the cause responsible for the decline observed. 24. Recently a girl baby has been reported to suffer from haemophilia. How is it possible? Explain with the help of a cross. OR What are satellite DNA in a genome? Explain their role in DNA fingerprinting. 25.

(a) Write your observations on the variations seen in the Darwins finches shown above. (b) How did Darwin explain the existence of different varieties of finches on Galapagos Islands? 26. Name and explain the techniques used in the separation and isolation of DNA fragments to be used in recombinant DNA technology. 27. The following graph represents the organismic response to a certain environmental condition (e.g., temperature):
a b

Internal level

External level

84 (i) (ii) (iii) (iv)

Xam idea BiologyXII Which one of these, a or b, depicts conformers? What does the other line graph depict? How do these organisms differ from each other with reference to homeostasis? Mention the category to which humans belong.
SECTIOND

28. How did Hershey and Chase prove that DNA is the hereditary material? Expain their experiment with suitable diagrams. OR A particular garden pea plant produces only violet flowers. (a) Is it homozygous dominant for the trait or heterozygous? (b) How would you ensure its genotype? Explain with the help of crosses. 29. How does the pollen mother cell develop into a mature pollen grain? Illustrate the stages with labelled diagrams. OR Study the flow chart given below. Name the hormones involved at each stage and explain their functions. Hypothalamus Pituitary Ovary Pregnancy 30. How is a transgenic tobacco plant protected against Meloidegyne incognitia? Explain the procedure. OR (a) Name the source of Taq polymerase. Explain the advantage of its use in biotechnology. (b) Expand the name of the enzyme ADA. Why is this enzyme essential in the human body? Suggest a gene therapy for its deficiency.

Examination Papers

85

SETII
(Uncommon Questions to SetI) SECTIONA

1. Mention the strategy used to increase homozygosity in cattle for desired traits.
SECTIONB

9. MOET programme has helped in increasing the herd size of the desired variety of cattle. List the steps involved in conducting the programme. 11. Explain any two devices by which autogamy is prevented in flowering plants. OR Why do some women use Saheli pills? 18. Name the type of cells the AIDS virus first enters into after getting inside the human body. Explain the sequence of events that the virus undergoes within these cells to increase their progeny.
SECTIONC

19. Particulate and gaseous pollutants along with harmless gases are released from the thermal power plants. (i) Name any two harmless gases released. (ii) Name the most widely used device of removing particulate pollutants from the air. Explain how the device is used. 22. (a) Draw a schematic representation of the structure of a transcription unit and show the following in it: (i) Direction in which the transcription occurs (ii) Polarity of the two strands involved (iii) Template strand (iv) Terminator gene (b) Mention the function of promoter gene in transcription. OR (a) In human genome which one of the chromosomes has the most genes and which one has the fewest? (b) Scientists have identified about 1.4 million single nucleotide polymorphs in human genome. How is the information of their existence going to help the scientists? 27. Plasmid is a boon to biotechnology. Justify this statement quoting the production of human insulin as an example.
SECTIOND

30. (a) How did Griffith explain the transformation of R strain (non-virulent) bacteria into S strain (virulent)? (b) Explain how MacLeod, McCarty and Avery determined the biochemical nature of the molecule responsible for transforming R strain bacteria into S strain bacteria. OR (a) You are given tall pea plants with yellow seeds whose genotypes are unknown. How would you find the genotype of these plants? Explain with the help of cross.

86 (b) Identify a, b and c in the table given below: Pattern of inheritance 1. 2. 3. Codominance b Incomplete dominance

Xam idea BiologyXII

Monohybrid F1 phenotypic expression a The progeny resembled only one of the parents c

SETIII
(Uncommon Questions to SetI and II) SECTIONA

1. What type of virus causes AIDS? Name its genetic material. 3. Milk starts to coagulate when Lactic Acid Bacteria (LAB) is added to warm milk as a starter. Mention any other two benefits LAB provides. 7. Which of the following is a cyanobacterium that can fix atmospheric nitrogen? Azospirillum, Oscillatoria, Spirulina.
SECTIONB

9. Study the given portion of double stranded polynucleotide chain carefully. Identify a, b, c and the 5 end of the chain.
d P H | C | H H | C | H

OH

b a c e OH H | C | H P H | C | H

10. Name one plant and the addictive drug extracted from its latex. How does this drug affect the human body?
SECTIONC

19. (a) How does activated sludge get produced during sewage treatment? (b) Explain how this sludge is used in biogas production.

Examination Papers

87

25. Construct a pyramid of biomass starting with phytoplanktons. Label 3 trophic levels. Is the pyramid upright or inverted? Why? 27. Name the source and the types of cry genes isolated from it for incorporation into crops by biotechnologists. Explain how have these genes brought beneficial changes in the genetically modified crops.
SECTIOND

28. (a) When and how does placenta develop in human female? (b) How is the placenta connected to the embryo? (c) Placenta acts as an endocrine gland. Explain. OR (a) Trace the development of embryo after syngamy in a dicot plant. (b) Endosperm development precedes embryo development. Explain. (c) Draw a diagram of a mature dicot embryo and label cotyledons, plumule, radicle and hypocotyl in it.

88

Xam idea BiologyXII

SOLUTIONS
All India-2009 (Set I, II, III)

SETI
SECTIONA

1. Tailing of hnRNA take place during conversion of hnRNA into functional mRNA, it takes place at 3-end. 2. Chasmogamous flowers. 3. Macrophages or Helper T-cells. 4. a Fungi; b Angiosperms 5. Change of frequency of allele in a population will result in natural selection leading to the evolution. 6. When the boy encounters a pathogen for the first time, his body produces antibodies which develop memory of the first encounter to protect the body in future. 7. Saccharomyces cerevisiae. 8. Bagging of the emasculated flower is essential to prevent the entry of unwanted pollen (pollination) during hybridisation experiments.
SECTIONB

9. The proteins produced by genetically altered gene in a host are called recombinant proteins. Bioreactors are considered as vessels in which raw materials are biologically converted into specific products by microbes. It provides optimum growth conditions such as temperature, pH, substrate, vitamins, oxygen and salts. 10. (a) Bone marrow (b) The lymphocytes produced migrate to secondary lymphoid organs like spleen, lymph nodes, etc. They trap the microorganism thereby activating the lymphocytes present in the lymph nodes and produce an immune response affecting the immunity. 11.
Pericarp

Coleoptile Scutellum Radicle

V.S. of maize grain

OR

Examination Papers

89

The regions outside the seminiferous tubules called interstitial spaces contain interstitial cells or Leydig cells. These synthesise and secrete male sex hormone (androgens) testosterone. 12. The dual function of AUG codon: (a) It codes for amino acid methionine. (b) It is an initiation codon. The sequence of bases from which it is transcribed is TAC. Its anticodon is UAC. 13. Domestic sewage and industrial effluents contain nutrients like nitrogen and phosphorus which favour the excessive growth of planktonic (free-floating) algae called algal bloom. Its harmful effects cause: (i) sharp decline in dissolved oxygen content in the water. (ii) deterioration of water quality and causes mortality of aquatic life forms. 14. Specific symptoms of typhoid are: (i) Constant high fever (39 to 40C) (ii) Weakness (iii) Stomach pain (iv) Loss of appetite Its causative agent is Salmonella typhi. 15. Pioneer speciesPhytoplanktons Climax speciesForest or trees. Biomass will be gradually increased and phytoplanktons are replaced by free-floating angiosperms then by rooted hydrophytes followed by different seral communities thus, biodiversity increases. 16. DNA, a genetic material is isolated in purified form by treating the bacterial cells with the enzymes such as lysozyme to remove the cell wall. The RNA thus released can be removed by treating them with ribonuclease and enzyme proteases is added to remove proteins. Finally, chilled ethanol is added to precipitate the purified DNA. 17. The cancerous cells are sloughed from the tumours and reach distant sites through blood, and whenever they get lodged in the body, they start a new tumour there. This property called metastasis is the most feared property of malignant tumours. 18. (a) The molecule X is repressor. It gets inactivated when lactose (inducer) binds with the repressor molecule. (b) z gene codes for b-galactosidase. (c) Transcription of this gene stops when lactose is absent and thus repressor is free to bind with the operator.
SECTIONC

19. (i) In the members of family Asteraceae, seeds develop without fertilisation. This process is called apomixis. (ii) Two ways by which seeds develop without fertilisation: (a) In some species, the diploid (2n) egg cell is formed without reduction division and develops into embryo without fertilisation.

90

Xam idea BiologyXII (b) In many varieties of Citrus and Mango fruits some of the nucellar cells surrounding the embryo sac starts dividing, protrudes into the embryo sac and develops into embryos. 20. Three measures to control vehicular air-pollution in Indian cities are: (i) Use of CNG as fuel in the automobiles because it burns more efficiently and little of it is left unburnt. It is also cheaper. (ii) Use of unleaded petrol. (iii) Use of catalytic converter in the vehicles as it reduces emission of poisonous gases. 21. (a) Malarial parasite completes its asexual cycle in liver cells and then it attacks the red blood cells (RBCs) resulting in their rupture. The ruptured RBCs release toxic substance called haemozoin that is responsible for the symptoms of malaria like chill and high fever. Thus, no symptoms appear in the infected person between the period the parasite enters the body till RBCs release haemozoin. (b) Plasmodium falciparum 22. (a) Nucleosomes (b) a Histone octamer b DNA c H1 histone (c) In bacterial cell, DNA in nucleoid is organised in large loops held together by proteins. 23. DDT must have entered the trophic levels of food chain (biomagnification). It was accumulated in the birds as it can neither be metabolised nor be excreted. High concentration of DDT disturb calcium metabolism in birds which causes thinning of the eggshells and their premature breaking, ultimately causing decline in bird populations. 24. It is possible to have a haemophilic girl when a cross is made between a carrier woman and haemophilic man. Parents (Carrier woman) (Haemophilic man) Genotypes Gametes X XX h Xh X X
h

Xh

X hY Y Xh XhXh (Haemophilic girl) X hY (Haemophilic boy)

XX h (Carrier girl) XY (Normal boy)

OR

Examination Papers

91

A small stretch of DNA sequence repeats many a time, shows a high degree of polymorphism and form a bulk of DNA in a genome called as satellite DNA. DNA are very specific in each individual and vary in number from person to person but are inherited. Each individual inherits these repeats from his/her parents which are used as genetic markers in DNA fingerprinting. 25. (a) From the original seed-eating features, many other forms with altered beaks arose, enabling them to become insectivorous and vegetarian finches. (b) Darwin explained it as the process of evolution of different species in a given geographical area starting from a point and literally radiating to other areas of geography (habitats), called adaptive radiation. 26. Gel electrophoresis: The DNA fragments are negatively charged molecules and they can be separated by forcing them to move towards the anode under the influence of an electrical field through a medium or matrix. The DNA fragments separate according to size, i.e., smaller the size the farther it moves. The separated DNA can be seen only after staining the DNA by ethidium bromide (EtBr) followed by exposure to UV radiation. The separated bands of radiation are cut out and extracted from the gel piece by elution. 27. (i) a depicts conformers (ii) Response of the regulators. (iii) Conformers Aquatic animals and plants in which the osmotic concentration of body fluids c h a n g e s a c c o rd i n g t o t h e a mb i e n t conditions of water are called conformers. (iv) Regulators.
SECTIOND

Regulators Some organisms are able to maintain homeostasis by physiological means which ensures constant body temperature, constant osmotic concentration, etc.

28. Procedure: (i) Hershey and Chase grew some bacteriophage virus on a medium that contained radioactive phosphorus (32P) and some in another medium with radioactive sulphur (35S). (ii) Viruses grown in the presence of radioactive phosphorus (32P) contained radioactive DNA. (iii) Similar viruses grown in presence of radioactive sulphur (35S) contained radioactive protein. (iv) Both the radioactive virus types were allowed to infect E. coli separately. (v) Soon after infection, the bacterial cells were gently agitated in blender to remove viral coats from the bacteria. (vi) The culture was also centrifuged to separate the viral particle from the bacterial cell. Observations and Conclusions: (i) Only radioactive 32P was found to be associated with the bacterial cell, whereas radioactive 35S was only found in surrounding medium and not in the bacterial cell. (ii) This indicates that only DNA and not protein coat entered the bacterial cell.

92

Xam idea BiologyXII (iii) This proves that DNA is the genetic material which is passed from virus to bacteria and not protein. Note: Students either draw the labelled diagram or explain (both not necessary). OR (a) It could be homozygous dominant or heterozygous dominant. (b) By performing test cross, genotype can be determined. If parent homozygous If parent heterozygous Parents VV vv Vv vv (violet) (white) (violet) (white) Gametes V v V v v

Vv vv Violet white flowers flowers 50% : 50% 29. Pollen mother cells undergo meiosis to form a microspore tetrad (microspores arranged in a cluster of four cells) by the process called microsporogenesis. The microspores dissociate from each other and develop into pollen grains. The protoplast (pollen grain) then divides mitotically to form two unequal cells the bigger vegetative cell and smaller generative cell. F1 generation
Vegetative cell Asymmetric spindle Generative cell

Vv (All violet flowers)

Vacuoles Nucleus

OR Gonadotropin releasing hormone (GnRH) is a hypothalamic hormone and is secreted by the hypothalamus. It stimulates the anterior lobe of pituitary gland to secrete luteinising hormone (LH) and follicle stimulating hormone (FSH). They stimulate the growth of ovarian follicles and development of egg/oocyte within the follicle. After ovulation, remaining parts of the Graafian follicle transform as the corpus luteum. Corpus luteum secretes a large amount of progesterone which is essential for the maintenance of endometrium which is required for implantation of the embryo and other events of pregnancy. 30. Meloidegyne incognitia, a nematode, infects the roots of tobacco plants thereby causing reduction in yield. RNA interference (RNAi) process is used to develop transgenic tobacco plant that protects the plant from Meloidegyne incognitia. This method involves silencing of a specific mRNA due to complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). Using Agrobacterium vectors, nematode-specific genes were

Examination Papers

93

introduced into the host plant and produces both sense and anti-sense RNA in the host cells. These two RNAs being complementary to each other form dsRNA that initiated RNAi and thus, silenced the specific mRNA of the nematode. The parasite could not survive in a transgenic host because no protein is synthesised. OR (a) A bacterium, Thermus aquaticus is the source of Taq polymerase. It is a thermostable DNA polymerase that remains active even at high temperature and does not get denatured during denaturation of double stranded DNA in PCR. (b) ADAAdenosine deaminase. This enzyme is essential for immune system to function. ADA deficiency can be cured by gene therapy. Lymphocytes from the blood of the patients are extracted and cultured outside the body. A functional ADA cDNA (using a retroviral vector) is introduced into these lymphocytes and these lymphocytes are then returned to the patient's body. However, as these cells are not immortal, the patient requires periodic infusion of such genetically engineered lymphocytes.

94

Xam idea BiologyXII

SETII
SECTIONA

1. Inbreeding.
SECTIONB

9. The different steps involved in Multiple ovulation embryo transfer technology (MOET) are: (i) A cow is administered hormones, with FSH-like activity to induce follicular maturation and superovulation, i.e., 6-8 eggs are produced per cycle instead of one egg, which they normally yield per cycle. (ii) The superovulated animal is either mated with an elite bull or artificially inseminated. (iii) The fertilised egg at 8-32 cells stages are recovered and are transferred to surrogate mothers. 11. Autogamy is prevented and cross-pollination is favoured in flowering plants by following devices: (i) Dichogamy: Sometimes the stamens and stigma of a bisexual flower mature at different time to prevent autogamy. (ii) Self incompatibility being genetic mechanism prevents self-pollination. OR Saheli pills are used by women as oral contraceptive pills. It is a once a week pill having high contraceptive value and very few side-effects. 18. After the entrance of the virus into the body of the person, the virus enters into macrophages, where RNA genome of the virus replicates and forms viral DNA by the help of the enzyme reverse transcriptase. The viral DNA gets incorporated into the host cells DNA and directs the infected cells to produce viruses. The macrophages continue to produce virus that enters the helper T-lymphocytes. Thus, the number of helper T-lymphocytes progressively decreases in the body of the infected person.
SECTIONC

19. (i) Nitrogen and oxygen. (ii) Electrostatic precipitator. Electrostatic precipitator has electrode wires that are maintained at an electric current of several thousand volts, which produces a corona that releases electrons. These electrons attach to dust particles and give them a negative charge. Collecting plates are earthed that attract charged dust particles.

Examination Papers 22. (a)

Template strand Terminator 5 3

95

Schematic structure of a transcription unit

(i) Transcription occurs in 5 3 (b) Promotor gene has DNA sequence that provide binding site for RNA polymerase. OR (a) Chromosome 1 has most genes and the Y has the fewest gene. (b) The information regarding the occurrence of 1.4 million SNPs (single nucleotide polymorphism) revolutionise the processes of finding chromosomal locations for diseaseassociated sequences and tracing human ancestory (history). 27. Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria. In 1983, Eli Lilly an American company, first prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. These chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin (humulin).
SECTIOND

30. (a) Griffith introduced heat-killed S- strain bacteria Streptococcus in to a mouse along with live, R strain bacteria. The mouse died but the scientist recovered living S strain bacteria from the dead mouse. The conclusion Griffith arrived at after the experiment is that the R strain bacteria had somehow been transformed by the heat-killed S strain bacteria. Some transforming principle transferred from the heat-killed S strain, had enabled the R strain to synthesise a smooth polysaccharide coat and become virulent. This could only be possible if there was transfer of genetic material. (b) MacLeod, McCarty and Avery worked to determine the biochemical nature of the transforming principle in Griffiths experiment. They extracted purified DNA, RNA, proteins and other materials from heat-killed S bacteria nucleoid and mixed R bacteria with these different materials, and only those mixed with DNA were transformed into S bacteria. This experiment strongly implied that DNA is the transforming factor and not proteins or other materials and by this it was demonstrated what is known to us as the transforming principle - that genes are made of DNA and DNA is the hereditary material.

96 OR (a) Test cross will be performed to know the genotype of these plants. (i) Parents Tall plants Dwarf plants yellow seeds green seeds Genotypes TTYY ttyy Gametes F1 generation (ii) Parents Genotypes Tall plants yellow seeds TtYy TY ty

Xam idea BiologyXII

TtYy Tall plants, yellow seeds. Dwarf plants green seeds ttyy

Gametes

TY

Ty

tY

ty

ty

TtYy Ttyy ttYy ttyy Tall plants, Tall plants, Dwarf plants Dwarf plants yellow seeds green seeds yellow seeds green seeds The phenotypic and genotypic ratios are 1: 1: 1: 1. (b) aBoth the forms of a trait are equally expressed in F1 generation. bDominance. cPhenotypic expression of F 1 generation is somewhat intermediate between the two parental forms of a trait.

F1 generation

Examination Papers

97

SETIII
SECTIONA 1. Retrovirus. RNA is its genetic material. 3. Two benefits provided by LAB: (i) increases nutritional quality of curd by increasing vitamin B12 content. (ii) checks the growth of disease-causing organisms in the gut. 7. Oscillatoria. SECTIONB 9. aHydrogen bonds. bPurines, i.e., adenine or thymine. cPentose sugar (deoxyribose sugar). d5 end. 10. Papaver somniferum (poppy plant). Additive drug extracted from its latex is opioids, morphine, heroin, etc. It is a depressant and slows down body functions. SECTIONC 19. (a) Formation of activated sludge: Once the BOD of sewage or waste water is reduced significantly, the effluent is passed into a settling tank where the bacterial flocs undergo sedimentation and the sediment is thus called activated sludge. (b) A small part of the activated sludge is pumped back into the aeration tank to serve as the inoculum. The remaining major part of the activated sludge is pumped into large tanks called anaerobic sludge digesters. The anaerobic bacteria digest the bacteria and fungi in the sludge and produces mixture of gases like methane, hydrogen sulphide and CO 2 which constitute biogas. 25.
T3 T2 T1 Pyramid of biomass Fishes Zooplanktons Phytoplanktons

The pyramid is inverted because the biomass of fishes is much more than that of the phytoplanktons. 27. Source of cry geneBacillus thuringiensis. The following type of cry genes are isolated from it: cryIAc, cryIIAb, cryIAb. The introduction of cry gene acts as biopesticide. The cry gene produce crystals of toxic insecticidal protein. The activated toxin causes death of the insect. SECTIOND 28. (a) Placenta develops after implantation of embryo in human female in the uterus. After implantation, finger-like projections called chorionic villi are surrounded by the uterine tissue and maternal blood. The chorionic villi and uterine tissue become interdigitated with each other and together form a structural and functional unit between developing embryo and maternal body called placenta. (b) An umbilical cord connects placenta with the embryo. (c) The placenta also acts as an endocrine gland and secretes the following hormones: (i) Human chorionic gonadotropin (hCG),

98

Xam idea BiologyXII (ii) Human placental lactogen (hPL), (iii) Progestogens, and (iv) Estrogens The hormone hCG and hPL and relaxin are only produced during pregnancy that maintains the pregnancy. The increased production of these hormones during pregnancy is essential for supporting the fetal growth and metabolic changes in the mother. OR (a)
Degenerating synergids Zygote (2n) Primary endosperm cell (PEC) Primary endosperm nucleus (3n) (PEN) Suspensor Radicle Zygote Cotyledon Degenerating antipodal cells (a) Heart-shaped embryo Plumule Mature embryo Globular embryo (b)

(a) Fer tilised embryo sac showing zygote and Primary Endosperm Nucleus (PEN); (b) Stages in embryo development in a dicot [shown in reduced size as compared to (a)]

(b) The endosperm development precedes embryo development. The developing embryo requires nutrition for its development that is provided by the endosperm. (c)
Plumule

Cotyledons Hypocotyl

Radicle Root cap

A typical dicot embryo

EXAMINATION PAPERS 2009 CBSE (Foreign) SETI

Time allowed: 3 hours Maximum marks: 70

General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. Section A contains 8 questions of one mark each, Section B is of 10 questions of two marks each, Section C has 9 questions of three marks each whereas Section D is of 3 questions of five marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.
SECTIONA

1. At which ends do capping and tailing of hnRNA occur respectively? 2. The meiocyte of rice has 24 chromosomes. How many chromosomes are present in its endosperm? 3. BOD of two samples of water A and B were 120 mg/L and 400 mg/L respectively. Which sample is more polluted? 4.
Mammals

Birds a Reptiles b

5. 6. 7. 8.

Name the unlabelled areas a and b of the pie chart representing biodiversity of vertebrates showing the proportionate number of species of major taxa. Are the wing of a bird and the forelimb of a horse homologous or analogous? Name the type of evolution that explains the development of such structures. Name the type of interaction seen between whale and the barnacles growing on its back. Which one of the following is used in apiculture: Hilsa, Apis indica, Sonalika The following statements (i), (ii) and (iii) seem to describe the water-pollinated submerged plants. Which one of these statements is incorrect? (i) The flowers do not produce nectar. (ii) The pollen grains have mucilaginous covering. (iii) The brightly coloured female flowers have long stalk to reach the surface.

100
SECTIONB

Xam idea BiologyXII

9. Name the source organism from which Ti plasmid is isolated. Explain the use of this plasmid in biotechnology. 10. (a) Explain the property that prevents normal cells from becoming cancerous. (b) All normal cells have inherent characteristic of becoming cancerous. Explain. 11. In the T.S. of a mature anther given below, identify a and b and mention their function.

a b

12. Name the category of codons UGA belongs to. Mention another codon of the same category. Explain their role in protein synthesis. 13. How do automobiles fitted with catalytic converters reduce air pollution? Suggest the best fuel for such vehicles. 14. List the specific symptoms of pneumonia. Name the causative organism. OR How does spleen act as a lymphoid organ? Explain. 15. How does the Mediterranean orchid Ophrys ensure its pollination by bees? 16. Highlight any four advantages of genetically modified organisms (GMOs). 17. What is colostrum? Why is it important to be given to the newborn infants? 18.
i p o z y a

(a) Name the molecule M that binds with the operator. (b) Mention the consequences of such binding. (c) What will prevent the binding of the molecule M with the operator gene? Mention the event that follows.

Examination Papers
SECTIONC

101

19.

(a) Identify the figure. (b) Name the initial cell from which this structure has developed. (c) Draw the next mature stage and label the parts. 20. A particular species of wild cat is endangered. In order to save them from extinction, which is a desirable approach in situ or ex situ? Justify your answer and explain the difference between the two approaches. 21. (a) What is EcoRI? What does R represent in this? (b) Give the palindromic nucleotide sequence recognised by it. (c) Explain its action. 22.

SER

C G AG CUGAGUU a AA a'

(a) Identify the polarity from a to a' in the above diagram and mention how many more amino acids are expected to be added to this polypeptide chain. (b) Mention the DNA sequence coding for serine and the anticodon of tRNA for the same amino acid. (c) Why are some untranslated sequence of bases seen in mRNA coding for a polypeptide? Where exactly are they present on mRNA? 23. Why is predation required in a community of different organisms? 24. (a) Name the virus that causes AIDS in humans. (b) Explain the sequence of events that follows when this virus attacks to cause immune deficiency in humans.

102

Xam idea BiologyXII 25. In one family, each of the four children has a different blood group. Their mother is group A and the father is group B. Explain this pattern of inheritance with the help of a cross along with the genotypes. OR Who proposed chromosomal theory of inheritance? Point out any two similarities in the behaviour of chromosomes and genes. 26.

(a)

(b)

What do these pictures a and b illustrate with reference to evolution? Explain. 27. Draw and complete the following model of carbon cycle filling a, b, c, d, e and f.
CO2 Atmosphere Food a b c d

Plants

Animals

Calcareous sediments

Examination Papers
SECTIOND

103

28. (a) Explain the observations of Meselson and Stahl when (i) they cultured E. coli in a medium containing 15 NH 4 Cl for a few generations and centrifuged the content. (ii) they transferred one such bacterium to the normal medium of NH 4 Cl. (b) What does the above experiment prove? (c) Which is the first genetic material identified? OR Inheritance pattern of flower colour in garden pea plant and snapdragon differs. Why is the difference observed? Explain showing the crosses. 29. (a) Draw a labelled diagram of a mature embryo sac of an angiosperm. (b) Why does a pollen grain possess two male gametes? Explain. OR Study the flow chart given below. Name the hormones involved at each stage and explain their role. Ovulation Pregnancy Placenta Foetal growth Lactation Parturition 30. (a) Why are engineered vectors preferred by biotechnologists for transferring the desired genes into another organism? (b) Explain how do ori, selectable markers and cloning sites facilitate cloning into a vector. OR (a) How is a transgenic tobacco plant protected against Meloidegyne incognitia? (b) Explain the procedure of making such plants.

104

Xam idea BiologyXII

SETII
(Uncommon Questions to Set I) SECTIONA

2. Why is a coconut plant referred to as monoecious? 5. Are flippers of penguin and dolphin homologous or analogous? What type of evolution has brought such a similarity in them? 8. Which of the following is the semi-dwarf wheat that is high yielding and disease resistant? Pusa Shubra, Kalyan Sona, Ratna.
SECTIONB

13. Differentiate between a template strand and a coding strand of DNA. 15. (a) How is Cuscuta adapted to be a parasitic plant? (b) Why do cattle avoid browsing on Calotropis plants? Explain.
SECTIONC

22. How is innate immunity different from the immunity that you acquire through vaccines? Describe any two ways by which innate immunity can be accomplished. 25. Name the source of gonadotropins in human females. Explain the changes brought about in the ovary by these hormones during menstrual cycle. 27. Why are (i) alien species invasion and (ii) loss of habitat and fragmentation considered to be the major cause of loss of biodiversity? Explain with the help of one example each.
SECTIOND

28. (a) Draw a labelled diagram of L.S. of a flower to show the growth of pollen tube reaching egg apparatus. (b) Pistil of a flower does not accept pollen from any plant other than from its own kind. How does it happen? Explain. OR (a) Given below is the T.S. of human ovary. Identify the following in the diagram:
e b a

c d

(i) Corpus luteum (ii) Secondary oocyte (iii) Antrum (iv) Primary follicle (v) Primary oocyte (b) Explain the changes the primary oocyte undergoes while in different follicular stages before ovulation.

Examination Papers

105

SETIII
(Uncommon Questions to Set I and II) SECTIONA

1. Name the type of pollination as a result of which genetically different types of pollen grains of the same species land on the stigma. 3. Are the thorns of Bougainvillea and tendrils of Cucurbita homologous or analogous? What type of evolution has brought such a similarity in them? 7. Which of the the following produces single cell proteins? Sonalika, Spirulina, Saccharomyces.
SECTIONB

10. How is Darwins concept of evolution different from that of de Vries? 15. Identify the curves a and b shown in the graph given below. List the conditions responsible for growth patterns a and b.
k
Population density (N)

Time (t) Population growth curve

SECTIONC

19. How are assisted reproductive technologies helpful to humans? How are ZIFT and GIFT different from intra uterine transfers? Explain. 25. (a) Baculoviruses are excellent candidates for integrated pest management in an ecologically sensitive area. Explain giving two reasons. (b) What is organic farming? Why is it suggested to switch over to organic farming? 27. How did Eli Lilly Company go about preparing the human insulin? How is the insulin thus produced different from that produced by the functional human insulin gene?
SECTIOND

29. Explain the steps of DNA fingerprinting that will help in processing of the two blood samples A and B picked up from the crime scene. OR A particular garden pea plant produced only violet flowers. It may or may not be homozygous dominant for the trait. How would you ensure its genotype? Explain with crosses.

106

Xam idea BiologyXII

SOLUTIONS
SETI SECTIONA

Capping occurs at 5-end and tailing occurs at 3-end. 36 chromosomes Sample B is more polluted than sample A. aFishes; bAmphibians. Homologous. Divergent evolution. 6. Commensalism 7. Apis indica 8. Statement-(iii). 1. 2. 3. 4. 5.
SECTIONB

9. Ti plasmid is isolated from Agrobacterium tumifaciens. Ti plasmid of Agrobacterium tumifaciens has been modified into a cloning vector which is no more pathogenic to plants but still able to use the mechanisms to deliver genes of interest into plants. 10. (a) By the contact inhibition, normal cells do not change into cancerous cells. (b) Several genes called cellular oncogenes (c-onc) or proto oncogenes in normal cells which, when activated under certain conditions, could lead to oncogenic transformation of the cells leading to cancer. 11. aSporogeneous tissue: It forms pollen grains. bTapetum: It provides nourishment to the developing pollen grains. 12. UGA is a stop or termination codon. UAA, UAG are the other stop codons of the category. They prevent the elongation of the polypeptide chain by terminating translation. 13. Catalytic converters have expensive metals like platinumpalladium and rhodium as catalysts. As exhaust emission passes through catalytic converter, unburnt hydrocarbons are converted into carbon dioxide and water, and carbon monoxide and nitric oxide are changed to carbon dioxide and nitrogen gas. Unleaded petrol is the best fuel. 14. Specific symptoms of pneumonia are: (i) fever, (ii) cough, (iii) headache, (iv) chills. It is caused by Streptococcus pneumoniae and Haemophilus influenzae. OR The spleen is a large bean-shaped organ. It mainly contains lymphocytes and phagocytes. It acts as a filter of the blood by trapping blood-borne microorganisms. Spleen also has a large reservoir of erythrocytes. 15. Pollination in Ophrys: The petals of the Ophrys resembles the female of a bee species in size, colour and odour, etc. Male bee mistakes the Ophrys for female bee and tries to copulate. Few pollen grains adhered with the body of the male bee fall over stigma of the flower thereby leading to pollination.

Examination Papers

107

16. Advantages of GMOs: (i) tolerant against abiotic stresses (cold, drought, salt, heat). (ii) reduces reliance on chemical pesticides. (iii) reduces post-harvest losses. (iv) increase efficiency of mineral usage by plants. 17. The milk that comes out of the mammary glands during initial days of lactation is called colostrum. It contains several antibodies absolutely essential for developing resistance for the new-born babies. 18. (a) M is the repressor. (b) When repressor binds with the operator, transcription stops. (c) An inducer prevents the binding of repressor to operator and thus transcription starts.
SECTIONC

19. (a) It is globular embryo of a dicot plant. (b) Zygote (c)

Suspensor

Heart-shaped embryo

20. Ex situ is a desirable approach to protect the wild cat. The organism is protected outside their natural habitat where special care is taken to protect them. By using cryopreservation techniques, gametes of threatened species can be preserved under very low temperature. In situ Conservation (i) Ex situ Conservation

It is conservation and protection of It is conservation of selected rare plants and biodiversity in its natural habitat. animals in places outside their natural habitat. Ecologically unique and biodiversity-rich Zoological parks, botanical gardens and regions are legally protected as biosphere wildlife safari parks serve this purpose. reserves, national parks and sanctuaries.

(ii)

21. (a) EcoRI is a restriction endonuclease, obtained from an E. coli bacterium. R represents the name of the strain. (b) 5 GAATTC 3 3 CTTAAG 5 (c) It cuts the DNA between bases G and A on both the strands only when the sequence GAATTC is present in the DNA.

108

Xam idea BiologyXII 22. (a) Polarity from a to a is 5 3 No more amino acid will be added to this polypeptide chain. (b) DNA sequence for serine is AGU; anticodon is UCA. (c) The untranslated sequence of bases are required for efficient translation process. They are present before the start codon at the 5-end and after the stop codon at 3-end. 23. Reasons: (i) It keeps the prey populations under control. (ii) It helps in maintaining species diversity in a community by reducing the intensity of competition among competing prey species. (iii) It acts as a conduit for energy transfer across trophic levels. (iv) Biological control methods adopted in agricultural pest control are based on the ability of the predator to regulate prey population. 24. (a) Human Immunodeficiency Virus. (b) After the entry of the virus into the body of the person, the virus enters into macrophages, where RNA genome of the virus replicates and form viral DNA by the help of the enzyme reverse transcriptase. The viral DNA gets incorporated into the host cells DNA and directs the infected cells to produce viruses. The macrophages continue to produce virus that enters the helper T-lymphocytes. Thus, the number of helper T-lymphocytes progressively decreases in the body of the infected person. 25. A cross is carried out between heterozygous father (for blood group B) and heterozygous mother (of blood group A) to get four children with different blood groups. Parents Genotypes Father (B group) IB i Gametes IB i IA Mother (A group) IA i I

F1 generation

IB IA AB blood group

IB i B blood group

IA i A blood group

ii O blood group

All the four blood groups are controlled by three allelic genes I A , I B , i and thus it shows phenomena of multiple allelism. Both I A and I B is dominant over i. However, when both are present together, both are dominant and show the phenomena of co-dominance forming the blood group AB. Six genotypes are possible with combination of these three alleles. OR It was proposed by Sutton and Boveri. Similarities: (i) Both genes and chromosomes occur in pairs in a diploid cell (2n). (ii) Both of them separate out during gametogenesis to enter into different gametes.

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109

26. aA white-winged moth and dark-winged moth on a tree trunk in an unpolluted area. bA white-winged moth and a dark-winged moth on a tree trunk in a polluted area. Industrial melanism: In England, it was observed before industrialisation that white-winged moth were more than dark-winged moth. But the situation became reversed after industrialisation. In pre-industrialisation, the tree trunk was covered by white lichen and on white background dark-coloured moth could be picked up. The situation changed after industrialisation, the tree was covered by industrial smoke and soots and became dark on which white-winged moth could easily be picked up by predators. It was found that industrial melanism supported evolution by natural selection. 27. aPhotosynthesis bRespiration cRespiration dCombustion of fossil fuels eAquatic food chain fCoal, oil.
SECTIOND

28.(a) (i) Meselson and Stahl observed that in the E. coli bacterium the DNA becomes completely labelled with N 15 medium by centrifugation for few generations. (ii) After two generations, density changed and showed equal amount of light DNA (N14) and dark hybrid DNA (N 14 N 15 ). (b) They concluded that DNA replicates semi-conservatively.
Generation I
15

Generation II
14

N-DNA

14

N-DNA

N-DNA N-DNA N-DNA N-DNA

15

N-DNA 20 min 40 min

15

14

Gravitational force

14

15

N15 N Heavy

14

N15 N Hybrid

14

N14 N

14

N15 N Hybrid

Light

Meselson and Stahls Experiment (Separation of DNA by centrifugation)

(c) Ribonucleic acid (RNA) was the first genetic material. OR Inheritance pattern of flower colour in garden pea shows principle of dominance whereas in snapdragon shows incomplete dominance. Inheritance of flower colour in garden pea: Parents Purple flower White flower Genotypes PP pp Gametes P p

F 1 generation

Pp

(purple flowers)

110 On selfing P p Pp P PP (Purple flower) Pp (Purple flower) Phenotypic ratio 3: 1 Genotypic ratio 1: 2: 1. Inheritance of flower colour in snapdragon Parents Red flower Genotypes RR Gametes R Pp p Pp (Purple flower) pp (White flower)

Xam idea BiologyXII

White flower rr r

F 1 generation On selfing

Rr R RR R r (Red flower) Rr (Pink flower)

Rr (pink flowers) Rr r Rr (Pink flower) rr (White flower)

Phenotypic ratio 1: 2: 1 Genotypic ratio 1: 2: 1. 29. (a) A diagrammatic representation of the mature embryo sac.
Chalazal end Antipodals

Polar nuclei Central cell Egg Synergids

Micropylar end

Filiform apparatus

(b) Pollen grains possess two male gametes as it is required for double fertilisation in most angiosperms. Out of two male gametes, one male gamete moves towards egg cell and fuses with its nucleus to form a diploid cell called zygote and another male gamete fuses with two polar nuclei (secondary nuclei if they are already fused) to produce a triploid primary endosperm nucleus (PEN).

Examination Papers

111

l l l

OR Rapid release of luteinising hormone rupture Graafian follicle and release ovum (ovulation). Corpus luteum secretes large amount of progesterone hormone that is essential for maintenance of the endometrium required for implantation of blastocyst leading to pregnancy. Placenta produces several hormones like human chorionic gonadotropin (hCG), human placental lactogen (hPL). Relaxin is also produced during later phase of pregnancy. Level of other hormones like estrogens, progestogens, cortisol, prolactin and thyroxine also increases which is essential for supporting fetal growth, metabolic changes in mother and maintenance of pregnancy.

Parturition signals originate from the fully developed foetus and the placenta induce mild uterine contractions which triggers release of oxytocin from pituitary. Oxytocin acts on the uterine muscle causing stronger uterine contractions. 30. (a) Engineered vectors are preferred by biotechnologists because they help in easy linking of foreign DNA and selection of recombinants from non-recombinants. (b) Ori: This is a sequence from where replication starts and any piece of DNA when linked to this sequence can replicate within the host cells. It also controls copy number of linked DNA. So, in order to get many copies of the target DNA it should be cloned in a vector whose origin support high copy number. Selectable marker: It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. Cloning sites: To link the alien DNA, the vectors require very few recognition sites for the restriction enzymes.
l

OR (a) Meloidegyne incognitia, a nematode infects the roots of tobacco plants thereby causing reduction in yield. RNA interference (RNAi) process is used to develop transgenic tobacco plant that protects the plant from Meloidegyne incognitia. This method involves silencing of a specific mRNA due to complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). (b) Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant and produces both sense and anti-sense RNA in the host cells. These two RNAs being complementary to each other form dsRNA that initiated RNAi and thus, silenced the specific mRNA of the nematode. The parasite could not survive in a transgenic host because no protein is synthesised.

SETII
SECTIONA

2. A coconut plant has both male and female reproductive structures thus it is known as monoecious. 5. Yes, they are analogous. Convergent evolution. 8. Kalyan sona.

112
SECTIONB

Xam idea BiologyXII

13. S. No. (i) Template Strand Coding Strand The DNA strand that has the polarity The strand which does not code anything 3 - 5 act as a template for transcription and has polarity 5 -3 is called coding and known as template strand. strand. Nucleotide sequence is complementary to The nucleotide sequence is the same to the one present in mRNA the one present in mRNA except for the presence of thymine instead of uracil.

(ii)

15. (a) Cuscuta has lost its chlorophyll and leaves during evolution and thus it derives its nutrition from host plant thus it is a parasitic plant. (b) Cattle avoid browsing on Calotropis plants because it produces poisonous cardiac glycosides.
SECTIONC

22. S. No. (i) Innate Immunity Acquired Immunity It is a non-specific type of defence present It is pathogen specific which is not present from the birth in an individual and is from the birth and develops through inherited from the parents. vaccination. It does not cause side-effects. I t c a u s e c er t a i n re a c t i o n a g a i n s t vaccination.

(ii)

Innate immunity can be accomplished by the following barriers: (i) Physiological barriers: Tears in eyes, acid in the stomach, and saliva in mouth, etc. prevent microbial growth. (ii) Cytokine barriers: Interferons produced by virus-infected cells protect non-infected cells from further viral infection. 25. Gonadotropins (LH and FSH) are secreted by the anterior lobe of pituitary gland. l Gonadotropins (LH and FSH) increases gradually during the follicular phase (proliferative phase) of menstrual cycle and stimulates follicular development as well as secretion of estrogen by the growing follicles. l LH and FSH attain a peak level in the middle of the cycle (about 14th day) and rapid secretion of LH induces rupture of Graafian follicle followed by ovulation (release of ovum). l LH stimulates transformation of Graafian follicle into corpus luteum. 27. (i) The alien species become invasive and cause extinction or decline of indigenous species, e.g., the Nile perch introduced into Lake Victoria in east Africa led to the extinction of more than 200 species of cichlid fish in the lake. (ii) Loss of habitat and fragmentation drive animals and plants to extinction. E.g., when the Amazon forest is cut and cleared for cultivating soya beans or for conversion of grasslands for raising beef cattle, many species are affected.

Examination Papers
SECTIOND

113

28. (a) L.S. of pistil showing path of pollen tube growth

Pollen tube

Antipodal Polar nuclei Egg cell Synergid

(b) The pistil has the ability to recognise pollen, whether it is of right type (compatible) or of the wrong type (incompatible). It is mediated by chemical components of the pollen interacting with those of the pistil. (c) Syngamy is the fusion of male gamete with female gamete resulting in the formation of a diploid cell called zygote. OR (a) (i) dCorpus luteum (ii) cSecondary oocyte (iii) bAntrum (iv) ePrimary follicle (v) aPrimary oocyte (b) Changes in the primary oocyte:
l l l l l l

Each primary oocyte gets surrounded by a single layer of granulosa cells, known as primary follicles. Primary follicles get surrounded by more layers of granulosa cells and a new theca called secondary follicles. A secondary follicle transforms into tertiary follicle characterised by a fluid filled space, the antrum. Primary oocyte undergoes first meiotic division and produces two unequal haploid cells: a large haploid secondary oocyte and a tiny first polar body. The tertiary follicle further changes into the mature Graafian follicle. The secondary oocyte forms a new membrane called zona pellucida around it.

114

Xam idea BiologyXII

SETIII
SECTIONA

1. Xenogamy. 3. They are homologous organ. Divergent evolution has brought such a similarity in them. 7. Spirulina.
SECTIONB

10. S.No. (i) (ii) Darwins concept of evolution Continuous variations among individuals of a species is the basis of evolution. Darwinian variations are gradual. de Vries concept of evolution Mutations are the basis of evolution. de Vries mutation appear all of a sudden.

15. (a) Exponential growth curve: When the resources (food + space) are not limited, this type of growth curve appears. (b) Logistic growth curve: When the resources become limited at certain point of time, this type of growth curve appears.
SECTIONC

19. The infertile couples could be assisted to have children through certain special techniques known as assisted reproductive technologies (ART). ZIFT: The zygote or early embryo with upto 8 blastomeres is transferred into the fallopian tube called zygote intra fallopian transfer (ZIFT). GIFT: It is the transfer of an ovum collected from a donor into the fallopian tube of another female who cannot produce one but can provide suitable environment for fertilisation and further development of the embryo. Intra uterine transfer refers to the introduction of embryo with more than 8 blastomeres into the uterus of a female to complete its further development. 25. (a) Reasons: (i) Baculoviruses are biological control agents which are excellent candidates for species specific, narrow-spectrum insecticidal applications. (ii) They have no negative impacts on plants, mammals or even on non-target insects. (b) The use of biofertilisers and biopesticides to improve the crop yields as there are problems associated with over use of chemical fertilisers is known as organic farming. It is advised due to following reasons: (i) Use of excess chemical fertilisers make the soil unsuitable for cultivation by causing the pollution. (ii) Natural resources get depleted due to manufacturing of chemical fertilisers.

Examination Papers

115

27. Eli Lilly company of America (1983) prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains A and B. The extracted chains A and B were combined by creating disulfide bridges to produce functional insulin. The insulin (proinsulin) manufactured by the functional human insulin gene contains polypeptide chain A, B and an extra stretch called the C peptide which is removed during maturation.
SECTIOND

30. DNA Fingerprinting: This technique was discovered by Alec Jaffery in 1985. The technique involves following steps: (i) Isolation and extraction of DNA from the cell by centrifugation. (ii) By the help of enzyme restriction endonuclease DNA molecules are digested. The fragment also contains VNTRs. (iii) The small DNA fragments are separated through gel electrophoresis set-up that contain agarose polymer gel. (iv) The separated DNA fragments are transferred from electrophoresis plate to synthetic membranes like nitrocellulose or nylon membrane sheet called southern blotting. (v) The DNA probes are added which target a specific nucleotides sequence which is complementary to them and this process is called hybridisation (Southern blotting). (vi) The nylon membrane is exposed to an X-ray film and dark orange coloured bands developed at sites where probes have bound to the DNA fragments. This is known as autoradiography. On comparing the DNA prints of blood samples A and B, it can be confirmed that the blood sample picked up from the crime scene belongs to the same individual or to two different individuals. OR A test cross is required to find out the genotype of both the plant. (A) Garden pea (Homozygous red) Genotypes PP pp Gametes P p

F1 generation

Pp

(Purple flowers)

116

Xam idea BiologyXII If the F1 generation produces all purple flower the parent would be homozygous dominants, i.e., pp. (Heterozygous red) Genotypes Pp pp Gametes P p p

F1 generation

Pp

pp

Purple flower White flower If the F1 generation produces purple and white flowers in 1:1 ratio, the parent would be heterozygous, i.e., Pp.

EXAMINATION PAPERS 2010

CBSE (DELHI) SETI Time allowed: 3 hours General Instructions: (i) All questions are compulsory. (ii) This question paper consists of four sections A, B, C and D. Section A contains 8 questions of one mark each, Section B is of 10 questions of two marks each, Section C is of 9 questions of three marks each and Section D is of 3 questions of five marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled. SECTIONA 1. A bilobed, dithecous anther has 100 microspore mother cells per microsporangium. How many male gametophytes this anther can produce? 2. Mention two functions of the codon AUG. 3. Name the scientist who disproved spontaneous generation theory. 4. What is it that prevents a child to suffer from a disease he/she is vaccinated against? Give one reason. 5. Why is the enzyme cellulase used for isolating genetic material from plant cells but not for animal cells? 6. Name a molecular diagnostic technique to detect the presence of a pathogen in its early stage of infection. 7. If 8 individuals in a laboratory population of 80 fruit flies died in a week, then what would be the death rate of population for the said period? 8. Mention one positive and one negative application of amniocentesis. SECTIONB 9. A moss plant produces a large number of antherozoids but relatively only a few egg cells. Why? 10. Mention the reasons for difference in ploidy of zygote and primary endosperm nucleus in an angiosperm. 11. How does an electrostatic precipitator work to remove particulate pollutants released from the thermal power plants? 12. Name the type of food chains responsible for the flow of larger fraction of energy in an aquatic and a terrestrial ecosystem, respectively. Mention one difference between the two food chains. 13. How does a test-cross help in identifying the genotype of the organism? Explain. Maximum marks: 70

118

Xam idea BiologyXII

14. Name the host and the site where the following occur in the life-cycle of a malarial parasite: (a) Formation of gametocytes (b) Fusion of gametocytes 15. Honey collection improves when beehives are kept in crop-fields during the flowering season. Explain. OR How does addition of a small amount of curd to fresh milk help in the formation of curd? Mention a nutritional quality that gets added to the curd. 16. Why is the introduction of genetically engineered lymphocytes into an ADA deficiency patient not a permanent cure? Suggest a possible permanent cure. 17. How does the floral pattern of Mediterranean orchid Ophrys guarantee cross pollination? 18. In the biosphere immense biological diversity exists at all levels of biological organisation. Explain any two levels of biodiversity. SECTIONC 19. Draw a longitudinal section of a post-pollinated pistil showing entry of pollen tube into a mature embryo-sac. Label filiform apparatus, chalazal end, Hilum, antipodals, male gametes and secondary nucleus. OR Draw a labelled sectional view of seminiferous tubule of a human male. 20. During his studies on genes in Drosophila that were sex-linked. T.H. Morgan found F2 population phenotypic ratios deviated from expected 9 : 3 : 3 : 1. Explain the conclusion he arrived at. 21. Describe the initiation process of transcription in bacteria. 22. Explain convergent and divergent evolution with the help of one example of each. 23. Name the type of human cell HIV attacks on its entry into the body. Explain the events that occur in the cell which further lead to cause immunodeficiency syndrome. 24. Explain the efforts which must be put in to improve health, hygiene and milk yield of cattle in a dairy farm. 25. Identify a, b, c, d, e and f in the table given below: Organism 1. 2. 3. Monascus perpureus (yeast) c e Bioactive Molecule a d Cyclosporin A Use b antibiotic f

26. EcoRI is used to cut a segment of foreign DNA and that of a vector DNA to form a recombinant DNA. Show with the help of schematic diagrams. (i) The set of palindronic nucleotide sequence of base pairs the EcoRI will recognise in both the DNA segments. Mark the site at which EcoRI will act and cut both the segments.

Examination Papers

119

(ii) Sticky ends formed on both the segments where the two DNA segments will join later to form a recombinant DNA. 27. How does RNA interference help in developing resistance in tobacco plant against nematode infection? SECTIOND 28. (i) How does a Chromosomal disorder differ from a Mendelian disorder? (ii) Name any two chromosomal aberration associated disorders. (iii) List the characteristics of the disorders mentioned above that help in their diagnosis. OR Fitness is the end result of the ability to adapt and get selected by nature. Explain with suitable example. 29. When and where are primary oocytes formed in a human female? Trace the development of these oocytes till ovulation (in menstrual cycle). How do gonadotropins influence this developmental process? OR (i) Explain the events taking place at the time of fertilization of an ovum in a human female. (ii) Trace the development of the zygote upto its implantation in the uterus. (iii) Name and draw a labelled sectional view of the embryonic stage that gets implanted. 30. Draw and explain a logistic curve for a population of density (N) at time (t) whose intrinsic rate of natural increase is (r) and carrying capacity is (k). OR Describe the process of decomposition of detritus under the following heads: Fragmentation; leaching; catabolism; humification and mineralisation.

120

Xam idea BiologyXII

SETII
(Uncommon Questions to Set I) SECTIONA 6. An anther with malfunctioning tepetum often fails to produce viable male gametophytes. Give one reason. 8. In a pond there were 20 Hydrilla plants. Through reproduction 10 new Hydrilla plants were added in a year. Calculate the birth rate of the population. SECTIONB 9. Biodiversity must be conserved as it plays an important role in many ecosystem services that nature provides. Explain any two services of the ecosystem. 14. How many haploid cells are present in a mature female gametophyte of a flowering plant? Name them. 15. When a tall pea plant was selfed, it produced one-fourth of its progeny as dwarf. Explain with the help of a cross. 16. Chlorofluorocarbons (CFCs) are widely used as refrigerants. Then why is it suggested to reduce its emission as far as possible? Explain. SECTIONC 22.
N S

'a'

S S S S S S S S C 'b'

S S S S

Identify a, b and c in the schematic diagram of an antibody given above answer the questions. (i) Write the chemical nature of an antibody.

Examination Papers (ii) Name the cells that produce antibodies in humans. (iii) Mention the type of immune response provided by an antibody. 26. Describe the elongation process of transcription in bacteria. SECTIOND

121

29. Explain the causes, inheritance pattern and symptoms of any two Mendelian genetic disorders. OR (i) Natural selection operates when nature select for fitness. Explain. (ii) The rate of appearance of new forms is linked to the lifespan of an organism. Explain with the help of a suitable example.

122

Xam idea BiologyXII

SETIII
(Uncommon Questions to Set I and II) SECTIONA 2. How do animals like fish and snails avoid summer related unfavourable conditions? 3. In a pond there were 200 frogs. 40 more were born in a year. Calculate the birth rate of the population. SECTIONB 9. Where does triple fusion take place in a flowering plant? Why is it so called? Mention its significance. 10. Why certain regions have been declared as biodiversity hot spots by environmentalists of the world? Name any two hot spot regions of India. 13. Study the given aquatic food chain and answer the questions that follow:
Fish eating birds (DDT 5 ppm)

Large fish (DDT 2 ppm)

Small fish (DDT 0.5 ppm)

Zooplankton (DDT 0.04 ppm)

Water (DDT 0.003 ppm)

(i) Give reasons why there is a continuous increase in the DDT content in different trophic levels of the chain. (ii) Name the phenomenon responsible for the increase in DDT content. 17. Why are F2 phenotypic and genotypic ratios same in a cross between red-flowered snapdragon and white-flowered snapdragon plants. Explain with the help of a cross.

Examination Papers SECTIONC 20. Describe the termination process of transcription in bacteria. 26. An antibody molecule is represented as H2L2. Explain. SECTIOND

123

30. . Write the symptoms of haemophilia and sickle-cell anaemia in humans. Explain how the inheritance pattern of the two diseases differs from each other. OR (a) Write the Hardy-Weinberg principle. (b) Explain the three different ways in which natural selection can affect the frequency of a heritable trait in a population shown in the graph given below.
Medium-sized individuals are favoured

Number of individuals with phenotype

Phenotypes favoured by natural selection

Trait

124

Xam idea BiologyXII

SOLUTIONS
SETI SECTIONA 1. The bilobed anther can produce 1600 male gametophytes. 2. Two functions of the codon AUG are: (i) It acts as a start codon during protein synthesis. (ii) It codes for the amino acid methionine. 3. Louis Pasteur disproved the theory of spontaneous generation. 4. The immunological memory induced by the vaccine in a child prevents the recurrence of a disease. 5. Plant cells have cellulose in their cell wall which need to be degraded using the enzyme cellulase for isolating genetic material. On the other hand, animal cell wall do not contain cellulose and hence, cellulase is not required. 6. ELISA (Enzyme Linked Immunosorbent Assay) can be used to detect the presence of a pathogen. No. of individuals dead 8 7. Death rate = = = 0.1 Total no. of individuals 80 The death rate will be 0.1 individuals per week. 8. A positive application of amniocentesis is that it helps in diagnosing genetic disorders. A negative application of amniocentesis is that it helps in identifying the sex of the foetus which can lead to female infanticide. SECTIONB 9. Antherozoids are motile male gametophytes which have to swim on the water surface to fertilize the immotile female gametophytes, i.e., egg. Since, during its transfer many antherozoids get destroyed, a large number of them are produced. 10. A zygote is formed by the fusion of haploid male gamete with the haploid egg to form a diploid cell; where as, Primary Endosperm Nucleus (PEN) is formed by the fusion of haploid male gamete with two haploid polar nuclei, producing a triploid nucleus. 11. Electrostatic Precipitator (ESP) l It is an electrical device that removes more than 99 per cent particulate matter present in the industrial exhaust from a thermal power plant. l ESP has electrode wires and a stage of collecting plates. l Electrode wires are given an electric current of several thousand volts, which produces a corona that releases electrons. l These electrons attach to dust particles and give them a negative charge within a very small fraction of a second. l Collecting plates are earthed that attract charged dust particles.

Examination Papers

125

Discharge corona corona Negatively charged charged particle particle

Dirty air air

Clean air air

Dust particles particles Collection plate plate grounded grounded Electrostatic precipitator precipitator

Electrostatic precipitator

12. In aquatic system, detritus food chain and in terrestrial ecosystem, grazing food chain are responsible for flow of larger fraction of energy. Types of food chains: Grazing Food Chain (GFC) (i) It starts with green plants called producers as first trophic level. Energy for food chain comes from the Sun. Detritus Food Chain (DFC) It begins with dead organic matter and decomposers called saprophytes as first trophic level. Energy for the food chain comes from organic remain or detritus.

(ii)

13. In a typical test cross an organism showing a dominant phenotype (and whose genotype is to be determined) is crossed with the recessive parent. The progenies of the cross are analysed to predict the genotype of the test organism. E.g., following is a test cross where violet colour flower of pea (W) is dominant over white colour flower (w). Case I Parents Gametes Progeny w w WW ww W w W Ww Ww W Ww Ww w w Inference: Unknown flower is homozygous dominant. Case II Ww ww W w w All flowers violet W Ww Ww w ww ww

Unknown flower is heterozygous dominant.

126 14. Host (a) Formation of gametocytes (b) Fusion of gametocytes Human Anopheles mosquito

Xam idea BiologyXII

Site of Occurrence Red blood cells Intestinal lining

15. Honeybees are good pollinators of almost all the plants. The flowers in turn offer floral rewards like nectar and pollen grains. So, when beehives are kept in crop-fields during the flowering season, honey collection increases. OR A small amount of curd contains Lactobacillus and other Lactic Acid Bacteria (LAB) that grow in milk and convert milk into curd. LAB produces lactic acids that cause coagulation of protein in milk to from curd. LAB increases vitamin B12 along with other vitamins in the curd. 16. Introduction of genetically engineered lymphocytes into a ADA deficiency patient is not a permanent cure because these cells are not immortal and the patient requires periodic infusion of such genetically engineered lymphocytes. A possible permanent cure can be isolating the gene producing Adenosine Deaminase (ADA) from bone marrow cells and introducing it into cells at early embryonic stages. 17. The orchid bears flowers which resemble the female wasp in colour, smell as well as appearance. The male pollinators mistake them as their female counterparts. In their process of their pseudocopulating, they pollinate the flower. 18. Two levels of biodiversity are: (i) Species diversity: It is the diversity at species level. Eg., amphibian species are found more in the Western Ghats as compared to the Eastern Ghats. (ii) Genetic diversity: A single species shows high diversity at the genetic level over its distributional range. Eg., Different genetic varieties of Rauwolfia vomitoria are found on the Himalayas.

Examination Papers SECTIONC 19.

127

Pollen tube Central cell Antipodal Polar nuclei Egg cell Synergid Egg nucleus Plasma membrane Synergid Filiform apparatus Male gametes Vegetative nucleus (b)

(a)

(a) L. S. of pistil showing path of pollen tube growth; (b) enlarged view of an egg apparatus showing entry of pollen tube into a synergid.

OR

Spermatozoa Spermatid Secondary spermatocyte Primary spermatocyte Sertoli cell

Spermatogonium

Diagrammatic sectional view of seminiferous tubule (enlarged)

20.

(i) T. H. Morgan carried out several dihybrid crosses in Drosophila to study the genes that are sex-linked. He observed that when the two genes in a dihybrid cross are located on the same chromosome, the proportion of parental gene combinations in the progeny was much higher than the non-parental or recombination of genes. (ii) Morgan and his group found that when genes were grouped on the same chromosome, some genes are tightly linked and show low recombination. (iii) When the genes are loosely linked they show higher recombination. (iv) Morgan hybridised yellow bodied and white eyed females with brown bodied and red eyed males (wild type) (cross-A) and inter-crossed their F1 progeny.

128 The F2 generation contained as given in Figure below. Cross A

Xam idea BiologyXII

Cross B

y Parental

y+ w+

w+

m+

y w Yellow, white

Wild type

w m White, miniature

Wild type

y F1 generation

y+ w+ Wild type

Yellow, white Gametes

w+ m+ Wild type

White, miniature

Parental type (98.7%) y+ w+

Recombinant type (1.3%) y+ w

Parental type (62.8%) w+ m+

Recombinant type (37.2%) w+ m

Wild type y w y

White w+ w

Wild type m

miniature w m+

Yellow, white F2 generation y

+

Yellow

White, mintature

White

y y y y

w w White w+

w w w

y w Wild type y w

m Wild type m

w m Miniature w m+

y w Yellow, white

w Yellow

w m White, miniature

m White

Linkage: Results of two dihybrid crosses conducted by Morgan. Cross A shows crossing between genes y and w; Cross B shows crossing between genes w and m. Here dominant wild type alleles are represented with (+) sign in superscript.

Examination Papers

129

21. In bacteria, the transcription of all the three types of RNA (mRNA, tRNA, rRNA) is catalysed by single DNA-dependent enzyme, called the RNA polymerase. The RNA polymerase has co-factors b, b, a, a and w along with s (sigma ) factor that catalyse the process. During initiation, s factor recognises the start signal and promoter region on DNA, and the (Sigma) with RNA polymerase binds to the promoter.
3' Initiation 5' Promoter RNA polymerase s Sigma factor DNA helix 5' 3'

Process of transcription initiation in bacteria

22. Convergent evolution: When more than one adaptive radiation appeared to have occurred in an isolated geographical area and two or more groups of unrelated animals resemble each other for similar mode of life or habitat; it is called convergent evolution; eg., Australian marsupials, placental mammals. Divergent evolution: In some animals, the same structure developed along different directions due to adaptions to different needs. This is known as divergent solution; eg., forelimbs of whales, bats, cheetah and human, perform different functions as they have similar anatomical structure. 23. On its entry into the body, HIV attacks macrophages. Lifecycle of HIV l After getting into the body of a person, the virus enters into macrophages, where RNA replicates and forms viral DNA by the help of enzyme reverse transcriptase.
l

The viral DNA gets incorporated into the host cells DNA and directs the infected cells to produce viruses. The macrophages continue to produce virus that enters the helper T-lymphocytes. Thus, the number of helper T-lymphocytes progressively decreases in the body of the infected persons.

l l

Thus, the person suffers from various pathogens like Mycobacterium, viruses and parasites like Toxoplasma. 24. The following efforts need to be put in:
l

(i) The cattle in the dairy farm must be housed and fed properly. (ii) Cleanliness should be maintained in the milking area. (iii) The health of the dairy cattle should be of utmost importance and a veterinary doctor must be invited regularly. (iv) Regular inspections of the farm, maintaining, records, identification and rectification of problems should be done along with maintaining precautionary measures. (v) Milking should be done in a dirt-free area and all the sanitary conditions should be maintained. (vi) High-yielding and disease-resistant breeds can be selected to maximise benefits

130 25. (a) Statins (c) Penicillium notatum (e) Trichoderma polysporium 26. (b) Blocks cholesterol lowering agent. (d) Penicillin (f) Immunosuppressant

Xam idea BiologyXII

The enzyme cuts both DNA strands at the same site Vector DNA
G A C T A T T A T A C G

EcoRI cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA Foreign DNA
G A C T A T T A T A C G

EcoR1 Sticky end

G C T T A A

C A A T T G

Sticky end DNA fragments join at sticky ends

Recombinant DNA

Steps in formation of recombinant DNA by action of restriction endonuclease enzyme EcoRI

27. The nematode Meloidegyne incognitia infects tobacco roots leading to reduction in the yield. To prevent this infestation, a strategy based on the process of RNA interference (RNAi) was adopted. Using Agrobacterium vectors, nematode-specific genes were introduced into host plants. This DNA produced both sense and anti-sense RNA in the host cells. These two RNAs being complementary to each other, formed a double stranded (dsRNA) RNA. The specific mRNA of the nematode binds to dsRNA and thus prevents its translation (silencing). The parasite could not survive in a transgenic host expressing specific interfering RNA. And the transgenic plant is thus, protected.

Examination Papers SECTIOND 28. (i) S. No. (i) Mendelian Disorder This disorder is mainly due to alteration or mutation in the single gene. This follows Mendels principles of inheritance. This may be recessive or dominant in nature For example, haemophilia, sickle-cell anaemia. Chromosomal Disorder

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This disorder is caused due to absence or excess or abnormal arrangement of one or more chromosomes. This does not follow Mendels principles of inheritance. This is always dominant in nature. For example, Turners syndrome.

(ii) (iii) (iv)

(ii) Two chromosomal aberration-associated disorders are Downs syndrome and Klinefelters syndrome. (iii) (a) Downs syndrome: The individuals have overall masculine development but they express feminine development like development of breast, i.e., gynaecomastia. They are sterile. (b) Klinefelters syndrome: The females are sterile as ovaries are rudimentary. Other secondary sexual characters are also lacking. OR Fitness is based on certain characteristics which are inherited and the ability to adapt to the changing environment. It is the end result of adaptation because a fit individual survives and unfit individuals are eliminated from the population. Individuals continuously compete with each other in a population for food, space and light. The one which is better adapted and naturally selected by nature survives and reproduces. For example, industrial evidence: It is a case of natural selection. In England, it was observed before industrialisation that white-winged moth were more than dark-winged moth. But the situation became reversed after industrialisation. It was found that predators will spot and pick a moth against a contrasting background. During pre-industrialisation, the tree trunks were covered by white lichens and on white-background dark coloured moth can be picked up. During post industrialisation, the tree trunks were covered by dark, dust, coal particles and became dark, on which white moth can easily be picked up. Thus, it was found that industrial melanism supports evolution by natural selection. 29. Oogenesis l The process of formation of a mature female gamete or ova in the ovary is known as oogenesis. l Germ cells of female foetus divide to produce oogonia which later grow into ovarian follicles.

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l l

l l

Oogonia start dividing and enter into prophase-I of the meiotic division and get temporarily arrested at that stage, called primary oocytes. Primary oocytes are enclosed by a layer of granulosa cells, the follicle cells, and known as primary follicle. The primary follicles get surrounded by more layers of granulosa cells and a thecal layer known as secondary follicles. The secondary follicle soon transforms into a tertiary follicle which is characterised by a fluid-filled cavity called antrum. Primary oocyte undergoes first meiotic division and produces two haploid cells: a large haploid secondary oocyte and a small first polar body. Secondary oocyte moves into the fallopian tube. When a sperm enters its cytoplasm, secondary oocyte completes meiosis-II. The secondary oocyte undergoes the second meiotic division and produces a mature ovum and a second polar body. Polar bodies have no function and eventually degenerate. Mature follicle or Graafian follicle ruptures to release the ovum from the ovary by the process called ovulation.
CHROMOSOMES NUMBER PER CELL 46 Oogonia Mitosis differentiation

Fetal life Primary oocyte 1 st meiotic division (completed prior to ovulation)

Birth Childhood Puberty 23 First polar body 23 Adult reproductive life Second polar body

Secondary oocyte

Ovum

Systematic representation of oogenesis

Effect of gonadotropins: l The primary follicles in the ovary grow and become a fully mature Graafian follicle. l The endometrium of the uterus is regenerated due to the secretion of LH and FSH from anterior pituitary and ovarian hormone, estrogen. It lasts for about 14 days.

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Rapid secretion of LH (LH surge) induces rupture of Graafian follicle and thereby leading to ovulation (release of ovum). OR

(i) Fertilisation The process of fusion of a sperm (male gamete) with an ovum (female gamete) is called fertilisation. l During coitus, semen is released by the penis into the vagina (insemination). l The motile sperms swim rapidly, through the cervix, enter into the uterus and reach the ampullaryisthmic junction of the oviduct. l A sperm comes in contact with the zona pellucida layer of the ovum and induces changes in the membrane to block the entry of additional sperms. l The enzymes of the acrosome of sperm help to dissolve zona pellucida and plasma membrane of the ovum and sperm head is allowed to enter into the cytoplasm of the ovum, i.e., secondary oocyte. l Ultimately, diploid zygote is produced by the fusion of a sperm and an ovum. (ii) Implantation l Zygote divides rapidly by mitotic division called cleavage and as a result 2, 4, 8, 16 daughter cells are produced which are termed as blastomeres. l Embryo with 816 blastomeres is called a morula. l The morula changes into a large mass of cells called blastocyst which passes further into the uterus. l Blastomeres in the blastocyst are arranged into an outer layer called trophoblast and an inner group of cells attached to trophoblast called inner cell mass.
l

Sperm

Zona pellucida

Ovum

Cells of the corona radiata

Perivitelline space

Ovum surrounded by few sperms

134

Xam idea BiologyXII The trophoblast layer gets attached to the cells of the endometrium and the inner cell mass gives rise to the embryo. l The cells of endometrium divide rapidly and cover the blastocyst. l So, the blastocyst gets embedded in the endometrium of the uterus called implantation leading to pregnancy. (iii) The embryonic stage that gets implanted is called blastocyst.
l

(a)

(b) C B D

(c) E

(d)

(Morula) (e)

F A G H Blastocyst Implantation

(f)

(Blastocyst) (g)

Transport of ovum, fertilisation and passage of growing embryo through fallopian tube

30. Logistic growth

Population density (N)

b dN = rN KN K dt

( )

Time (t)

Population growth curve When responses are limiting the growth, plot is logistic, K is carrying capacity

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135

l l

The resources become limited at certain point of time, so no population can grow exponentially. Every ecosystem or environment or habitat has limited resources to support a particular maximum number of individuals called its carrying capacity (K). When N is plotted in relation to time t, the logistic growth show sigmoid curve and also called Verhulst-Pearl logistic Growth. K- N dN / dt = rN , K Where N = Population density at time t r = Intrinsic rate of natural increase

K = Carrying capacity. OR The process of breaking down complex organic matter into inorganic substances likeCO 2 , water and nutrient is called decomposition. The raw materials for decomposition is called detritus. They are dead remains of plants and animals. Steps in decomposition: (a) Fragmentation: The process of breaking down of detritus into smaller particles is called fragmentation, e.g., earthworm. (b) Leaching: The process by which water-soluble inorganic nutrients go down into the soil horizon and get precipitated as unavailable salts. (c) Catabolism: The enzymatic process by which degraded detritus is converted into simple inorganic substances is called catabolism. (d) Humification: The process of accumulation of a dark coloured amorphous substance called humus, that is, highly resistant to microbial action and undergoes decomposition at an extremely slow rate. (e) Mineralisation: The process by which humus is further degraded by some microbes and release inorganic nutrients is called mineralisation.

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SETII
SECTIONA 6. A malfunctioning tapetum is not able to provide optimum nutrition for the production of viable male gametophytes. 8. Birth rate = No. of individuals born = 10 = 0.2 plants per year. Total no. of individuals 20 SECTIONB 9. The two ecosystem services are: (i) Forest ecosystem purify air, mitigate droughts and floods. (ii) The cycle nutrients generate fertile soil and maintains biodiversity. 14. Mature female gametophyte have 7 haploid cells which are 2 synergids, 1 egg cell, 3 antipodal cells and one central cell. 15. Production of one-fourth dwarf progeny on selfing of a tall pea plant indicates that the plant is heterozygous. This can be explained with the cross as below: Parents: Gametes: T Progeny: Tt t T Tt t (on selfing)

Half flowers violet and half flowers white T T t TT Tall Tt Tall t Tt Tall tt Dwarf

16. CFCs find wide use as refrigerants. CFCs discharged in the lower part of atmosphere move upward and reach stratosphere. In stratosphere, UV rays act on them releasing Cl atoms. Cl degrades ozone releasing molecular oxygen. Whatever CFCs are added to the stratosphere have permanent and continuing effects on ozone. SECTIONC 22. (a) Antigen binding site (b) Light peptide chain (c) Heavy peptide chain (i) Antibody is an immunoglobulin (ii) B-Cells (iii) Humoral immune response 26. After initiation of RNA transcription, RNA polymerase lose the s factor. It uses nucleoside triphosphates as substrate and polymerises in a template depended fashion following the rule of complementarity. It somehow also facilitates opening of the helix and continues elongation. This is the process of elongation.

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Process of transcription elongation in bacteria

SECTIOND 29. Haemophilia (i) It is a sex-linked recessive disorder. (ii) Patient continues to bleed even on a minor cut as the patient does not possess natural phenomenon of blood clotting. (iii) The gene for haemophilia is located on X-chromosome. (iv) So more males suffer from haemophilia than females because in males single gene for the defect is able to express. (v) The defective alleles produce non-functional protein which later form a non-functional cascade of protein involving in blood clotting. (vi) Females suffer from this disease only in homozygous condition, i.e., Xc Xc . Sickle-cell Anaemia (i) It is an autosomal linked recessive trait. (ii) The disease is controlled by a single pair of allele Hb A and Hb S . (iii) Only the homozygous individuals for Hb S , i.e., Hb S Hb S show the diseased phenotype. (iv) The heterozygous individual are carriers (Hb A Hb S ). (v) Due to point mutation Glutamic acid (Glu) is replaced by Valine (Val) at sixth positions of beta globin chain of haemoglobin molecule. (vi) Hb S behaves as normal haemoglobin except under oxygen stress where erythrocytes lose their circular shape and become sickle-shaped. As a result, the cells cannot pass through narrow capillaries. Blood capillaries are clogged and thus affects blood supply to different organs. OR (i) Natural resources are limited, populations are stable in size, members of a population vary in characteristics even though they look superficially similar. Theoretically, population will increase exponentially but the population sizes in reality are limited thus leading to competition. Only the ones which are fit and adapt themselves are able to survive. They grow at the cost of others and flourish. This was called as natural selection by Darwin. (ii) According to Darwin, the fitness of an organism is measured by its reproductive ability. Also the appearance of new forms is linked to the lifespan of an organism. The greater its lifespan, the more it can reproduce and hence, greater new forms would appear. This can be observed in the Development of dark-winged moth due to industrial melanism. For example, industrial evidence: It is a case of natural selection. In England, it was observed before industrialisation that white-winged moth were more than dark-winged moth.

138

Xam idea BiologyXII But the situation became reversed after industrialisation. It was found that predators will spot and pick a moth against a contrasting background. During pre-industrialisation, the tree trunks were covered by white lichens and on white-background dark coloured moth can be picked up. During post industrialisation, the tree trunks were covered by dark, dust, coal particles and became dark, on which white moth can easily be picked up. Thus, it was found that industrial melanism supports evolution by natural selection.

SETIII
SECTIONA 2. Fish and snails undergo aestivation i.e., summer sleep to avoid unfavourable conditions. No. of individuals born 40 3. Birth rate = = 0.5 frogs per year. = Total no. of individuals 200 SECTIONB 9. Triple fusion: It is a fertilisation that involves fusion of one male gamete and two polar nuclei (or secondary nucleus; if the two have already fused) in the central cell of embryo sac. It takes place in the central cell of an embryo sac. Three nuclei are involved in triple fusion, i.e., one male nucleus and two polar nuclei in the central cell therefore, the process is termed triple fusion. Significance: Endosperm is triploid and helps in providing nourishment to the embryo. 10. Certain regions have been declared as hot spots for maximum protection of these regions which have high levels of species richness and high degree of endemism. Western Ghats and Himalayas are two example of hot-spots. 13. (i) When DDT enters the food chain then they reach water. Since they are not degradable these get accumulated progressively at each trophic level. As they reach higher trophic levels, the concentration of DDT also increases.

(ii) Biological magnification. 17. This is a case of Mendelian deviation and inheritance that shows incomplete dominance as red and white colour traits both are not expressed and produce pink trait in F1 generation. Parents Red flower White flower RR rr Gametes R Rr On selfing Rr Gametes R r R Rr r r (All red flowers)

F1 generation

Examination Papers R F2 generation R r Phenotypic ratio: Genotypic ratio: Red 1 RR 1 : : : : Pink : 2 : RR Red Rr Pink White 1 r Rr Pink rr White

139

Rr : rr 2 : 1 SECTIONC

20. Termination: Once the RNA polymerase reaches the termination region of DNA, the RNA polymerase is separated from DNA-RNA hybrid, as a result nascent RNA separates. This process is called termination which is facilitated by certain termination factor r. In prokaryotes, mRNA does not require any processing, so transcription and translation occurs both in the cytosol. It can be said that the transcription and translation are coupled together.
3' 5' Termination RNA r 5' 3' RNA Polymerase Rho factor

26. An antibody molecule consists of four polypeptide chains, two are long called heavy (H) chains while other two are short called light (L) chains. Both are arranged in the shape of Y. Hence, the antibody is represented as H 2 L 2 . SECTIOND 30. Symptoms of Haemophilia. (i) Patient continues to bleed even on a minor cut as the patient does not possess natural phenomenon of blood clotting. Symptoms of Sickle-cell Anaemia. (i) Hb S behaves as normal haemoglobin except under oxygen stress where erythrocytes lose their circular shape and become sickle-shaped. As a result, the cells cannot pass through narrow capillaries. Blood capillaries are clogged and thus affects blood supply to different organs. Differences between Haemophilia and Sickle-cell Anaemia. Haemophilia (i) (ii) It is a sex-linked recessive disorder. The gene for haemophilia is located on X-chromosome. Sickle-cell Anaemia It is an autosomal linked recessive trait. The disease is controlled by a single pair of allele Hb A and Hb S .

140

Xam idea BiologyXII More males suffer from haemophilia than Only the homozygous individuals for Hb S , females because in males single gene for i.e., Hb S Hb S show the discased the defect is able to express. Females suffer phenotype. from this disease only in homozygous condition, i.e., Xc Xc .

(iii)

(iv) The defective alleles produce non-functional Due to point mutation Glutamic acid (Glu) protein which later form a non-functional is replaced by Valine (Val) at sixth cascade of protein involving in blood positions of beta globin chain of clotting. haemoglobin molecule. OR (a) Hardy-Weinberg Principle: Hardy-Weinberg principle states that the gene pool (total genes and their alleles in a population) remains constant, i.e., the allele frequencies in a population are stable and constant from generation to generation. This biological phenomenon is called genetic equilibrium. (b) Natural selection can lead to stabilisation (in which more individuals acquire mean character value), directional change (more individuals acquire value other than the mean character value) or disruption (more individuals acquire peripheral character value at both ends of the distribution curve).
Peak gets higher and narrow

(a) Number of individuals with phenotype Phenotypes favoured by natural selection Medium-sized individuals are favoured Peak shifts in one direction

Trail

(b) two peaks form

(c)

Diagrammatic representation of the operation of natural selection of different traits: (a) stabilising (b) Directional and ( c) Disruptial.

EXAMINATION PAPERS 2010

CBSE (ALL INDIA)

Time allowed: 3 hours General Instructions: (i) All questions are compulsory.

Max. Marks: 70

(ii) This question paper consists of four sections A, B, C and D. Section A contains 8 questions of one mark each, Section B is of 10 questions of two marks each, Section C is of 9 questions of three marks each and Section D is of 3 questions of five marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.

SETI
SECTIONA 1. Mention the site where syngamy occurs in amphibians and reptiles respectively. 2. How is snow-blindness caused in humans? 3. Name one autosomal dominant and one autosomal recessive Mendelian disorder in humans. 4. How is the action of exonuclease different from that of endonuclease? 5. India has more than 50,000 strains of rice. Mention the level of biodiversity it represents. 6. Mention the information that the health workers derive by measuring BOD of a water body. 7. Name the enzyme involved in the continuous replication of DNA strand. Mention the polarity of the template strand. 8. Offsprings derived by asexual reproduction are called clones. Justify giving two reasons. SECTIONB 9. Mention the role of ribosomes in peptide-bond formation. How does ATP facilitate it? 10. How do copper and hormone releasing IUDs act as contraceptives? Explain. 11. If you squeeze a seed of orange you might observe many embryos of different sizes. How is it possible? Explain. 12. A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. Explain how the sticky ends are formed and get joined.

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Xam idea BiologyXII

13. (i) Mention the number of primers required in each cycle of polymerase chain reaction (PCR). Write the role of primers and DNA polymerase in PCR. (ii) Give the characteristic feature and source organism of the DNA polymerase used in PCR. 14. Define the term 'health'. Mention any two ways of maintaining it. OR Why does a doctor administer tetanus antitoxin and not a tetanus vaccine to a child injured in a roadside accident with a bleeding wound? Explain. 15. Giving two reasons explain why there is more species biodiversity in tropical latitudes than in temperate ones. 16. Name an opioid drug and its source plant. How does the drug affect the human body? 17. Mention the major cause of air pollution in metro cities. Write any three ways by which it can be reduced. 18. How did Eli Lilly synthesise the human insulin? Mention one difference between this insulin and the one produced by the human pancreas. SECTIONC 19. (i) Write the characteristic features of anther, pollen and stigma of wind pollinated flowers. (ii) How do flowers reward their insect pollinators? Explain. 20. (i) Why are grasshopper and Drosophila said to show male heterogamity? Explain. (ii) Explain female heterogamity with the help of an example. 21. In a series of experiments with Streptococcus and mice F . Griffith concluded that R-strain bacteria had been transformed. Explain. 22. (a) How does the Hardy-Weinberg's expression (p 2 + 2pq + q 2 = 1) explain that genetic equilibrium is maintained in a population? (b) List any two factors that can disturb the genetic equilibrium. 23. Mention the name of the causal organism, symptoms and the mode of transmission of the disease Amoebiasis. 24. (i) Mention the property that enables the explants to regenerate into a new plant. (ii) A banana herb is virus-infected. Describe the method that will help in obtaining healthy banana plants from this diseased plant. 25. Mention the product and its use produced by each of the microbes listed below: (i) Streptococcus (ii) Lactobacillus (iii) Saccharomyces cerevisiae 26. (i) Name the organism in which the vector shown in inserted to get the copies of the desired gene. (ii) Mention the area labelled in the vector responsible for controlling the copy number of the inserted gene. (iii) Name and explain the role of a selectable marker in the vector shown.

Examination Papers

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Cla I Eco R I Pvu I

Hind III

Bam H I Pst I ampR tetR Sal I pBR322

ori rop

Pvu II

OR Name the insect pest that is killed by the products of cryIAc gene. Explain how the gene makes the plant resistant to the insect pest. 27. How do organisms like fungi, zooplanktons and bears overcome the temporary short-lived climatic stressful conditions? Explain. SECTIOND 28. Describe in sequence the events that lead to the development of a 3-celled pollen grain from microspore mother cell in angiosperms. OR (a) Give a schematic representation showing the events of spermatogenesis in human male. (b) Describe the structure of a human sperm. 29. (a) State the law of independent assortment. (b) Using Punnett Square demonstrate the law of independent assortment in a dihybrid cross involving two heterozygous parents. OR How did Alfred Hershey and Martha Chase arrive at the conclusion that DNA is the genetic material? 30. (a) Why are herbivores considered similar to predators in the ecological context? Explain. (b) Differentiate between the following interspecific interactions in a population: (i) Mutualism and Competition (ii) Commensalism and Amensalism OR (a) Trace the succession of plants on a dry bare rock. (b) How does phosphorus cycle differ from carbon cycle?

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SETII
(Uncommon Questions to Set I) SECTIONA 2. Write the genotype of (i) an individual who is carrier of sickle cell anaemia gene but apparently unaffected, and (ii) an individual affected with the disease. 6. Mention a characteristic feature and a function of zoospores in some algae. SECTIONB 9. Placenta acts as an endocrine tissue. Justify. 10. Differentiate between menarche and menopause. 13. List the features that make a stable biological community. 17. Mention how e-waste is produced and disposed off. Write the solution for its treatment. SECTIONC 21. Explain the process of artificial hybridisation to get improved crop variety in (i) plants bearing bisexual flowers (ii) female parent producing unisexual flowers. 27. Explain the sex determination mechanism in humans. How is it different in birds? SECTIOND 30. (a) Trace the development of megaspore mother cell up to the formation of a mature embryo-sac in a flowering plant. (b) Draw a labelled diagram of the structure of mature dicot embryo. OR (a) Draw a diagrammatic labelled sectional view of a seminiferous tubule of a human. (b) Describe in sequence the process of spermatogenesis in humans.

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SETIII
(Uncommon Questions to Set I and II) SECTIONA 1. A human being suffering from Downs Syndrome shows trisomy of 21st chromosome. Mention the cause of this chromosomal abnormality. 4. Pea flowers produce assured seed sets. Give a reason. SECTIONB 13. Differentiate between major structural changes in the human ovary during the follicular and luteal phase of the menstrual cycle. 15. Alien species are a threat to native species. Justify taking examples of an animal and a plant alien species. 17. Explain giving reasons the cause of appearance of peaks a and b in the graph shown below.
a Dissolved oxygen b

CONCENTRATION

BOD

DIRECTION OF FLOW Sewage discharge

Effect of sewage discharge on some important characteristics of a river 18. Explain the zygote intra fallopian transfer technique (ZIFT). How is intrauterine transfer technique (IUT) different from it? SECTIONC 20. Explain the mechanism of sex determination in insects like Drosophila and grasshopper. 27. (a) Mention any four strategies adopted by flowering plants to prevent self-pollination. (b) Why is geitonogamy also referred to as genetical autogamy? SECTIOND 29. (a) Draw a labelled longisectional view of albuminous seed. (b) How are seeds advantageous to flowering plants? OR Describe the post-zygotic events loading to implantation and placenta formation in humans. Mention any two functions of placenta.

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SOLUTIONS
SETI SECTIONA 1. In amphibians, external fertilisation occurs hence, syngamy occurs in the medium of water. In reptiles, internal fertilization occurs hence, syngamy occurs within the body. 2. In human eye, cornea absorbs UV-B radiation, and a high dose of UVB causes inflammation of cornea causing snow-blindness. 3. Huntingtons disease is an autosomal dominant disorder and Haemophilia is an autosomal recessive disorder. 4. Exonucleases leave the DNA molecules at their ends whereas endonucleases leave DNA molecules internally. 5. 50,000 strains of rice represent specific biodiversity. 6. By measuring BOD of a water body, health workers find the amount of dissolved oxygen in water. The lesser the amount of dissolved oxygen, the more polluted the water body will be. 7. DNA polymerase is involved in continuous replication of DNA strand. The polarity of template strand is 35. 8. Offsprings derived by asexual reproduction are called clones because they are identical to the parent and also to each other. SECTIONB 9. There are two sites in the large subunit of the ribosome, for subsequent amino acids to bind to and thus, be close enough to each other for the formation of a peptide bond. The ribosome also acts as a catalyst for the formation of peptide bond 23S rRNA in bacteria is a ribozyme. Amino acids become activated by binding with its aminoacyltRNA synthetase in the presence of ATP . 10. The copper releasing IUDs release Cu ions which supress sperm motility and the fertilising capacity of sperms. The hormone releasing IUDs make the uterus unsuitable for implantation and the cervix hostile to the sperms. 11. In orange, the nucellar cells surrounding the embryo sac start dividing, protrude into the embryo sac and develop into a number of embryos of different sizes. 12. The vector DNA and foreign DNA are cut by the same restriction enzyme like EcoRI to form the same kind of sticky-ends. Then these sticky ends are joined by the enzyme DNA ligase.

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The enzyme cuts both DNA strands at the same site Vector DNA
G A C T A T T A T A C G

EcoRI cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA Foreign DNA
G A C T A T T A T A C G

EcoR1 Sticky end

G C T T A A

C A A T T G

Sticky end DNA fragments join at sticky ends

Recombinant DNA

Steps in formation of recombinant DNA by action of restriction endonuclease enzyme EcoRI

13.

(i) Each cycle of PCR requires two sets of primers. Role of Primers: Primers are complementary to the regions of DNA. They anneal to both the strands of DNA which are then extended using nucleotides. Role of DNA Polymerase: (i) The enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template.

(ii) DNA polymerase used in PCR is thermostable and is called Taq polymerase. It is obtained from the bacterium Thermus aquaticus. 14. Health is a state of complete physical, mental and social well-being. Good health is maintained by balanced diet, personal hygiene and regular exercise, yoga, vaccination against infectious diseases, proper disposal of wastes, control of vectors, etc. OR Tetanus is caused by a microbe which has a deadly and fast action. Action of vaccine is slow and this delay may become fatal. Therefore, antitoxins are administered which show an instant response against tetanus toxin. 15. The reasons are: (i) Tropical environments unlike temperate ones, are less seasonal, relatively more constant and predictable which promote greater species diversity. (ii) There is more solar energy available in the tropics which contribute to higher productivity leading to greater diversity.

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16. Heroin is an opioid drug obtained from the poppy plant Papaver Somniferum. It is a depressant and slows down the body functions. 17. Major causes of air pollution are: (i) Decomposition of garbage resulting in release of unwanted gases into the atmosphere. (ii) Burning of fossil fuels in automobiles and industries, release particulate and air pollutants. (iii) Use of leaded petrol. (iv) Chemical industries including pesticides, fertilisers and fungicides, etc. Control methods: (i) Using electrostatic precipitators to remove particulate matter from exhaust of industries and thermal power plant. (ii) Using scrubber to remove gases like SO2. (iii) Use of catalytic converters in automobiles for reducing emission of poisonous gases. 18. Eli Lilly prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin. Insulin in human pancreas is synthesized as a prohormone containing the C peptide. Which is removed in mature hormone. The synthesised insulin did not contain C peptide and was prepared in mature form. SECTIONC 19. (i) The characteristics are: (a) Pollen grains are light in weight, non- sticky, dry and winged. (b) Well-exposed stamens for easy dispersal of pollen grains in the wind. (c) The stigma is sticky, large, feathery to trap pollen grains in air. (ii) Insect pollinators are rewarded in following ways: (a) The flowers offer floral reward like nectar and pollen grain. (b) In some species floral reward provides safe place to lay eggs. 20. (i) Drosophila exhibit XY type of sex determination. Males produce two types of sperms, one having X chromosome and one having Y chromsome. Whereas females have only X-type of chromosomes. Grasshoppers exhibit XO type of sex determination. Males produce two types of gametes, one with X chromosome and other with no chromosome. Thus, both show male heterogamety.

(ii) Female heterogamety can be seen in female birds. In these, the females have one Z and one W chromosome whereas males have a pair of Z-chromosomes besides the autosomes. 21. F. Griffith (1928), conducted an experiment with Streptococcus pneumoniae (bacterium causing pneumonia). He observed two strains of this bacterium, one forming a smooth shiny colony (S-type) with capsule, while other forming rough colonies (R-type) without capsule. When live S-type cells were injected into the mice, mice died due to pneumonia. When live R-type cells were injected into the mice, mice survived. When heat killed S-type cells were injected into the mice, mice survived and there was no symptoms of pnuemonia. When heat killed S-type cell were mixed with live R-type cells and injected into the mice, the mice died due to unexpected

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symptoms of pneumonia. He concluded that heat killed S-type bacteria caused a transformation of the R-type bacteria into S-type bacteria.

virulent S-cells

Non-Virulent R-cells

Heat Killed S-cells

Heat Killed S-cells + Live R-cells

Died

Survived

Survived

Died

Absence of bacteria Virulent (S-cells)

Absence of bacteria Virulent (S-cells)

Griffiths Experiment on Transformation

22. (a) (i) Sum Total of All the Allele Frequencies is 1: Let there be two alleles A and a in a population. The frequencies of alleles A and a are p and q respectively. The frequency of AA individual in a population is p 2 and it can be explained that the probability that an allele A with a frequency of p appear on both the chromosome of a diploid individual is simply the product of the probabilities, i.e., p 2 . Similarly, the frequency aa is q 2 and that of Aa is 2 pq. p 2 + 2 pq + q 2 = 1, where p 2 represents the frequency of homozygous dominant genotype, 2 pq represents the frequency of the heterozygous genotype and q 2 represents the frequency of the homozygous recessive. (ii) Genetic equilibrium states the status of evolutionIf there is some fluctuation or disturbance in genetic equililbrium or HardyWeinberg equilibrium, i.e., change of frequencies of alleles in a population then it can predicted that evolution is in progress. (b) Factors that affect HardyWeinberg equilibrium: (i) Gene migration or gene flow (ii) Genetic drift (iii) Mutation

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23. Causes: l It is caused by an intestinal endoparasite, Entamoeba histolytica found in large intestine of humans. l Housefly acts as mechanical carrier and transmits the parasite from faeces of infected person to the food. l Infection takes place through contaminated food and water. Symptoms: (a) Abdominal pain (b) Constipation (c) Cramps (d) Stool with excess mucous and blood clots, etc. 24. (i) Totipotency which is the ability of a cell to grow or generate the whole plant. (ii) Healthy banana plants can be obtained from diseased plants by meristem culture. Although the plant is virus infected, the meristem apical and axillary is free of virus. The meristem is removed from the plant and grown in vitro by micropropagation. The plants produced are virus-free. 25. Microbes (i) (ii) (iii) 26. Streptococcus Lactobacillus Saccharomyces cerevisiae Products Streptokinase Lactic acid Ethanol Use bioactive molecule used to remove blood clot. Coagulate and partially digest milk proteins and form curd. for bread-making, fermenting malted cereals and fruit juices.

(i) Escherichia coli (ii) Origin of replication ori controls copy number of inserted gene. (iii) The selectable markers are ampR (resistance to ampicillin)and tetR (resistance to tetracycline). Selectable markers help to select the host cells which contain the vector (transformants) and eliminating non-transformants. If a foreign DNA ligates at the BamHI site of tetracycline resistance gene in the vector pBR322, the recombinant plasmid loses the tetracycline resistance due to insertion of DNA. It can still be selected out from non-recombinant. OR
l

l l

Cotton bollworm are killed by products of cry IAc gene. Some strains of Bacillus thuringiensis produce proteins that kill some insects like lepidopterans, (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes). B. thuringiensis forms protein crystals which contain a toxic insecticidal protein. Bt toxins are initially inactive protoxins but after ingestion by the insect, their inactive toxin becomes active due to the alkaline pH of the gut which solubilise the crystals.

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The activated toxin binds to the surface of midgut epithelial cells thus creating pores which causes cell swelling and lysis further leading to death of the insects. l Specific Bt toxin genes obtained from Bacillus thuringiensis are used in several crop plants like cotton. l The toxin is coded by a gene called cry which are of various types. Example: proteins encoded by the genes cryIAc and cryIIAb control the cotton bollworms, that of cryIAb controls corn borer. 27. Fungi: They form thick-walled spores which help them survive in unfavourable conditions. On availability of suitable environment, these germinate.
l

Zooplankton: Under unfavourable conditions, these species in lakes and ponds enter diapause, a stage of suspended development. Bears: In extreme low temperatures, they escape winter time, i.e., they hibernate. SECTIOND 28. Microsporogenesis (i) The interior of the microsporangium is filled with sporogenous cells. These sporogenous cells divide meiotically to form microspore tetrads. (ii) So, the cells of sporogenous tissue are called pollen or microspore mother cell (PMC) because they undergo meiosis to produce pollen grains. (iii) The process of formation of microspore from a pollen mother cell through meiosis is called microsporogenesis. (iv) The microspores are developed from PMC in cluster of four due to meiosis called microspore tetrad. (v) When the anther matures and dehydrates, the microspores differentiate into pollen grains.

Pollen grains

Fig. A mature dihisced anther with pollen grains

Pollen grains (i) They develop from PMC due to meiotic division. (ii) They represent the male gametophyte. (iii) Pollen grains are generally spherical in outline. (iv) They possess two prominent layered walls.

152 (v) The outer layer is called exine and the inner layer is called intine.

Xam idea BiologyXII

(vi) The exine is the outermost hard layer chemically composed of sporopollenin which is one of the most resistant organic material. (vii) The exine has prominent apertures formed due to absence of sporopollenin through which the pollen tube develops is called germ pore. (viii) The innermost thin layer chemically composed of cellulose and pectin is called intine. (ix) The newly differentiated pollen grain has central nucleus and dense cytoplasm. (x) Vacuoles develop thereby pushing the nucleus towards the periphery. (xi) The protoplast thus divides mitotically forming two unequal cellsbigger vegetative which is rich in food reserve and smaller generative cell with dense cytoplasm and a nucleus.

(a)

Vacuoles Nucleus

Asymmetric spindle

Vegetative cell Generative cell (b)

(a) Enlarged view of a pollen grain tetrad (b) Stages of a microspore maturing into a pollen grain

Examination Papers OR (a)

At puberty Spermatogonia Mitosis differentiation CHROMOSOMES NUMBER PER CELL 46

153

Primary spermatocytes 1st meiotic division

Secondary spermatocytes 2nd meiotic division Spermatids Differentiation Spermatozoa

23

23

Fig. Schematic representation of spermatogenesis

(b) Structure of a sperm (Spermatozoa) (i) It consists of four parts: head, neck, middle piece and tail. (ii) Head: It is the enlarged end of a sperm, containing the large haploid nucleus, i.e., condensed chromatin body and is capped by acrosome, the acrosome contains hydrolytic enzymes that help in dissolving membranes of the ovum for fertilisation. (iii) Neck: It contains proximal centriole towards the nucleus which is necessary for the first cleavage division of zygote and the distal centriole that is connected to the tail filament. (iv) Middle piece: It contains a number of mitochondria that provide energy for the movement of the tail that facilitate sperm motility essential for fertilisation.
Head

Plasma membrane Acrosome Nucleus containing chromosomal material

Neck Middle piece Mitochondria (energy source for swimming)

Tail

Structure of a sperm

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Xam idea BiologyXII (v) Tail: It consists of an axial filament surrounded by the plasma membrane. It helps the sperms to swim in a fluid medium. (vi) A human male ejaculates about 200-300 million sperms during a coitus. Seminal plasma along with the sperms constitute the semen.

29. (a) Law of Independent Assortment According to this law the two factors of each character assort or separate out independent of the factors of other characters at the time of gamete formation and get randomly rearranged in the offsprings producing both parental and new combinations of characters. (b) Parents Genotype Gametes Tall and round seeds TTRR TR Dwarf and wrinkled seeds ttrr tr

F1 generation

TtRr On selfing
TtRr X TtRr

(All tall and round seeds)

TR Tr

tR

tr

TR Tr

tR

tr

TR F2 generation TR Tr tR tr TTRR Tall round TTRr Tall round TtRR Tall round TtRr Tall round

Tr TTRr Tall round TTrr Tall wrinkled TtRr Tall round Ttrr Tall wrinkled

tR TtRR Tall round TtRr Tall round ttRR Dwarf round ttRr Dwarf round

tr TtRr Tall round Ttrr Tall wrinkled ttRr Dwarf round ttrr Dwarf wrinkled

Tall round : Tall wrinkled : Dwarf round : Dwarf wrinkled 9 : 3 : 3 : 1 OR Hershey and Chase conducted their experiment on bacteriophage and proved that DNA is the genetic material. (i) They grew some bacteriophage virus on a medium that contained radioactive phosphorus (P 32 ) and some in another medium with radioactive sulphur (S 35 ) respectively. (ii) Viruses grown in the presence of radioactive phosphorus (P 32 ) contained radioactive DNA.

Dihybrid phenotypic ratio

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(iii) Similar viruses grown in presence of radioactive sulphur (S 35 ) contained radioactive protein. (iv) Both the radioactive viruses was allowed to infect E. coli separately. (v) Soon after infection the bacterial cells were gently agitated in blender to remove viral coats from the bacteria. (vi) The culture was also centrifuged to separate the viral particle from the bacterial cell. (vii) It was observed that only radioactive P 32 was found associated with the bacterial cell and S 35 was only in the surrounding medium and not in the bacterial cell. (viii) The result clearly indicates that only DNA and not protein coat entered the bacterial cell and this proves that DNA is the genetic material that is passed from virus to bacteria and not protein.
Bacteriophage Radioactive (35 S) labelled protein capsule Radioactive (32 P) labelled DNA

1. Infection

2. Blending

3. Centrifugation No Radioactive (35 S) detected in cells + Radioactive (35 S) detected in supernatant Radioactive (32 P) detected in cells + No Radioactivity detected in supernatant

The Hershey-Chase experiment

30. (a) Herbivores are animals feeding on plants. Although they are classed differently they are considered predators. Like predators transfer energy across trophic levels, herbivores also do

156

Xam idea BiologyXII the same. Besides this, they also keep the population of their prey under control. For e.g; when the prickly pear cactus was introduced in Australia in early 1920s, they spread rapidly causing havoc. Their population was controlled by introducing cactus-feeding predator (a moth). (b) (i) S.No. (i) (ii) (iii) Mutualism Competition This interaction benefits both the In this interaction both, the interacting species suffer negatively. interacting species. The two individuals may be physically There is no physical or physiologically associated. between the competitors. association

eg. Lichens represent mutualism eg. In some American Lakes, visiting between fungus and algae where flamingoes and resident species fungus absorbs nutrition and provides compete for their common food. protection, and algae prepares food.

(ii) Commensalism is the interaction between two species where one species is benefitted and the other is neither harmed nor benefitted, e.g., an orchid growing as an epiphyte on a mango tree benefits by getting shelter and nutrition but the mango tree is not affected. Ammensalism is the interaction between two different species, in which one species is harmed and the other is neither benefitted nor harmed. E.g, penicillium produces a toxin killing other microorganisms but is not affected itself. OR (a) The species that invade a bare area are called pioneer species, e.g., lichens. The lichen secretes some acids to dissolve rock and help in weathering and soil formation. Later, some small bryophytes invade and hold the small amount of soil. The bryophytes are succeeded by herbs, shrubs, and ultimately big trees. At last a stable climax forest is formed. The xerophytic habitat get converted into mesophytic one.
Lichens Mosses Annual grasses

Bare area A

Lichen-mosses Perennial B Grasses

C Shrubs

D Trees

Biotic succession on a bare rock

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(b) S.No. (i) (ii) (iii) Phosphorus cycle Phosphorus returns to the soil. Carbon cycle Carbon is atmosphere. released into the

The atmospheric input of phosphorus The atmospheric input of carbon through rainfall is much smaller. from rainfall is greater. There is no gaseous exchange of Carbon gas is exchanged between phosphorus between organism and organism and atmosphere during environment. respiration.

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SETII
SECTIONA 2. (i) Hb Hb
A S

(ii) Hb S Hb S 6. Zoospores are microscopic motile structures. These are special structures by which algae reproduce asexually. SECTIONB 9. Placenta produces several hormones like human chorionic gonadotropin (hCG), human placental lactogen (hPL), estrogens, progesterons that are essential to maintain pregnancy. This way placenta acts as an endocrine tissue. 10. Menarche is the beginning of menstruation at puberty. It starts at the age of 13-15. Menopause is the cessation of menstruation. It happens around 50 years of age. 13. Features of a stable community are as follows: (i) Communities should have greater biodiversity for greater stability. (ii) It should be able to prevent invasion by alien species. (iii) It should be able to restore itself in a short period of time. (iv) Variations should be minimal in the community. 17. Irreparable computers and other electronic goods are known as electronic wastes (e-wastes). E-wastes are buried in landfills or incinerated. Recycling is the only solution for the treatment of e-wastes. SECTIONC 21. (i) In plants bearing bisexual flowers, the anthers are removed from the flower before they dehisce. This is called emasculation. The emasculated flowers are covered with a bag of butter paper to prevent contamination of stigma with unwanted pollen. This process is called bagging. When this stigma attains receptivity, mature pollen grains are dusted on the stigma and the flowers are rebagged to allow the fruits to develop.

(ii) If the female parent produces unisexual flowers, emasculation is not done. The flower buds are bagged before the flowers open. When the stigma becomes receptive, pollen is dusted on stigma and the flower is rebagged. 27. In humans XY type of sex determination is seen. Both males and females have equal number of chromosomes. Males have one X-chromosome and a smaller Y-chromosome. While, females have a pair of X-chromosomes. Hence males have autosomes plus XY, while females have autosomes plus XX. This shows male heterogamety. In birds, female heterogamety is observed. They exhibit ZW type of sex determination. Both males and females have equal number of chromosomes. Female birds has one Z and one W chromosomes whereas males have a pair of Z-chromosomes beside the autosomes.

Examination Papers SECTIOND 30. (a) Megagametogenesis: The formation of female gametophyte (embryo sac) is called megagametogenesis. (i) Megaspore is the first cell of the female gametophyte.

159

(ii) The megaspore increase in size and its nucleus divides mitotically into two nuclei which move apart to opposite poles. Thus a 2-nucleate embryo sac is formed. (iii) Two daughter nuclei undergo another mitotic division giving rise to the 4-nucleate stage. (iv) The third mitotic division, which gives 8-nucleate embryo sac comprises of a micropylar and chalazal end with 4 nuclei at each end. (v) One nuclei from each end proceeds towards the centre of the embryo sac and are called the polar nuclei. (vi) The three nuclei remaining at the micropylar region form the egg apparatus, without any cellular wall. (vii) In the egg apparatus, the middle one is the largest called oosphere/egg/ovum, while other two naked cells adjoining the egg cell are called synergids. (viii) The three remaining nuclei at the chalazal end are surrounded by mass of cytoplasm and cellular wall and are called antipodal cells. (ix) The above mentioned method of female gametophyte formation is known as normal 8-nucleate type, because 8 nuclei contribute in the formation of gametophyte and it is very common among angiosperms. (b)
Plumule

Cotyledons Hypocotyl

Radicle Root cap

A diagrammatic representation of the mature dicot embryo

160 OR (a)

Xam idea BiologyXII

Spermatozoa Spermatid Secondary spermatocyte Primary spermatocyte Sertoli cell

Spermatogonium

Diagrammatic sectional view of a seminiferous tubule (enlarged)

(b) Spermatogenesis (i) The male germ cells of seminiferous tubules divide mitotically to produce diploid spermatogonia. (ii) Spermatogonia grow and increase in size and forms primary spermatocyte (2n). (iii) The primary spermatocytes undergo the first meiotic division and produces two haploid secondary spermatocytes (n). (iv) Each secondary spermatocyte undergoes the second meiotic division and produces four-equal haploid spermatids. (v) The spermatids are transformed into spermatozoa (sperms) by the process of spermiogenesis. (vi) A sperms head gets attached to Sertoli cells to draw nourishment and finally released from the seminiferous tubules by the process called spermiation.

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SETIII
SECTIONA 1 Due to non-disjunction, 21 pair of chromosomes fail to separate during oogenesis. Therefore, the egg possesses 24 chromosomes instead of 23. When the egg fuses with a sperm, the zygote will have three copies of chromosome 21 causing trisomy. 4. Pea flowers are cleistogamous, i.e., anther and stigma lie close to each other in closed flowers. So when anthers dehisce in the flower buds, pollen grains come in contact with the stigma to effect pollination. Thus, assured seeds are produced in pea. SECTIONB 13. S.No. (i) (ii) Follicular Phase Luteal Phase
st

During this, primary follicles grow to During this, remaining parts of Graafian become fully mature Graafian follicle. follicle transform as the corpus luteum. Endometrium proliferation. regenerates through Endometrium further thickens secreting progesterone for implantation during fertilisation. If fertilisation does not occur, corpus luteum degenerates.

15. Alien species turn invasive and cause decline or extinction of indigenous species. For example, the Nile perch introduced into Lake Victoria in east Africa led to the extinction of more than 200 species of cichlid fish in the lake. Invasive weed species like carrot grass (Parthenium) are very harmful to crop plants. 17. aHigh BOD due to sewage discharge. bIncrease in dissolved oxygen due to sewage decomposition. Micro-organisms involved in biodegradation of organic matter consume a lot of oxygen, therefore, there is a sharp decline in dissolved oxygen. When the sewage is completely degraded, oxygen concentration again increases. 18. Zygote intra fallopian transfer technique (ZIFT) is a technique in in vitro fertilisation where the zygote or early embryo having up to 8 blastomeres is transferred into the fallopian tube to complete its further development. Intra Uterine Transfer technique (IUT) is different from ZIFT as the embryos with more than 8 blastomeres are transferred into the uterus in IUT. SECTIONC 20. In grasshopper, the mechanism of sex determination is of the XO type. In females, the eggs bear a pair of X chromosomes along with the autosomes. On the other hand, there are two types of sperms formed in malesone having a X chromosome and other having no chromosome. Hence, grasshopper shows male heterogamety.

162 Male XO + AA X + A XX+AA Female O + A Female XX + AA X + A X + A XO + AA Male

Xam idea BiologyXII

Parents Gametes Progeny

In Drosophila, the mechanism of sex determination is of XY type. Females have a pair of X chromosomes, while the males have two types of spermsone having X chromosome and other having Y chromosome. XX XY type: Male AA + XY A + X A + Y Female AA + XX A + X

AA + XX AA + XY Female Male 27. (a) Contrivances or devices for self-pollination (Autogamy) (i) Cleistogamous flowers: These are bisexual closed flower which never open at all and the anthers dehisce inside these closed flowers, e.g., Commelina. (ii) Homogamy: It is the condition of the maturity of anther and stigma at the same time, e.g., Catharanthus (Vinca). (iii) The close association between anther and stigma, e.g., Mirabilis. (b) Geitonogamy: Transfer of pollen grains from the anther to the stigma of another flower of the same plant. Although geitonogamy is functionally cross-pollination involving a pollinating agent, genetically it is similar to autogamy, since the pollen grains come from the same plant. SECTIOND 29. (a) Structure of some Albuminous seeds
Seed coat Cotyledon Endosperm Shoot apical meristem Hypocotyl root axis Root tip Endosperm Onion Coleorrhiza V.S. of maize grain Scutellum Pericarp

Coleoptile Plumule Radicle

Examination Papers (b) Advantages of seeds: (i) Seeds posses better adaptive strategies for dispersal to form a new colony.

163

(ii) The reserve food of seed, support the growth of seedling till they become nutritionally independent. (iii) The hard seed coat (testa + tegmen) provide protection against injury. (iv) They provide genetic recombination and variation as they are the product of sexual reproduction. (v) Seeds are stored to be consumed throughout the year to overcome drought and natural calamities. (vi) Depending upon the mobility of seeds, they are used to raise crop in favourable season. OR (i) Post-zygotic events upto implantation and placenta formation (ii) During coitus, semen is released by the penis into the vagina (insemination). (iii) The motile sperms swim rapidly, through the cervix, enter into the uterus and reach the ampullaryisthmic junction of the oviduct. (iv) A sperm comes in contact with the zona pellucida layer of the ovum and induces changes in the membrane to block the entry of additional sperms. (v) The enzymes of the acrosome of sperm help to dissolve zona pellucida and plasma membrane of the ovum and sperm head is allowed to enter into the cytoplasm of the ovum, i.e., secondary oocyte. (vi) Ultimately diploid zygote is produced by the fusion of a sperm and an ovum. Implantation (i) Zygote divides rapidly by mitotic division called cleavage and as a result 2, 4, 8, 16 daughter cells are produced which are termed as blastomeres. (ii) Embryo with 816 blastomeres is called a morula. (iii) The morula changes into a large mass of cells called blastocyst which passes further into the uterus. (iv) Blastomeres in the blastocyst are arranged into an outer layer called trophoblast and an inner group of cells attached to trophoblast called inner cell mass. (v) The trophoblast layer gets attached to the cells of the endometrium and the inner cell mass gives rise to the embryo. (vi) The cells of endometrium divide rapidly and cover the blastocyst. (vii) So, the blastocyst gets embedded in the endometrium of the uterus called implantation leading to pregnancy. Functions of Placenta (i) Embryo get nutrients, oxygen and removes CO 2 and waste materials through placenta. (ii) An umbilical cord connects placenta with the embryo that helps in the transport of substances to and from the embryo. (iii) Placenta also acts as an endocrine tissue and produces several hormones like human chorionic gonadotropin (hCG), human placental lactogen (hPL), estrogens, progesterons that are essential to maintain pregnancy.

EXAMINATION PAPERS 2010

CBSE (FOREIGN) SETI Time allowed: 3 hours] General Instructions: (i) All questions are compulsory. (ii) This question paper consists of four sections A, B, C and D. Section A contains 8 questions of one mark each, Section B is of 10 questions of two marks each, Section C is of 9 questions of three marks each and Section D is of 3 questions of five marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled. [Max. Marks: 70

SECTIONA 1. Name an organism where cell division is itself a mode of reproduction. 2. Why is tubectomy considered a contraceptive method? 3. Write the percentage of F2 homozygous and heterozygous populations in a typical monohybrid cross. 4. When does a species become founders to cause founder effect? 5. Name two green-house gases produced by anaerobic microbes. 6. What is the host called that produces a foreign gene product? What is this product called? 7. Why do predators avoid eating Monarch butterfly? How does the butterfly develop this protective feature? 8. Why is the use of unleaded petrol recommended for motor vehicles equipped with catalytic converters? SECTIONB 9. Why are papaya and date palm plants said to be dioecious whereas cucurbits and coconut palms monoecious, in spite of all of them bearing unisexual flowers? 10. What is amniocentesis? Why has the government imposed a statutory ban in spite of its importance in the medical field? 11. Why are the wings of butterfly and birds said to be analogous organs? Name the type of evolution of which the analogous organs are a result of.

Examination Papers 12. Identify a, b, c and d in the following table: Name of the human disease 1. Typhoid 2. Common cold 3. Pneumonia Name of the causal bacteria/virus Salmonella typhi b Streptococcus pneumoniae

165

Specific organ or its part affected a c d

13. A schematic representation of polymerase chain reaction (PCR) upto the extension stage is given below. Answer the questions that follow:

5' 3' Heat 5' 3' 5' 3' 'c' + Deoxynucleotides 5' 5' 3' 3' 3' 5' 3' 5'

3' ds DNA 'a' 5'

3' 'b'

5'

3'

5'

14. 15. 16.

17.

(i) Name the process a. (ii) Identify b (iii) Identify c and mention its importance in PCR. Why do the toxic insecticidal proteins secreted by Bacillus thuringiensis kill the insect and not the bacteria itself? How do plants benefit from having mycorrhizal symbiotic association? Explain the action of the restriction endonuclease EcoRI. OR How are the DNA fragments separated by gel electrophoresis visualised and separated for use in constructing recombinant DNA? Why are small animals rarely found in the polar regions? Explain.

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18. Write any two hypotheses put forth by ecologists explaining the existence of greater biodiversity in tropical regions than in temperate regions. SECTIONC 19. Study the sectional view of human testis showing seminiferous tubules given below. Answer the questions that follow.

(b)

(a) (c)

(d)

(i) Identify a, b and c. (ii) Write the functions of a and d. 20. Work out a cross between true breeding red and white flowered dog-flower plants (snapdragon) up to F2 progeny. Explain the results of F1 and F2 generations. 21. Draw a schematic representation of dinucleotide. Label the following: (i) The components of a nucleotide (ii) 5 end (iii) N-glycosidic linkage (iv) Phosphodiester linkage. 22. Anthropogenic action can hasten the evolution. Explain with the help of a suitable example. 23. (a) All human beings have cellular oncogenes but only a few suffer from cancer disease. Give reasons. (b) How is a malignant tumor different from a benign tumor? OR Describe how biogas is obtained from an activated sludge. 24. Explain the process by which a bacterial cell can be made competent. Why is it essential to make bacterial cells competent in recombinant DNA technology? 25. Mention the cause and effect of inbreeding depression in cattle. How can it be overcome? Explain.

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167

26. How are somaclones cultured from explants in in-vitro conditions? Why are somaclones so called? 27. Explain biomagnification. How does the biomagnification of DDT affect the population of fish-eating birds? SECTIOND 28. Explain double fertilisation and trace the post-fertilisation events in sequential order leading to seed formation in a typical dicotyledonous plant. OR Explain the process of fertilisation in human female, and trace the post-fertilisation events in a sequential order up to implantation of the embryo. 29. (a) Explain with the help of a graph the population growth curve when resources are (i) limiting and (ii) not limiting. (b) Nature has a carrying capacity for a species. Explain. OR (a) Draw the pyramids of biomass in a sea and in a forest. Explain giving reasons why the two pyramids are different. (b) Pyramid of energy is always upright. Explain. 30. ABO blood grouping in human population exhibits four possible phenotypes from six different genotypes. Explain different mechanisms of inheritance involved in exhibiting the possibility of four phenotypes and six genotypes. OR Where do transcription and translation occur in bacteria and eukaryotes respectively? Explain the complexities in transcription and translation in eukaryotes that are not seen in bacteria.

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SETII
(Uncommon Questions to Set I) SECTIONA 2. List any two ways of measuring the standing crop of a trophic level. 3. Banana is a true fruit but is also a parthenocarpic fruit. Give a reason. SECTIONB 11. Mention four adaptive features that help cacti survive in xeric environment. 12. Why should a bisexual flower be emasculated and bagged prior to artificial pollination? 13. Why is origin of replication (ori) required to facilitate cloning into a vector? 14. Why is Saheli a well accepted contraceptive pill? SECTIONC 19. Explain adaptive radiation and convergent evolution by taking example of some of Australian marsupials and Australian placental mammals. 27. An organic farmer relies on natural predation for controlling plant pests and diseases. Justify giving reasons why this is considered to be a holistic approach. SECTIOND 28. Study the schematic representation of the genes involved in the lac operon given below and answer the questions that follow: p i p o z y a

(i) Identify and name the regulatory gene in this operon. Explain its role in switching off the operon. (ii) Why is lac operons regulation referred to as negative regulation? (iii) Name the inducer molecule and the products of the genes z and y of the operon. Write the functions of these gene products. OR (i) Describe the role of RNA polymerases in transcription in bacteria and in eukaryotes. (ii) Name the scientist who postulated the role of an 'adapter' in protein synthesis. Name the adapter molecule.

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SETIII
(Uncommon Questions to Set I and II) SECTIONA 2. In yeast and Amoeba the parent cell divides to give rise to two new individual cells. How does the cell division differ in these two organisms? 3. Differentiate between standing state and standing crop in an ecosystem. SECTIONB 9. Explain giving two reasons why pollen grains can be best preserved as fossils. 13. Mention the fate of Corpus Luteum and its effect on the uterus in absence of fertilisation of the ovum in a human female. 16. Name the first transgenic cow developed and explain the improvement in the quality of the product produced by it. 18. How do seals adapt to their natural habitat? Explain. SECTIONC 22. In England, during the post-industrialized period, the count of melanic moths increased in urban areas but remained low in rural areas. Explain. 24. Spermatogenesis in human males is a hormone regulated process. Justify. SECTIOND 29. Study the schematic representation of the genes involved in the lac operon given below and answer the questions that follow: p i p o z y a

(i) Identify and name the structural genes in this operon and trace the events that lead to the transcription of these structural genes. (ii) Why is lac operons regulation referred to as negative regulation? OR (i) DNA polymorphism is the basis of DNA finger printing technique. Explain. (ii) Mention the causes of DNA polymorphism.

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Xam idea BiologyXII

SOLUTIONS
SETI SECTIONA 1. Amoeba. 2. Tubectomy involves cutting of the Fallopian tube and tying of their ends. This way, the sperms are not able to reach the egg acting as a contraceptive method. 3. The ratio of a typical monohybrid cross is 1: 2: 1 where 50% are homozygous and 50% are heterozygous populations. 4. When the change in the allele frequency is so different in the new sample of population, they become a different species. The original drifted population becomes founder. 5. Carbon-dioxide and methane. 6. The host that produces a foreign gene product is called competent host. The product is called recombinant protein. 7. The Monarch butterfly is highly distasteful to its predator (birds) because of a special chemical present in its body. It acquires this chemical during its caterpillar stage by feeding on a poisonous weed. 8. Motor vehicles equipped with catalytic converter should use unleaded petrol because lead in the petrol inactivates the catalyst. SECTIONB (i) Papaya and date palm plants are dioecious, because male and female flowers are present on different plants, that is each plant is either male or female. (ii) Cucurbits and coconut palms are monoecious, because male and female flowers are present on the same plant. 10. Amniocentesis is a foetal sex determination test based on the chromosomal pattern in the amniotic fluid surrounding the developing embryo. Amniocentesis is used for sex determination which most people get done to kill female foetus. Therefore, it has been banned. 11. Wings of butterfly and birds are not anatomically similar structures though they perform similar functions. Hence, they are called analogous structures. Analogous organs result from convergent evolution. 12. (a) small intestine (c) nose and respiratory passage 13. (i) a : Denaturation process (ii) b : Primers (iii) c : Taq DNA polymerase. Taq polymerase is a thermostable enzyme which remains active during the high temperature induced denaturation of DNA. 14. The Bt toxin protein exists as inactive prototoxins but once an insect ingests the inactive toxin, it is converted into an active form of toxin due to the alkaline pH of the gut which solubilise the crystals. Therefore, it does not kill the bacteria. (b) rhino virus (d) alveoli of lungs. 9.

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15. Mycorrhizal association is found between fungi and the roots of higher plants. The fungi help the plant in the absorption of essential nutrients from the soil while plant in turn provides energy-yielding carbohydrates to fungi. 16. (i) The recognition sequence shows palindrome character in which the sequence of base pairs read the same on both the DNA strands, i.e., same in 5 3 or 3 5 directions, e.g., 5 G A A T T C 3 3 C T T A A G 5 (ii) The restriction endonuclease acts on specified length of a DNA and binds to the DNA at the recognition sequence. (iii) It cuts the opposite double helix of DNA having sugar-phosphate backbones; at little away from the centre of the palindrome sites. (iv) There are overhanging stretches called sticky ends on each strand, because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. OR Separation and isolation of DNA fragments: (i) The cutting of DNA by restriction endonucleases results in the short fragments of DNA, which can be separated by a technique known as gel electrophoresis. (ii) The DNA fragments are negatively charged and they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. (iii) Commonly used matrix is agarose, which is a natural linear polymer of D-galactose and 3, 6-anhydro-L-galactose which is extracted from sea weeds. (iv) The DNA fragments separate (resolve) out according to their size through sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it will move. (v) The separated DNA fragments can be visualised after staining the DNA with ethidium bromide followed by exposure to UV radiation. (vi) The separated bands of DNA are cut out and extracted from the gel piece, this step is called elution. (viii) The purified DNA fragments are used to form recombinant DNA which can be joined with cloning vectors.
Wells Largest
3

DNA bands

Smallest

A typical agarose gel electrophoresis showing migration of undigested (lane 1) and digested set of DNA fragments (lane 2 to 4)

17. In the cold regions like poles, the larger surface area the animal has, greater are its chances to survive. Since small animals have a small surface area they are not able to produce optimum body heat for their survival. Hence, they are rarely found in such regions.

172 18.

Xam idea BiologyXII (i) Tropical environments are less seasonal, relatively more constant and predictable than temperate environments. This promotes niche specialisation and results in greater diversity. (ii) There is more solar energy available in tropics which contributes to higher productivity and in turn greater biodiversity. SECTIONC

19.

(i) a : Spermatogonia (ii)

b : Interstitial cells

c : Spermatozoa d : Sertoli cells a : The spermatogonia or male germ cells undergo meiotic divisions leading to sperm formation. d : Sertoli cells provide nutrition to the germ cells. Red flower RR R Rr RR R r R F2 generation R r RR Red Rr Pink R r Rr Pink rr White Rr r White flower rr r (All pink colour flowers)

20. Parents Gametes F1 generation On selfing Gametes

Red : Pink 1 : 2 Genotypic ratio: RR : Rr 1 : 2 Comment: This is a case of Mendelian dominance as red and white both are not 21.

Phenotypic ratio:

: White : 1 : rr : 1 deviation and inheritance that shows incomplete expressed and produce pink trait in F1 .

Phosphodiester linkage H 5' end P C H A Nitrogenous base Pantose suger P H C H G Base Ribose sugar OH 3' end

N-glycosidic linkage

The components of a nucleotide

A dinucleotide

Nucleotide = Ribose sugar + Base + phosphate group.

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22. Anthropogenic Action (Due to human action): Excess use of herbicides, pesticides, etc. has only resulted in selection of resistant variety in a much lesser time scale which is equally true for microbes against which we employ antibiotics or drugs against eukaryotic organisms/cells. As a result of which resistant organisms/cells are appearing in a time scale of months or years and not centuries. For example, when the DDT was used for the first time, maximum mosquitoes died but few survived due to variation in a population. These mosquitoes show resistance to DDT and survived to reproduce successfully in the presence of DDT and gradually such mosquito population become DDT resistance just like natural selection. 23. (a) All humans have cellular oncogenes or proto oncogenes, but only a few suffer from cancer because cancer only occurs on activation of oncogenes. This activation is induced by carcinogens which can be physical, chemical or biological. The chemical carcinogens present in tobacco smoke have been identified as a major cause of lung cancer. (b) Types of Tumors Benign tumor (i) It is a non-cancerous tumor. (ii) Benign tumor is non-invasive. Malignant tumor It is a cancerous tumor. It shows metastasis by invading other parts of the body.

(iii) It stops growth after reaching a certain Malignant tumor shows indefinite growth. size. (iv) Limited adherence occurs amongst cells of There is no adherence amongst cells. benign tumor. They tend to slip past one another. (v) It is less fatal to the body. OR In activated sludge system, the primary effluent is taken to aeration tanks, where it is constantly agitated mechanically. Air is also pumped into it. A large number of aerobic heterotrophic microbes grow in the aeration tank. The microbes digest a lot of organic matter, converting it into microbial biomass and releasing lot of minerals. Methanogenic bacteria produce a mixture of gases containing methane, Hydrogen Sulphide and Carbon dioxide. The mixture called biogas is inflammable and is a source of energy.
Gas

It is more fatal to the body.

Gas-holder Dung Water [CH4 + CO2 +...] Sludge

Digester

A typical biogas plant

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Xam idea BiologyXII

24. Competent Host (For Transformation with Recombinant DNA) (i) DNA being a hydrophilic molecule, cannot pass through cell membranes. (ii) The bacteria should be made competent first, to accept the DNA molecules by treating them with a specific concentration of a divalent cation like calcium which increases the efficiency with which DNA enters the cell through the pores in its cell wall. (iii) Recombinant DNA can be forced into such cells by incubating the cells on ice, followed by placing them briefly at 42C (heat shock) and then putting them back on ice. This facilitates the entry of recombinant DNA in the bacterial cell. 25. Strategy for breeding (i) Superior males and superior females of the same breed are selected for mating to obtain the progeny. (ii) Among the progeny, superior males and females are again identified for mating to obtain a further good variety. (iii) One major advantage of inbreeding is that it increases the homozygosity. (iv) Inbreeding is necessary to develop a pure line in an animal. (v) Inbreeding exposes harmful recessive genes that are eliminated by selection. (vi) Inbreeding facilitates the accumulation of superior genes and elimination of the less desirable genes as per the requirement of breeding procedure. (vii) Sometimes close inbreeding reduces fertility and even productivity which is called inbreeding depression (viii) Outbreeding, it refers to the breeding of unrelated animals either of the same breeds (but do not have common ancestors) or of between different breeds (cross-breeding) or even different species (interspecific-hybridisation). 26. Tissue Culture (i) Tissue culture is an in vitro technique of regeneration of a whole plant from any part of a plant by growing it on culture medium under aseptic conditions. (ii) The capacity of a cell to grow or generate the whole plant is called totipotency. (iii) The part of the plant taken for tissue culture is called explant. (iv) The explant is grown in aseptic condition in synthetic/cultural media which is rich in inorganic nutrient, vitamins, amino acids and growth regulators like cytokinin and auxin. (v) The method of growing or producing thousands of plants through tissue culture is called micropropagation. (vi) Since plants produced from tissue culture are genetically identical to the original plant from which they are grown, so called somaclones. 27. Biological magnification: Unknowingly, some harmful chemicals enter our bodies through the food chain. We use several pesticides and other chemicals to protect our crops from diseases and pests. These chemicals are either washed down into the soil or into the water bodies. From the soil, these are absorbed by the plants along with water and minerals, and from the water bodies these are taken up by aquatic plants and animals. This is one of the ways in which they enter the food chain. As these chemicals are not degradable, these get accumulated progressively at each trophic level. As human beings occupy the top level in any food chain, the maximum concentration of these chemicals get accumulated in our bodies. This phenomenon is known as biological magnification.

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Biomagnification is the increase in concentration of toxic materials like heavy metals and pesticides at each trophic level of a food chain due to their accumulation in fat. When DDT was used to control mosquitoes in a lake of USA, 800 times more DDT was found in the phytoplanktons than in the water of the lake. Zooplanktons had about 13 times more DDT than phytoplanktons. It was also observed that the fish population had 940 times more DDT than zooplanktons and fish eating birds had 25 times more DDT than fish. SECTIOND 28. Double Fertilisation/Triple Fusion (i) After a positive pollen-pistil interaction, the pollen tube develops and enters the ovule through synergid guided by filiform apparatus. (ii) The pollen tube releases the two male gametes into cytoplasm of synergid. (iii) One of the male gametes moves towards egg and fuses with its nucleus to form a diploid cell called zygote. This is an event called syngamy. (iv) Another male gamete moves towards the polar nuclei in the centre, fuses to produce a triploid primary endosperm nucleus (PEN). (v) As this involves the fusion of three haploid nucleus (one male gamete and 2 polar nuclei) it is termed as triple fusion. (vi) As syngamy and triple fusion take place simultaneously in the embryo sac it is termed as double fertilisation. (vii) The central cell formed by triple fusion called primary endosperm cell (PEC) later develops into endosperm. (viii) The zygote later develops into an embryo.
Degenerating synergids Zygote (2n) Primary endosperm cell (PEC) Primary endosperm nucleus (3n) (PEN) Suspensor Radicle Zygote Cotyledon Degenerating antipodal cells (a) Heart-shaped embryo Plumule Mature embryo Globular embryo (b)

(a) Fertilised embryo sac showing zygote and Primary Endosperm Nucleus (PEN); (b) Stages in embryo development in a dicot [shown in reduced size as compared to (a)]

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Xam idea BiologyXII Post-Fertilisation Events 1. After double fertilisation, summation of all the events in a flower is called post-fertilisation events. 2. Post-fertilisation events include: (i) Development of endosperm from primary endosperm nucleus (PEN). (ii) Development of embryo from zygote. (iii) Development of seeds from ovule. (i) Development of Endosperm (a) Endosperm develops from PEN (primary endosperm nucleus). (b) Endosperm develops first-followed by an embryo. (c) The PEN undergoes successive nuclear divisions to give rise to free nuclei and this stage endosperm development is called free-nuclear endosperm. (d) This division is followed by cytokinesis and thus endosperm becomes cellular called cellular endosperm. (ii) Development of embryo Embryo develops from fertilised ovule. It is formed from zygote. The early stages of embryo development from a zygote comes under embryogeny. The formation of embryo starts only after a certain amount of endosperm formation to assure the nutrition supply for development and growth of embryo. (iii) Development of seeds (a) The zygote undergoes transverse division forming a large basal cell and a small apical or terminal cell. (b) The large basal cell enlarges and undergoes transverse division to from a group of 6-10 cells called suspensor. The first cell of the suspensor towards the micropylar end is called haustorium. While the last cell of the suspensor towards the chalazal end is called hypophysis that later develops into radicle. (c) The smaller terminal or apical cell undergoes one vertical division. The two cells formed from terminal cell divide by a transverse division thus farming four embryonal cell (quadrant stage). Finally these four cells divide vertically forming 8-celled proembryo (octate stage). Four cells at the apex give rise to plumule and another four give rise to hypocotyl except its tip. As a result of double fertilisation number of changes takes place in an ovule due to which ovule is converted into seeds. OR Fertilisation (i) The process of fusion of a sperm (male gamete) with an ovum (female gamete) is called fertilisation. (ii) During coitus, semen is released by the penis into the vagina (insemination). (iii) The motile sperms swim rapidly, through the cervix, enter into the uterus and reach the ampullaryisthmic junction of the oviduct. (iv) A sperm comes in contact with the zona pellucida layer of the ovum and induces changes in the membrane to block the entry of additional sperms.

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(v) The enzymes of the acrosome of sperm help to dissolve zona pellucida and plasma membrane of the ovum and sperm head is allowed to enter into the cytoplasm of the ovum, i.e., secondary oocyte. (vi) Ultimately diploid zygote is produced by the fusion of a sperm and an ovum. Implantation (i) Zygote divides rapidly by mitotic division called cleavage and as a result 2, 4, 8, 16 daughter cells are produced which are termed as blastomeres. (ii) Embryo with 816 blastomeres is called a morula. (iii) The morula changes into a large mass of cells called blastocyst which passes further into the uterus. (iv) Blastomeres in the blastocyst are arranged into an outer layer called trophoblast and an inner group of cells attached to trophoblast called inner cell mass. (v) The trophoblast layer gets attached to the cells of the endometrium and the inner cell mass gives rise to the embryo. (vi) The cells of endometrium divide rapidly and cover the blastocyst. (vii) So, the blastocyst gets embedded in the endometrium of the uterus called implantation leading to pregnancy. 29. (a) Population Growth Models l There are two models of population growth: (a) logistic growth (b) The exponential growth (a) Logistic growth
l

When resources are limited logistic growth curve is followed. When N is plotted in relation to time t, the logistic growth show sigmoid curve and also called Verhulst-Pearl logistic Growth. K- N dN / dt = rN , K Where N = Population density at time t , r = Intrinsic rate of natural increase K = Carrying capacity.

(b) The exponential or geometric growth is common where the resources (food + space) are not limited. The equation becomes dN = (b - d) N, dt Let (b - d) = r, then dN = rN dt

where r is called intrinsic rate of a natural increase and it is a very important parameter chosen for observing impacts of any biotic or abiotic factors on population growth. In exponential growth, when N in relation to time is plotted on graph, the curve becomes J shaped and the equation becomes Nt = N 0e rt

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Xam idea BiologyXII Where Nt = population density after time t, N0 = population density at time zero. r = intrinsic rate of natural increase, e = the base of natural logarithms (2.71828).
dN = rN dt K b

Population density (N)

a dN = rN KN K dt

( )

Time (t)

Population growth curve (a) when responses are limiting the growth, plot is logistic, K is carrying capacity (b) when responses are not limiting the growth, plot is exponential.

(b) (i) The resources become limited at certain point of time, so no population can grow exponentially. (ii) Every ecosystem or environment or habitat has limited resources to support a particular maximum number of individuals called its carrying capacity (K). OR (a)
PC PP 4 21

Inverted pyramid of biomass in a sea ecosystem

Trophic level Dry weight (kg m2 )

TC

1.5

SC PC PP

11 37 809

Pyramid of biomass in a forest ecosystem

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The pyramid of biomass in sea is inverted because the biomass of fish far exceeds that of phytoplankton. The pyramid of biomass in a forest ecosystem is upright, because in the biomass. (b) Pyramid on energy is never inverted because when energy flows from a particular trophic level to the next trophic level, some energy is always lost as heat at each step. Each bar in the energy pyramid indicates the amount of energy present at each trophic level in a given time. 30. The human blood is responsible for gene I, and gene I exists in three alternative forms I A , I B , and i. I A and I B are dominant over i allele, and I A and I B are codominant for the production of RBC surface antigens A and B respectively. When the allele of blood group A (I A ) and blood group B (I B ) come together in an individual, blood group AB is produced and it depicts the phenomena of co-dominance. Table showing the genetic basis of blood groups in human population Allele from Parent 1 IA IA I
A

Allele from Parent 2 IA IB i IA I

B

Genotype of offspring I AI A I AI B I i I AI B I I
B B A

Blood types of offspring A AB A AB B B O

IB I
B

IB i

i i

I Bi ii

OR Transcription and translation in bacteria occurs in the cytoplasm of the cell. Whereas in eukaryotes, transcription occurs in the nucleus and translation occurs in the cytoplasm. Complexities in Transcription in Eukaryotes (i) The structural genes are monocistronic and split in eukaryotes. (ii) The genes of eukaryotic organisms having coding or expressed sequences are called exons that form the part of mRNA and non-coding sequence are called introns, that do not form part of the mRNA and are removed during RNA splicing. (iii) In eukaryotes, apart from the RNA polymerase found in the organelles, three types of RNA polymerases are found in the nucleus. (iv) RNA polymerase I transcribes rRNAs (28S, 18S, and 5.8S). (v) RNA polymerase II transcribes the precursor of mRNA (called as heterogeneous nuclear RNA (hnRNA). (vi) RNA polymerase III helps in transcription of tRNA, 5S rRNA, and snRNAs (small nuclear RNAs).

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Xam idea BiologyXII (vii) The primary transcripts contain both the coding region exon and non-coding region intron in RNA and are non-functional called hnRNA. (viii) The hnRNA undergoes two additional processes called capping and tailing. (ix) In capping, an unusual nucleotide is added to the 5-end of hnRNA methyl guanosine triphosphate. (x) In tailing, adenylate residues (about 200300) are added at 3-end in a template independent manner. (xi) Now the hnRNA undergoes a process where the introns are removed and exons are joined to form mRNA called splicing. Translation in both eukaryotes and prokaryotes is similar.

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SETII
SECTIONA 2. Standing crop is measured as the biomass or the number in a unit area. 3. Since banana develops only from the ovary it is a true fruit and since it develops without fertilisation it is parthenocarpic. SECTIONB 11. Adaptation in desert plants: (i) Desert plants have thick waxy coating on leaf called cuticle for minimum loss of water, through transpiration. (ii) They have special photosynthetic pathway (CAM) that enables minimum loss of water during day time because stomata remain closed. (iii) Some desert plants develop spines instead of leaf and photosynthetic function is carried out by the flattened stem. 12. A bisexual flower is emasculated to prevent self-pollination in the flower and it is bagged after emasculation to prevent contact of unwanted pollen grain with the stigma of the flower. 13. Ori is a sequence from where replication starts and any piece of DNA to replicate in the host cell needs to be linked to it. It also controls the copy number of the linked DNA. 14. Saheli is well accepted because it contains a non-steroidal preparation and has very few side effects. SECTIONC 19. The process of evolution of different species in a given geographical area starting from a point and literally radiating to other areas of geography (habitats) is called adaptive radiation. Australian marsupials are good examples. A number of marsupials, each different from the other, evolved from an ancestral stock, but all within the Australian island continent. When more than one adaptive radiation appeared to have occurred in an isolated geographical area convergent evolution is said to occur. Placental mammals in Australia also exhibit adaptive radiation in evolving into varieties of such placental mammals each of which appears similar to a corresponding marsupial. Eg., Placental wolf and Tasmanian wolf (marsupial). 27. Besides acting as conduits for energy transfer across trophic levels, predators are used in biological control of plant pests. This ability of the predator is based on its regulating the prey population. The natural predators reduce interspecific competition and does not harm the crop plants. For example, in an area the invasive cactus can be brought under control by cactus-feeding predator (a moth). Using natural predation, the ecosystem is kept stable without harming any of the trophic levels.

182 SECTIOND 28. (i) i gene is the regulatory gene and stands for inhibitor.

Xam idea BiologyXII

The repressor of the operon is synthesised from the i gene. The repressor protein in the absence of an inducer (lactose or allolactose) binds to the operator region of the operon and prevents RNA polymerase from transcribing the protein. Thus switching off the operon. (ii) Regulation by lac operon is referred to as negative regulation because the repressor binds to the operator for switching off the operon. (iii) Lactose or allolactose acts as an inducer. (a) Gene z codes for b-galactosidase (b-gal) enzyme which breaks lactose into galactose and glucose. (b) Gene y codes for permease, which increases the permeability of the cell to lactose. (c) Gene A codes for enzyme transacetylase, which catalyses the transacetylation of lactose in its active form. OR Role of Polymerase in Transcription in Bacteria (i) (a) Initiation: s (sigma) factor recognises the start signal and promotor region on DNA and the s (sigma) with RNA polymerase binds to the promoter and initiate transcription. (b) Elongation: The RNA polymerase after initiation of RNA transcription loses the s-factor but continues the process of RNA formation. (c) Termination: Once the RNA polymerase reaches the termination region of DNA, the RNA polymerase is separated from DNA-RNA hybrid, as a result nascent RNA separates. This process is called termination which is facilitated by certain termination factor r. (d) In prokaryotes, mRNA does not require any processing, so transcription and translation occurs both in the cytosol. It can be said that the transcription and translation are coupled together. Role of Polymerase Transcription in Eukaryotes (a) In eukaryotes, apart from the RNA polymerase found in the organelles, three types of RNA polymerases are found in the nucleus. (b) RNA polymerase II transcribes the precursor of mRNA (called as heterogeneous nuclear RNA (hnRNA). (c) RNA polymerase III helps in transcription of tRNA, 5S rRNA, and snRNAs (small nuclear RNAs). (ii) Francis Crick postulated the role of an adapter. tRNA or transfer RNA is the adapter molecule.

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SETIII
SECTIONA 2. In Amoeba, binary fission takes place whereas in yeast cell division occurs by budding. 3. In an ecosystem, standing crop is the mass of living material in each trophic level at a particular time. Whereas standing state refers to the amount of nutrients in the soil at any given time. 9. 13. 16. 18. SECTIONB (i) Pollens are produced in large numbers. (ii) The sporopollenin in exine protects the pollen from harsh conditions. In the absence of fertilisation, corpus luteum degenerates and this causes disintegration of the endometrium of ovary leading to menstruation. Rosie was the first transgenic cow. It produced human protein-enriched milk (2.4 gms per litre). Seals adapt to the cold climate by having a thick layer of fat (blubber) below their skin that acts as an insulator and to reduce excess loss of body heat.

SECTIONC 22. Industrial Evidence is a case of natural selection. In England, it was observed before industrialisation that white-winged moth were more than dark-winged moth. But the situation became reversed after industrialisation. It was found that predators will spot and pick a moth against a contrasting background. During pre-industrialisation, the tree trunks were covered by white lichen and on white-background dark coloured moth could be picked up. During post industrialisation, the tree trunks were covered by dark, dust, coal particles and became dark, on which white moth could easily be picked up. Thus, it was found that industrial melanism supports evolution by natural selection. 24. Hormonal control of spermatogenesis (i) The gonadotropin releasing hormone (GnRH) secreted by the hypothalamus initiates spermatogenesis at the age of puberty. (ii) The increased levels of GnRH stimulates the anterior pituitary which then secretes the FSH (follicle stimulating hormone) and LH (luteinising hormone). FSH acts on the Sertoli cells and stimulates secretion of some factors which help in spermiogenesis. (iii) LH acts on the Leydig cells and causes the secretion of testosterone from the testis. Flow chart showing the hormonal control of the human male reproductive system
Inhibits GnRH and LH Production LH LEYDIG CELLS TESTOSTERONE STIMULATES SPERMATOGENESIS TESTOSTERONE REPRODUCTIVE TRACT and OTHER ORGANS INHIBIN HYPOTHALAMUS GnRH ANTERIOR PITUITARY TESTIS FSH SERTOLI CELLS

184 SECTIOND 29.

Xam idea BiologyXII

(i) The three structural genes are z, y, q. The lac z, lac y, and be q genes are transcribed from a lac zyq transcription unit under the control of a single promoter Plac. They encode enzyme required for the use of lactose as a carbon source. (a) Lactose acts as an inducer which binds to the repressor and forms an inactive repressor. (b) The repressor fails to bind to the operator region. (c) The RNA polymerase binds to the operator and transcript lac mRNA. (ii) Regulation by lac operon is referred to as negative regulation because the repressor binds to the operator for switching off the operon. OR (i) Allelic sequence variation has traditionally been described as a DNA polymerism if more than frequency greater than 0.01. Simply, if an inheritable mutation is 1 observed in a population at high frequency, it is referred to as DNA polymorphism. The probability of such variation to be observed in non-coding DNA sequence would be higher as mutations in these sequences may not have any immediate effect in an individual's reproductive ability. These mutations keep on accumulating generation after generation, and form one of the basis of variablility. There is a variety of different types of polymorphisms ranging from single nucleotide change to very large scale changes. For evolution and speciation, such polymorphisms play very important role. The single nucleotide polymorphisms is used in locating diseases and tracing of human history. (ii) DNA polymorphisms is due to mutation.

CBSE Examination Papers (Delhi 2011)

Time: 3 Hours Max. Marks: 70 General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. SectionA contains 8 questions of 1 mark each. SectionB is of 10 questions of 2 marks each. SectionC is of 9 questions of 3 marks each and SectionD is of 3 questions of 5 marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.

SETI
SECTIONA 1. Name the type of cell division that takes place in the zygote of an organism exhibiting haplontic life cycle. 2. Write the scientific name of the microbe used for fermenting malted cereals and fruit juices. 3. Write the unit used for measuring ozone thickness. 4. Name the event during cell division cycle that results in the gain or loss of chromosome. 5. How can bacterial DNA be released from the bacterial cell for biotechnology experiments? 6. Write the importance of cryopreservation in conservation of biodiversity. 7. Mention the role of the codons AUG and UGA during protein synthesis. 8. Normally one embryo develops in one seed but when an orange seed is squeezed many embryos of different shapes and sizes are seen. Mention how it has happened. SECTIONB 9. How do histones acquire positive charge? 10. Why is CuT considered a good contraceptive device to space children? 11. Differentiate between albuminous and non-albuminous seeds, giving one example of each. 12. Explain the process of RNA interference. 13. List the key tools used in recombinant DNA technology. 14. Name the two types of immune systems in a human body. Why are cell mediated and humoral immunities so called?

186 Xam idea BiologyXII OR Write the scientific names of the causal organisms of elephantiasis and ringworm in humans. Mention the body parts affected by them. 15. Justify with the help of an example where a deliberate attempt by humans has led to the extinction of a particular species. 16. Identify A, D, E and F in the diagram of an antibody molecule given below:

17. Study the graph given below. Explain how is oxygen concentration affected in the river when sewage is discharged into it.

18. Explain how a hereditary disease can be corrected. Give an example of first successful attempt made towards correction of such diseases. SECTIONC 19. Draw a diagram of a male gametophyte of angiosperm. Label any four parts. Why is sporopollenin considered the most resistant organic material? 20. How are dominance, codominance and incomplete dominance patterns of inheritance different from each other? 21. The base sequence in one of the strands of DNA is TAGCATGAT. (i) Give the base sequence of its complementary strand.

Examination Papers 187 (ii) How are these base pairs held together in a DNA molecule? (iii) Explain the base complementarity rules. Name the scientist who framed this rule. 22. (a) Sickle celled anaemia in humans is a result of point mutation. Explain. (b) Write the genotypes of both the parents who have produced a sickle celled anaemic offspring. 23. What is inbreeding depression and how is it caused in organisms? Write any two advantages of inbreeding. 24. (a) Identify A and B illustrations in the following: (i)

(ii)

(b) Write the term given to A and C and why? (c) Expand PCR. Mention its importance in biotechnology. 25.

The diagram above is that of a typical biogas plant. Explain the sequence of events occurring in a biogas plant. Identify a, b and c. 26. How can crop varieties be made disease-resistant to overcome food crisis in India? Explain. Name one disease-resistant variety in India of: (a) Wheat to leaf and stripe rust. (b) Brassica to white rust.

188 Xam idea BiologyXII OR Write the source and the effect on the human body of the following drugs: (i) Morphine (ii) Cocaine (iii) Marijuana 27. Name the type of interaction seen in each of the following examples: (i) Ascaris worms living in the intestine of humans (ii) Wasp pollinating fig inflorescence (iii) Clown fish living among the tentacles of sea-anemone (iv) Mycorrhizae living on the roots of higher plants (v) Orchid growing on a branch of a mango tree (vi) Disappearance of smaller barnacles when Balanus dominated in the coast of Scotland. SECTIOND 28. (a) Draw a labelled diagram of the human female reproductive system. (b) Enumerate the events in the ovary of a human female during: (i) Follicular phase (ii) Luteal phase of menstrual cycle. OR (a) Write the specific location and the functions of the following cells in human males: (i) Leydig cells (ii) Sertoli cells (iii) Primary spermatocyte. (b) Explain the role of any two accessory glands in human male reproductive system. 29. Explain the salient features of Hugo de Vries theory of mutation. How is Darwins theory of natural selection different from it? Explain. OR (a) Name the primates that lived about 15 million years ago. List their characteristic features. (b) (i) Where was the first man-like animal found? (ii) Write the order in which Neanderthals, Homo habilis and Homo erectus appeared on earth. State the brain capacity of each one of them. (iii) When did modern Homo sapiens appear on this planet? 30. (a) Explain primary productivity and the factors that influence it. (b) Describe how do oxygen and chemical composition of detritus control decomposition. OR (a) What is El Nino effect? Explain how it accounts for biodiversity loss. (b) Explain any three measures that you as an individual would take, to reduce environmental pollution.

Examination Papers 189

SETII (Questions Uncommon to SETI)

SECTIONA 1. How does Penicillium reproduce asexually? 5. Mention the uses of cloning vector in biotechnology. 8. Mention the function of trophoblast in human embryo. SECTIONB 10. Name an oral pill used as a contraceptive by human females. Explain how does it prevent pregnancy. 14. Explain the advantage of cross-breeding of the two species of sugar cane in India. OR How do cellular barriers and cytokine barriers provide innate immunity in humans? SECTIONC 19. Differentiate between geitonogamy and xenogamy in plants. Which one between the two will lead to inbreeding depression and why? 22. A pea plant with purple flowers was crossed with white flowers producing 50 plants with only purple flowers. On selfing, these plants produced 482 plants with purple flowers and 162 with white flowers. What genetic mechanism accounts for these results? Explain. 23. Mention the property of plant cells that has helped them to grow into a new plant in in vitro conditions. Explain the advantages of micropropagation. SECTIOND 30.

(a) (i) Name the biogeochemical (nutrient) cycle shown above. (ii) Name an activity of the living organisms not depicted in the cycle by which this nutrient is returned to the atmosphere. (b) How would the flow of the nutrient in the cycle be affected due to large scale deforestation? Explain giving reasons. (c) Describe the effect of an increased level of this nutrient in the atmosphere on our environment. OR (a) Healthy ecosystems are the base of wide range of (ecosystem) services. Justify. (b) Explain the differences and the similarities between hydrarch and xerarch successions of plants.

190 Xam idea BiologyXII

SETII (Questions Uncommon to SETI & SETII)

SECTIONA 3. Why green plants are not found beyond a certain depth in the ocean? SECTIONB 9. State the dual role of deoxyribonucleoside triphosphates during DNA replication. 13. Explain the role of Ti plasmids in biotechnology. 16. State the functions of primary and secondary lymphoid organs in humans. SECTIONC 21.

Why do you see two different types of replicating strands in the given DNA replication fork? Explain. Name these strands. 25. (a) Why do farmers prefer biofertilisers to chemical fertilisers these days? Explain. (b) How do Anabaena and mycorrhiza act as biofertilisers? 26. (a) Name the stage of Plasmodium that gains entry into the human body. (b) Trace the stages of Plasmodium in the body of female Anopheles after its entry. (c) Explain the cause of periodic recurrence of chill and high fever during malarial attack in humans. OR Trace the events that occur in human body to cause immunodeficiency when HIV gains entry into the body. SECTIOND 28. (a) Describe the stages of oogenesis in human females. (b) Draw a labelled diagram of a human ovum released after ovulation. OR (a) When and where does spermatogenesis occur in a human male? (b) Draw a diagram of a mature human male gamete. Label the following parts: Acrosome, nucleus, middle piece and tail. (c) Mention the functions of acrosome and middle piece.

Examination Papers 191

SOLUTIONS
SETI
SECTIONA 1. Meiosis. 2. Saccharomyces cerevisiae. 3. Dobson unit. 4. Failure of segregation of chromosomes. 5. The bacterial cell wall is digested by the enzyme lysozyme to release DNA from the cell. 6. By cryopreservation, the reproductive parts of rare species can be preserved. 7. The codon AUG initiates protein synthesis whereas the codon UGA stops protein synthesis. 8. An orange seed has many embryos because of polyembryony.

SECTIONB 9. Histones are rich in the basic amino acid residues lysines and arginines, which carry positive charges in their side chains. Therefore, histones are positively charged. 10. CuT increases phagocytosis of sperms within the uterus and suppresses sperm motility and its fertilising capacity. Thus, it a very effective method. 11. Albumious seeds have residual endosperm in them. For example, maize. Non-albuminous seeds do not have any residual endosperm. For example, pea. 12. RNA interference takes place in all eukaryotic organisms as a method of cellular defence. It involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA. 13. The key tools used in recombinant DNA technology are: (i) Restriction enzymes (ii) Polymerase enzyme (iii) Ligase enzyme (iv) Vectors (v) Host organism/cell. 14. The two types of immune systems in a human body are innate and adaptive immunity. Humoral immunity is called so because it consists of antibodies that are present in humors or body fluids, whereas cell-mediated immunity is provided by B-cells and T-cells. OR
Disease Elephantiasis Ringworm Causal Organism Wuchereria bancrofti and Wuchereria malayi. Microsporum, Trichophyton and Epidermophytor Body Parts Affected Lymph vessel of lower limb and genital organs. Skin, nails and scalp.

15. When Nile perch, a large predator fish, was introduced in Lake Victoria, it started feeding on the native fish, chichlid fish. As a result, chichlid fish became extinct and Nile perch, not finding any food for itself, died too. 16. AAntigen binding site DLight chain EHeavy chain FDisulfide bridge.

192 Xam idea BiologyXII 17. When sewage is discharged into the river, the oxygen concentration declines sharply because a large amount of oxygen is consumed by aerobic microorganisms in river to decompose the organic matter in river. When the amount of organic matter reduces, the amount of dissolved oxygen again increases. 18. A hereditary disease can be corrected by gene therapy. In this method, genes are inserted into a persons cells and tissues to treat a disease. The first successful attempt was gene therapy for adenosine deaminase (ADA) deficiency. SECTIONC 19.

Sporopollenin is considered the most resistant organic material because it can withstand high temperatures and strong acids and alkalis. Also it cannot be degraded by any enzyme. 20. Dominance: It is a phenomenon in which when two contrasting alleles are present together, only one expresses itself and is called dominant whereas the other which does not express itself is called recessive. Codominance: It is a phenomenon in which when two contrasting alleles are present together, both of the alleles express themselves. Incomplete dominance: It is a phenomenon in which when two contrasting alleles are present together neither of the alleles is dominant over other and the phenotype formed is intermediate of the two alleles. 21. (i) The complementary strand is ATCGTACTA. (ii) The base pairs are held together by hydrogen bonds in a DNA molecule. A and T are held by two hydrogen bonds while G and C are held by three hydrogen bonds. (iii) Watson and Crick framed the base complementarity rule. The rule states that the ratios between adenine and thymine, and guanine and cytosine are constant and equals one. 22. (a) In sickle cell anaemia, due to point mutation there is a substitution of a single nitrogen base at the sixth codon of the -globin chain of haemoglobin. GAG
Mutation

GUG

Glutamic acid Valine

(b) The genotypes of both the parents would be HbA HbS and HBAHbS. 23. Continued inbreeding, especially close inbreeding, usually reduces fertility and even productivity. This is called inbreeding depression. Advantages of inbreeding: (i) It exposes harmful recessive genes that are eliminated by selection. (ii) It helps in developing a pureline in any animal. 24. (a) ARecognition site of the restriction endonuclease. BOrigin of replication

Examination Papers 193 (b) ACoding strand CTemplate strand. (c) PCR stands for polymerase chain reaction. PCR is used to amplify DNA segments to a large number within a short span of time. 25. The biogas plant tank is fed with slurry of dung. A floating cover is placed over the slurry which keeps on rising as the gas is produced in the tank due to the microbial activity of methanogens like Methanobacterium. Methanogens grow anaerobically on cellulosic material in cowdung to produce large amount of methane, CO2 and H2. The biogas plant has an outlet, which is connected to a pipe to supply biogas. The spent slurry is removed through another outlet and is used as fertiliser. aSludge tank, bGas holder, cCharge pit 26. Crop varieties can be made disease-resistant by conventional breeding methods or by mutation breeding. The germplasm is screened for resistance sources or mutations are introduced, followed by hybridisation of selected parents. The resulting hybrids are evaluated and tested. Finally, disease-resistant varieties are released. Disease-resistant variety of: (a) Wheat to leaf and stripe rustHimgiri. (b) Brassica to white rustPusa swarnim. OR (i) Morphine: It is obtained from poppy plant Papaver somniferum. It binds to specific opioid receptors present in central nervous system and gastrointestinal tract. (ii) Cocaine: It is obtained from coca plant Erythroxylum coca. It interferes with the transport of the neurotransmitter dopamine. (iii) Marijuana: It is obtained from cannabis sativa. It affects the cardiovascular system of the body. 27. (i) Parasitism (ii) Mutualism (iii) Commensalism (iv) Mutualism (v) Commensalism (vi) Competition SECTIOND 28. (a)

Diagrammatic sectional view of female pelvis showing reproductive system

194 Xam idea BiologyXII (b) (i) In the follicular phase, following events occur: 1.  The primary follicles in the ovary grow to form a fully mature Graafian follicle. 2. The endometrium of uterus regenerates through proliferation. 3. The secretion of gonadotropins (LH and FSH) gradually increases. (ii) In the luteal phase, following events occur: 1.  The parts of Graafian follicle remaining after the rupture, transform as the corpus luteum. 2. The corpus luteum secretes large amounts of progesterone. (a)
Cells (i) Leydig cells (ii) Sertoli cells (iii) Primary spermatocyte

OR
Location Interstitial space Seminiferous tubules Inner wall of seminiferous tubules Function Synthesis and secretion of testicular hormones called androgens. Provide nutrition to the germ cells. Undergo meiotic divisions to form secondary spermatocyte and then haploid sperms.

(b) The two accessory glands are: (i) Prostate gland: It surrounds the urethra and produces a milky secretion which forms a considerable part of semen. This secretion contains citric acid, lipids and enzymes. The secretion nourishes and activates the spermatozoa to swim. (ii) Seminal vesicles: These secrete mucous and a watery alkaline fluid that contains fructose which provides energy to the sperms. 29. Salient features of theory of Hugo de Vries: (i) Mutations cause evolution. (ii) New species originate due to large mutations. (iii) Evolution is a discontinuous process and not gradual. (iv) Mutations are directionless, (v) Mutations appear suddenly. (vi) Mutations exhibit their effect immediately.
Darwins Theory of Natural Selection 1.  He believed that minor variations cause evolution. 2.  Darwinian variations are small and directional. 3. He believed evolution to be gradual. Vries Theory of Mutation 1. He believed that mutation causes evolution. 2. Mutations are random and directionless. 3.  He believed sudden mutations caused evolution.

OR (a) Primates called Dryopithecus and Ramapithecus lived 15 million years ago. Their characteristic features are: (i) They were hairy and walked like gorillas and chimpanzees.

Examination Papers 195 (ii) Ramapithecus was more man-like. (iii) Dryopithecus was more ape-like. (b) (i) First man-like animal was found in Ethiopia and Tanzania. (ii) The order of appearance from the earliest to the latest is: Homo habilis, Homo erectus, Neanderthals. The brain capacity of Homo habilis is 650800 cc, of Homo erectus is 900 cc and of Neanderthals is 1400 cc. (iii) Modern Homo sapiens appeared between 75,00010,000 years ago. 30. (a) Primary productivity is defined as the amount of biomass or organic matter produced per unit area over a time period by plants during photosynthesis. It varies in different types of ecosystems. It is expressed in terms of weight (g2) or energy (kcal m2). It depends on the following factors: (i) Plant species inhabiting a particular area (ii) Environmental factors (iii) Availability of nutrients (iv) Photosynthetic capacity of plants. (b) Decomposition of detritus is slow if it contains lignin, chitin, tannins and cellulose, whereas it is quicker if detritus is made up of nitrogenous compounds and watersoluble substances like sugars. This is because the latter are easy to degrade. Oxygen is required for the activity of decomposers and detrivores. Therefore, a reduced oxygen amount will slow down the process of decomposition. OR (a) El Nino effect refers to the odd climatic changes due to rise in temperature. It leads to increased melting of polar ice caps as well as other places like the Himalayan snow caps. This will result in a rise in sea level that can submerge many coastal areas. Thus, resulting in biodiversity loss. (b) Measures to reduce environmental pollution (i) Use of renewable energy resources. (ii) Use of television and other instrument at low pitch. (iii) Minimum use of fossil fuel. (iv) No tobacco smoking. (v) Planting more trees.

SETII
SECTIONA 1. Pencillium reproduces asexually by spore formation. 5. Cloning vectors are used for transferring fragments of foreign DNA into a suitable host. They are also used to select recombinants from non-recombinants. 8. Trophoblast is the outer layer of blastocyt which helps the attachment of blastocyst to the endometrium of the uterus. SECTIONB 10. Saheli is an oral pill used as a contraceptive by females. Oral pills inhibit ovulation and implantation as well as alter the quality of cervical mucus to prevent or retard entry of sperms.

196 Xam idea BiologyXII 14. Saccharum barberi, grown in north India, had poor sugar content and yield, whereas Saccharum officinarum, grown in south India, had thicker stem and higher sugar content. The sugar cane species obtained after cross breeding these two species had thick stems, high sugar, high yield and ability to grow in north India. OR Cellular barriers include leucocytes (WBC) like polymorphonuclear leucocytes (neutrophils) and monocytes and natural killer cells in the blood as well as macrophages in tissues which phagocytose and destroy microbes. Cytokine barriers include interferons secreted by virus-infected cells, which protect noninfected cells from further viral infection. SECTIONC 19. Geitonogamy Xenogamy
1. It is transfer of pollen grains from the 1. It is transfer of pollen grains from the anther to the stigma of another flower of anther to the stigma of a different plant. same plant. 2. The pollen grains are genetically similar to 2. The pollen grains are genetically different the plant. from the plant.

Geitonogamy will lead to inbreeding depression because the pollen grains are genetically similar, which results in inbreeding. Continued inbreeding will thus reduce fertility and productivity. 22. The gene for purple flowers is dominant over that of white flowers. So, when two pure varieties are crossed, the F1 generation has only purple flowers and on selfing, the flowers are produced in a 3 : 1 ratio.

Examination Papers 197 This result is obtained due to segregation of the alleles at the time of gametogenesis. The alleles remain together in a zygote but during gamete formation, they segregate such that the gametes carry only one allele. 23. The property of plant cells that helped them to grow into a new plant is totipotency. The advantages of micropropagation are: (i) By this method, it is possible to achieve propagation of a large number of plants in very short durations. Plants like tomato, banana, apple, etc., have been produced on commercial scale. (ii) Healthy plants can be recovered from diseased plants by micropropagation. This is done by removing the meristem, which is disease-free and growing it in vitro. This has been done in banana, sugar cane, potato, etc. SECTIOND 30. (a) (i) The biogeochemical cycle shown is carbon cycle. (ii) Volcanic activity and mining/Microbial decomposition of organic matter. (b) Due to large scale deforestation, the flow of carbon in the environment will be disturbed because plants are the major consumers which utilise carbon for photosynthesis. This would lead to accumulation of carbon in the atmosphere. (c) Increase in level of carbon in atmosphere will result in greenhouse effect. This will lead to heating of earths surface and finally to global warming. Rise in temperature result in odd climatic changes called El Nino effects which are harmful for the environment. OR (a) Healthy ecosystems are the base for a wide range of economic, environmental and aesthetic goods and services. Some of these are as follows: (i) Purify air and water. (ii) Mitigate droughts and floods. (iii) Cycle nutrients. (iv) Generate fertile soils (v) Provide wildlife habitat (vi) Maintain biodiversity (vii) Pollinate crops. (viii) Provide storage site for carbon. (ix) Provide aesthetic, cultural and spiritual values. (b) Differences: Hydrarch succession takes place in wetter areas and the successional series progresses from hydric to the mesic conditions. Xerarch succession takes place in dry areas and the series progresses from xeric to mesic conditions. Similarity: Both successions lead to medium water conditions, neither too dry nor too wet.

SETIII
SECTIONA 3. Beyond a certain depth in the ocean, sunlight is not able to penetrate. Due to which green plants cannot photosynthesise and thus, do not survive.

198 Xam idea BiologyXII SECTIONB 9. (i) Deoxyribonucleoside triphosphates act as substrates for polymerisation. (ii) These provide energy for polymerisation reaction. 13. Ti plasmids are tumour inducing plasmids of Agrobacterium tumifaciens which have been modified into a cloning vector which is not pathogenic but uses the mechanisms to deliver genes of interest into the plant. 16. Primary lymphoid organs are the sites where immature lymphocytes differentiate into antigen sensitive lymphocytes. Secondary lymphoid organs are the sites where the lymphocytes interact with antigens and proliferate to become effector cells. SECTIONC 21. The DNA-dependent DNA polymerase catalyses polymerisation only in one direction i.e., 53. Therefore, in one strand with polarity 35 continuous replication takes place whereas the other strand with polarity 53 carries out discontinuous replication. The strand with polarity 35 is called leading strand and the strand with polarity 53 is called lagging strand. 25. (a) Chemical fertilisers have significantly increased the soil pollution, reducing its quality. Therefore, farmers use biofertilisers which enrich the quality of the soil. (b) Anabaena fix atmospheric nitrogen, thus enriching the nitrogen content of the soil. In mycorrhiza, the fungal symbiant absorbs phosphorus from soil and passes it to the plant. 26. (a) Plasmodium enters the human body as sporozoites. (b) When a female Anopheles mosquito bites an infected person, the parasites enter the mosquitos body and undergo further development. The parasites multiply within them to form sporozoites that are stored in salivary glands until their transfer to human body. (c) The rupture of RBCs release a toxic substance called haemozoin, which is responsible for the chill and high fever recur. OR Life cycle of HIV:
OO OO OO

OO OO

OO

OO

After getting into the body of a person, the virus enters the macrophages. Here, RNA is replicated to form viral DNA by enzyme reverse transcriptase. The viral DNA now gets incorporated into the host cells DNA and directs the infected cells to produce viruses. The macrophages continue to produce virus particles. The virus particles enter helper T-lymphocytes (TH cells) in the blood, where they continue to replicate and produce viral progenies. Thus, the number of helper T-lymphocytes progressively decreases in the body of the infected persons. With the decrease in number of T-cells, the immunity also decreases. The person is unable to produce any immune response even against common bacteria like Mycobacterium, parasites like Toxoplasma, viruses and fungi.

Examination Papers 199 SECTIOND 28. (a) Oogenesis consists of the following three phases: (i) Multiplication phase: Oogenesis is initiated during the embryonic development stage when a couple of million gamete mother cells (oogonia) are formed within each foetal ovary. No more oogonia are formed and added after birth. These cells start division and enter into prophase-I of the meiotic division. They get temporarily arrested at this stage and are called primary oocytes. (ii) Growth phase: Each primary oocyte then gets surrounded by a layer of granulosa cells. This structure is called the primary follicle. A large number of these follicles degenerate during the phase from birth to puberty. The primary follicles get surrounded by more layers of granulosa cells and a new theca to form secondary follicles. (iii) Maturation phase: In the first maturation phase, the secondary follicle soon transforms into a tertiary follicle. The primary oocyte within the tertiary follicle grows in size and completes its first meiotic division to form a large haploid secondary oocyte and a tiny first polar body. The tertiary follicle changes into a mature folliclethe Graafian folliclewhich ruptures to release the secondary oocyte (ovum) from the ovary by a process called ovulation. The second maturation phase occurs after fertilisation when the meiotic division of the secondary oocyte is complete. This second meiotic division results in the formation of a second polar body and a haploid ovum (ootid). (b)

(Ovum)

OR (a) Spermatogenesis occurs at puberty in testes. (b)

Structure of a sperm

200 Xam idea BiologyXII (c) Acrosome: Acrosome contains hydrolytic enzymes that help in dissolving membranes of the ovum for fertilisation. Middle piece: It contains a number of mitochondria that provide energy for the movement of the tail and provides motility to sperm.

zzz

CBSE Examination Papers (All India 2011)

Time: 3 Hours

Examination Papers 201

Max. Marks: 70

General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. SectionA contains 8 questions of 1 mark each. SectionB is of 10 questions of 2 marks each. SectionC is of 9 questions of 3 marks each and SectionD is of 3 questions of 5 marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.

SETI
SECTIONA 1. Name the embryonic stage that gets implanted in the uterine wall of a human female. 2. State the importance of biofortification. 3. Biotechnologists refer to Agrobacterium tumifaciens as a natural genetic engineer of plants. Give reasons to support the statement. 4. How do algal blooms affect the life in water bodies? 5. Name the common ancestor of the great apes and man. 6. Write a difference between net primary productivity and gross productivity. 7. Mention the contribution of genetic maps in human genome project. 8. Name the phase that all organisms have to pass through before they can reproduce sexually. SECTIONB 9. Name the enzyme produced by Streptococcus bacterium. Explain its importance in medical sciences. 10. How is Rosie considered different from a normal cow? Explain. 11. State the use of biodiversity in modern agriculture. 12. Write the full form of VNTR. How is VNTR different from probe? 13. Differentiate between benign and malignant tumours.

202 Xam idea BiologyXII 14.

Species richness a b

Area The above graph shows speciesarea relationship. Write the equation of the curve a and explain. OR Differentiate between in situ and ex situ approaches of conservation of biodiversity. 15. The cell division involved in gamete formation is not of the same type in different organisms. Justify. 16. Identify the type of the given ecological pyramid and give one example each of pyramid of number and pyramid of biomass in such cases.

17. Describe the lactational amenorrhea method of birth control. 18. Name the type of bioreactor shown. Write the purpose for which it is used.

SECTIONC 19. Draw a labelled diagram of the reproductive system in a human female. 20. Branching descent and natural selection are the two key concepts of Darwinian theory of evolution. Explain each concept with the help of a suitable example. 21. Scientists have succeeded in recovering healthy sugarcane plants from a diseased one. (a) Name the part of the plant used as explant by the scientists. (b) Describe the procedure that the scientists followed to recover the healthy plants. (c) Name this technology used for crop improvement.

Examination Papers 203 22. (i) Name the enzyme that catalyses the transcription of hnRNA. (ii) Why does the hnRNA need to undergo changes? List the changes that hnRNA undergoes and where in the cell such changes take place. 23. (i) Write the scientific names of the two species of filarial worms causing filariasis. (ii) How do they affect the body of infected person(s)? (iii) How does the disease spread? 24. Name the genus to which baculoviruses belong. Describe their role in the integrated pest management programmes. 25. Unambiguous, universal and degenerate are some of the terms used for the genetic code. Explain the salient features of each one of them. 26. Water is very essential for life. Write any three features both for plants and animals which enable them to survive in water scarce environment. OR How do organisms cope with stressful external environmental conditions which are localised or of short duration? 27. (i) State the consequence if the electrostatic precipitator of a thermal plant fails to function. (ii) Mention any four methods by which the vehicular air pollution can be controlled. SECTIOND 28. Give reasons why: (i) most zygotes in angiosperms divide only after certain amount of endosperm is formed. (ii) groundnut seeds are exalbuminous and castor seeds are albuminous. (iii) micropyle remains as a small pore in the seed coat of a seed. (iv) integuments of an ovule harden and the water content is highly reduced, as the seed matures. (v) apple and cashew are not called true fruits. OR (a) Draw a labelled diagram of L.S. of an embryo of grass (any six labels). (b) Give reason for each of the following: (i) Anthers of angiosperm flowers are described as dithecous. (ii) Hybrid seeds have to be produced year after year. 29. Describe the mechanism of pattern of inheritance of ABO blood groups in humans. OR (a) Why is haemophilia generally observed in human males? Explain the conditions under which a human female can be haemophilic. (b) A pregnant human female was advised to undergo M.T.P. It was diagnosed by her doctor that the foetus she is carrying has developed from a zygote formed by an XX egg fertilised by Y-carrying sperms. Why was she advised to undergo M.T.P.? 30. (i) Describe the characteristics that a cloning vector must possess. (ii) Why DNA cannot pass through the cell membrane? Explain. How is a bacterial cell made competent to take up recombinant DNA from the medium? OR If a desired gene is identified in an organism for some experiments, explain the process of the following:

204 Xam idea BiologyXII (i) Cutting this desired gene at specific location. (ii) Synthesis of multiple copies of this desired gene.

SETII (Questions Uncommon to SETI)

SECTIONA 3. Why is it essential to have a selectable marker in a cloning vector? SECTIONB 9. Why are some molecules called bioactive molecules? Give two examples of such molecules. 13. List the two types of immunity a human baby is born with. Explain the differences between the two types. 16. Explain the response of all communities to environment over time. SECTIONC 21. Describe the technology that has successfully increased the herd size of cattle in a short time to meet the increasing demands of growing human population. 23. (a) Name the causative agent of typhoid in humans. (b) Name the test administered to confirm the disease. (c) How does the pathogen gain entry into the human body? Write the diagonostic symptoms and mention the body organ that gets affected in severe cases. 25. (a) Name the scientist who called tRNA an adaptor molecule. (b) Draw a clover leaf structure of tRNA showing the following: (i) Tyrosine attached to its amino acid site. (ii) Anticodon for this amino acid in its correct site (codon for tyrosine is UCA). (c) What does the actual structure of tRNA look like? SECTIOND 30. (a) With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease enzyme EcoRI. (b) Name the technique that is used for separating the fragments of DNA cut by restriction endonucleases. OR (a) Name the source from which insulin was extracted earlier. Why is this insulin no more in use by diabetic people? (b) Explain the process of synthesis of insulin by Eli Lilly Company. Name the technique used by the company. (c) How is the insulin produced by human body different from the insulin produced by the above mentioned company?

SETIII (Questions Uncommon to SetI and SetII)

SECTIONA 1. What stimulates pituitary to release the hormone responsible for parturition? Name the hormone. 2. Pollinating species of wasps show mutualism with specific fig plants. Mention the benefits the female wasps derive from the fig trees from such an interaction.

Examination Papers 205 SECTIONB 9. A relevant portion of -chain of haemoglobin of a normal human is given below:

The codon for the sixth amino acid is GAG. The sixth codon GAG mutates to GAA as a result of mutation A and into GUG as a result of mutation B. Haemoglobin structure did not change as a result of mutation A whereas haemoglobin structure changed because of mutation B leading to sickle shaped RBCs. Explain giving reasons how could mutation B change the haemoglobin structure and not mutation A. 10. Biopiracy should be prevented. State why and how. 13. Why is tobacco smoking associated with rise in blood pressure and emphysema (oxygen deficiency in the body)? Explain. 18. What is polyblend? Why did the plastic manufacturers think of producing it? Write its usefulness. SECTIONC 23.

206 Xam idea BiologyXII Study the diagram showing replication of HIV in humans and answer the following questions accordingly:

(i) Write the chemical nature of the coat A. (ii) Name the enzyme B acting on X to produce molecule C. Name C. (iii) Mention the name of the host cell D the HIV attacks first when it enters into the human body. (iv) Name the two different cells that the new viruses E subsequently attack. 25. Answer the following questions based on Meselson and Stahls experiment: (a) Write the name of the chemical substance used as a source of nitrogen in the experiment by them. (b) Why did the scientists synthesise the light and the heavy DNA molecules in the organism used in the experiment? (c) How did the scientists make it possible to distinguish the heavy DNA molecule from the light DNA molecule? Explain. (d) Write the conclusion the scientists arrived at after completing the experiment. SECTIOND 29. (a) State the arrangement of different genes that in bacteria is referred to as operon. (b) Draw a schematic labelled illustration of lac operon in a switched on state. (c) Describe the role of lactose in lac operon. OR (a) A true breeding homozygous pea plant with green pods and axial flowers as dominant characters, is crossed with a recessive homozygous pea plant with yellow pods and terminal flowers. Work out the cross up to F2 generation giving the phenotypic ratios of F1 and F2 generation respectively. (b) State the Mendelian principle which can be derived from such a cross and not from monohybrid cross.

Examination Papers 207

SOLUTIONS
SETI
SECTIONA 1. Blastocyst/blastula. 2. (i) Breeding of crops for improvement of nutritional quality. (ii) Higher level of vitamins/proteins/minerals/healthier fats. (Any one) 3. This is because A. tumifaciens can transfer genes naturally by delivering a piece of T-DNA. It has a tumour inducing plasmid. 4. (i) Algal bloom pollutes water and deteriorates the water quality by depleting the oxygen content. This turns the water toxic and increase its BOD. 5. Dryopithecus/Ramapithecus. 6. Gross productivity (GPP) is the rate of production of organic matter during photosynthesis. Net primary productivity (NPP) is the available biomass for the consumption by heterotrophs. GPP R = NPP 7. Sequencing of genes, DNA fingerprinting, tracing human history, chromosomal location for disease associated sequences (Any one) 8. Juvenile/growth phase. SECTIONB 9. Streptococcus bacterium produces streptokinase. It is used for removing clots from the blood vessels in a patient suffering from myocardial infarction/or in a heart patient. 10. Rosie is a transgenic cow. 11. Biodiversity is a source of hybrids, GM plants, biopesticides, organic farming, biofertiliser, improved varieties of plants, disease resistant plants. (Any two) 12. VNTR stands for Variable Number of Tandem Repeats. Rosie produced human protein-enriched milk containing human alpha-lactalbumin.

(ii) Algal blooms lead to death of aquatic organisms due to oxygen depletion.

Probe is labelled or radioactive (single stranded polynucleotide that hybridises DNA fragments). 13.
S. No. (i) (ii) (iii) (iv) Benign Tumour It is non-cancerous. Remains confined. No metastasis. Causes limited damage. Malignant Tumour It is cancerous. Spreads to other parts of the body. Shows metastasis. Causes serious damage. (Any two comparisons)

208 Xam idea BiologyXII 14. The equation of the curve a is S = CAZ. (i) Within a region, species richness increases with increasing explored area but only up to a limit. (ii) Relationship between species richness and area for a wide variety of taxa turns out to be rectangular hyperbola. OR
S. No. (i) (ii) In situ Approach It involves protection of endangered species of plants and animals. This is done by protecting the natural habitat or ecosystem. Ex situ Approach It involves protection of endangered species by removing them from the natural habitat This is done by placing the species under special care.

15. The parents may be haploid or diploid but the gametes always have to be haploid. Diploid parents undergo meiosis to produce haploid gametes. Whereas haploid parents undergo mitosis to produce haploid gametes. 16. The given ecological pyramid is the inverted pyramid. Inverted pyramid of biomass in a lake: Phytoplankton Zooplankton fishes. Inverted pyramid of number: Tree insects birds. 17. Lactational amenorrhea is based on the principle that during the period of intense lactation after parturition, menstrual cycle or ovulation does not occur. 18. The given bioreactor is the simple stirred tank bioreactor. Its purpose is large scale production of recombinant protein or enzymes, using microbial plants/animals/human cells. SECTIONC 19.

Diagrammatic sectional view of the female reproductive system

Examination Papers 209 20. Branching descent: Different species descending from the common ancestor get adapted in different habitats, e.g., Darwins finchesvarieties of finches arose from grain eaters; Australian marsupials evolved from common marsupial. Natural selection: It is a process in which heritable variations enable better survival of a species to reproduce in large number, e.g., white moth surviving before the industrial revolution and black moth surviving after industrial revolution; long-necked giraffe survived the evolution process; DDT-resistant mosquitoes survive. (Any suitable example) 21. (a) Meristem (apical, axillary) (b) The explant or virus-free meristem is grown in nutrient medium under aseptic conditions. The tissue proliferates to form undifferentiated mass/callus. This callus formed is transferred to a medium containing growth hormones like auxins and cytokinins. (c) Tissue culture or micropropagation. 22. (i) RNA polymerase II. (ii) hnRNA has non-functional introns in between the functional exons. To remove these, it undergoes changes. The changes that hnRNA undergoes include capping, i.e., methyl guanosine triphosphate is added to 5 end; tailing in which poly A tail is added at 3 end; and splicing by which introns are removed and exons are joined. 23. (i) Wuchereria bancrofti, Wuchereria malayi. (ii) Filariasis results in inflammation of the lymphatic vessels of the lower limbs and of the genital organs. It can also lead to gross deformity of the lower limbs and the genital organs (Any two). (iii) Through the bite of female Culex mosquito. 24. Baculoviruses belong to the genus Nucleopolyhedrovirus. They control only species-specific pest, do not affect non-target organisms or beneficial insects are conserved, they aid in IPM problems and there is no negative impact on plants or other animals. 25. Unambiguous code means that one codon codes for only one amino acid, e.g., AUG codes for only methionine.

Universal code means that codon and its corresponding amino acid are the same in all organisms, e.g., from bacteria to human, UUU codes for phenylalanine. Degenerate code means that some amino acids are coded by more than one codon, e.g., UUU and UUC code for phenylalanine. 26. Plants: Ephemeral mode (complete life cycle in short period); deep tap roots; deciduous leaves; waxy cuticle; sunken stomata; succulence to store water; C4 or CAM pathway of photosynthesis. (Any three)

Animals: No sweating; uricotelic; deposition of fat in sub-epidermal layer; burrowing nature; thick skin; body covered with scales. (Any three) OR The following methods are employed by organisms to cope with stressful conditions: (i) Migrate temporarily from the stressful habitat to a hospitable area, (ii) suspend activities,

210 Xam idea BiologyXII (iii) form thick walled spores, (iv) form dormant seeds, (v) hibernate during winter, (vi) aestivate during summer, (vii) planktons undergo diapause. (Any six) 27. (i) Particulate matter will pollute the air. (ii) Vehicular pollution can be controlled by: (a) Use of CNG, (b) Phasing out of old vehicles, (c) Use of unleaded petrol, (d) Use of low sulphur fuel, (e) Use of catalytic converters, (f) Application of stringent pollution level norms. (Any four) SECTIOND 28. (i) To obtain nutrition from the endosperm for the developing embryo. (ii) The groundnut seeds are exalbuminous because the endosperm is completely consumed. Whereas, castor seeds are albuminous because the endosperm persists. (iii) For the entry of water and oxygen required for germination. (iv) To protect the embryo and keep the seed viable, until favourable conditions return for germination. (v) In apple and cashew, ovary does not take part in fruit formation, instead thalamus contributes to fruit formation. OR (a)
Scutellum Coleoptile Shoot apex Epiblast

Radicle Root cap Coleorrhiza

L.S. of an embryo of grass (monocot)

(b) (i) Anthers of angiosperms are described as dithecous because each bilobed anther has two theca. (ii) Hybrid seeds have to be produced year after year because progeny shows segregation and do not maintain hybrid characters.

Examination Papers 211 29. Human blood group is determined by glycoprotein/antigen A and glycoprotein/antigen B. The alleles are IA, IB and i. Hence, referred to as multiple allelism. The individual inherits any two of them as given below. IAIA, IAi A group IBIB, IBi B group A B I I AB group ii O group In the case of A, B and O groups, Law of dominance is the pattern of inheritance as IA and IB are dominant over i. In AB group both the alleles IA and IB express themselves. Thus, it is the case of co-dominance. OR (a) Haemophilia is caused due to the recessive gene on X-chromosome. Y has no allele for this. If a male is XhY, then he is haemophilic. If male inherits Xh from the mother, he will be haemophilic (with the genotype XhY). If female inherits XhXh, one from the carrier mother and one from her haemophilic f ather, then she can be haemophilic. (b) Embryo has (trisomy of sex chromosome) XXY karyotype or Klinefelters syndrome. She was advised to undergo MTP since the child will have the following problems: (i) male with feminine traits (ii) gynaecomastia (iii) underdeveloped testes (iv) sterile 30. (i) A cloning vector must have the following characteristics: (a) ori or origin of replication (b) Selectable marker, genes encoding for an antibiotic resistance or genes encoding for -galactosidase. (c) Cloning site or recognition site for the restriction enzyme to recognise. (ii) DNA is a hydrophilic molecule, therefore it cannot pass through the cell membrane. The bacterial cells can be made competent by treating them with a specific concentration of a divalent ion like calcium. The cells are then incubated on ice followed by a heat shock by placing them briefly at 42C and then putting back on ice. OR (i) The desired gene is cut using the enzymes restriction endonucleases. Firstly, the restriction endonucleases that recognise the palindromic nucleotide sequence of the desired gene is identified. The endonuclease inspects the entire DNA sequences to find and recognise the site. It cuts each of the double helix at a specific point which is a little away from the centre of the palindromic site. The cutting site is between the same two bases on the opposite strands. This results in over-hanging single stranded stretches which act as sticky ends. (ii) Multiple copies of the desired gene is synthesised by polymerase chain reaction (PCR) method. In this method, the desired gene is synthesised in vitro. The double stranded DNA is denatured using high temperature of 95C and the strands are separated. Each separated strand acts as template.

212 Xam idea BiologyXII Two sets of oligonucleotide primers are annealed to the denatured DNA strands. The thermostable Taq polymerase extends the primers using nucleotides provided in the reaction mixture. Finally the amplified fragments are ligated into recipient cells.

SETII
SECTIONA 3. Selectable markers are essential to identify and eliminate non-transformants, to selectively permit the growth of the transformant. SECTIONB 9. This is because microbes like bacteria or fungi are used in their production. e.g., Citric acid Acetic acid Butyric acid Lactic acid Ethanol Lipase Streptokinase Cyclosporin A (Any two) 13. The two types of immunity are innate and passive/acquired immunity. Innate immunity is a non-specific type of defence that provides barriers to the entry of antigens. Passive immunity is a pathogen-specific type of defence that develops in response to encouter with pathogen. The foetus recieves antibodies through the placenta. 16. Environmental factors like temperature, water, light, soil, etc., may influence the members of communities in varying degrees. Organisms in response to these factors shall try to adapt according to their capacities. In this process, they may try to maintain a constant internal environment through homeostasis or migrate to a less stressful environment or may even suspend activities till favourable conditions return. SECTIONC 21. Multiple ovulation embryo transfer technology (MOET) has successfully increased the herd size of cattle. The cow is administered with FSH to induce follicular maturation and super-ovulation to produce 6 to 8 eggs. It is then mated or artificially inseminated. The fertilised eggs are recovered non-surgically and transferred to surrogate mother where they develop into an improved variety. 23. (a) Salmonella typhi (b) Widal test (c) The pathogen gains entry through contaminated food and water. The symptoms include sustained high fever (30C to 40C), weakness, stomach pain, constipation, headache, loss of appetite (Any two). In severe cases, intestine gets affected.

Examination Papers 213 25. (a) Francis Crick (b)

Tyrosine

U C A (c) The actual structure of tRNA looks like inverted L.

SECTIOND 30. (a)
EcoRI

EcoRI

Steps in formation of recombinant DNA by action of restriction endonuclease enzyme EcoRI

(b) Gel electrophoresis is used for separating the fragments of DNA cut by restriction endonucleases.

214 Xam idea BiologyXII OR (a) Earlier, insulin was extracted from pancreas of slaughtered cattle and pig. This insulin is not in use as some patients developed allergic reaction to this foreign protein. (b) Eli Lilly used the following procedure for insulin synthesis: (i) Two DNA sequences corresponding to A and B chains of insulin were prepared. (ii) These sequences were then introduced in plasmids of E. coli. (iii) The two insulin chains are produced separately. (iv) The two chains are extracted and combined by creating disulphide bonds to form the assembled mature molecule of insulin. (c) The pro-hormone produced in the human body has an extra stretch of C peptide.

SETIII
SECTIONA 1. The signal from the fully developed foetus and placenta or the foetal ejection reflex induces mild uterine contraction. The hormone released is oxytocin. 2. The wasp uses the fruit as oviposition and the developing seeds for nourishing its larvae. SECTIONB 9. Due to mutation A, GAG mutates to GAA. Both GAG and GAA code for glutamic acid and hence there is no change in RBCs. Whereas GUG formed due to mutation B codes for valine and so the RBCs become sickle-shaped. 10. Biopiracy is unauthorised exploitation of bioresources of developing or under-developed countries. Hence, it should be prevented. It can be prevented by developing laws to obtain proper authorisation and by paying compensatory benefits. 13. Tobacco has nicotine that stimulates the release of adrenaline and noradrenaline which raise blood pressure. Smoking tobacco releases carbon monoxide which reduces the concentration of haem-bound oxygen. This causes emphysema. 18. Polyblend is a fine powder of recycled modified plastic. Polyblend was produced to recycle plastic waste. When blended with bitumen, polyblend can be used to lay roads that have increased road life. SECTIONC 23. (i) Coat A is made up of protein. (ii) The enzyme B is reverse transcriptase, C is viral DNA. (iii) The host cell D is macrophage. (iv) The new viruses E subsequently attack macrophages and helper T-lymphocytes. 25. (a) Ammonium chloride (NH4Cl). (b) To check if DNA replication was semi-conservative.

Examination Papers 215 (c) The heavy and light DNA molecules were distinguished by centrifugation in a cesium chloride density gradient. (d) The scientists concluded that DNA replicates semi-conservatively. SECTIOND 29. (a) The operon has polycistronic structural genes, i.e., three structural genes adjacent to an operator, a promoter and a regulator. (b)

Initiation, or first codon

Trp

Process of translation

(c) Lactose is the inducer that inactivates repressor. This allows RNA polymerase to access promoter and initiate transcription of the structural genes or switch on the operon.

216 Xam idea BiologyXII OR (a)

(b) From the above cross law of independent assortment can be derived which states that when two pairs of traits are combined in a hybrid, segregation of one pair of character is independent of the other pair of characters.

zzz

CBSE Examination Papers (Foreign 2011)

Time: 3 Hours Max. Marks: 70 General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. SectionA contains 8 questions of 1 mark each. SectionB is of 10 questions of 2 marks each. SectionC is of 9 questions of 3 marks each and SectionD is of 3 questions of 5 marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.

SETI
SECTIONA 1. List the changes that the primary ooctye undergoes in the tertiary follicular stage in the human ovary. 2. Why are cattle and goats not seen browsing on Calotropis growing in the fields? 3. Mention how is mutation theory of Hugo de Vries different from Darwins theory of natural selection. 4. Why are some organisms called as eurythermals and some others as stenohaline? 5. Mention the type of allele that expresses itself only in homozygous state in an organism. 6. Malaria, typhoid, pneumonia and amoebiasis are some of the human infectious diseases. Which ones of these are transmitted through mechanical carriers? 7. Name any two techniques that serve the purpose of early diagnosis of some bacterial/viral human diseases. 8. Name the phenomenon and the cell responsible for the development of a new individual without fertilisation as seen in honey bees. SECTIONB 9. In a dihybrid cross white eyed, yellow bodied female Drosophila crossed with red eyed, brown bodied male Drosophila produced in F2 generation 1.3 per cent recombinants and 98.7 per cent progeny with parental type combinations. This observation of Morgan deviated from Mendelian F2 phenotypic dihybrid ratio. Explain, giving reasons, Morgans observations. 10. How are recombinant vectors created? Why is only one type of restriction endonuclease required for creating one recombinant vector?

218 Xam idea BiologyXII 11. Bear hibernates whereas some species of zooplanktons enter diapause to avoid stressful external conditions. How are these two ways different from each other? 12. The figure below shows the relative contribution of four greenhouse gases to global warming:

(i) Identify the gases A and C. (ii) Why are these four gases called the greenhouse gases? 13. At the time of Independence, the population of India was 350 million, which exploded to over 1 billion by May 2000. List any two reasons for this rise in population and any two steps taken by the government to check this population explosion. 14. Explain the function of reservoir in a nutrient cycle. List the two types of nutrient cycles in nature. OR Explain with the help of two examples, how the pyramid of numbers and the pyramid of biomass can look inverted. 15. A list of three flowering plants is given below. Which ones out of them are (i) monoecious and (ii) bearing pistillate flowers: List: Datepalm, Cucurbits and Pea. 16. Name the organism from where the thermostable DNA polymerase is isolated. State its role in genetic engineering. 17. Study the flow chart given below: Cow is administrated with FSH hormone (1) 68 eggs per cycle are derived Artificially inseminated Fertilised eggs at 832 cells are recovered (2)

Examination Papers 219 (i) Identify the events that take place at stages (1) and (2) respectively. (ii) State the importance of the technology explained above. 18. Give the scientific name of the microbes from which cyclosporin A and statin are obtained. Write one medical use of each one of these drugs. SECTIONC 19. Draw a labelled diagram of the microscopic structure of a human sperm. 20. Convergent evolution and divergent evolution are the two concepts explaining organic evolution. Explain each one with the help of an example. 21. (a) Name a drug used (i) as an effective sedative and pain killer (ii) for helping patients to cope with mental illnesses like depression, but often misused. (b) How does the moderate and high dosage of cocaine affect the human body? 22. Given below is a schematic representation of lac operon:
1

(a) Identify i and p. (b) Name the inducer for this operon and explain its role. 23. Explain the different steps involved in sewage treatment before it can be released into natural water bodies. 24. Study the three different age pyramids for human population given below and answer the questions that follow:

(a) Write the names given to each of these age pyramids. (b) Mention the one which is ideal for human population and why. 25. Name a disorder, give the karyotype and write the symptoms a human suffers from as a result of monosomy of the sex chromosome. 26. Explain, giving one example, how co-extinction is one of the causes of loss of biodiversity. List the three other causes also (without description). OR Eutrophication is the natural aging of a lake. Explain. 27. IARI has released several varieties of crop plants that are biofortified. Give three examples of such crops and their biofortifications.

220 Xam idea BiologyXII SECTIOND 28. (a) Draw a diagram of an enlarged view of T.S. of one microsporangium of an angiosperm and label the following parts: (i) Tapetum (ii) Middle layer (iii) Endothecium (iv) Microspore mother cells (b) Mention the characteristic features and function of tapetum. (c) Explain the following giving reasons: (i) Pollen grains are well preserved as fossils. (ii) Pollen tablets are in use by people these days. OR (a) Why is the process of fertilisation in angiosperms termed as double fertilisation? Explain. (b) Draw a diagram of an angiospermic embryo sac where fertilisation is just completed. Label the following parts: (i) Micropylar end of the embryo sac. (ii) The part that develops into an embryo. (iii) The part that develops into an endosperm. (iv) The degenerating cells at the chalazal end. (c) Draw a labelled diagram of globular embryonic stage of an angiosperm. 29. Name the process involved in the production of nematode-resistant tobacco plants, using genetic engineering. Explain the strategy adopted to develop such plants. OR Describe the various stages involved in gene transfer for the commercial production of human insulin by Eli Lilly. 30. (a) Explain the process of aminoacylation of tRNA. Mention its role in translation. (b) How do ribosomes in the cells act as factories for protein synthesis? (c) Describe initiation and termination phases of protein synthesis. OR (a) Explain the role of DNA dependent RNA polymerase in initiation, elongation and termination during transcription in bacterial cell. (b) How is transcription a more complex process in eukaryotic cells? Explain.

SETII (Questions Uncommon to SETI)

SECTIONA 1. Name the group of organisms that produce non-motile gametes. How do they reach the female gamete for fertilisation? 5. State which human chromosome has (i) the maximum number of genes and (ii) the one which has the least number of genes?

Examination Papers 221 7. Mention the source organism of the gene cryIAc and its target pest. SECTIONB 10. Study the diagram given below and answer the questions that follow:

(a) Why have DNA fragments in band D moved farther away in comparison to those in band C? (b) Identify the anode end in the diagram. (c) How are these DNA fragments visualized? 11. How does our body adapt to low oxygen availability at high altitudes? SECTIONC 19. Describe how the changing levels of FSH, LH and progesterone during menstrual cycle induce changes in the ovary and the uterus in human female. 23. Name a genus to which baculoviruses belong. Describe their role in the integrated pest management. 27. Given below is a diagrammatic representation of the experimental set-up used by S.L. Miller for his experiment:

222 Xam idea BiologyXII (a) Write the names of different gases contained and the conditions set for the reaction in the flask A. (b) State the type of organic molecule he collected in the water at B. (c) Write the conclusion he arrived at. SECTIOND 30. (a) Write the scientific name of the bacterium used by Frederick Griffith in his experiment. (b) How did he prove that some transforming principle is responsible for transformation of the non-virulent strains of bacteria into the virulent form? (c) State the biochemical nature of transforming principle. (d) Name the scientists who proved it. OR The average length of a DNA double helix in a typical mammalian cell is approximately 2.2 metres and the dimension of the nucleus is about 106 m. (a) How is it possible that long DNA polymers are packed within a very small nucleus? (b) Differentiate between euchromatin and heterochromatin. (c) Mention the role of non-histone chromosomal protein.

SETIII (Questions Uncommon to SETI and SETII)

SECTIONA 3. What does Hardy-Weinberg equation p2 + 2pq + q2 = 1 convey? SECTIONB 9. Haemophilia is a sex-linked inheritance condition in humans where a simple cut causes non-stop bleeding. Study the pedigree chart showing the inheritance of haemophilia in a family. Answer the questions that follow:

Give reasons which explain that haemophilia is (i) sex-linked, and (ii) caused by X-linked gene. 13. Name the hormones produced only during pregnancy in a human female. Mention their source organ. 16. (a) Why are restriction endouncleases known as molecular scissors? (b) Write the convention used for naming such enzymes.

Examination Papers 223 SECTIONC 21. Explain the role of the following in providing defence against infection in human body: (i) Histamines (ii) Interferons (iii) B-cells 25. Name a disorder, give the karyotype and write the symptoms where a human male suffers as a result of an additional X-chromosome. 26. Explain rivet popper hypothesis. Name the ecologist who proposed it. SECTIOND 28. (a) Explain the characteristic features of wind pollinated flowers. How are insect pollinated flowers different from them? (b) Explain the mutually rewarding relationship between Yucca plant and a species of moth. OR (a) Name the structures which the parts A and B shown in the diagram below respectively develop into. (b) Explain the process of development which B undergoes in albuminous and exalbuminous seeds. Give one example of each of these seeds.

224 Xam idea BiologyXII

SOLUTIONS
SETI
SECTIONA 1. The primary oocyte within the tertiary follicle grows in size and completes its first meiotic division. 2. Calotropis produces highly poisonous cardiac glycosides. Therefore, cattle and goats do not browse on them. 3. According to Hugo de Vries, new species arise due to single step large mutation whereas according to Darwin, evolution occurs gradually by the method of natural selection. 4. Eurythermals are organisms that can tolerate and thrive in a wide range of temperature, whereas stenohalinees can tolerate a narrow range of salinities. 5. Recessive allele. 6. Malaria and amoebiasis are transmitted through mechanical carriers. 7. ELISA and PCR serve the purpose of early diagnosis of human diseases. 8. The phenomenon is called parthenogenesis and the cell responsible for it is female gamete, ovum. SECTIONB 9. Morgan saw that when the two genes in a dihybrid cross were situated on the same chromosome, the proportion of parental gene combinations were much higher than the non-parental type. Morgan attributed this due to physical association or linkage of two genes and coined the term linkage to describe this physical association of genes on a chromosome and the term recombination to describe the generation of non-parental gene combinations. 10. The construction of recombinant DNA is done by linking a gene encoding antibiotic resistance with a native plasmid. These plasmid DNA act as vectors to transfer the piece of DNA attached to it. Only one type of restriction endonuclease is required for creating recombinant vector because when cut by the same enzyme, the resultant DNA fragments have the same sticky ends, which can be joined together using DNA ligases. 11. Hibernation is the winter sleep seen in cold-blooded animals in polar regions in which they suspend their metabolic activities when external temperature becomes unfavourable. Whereas, diapause is the phenomenon seen in insects during their developmental stages, in which metabolic activities are suspended due to unfavourable conditions. 12. (i) Gas ACO2 Gas CCFCs (ii) These gases absorb the heat radiated from the Earth surface and thus retains the heat of Earth. These are responsible for this greenhouse effect, therefore they are called greenhouse effect. 13. Two reasons for increase in population are: (i) A rapid decline in death rate, maternal mortality rate and infant mortality rate. (ii) Increase in number of people in reproducible age.

Examination Papers 225 Two reasons for decline in population are: (i) Statutory raising of marriageable age of the female to 18 years and male to 21 years. (ii) Incentives given to couples with small families. 14. The function of the reservoir is to meet the deficit which occurs due to imbalance in the rate of influx and efflux. The two types of nutrient cycles are: (i) Gaseous (ii) Sedimentary. OR The pyramid of biomass in sea is generally inverted because the biomass of fishes far exceeds that of phytoplankton and the number of big fishes eating the small fishes is also greater than the small ones. Also in pyramid of number, the number of insects feeding on a big tree is far greater than the tree. Now the number of small birds depending on the insects and the number of larger birds eating the smaller ones also increases in the order. 15. (i) Monoecious plantCucurbits (ii) Bearing pistillate flowersDate palm. 16. The organism from where the thermostable DNA polymerase is isolated is Thermus aquaticus. It has wide role in genetic engineering as it is used in PCR because it can withstand high temperatures, which is applied to separate DNA strands. 17. (i) Events taking place at: (a) stage (1) Follicular maturation (b) Transfer to surrogate mothers (ii) Due to this technology, high milk-yielding breeds of cows and high quality meat yielding bulls have been bred successfully to increase herd size in a short time. 18. Cyclosporin A that is used as an immuno-suppressive agent in organ transplant patients is produced by the fungus Trichoderma polysporum. Statins produced by yeast Monococcus pupureus have been commercialised as blood cholesterol lowering agents. SECTIONC 19.

Structure of a sperm

226 Xam idea BiologyXII 20. Animals like whales, bats, cheetah and humans share similarities in the pattern of bones of forelimbs. In these animals, the same structure is developed along different directions due to adaptions to different needs. This is divergent evolution. Whereas in convergent evolution, the different structures evolve for the same function and have similarity. An example is the eye of octopus and of mammals or flippers of penguins and dolphins. 21. (a) (i) Morphine (ii) Lysergic acid diethyl amides (LSD). (b) Cocaine has a potent simulating action on central nervous system producing a sense of euphoria and increased energy. Excessive dosage of cocaine causes hallucinations. 22. (a) i = regulatory gene p = promoter (b) Lactose is the inducer. It is the substrate for the enzyme -galactosidase and regulates switching on and off of the operon. On entering the cell, lactose binds to the repressor to inactivate it. RNA polymerase now binds to the promoter to synthesise DNA. 23. Sewage treatment is carried out in two stages: (i) Primary treatment or physical treatment OO It is the physical removal of large and small particles from sewage. OO First, the floating debris is removed by sequential filtration by passing through wire mesh screens. OO Then, the grit (soil and small pebbles) are removed by sedimentation in settling tanks. The sediment is called primary sludge and the supernatant is the effluent. OO The effluent is taken for secondary treatment. (ii) Secondary treatment or biological treatment OO Primary effluent is passed into large aeration tanks with constant mechanical agitation and air supply. OO Useful aerobic microbes grow rapidly and form flocs. OO The growing microbes consume organic matter and thus reduce the biochemical oxygen demand (BOD). OO When BOD of sewage has reduced, the effluent is passed into settling tank. OO Here, the bacterial flocs settle and the sediment is called activated sludge. OO A small part of the sludge is used as an inoculum in the aeration tank and the remaining part is passed into large tanks called anaerobic sludge digesters. OO In the digesters, heterotrophic microbes anaerobically digest bacteria and fungi in sludge producing mixture of gases such as methane, hydrogen sulphide and CO2 which form the biogas. OO The effluent can now be released into the water body. 24. (a) A Expanding pyramid B Stable pyramid C Declining pyramid (b) Stable pyramid is ideal for human population because it maintains the stability in all population phases.

Examination Papers 227 25. Turners syndrome is a disorder caused by the absence of one of the X-chromosomes. Its karyotype will be 45 + XO. Symptoms: (i) Sterile females (ii) Rudimentary ovaries (iii) Lack of secondary sexual characters. 26. When a species becomes extinct, the plant and animal species associated with it in an obligatory may also become extinct. This is called co-extinction. For example, when a host fish species becomes extinct, its unique assemblage of parasites also becomes extinct. The three other causes are: (i) Habitat loss and fragmentation (ii) Over-exploitation (iii) Alien species invasion. OR Eutrophication is the natural aging of a lake by biological enrichment of its water. In a young lake, the water being cold and clear, does not support much life. But with time, streams draining into the lake introduce nutrients such as nitrogen and phosphorus, which encourage the growth of aquatic organisms. As the lakes fertility increases, plant and animal life begins to develop and organic remains begin to be deposited on the lakes bottom. Over the centuries, as silt and organic debris pile up, the lake grows shallower and warmer. Now, the warm water organisms replace those that live in a cold environment. Marsh plants take root in the shallows and begin to fill in the original lake basin. Eventually, the lake develops large masses of floating plants (bog), finally converting into land. 27. (i) Bittergourd enriched in vitamin C. (ii) Carrots enriched in vitamin A. (iii) Spinach enriched in iron and calcium. SECTIOND 28. (a)

Enlarged view of one microsporangium showing wall layers

228 Xam idea BiologyXII (b) Tapetum is the innermost wall layer of the microsporangium. It nourishes the developing pollen grains. Cells of the tapetum possess dense cytoplasm and generally have more than one nucleus. (c) (i) Pollen grains have thick outer exine which is composed of sporopollenin. Sporopollenin can withstand high temperatures and strong acids and alkalis. Therefore, they are well preserved as fossils. (ii) Pollen tablets are used by people because pollens are rich in nutrients. OR (a) On reaching synergid, pollen tube releases the two male gametes into cytoplasm of synergid. One of the male gamete fuses with egg nucleus to form a diploid zygote. This is called syngamy. Other male gamete fuses with polar nuclei at the centre to produce a triploid primary endosperm nucleus (PEN). This is termed as triple fusion. As syngamy and triple fusion take place simultaneously in the embryo sac, it is termed as double fertilisation. (b) Fertilised embryo sac showing zygote and primary endosperm nucleus (PEN)

Examination Papers 229 (c) Stages in embryo development in a dicot

29. The process involved in the production of nematode-resistant plants is RNA interference or RNAi. Using Agrobacterium vectors, nematode -specific genes were introduced into the host plant. The introduction of DNA was such that it produced both sense and antisense RNA in the host cells. These two RNAs being complementary to each other formed a double stranded RNA (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant therefore got itself protected from the parasite. OR (a) Eli Lilly prepared two DNA sequences corresponding to the A and B chains of human insulin, by the process of reverse transcription. (b) Sticky ends were produced in the Escherichia coli plasmid and the insulin gene by treating them both with the same restriction endonucleases. (c) These two are then joined together by the enzyme DNA ligase. (d) The bacteria are then grown in sterilised bioreactors in the appropriate growth medium. (e) The chains A and B are produced separately, extracted and purified. (f) These two chains are then combined by creating disulfide bonds to form human insulin. 30. (a) Aminoacylation is the process by which amino acids become activated by binding with its aminoacyl tRNA synthetase in the presence of ATP. If two charged tRNAs come close during translation process, the formation of peptide bond between them in energetically favourable. (b) The cellular factory responsible for synthesising proteins is the ribosome. The ribosome consists of structural RNAs and about 80 different proteins. In its inactive state, it exists as two subunits: a large subunit and a small subunit. When the small subunit encounters an mRNA, the process of translation of the mRNA to protein begins. There are two sites in the large subunit, for subsequent amino acids to bind

230 Xam idea BiologyXII to and thus, be close enough to each other for the formation of a peptide bond. The ribosome also acts as a catalyst (23S rRNA in bacteria is the enzyme-ribozyme) for the formation of peptide bond. (c) Initiation: (i) In prokaryotes, initiation requires the large and small ribosome subunits, the mRNA, initiation tRNA and three initiation factors (IFs). (ii) Amino acids become activated by binding with its aminoacyl-tRNA synthetase in the presence of ATP AA + ATP
amino acyl t-RNA synthetases

AA-AMP-Enzyme complex + PP

(iii) The AA-AMP-Enzyme complex formed react with specific tRNA to form amino acyl-tRNA complex. AA-AMP-Enzyme complex + tRNA (v) The ribosome have two sites A-site and P-site. (vi) The smaller subunit first binds the initiator tRNA and then attain to larger subunit so that initiation codon (AUG) come on the P-site. (vii) The initiation tRNA, i.e., methionyl tRNA binds to the P-site. Termination of polypeptide (i) When the A-site of ribosome reach a termination codon which does not code for any amino acid as a result, no charged tRNA bind to A-site. (ii) Dissociation of polypeptide from ribosome takes place which is catalysed by a release factor. (iii) There are three termination codons namely UGA, UAG and UAA. OR (a) The DNA dependent RNA polymerase helps in DNA replication by catalysing the polymerisation in only one direction, i.e., 53. In bacteria, the RNA polymerase has co-factors b, b, a, a, w and s which catalyse the process. Initiation: s (sigma) factor recognises the start signal and promotor region on DNA and the s (sigma) with RNA polymerase binds to the promoter and initiate transcription. Elongation: The RNA polymerase after initiation of RNA transcription loses the s-factor but continues the process of RNA formation. Termination: Once the RNA polymerase reaches the termination region of DNA, the RNA polymerase is separated from DNARNA hybrid, as a result nascent RNA separates. This process is called termination which is facilitated by certain termination factor . (b) The structural genes are monocistronic in eukaryotes whereas they are polycistronic in prokaryotes. In eukaryotes, coding gene sequences are removed from the non-coding sequences during RNA splicing whereas in prokaryotes AA-tRNA + AMP + Enzyme (iv) The cap region of mRNA bind to the smaller subunit of ribosome.

Examination Papers 231 there is no post-transcriptional modification. In eukaryotes, three types of RNA polymerases are found in the nucleus whereas in prokaryotes there is only one RNA polymerase.

SETII
SECTIONA 1. Angiosperms produce non-motile gametes. They reach the female gamete with the help of air or water. 5. (i) Chromosome-1 (ii) Y-Chromosome 7. Source organism Bacillus thuringiensis SECTIONB 10. (a) DNA fragments in band D are smaller in size than fragments in band C. Therefore, they moved faster and farther away. Target pest Cotton bollworms

(b) The anode end is B. (c) The separated DNA fragments can be visualised by staining the DNA with ethidium bromide followed by exposure to UV radiation. 11. Our body adapts to low oxygen availability by increasing red blood cell production, decreasing the binding capacity of haemoglobin and by increasing breathing rate. SECTIONC 19. The secretion of gonadotropins, i.e., LH and FSH increase gradually during the follicular phase, which stimulate follicular development as well as secretion of estrogens by the growing follicles. The rapid secretion of LH during mid-cycle induces rupture of Graafian follicle and thereby release of ovum. The corpus luteum, developed from Graafian follicle, secretes large amounts of progesterone which is essential for maintenance of endometrium for implantation. 23. Baculovirus belongs to the genus Nucleopolyhedrovirus. Baculoviruses are pathogens that attack insects and other arthopods. These viruses are very useful for species-specific, narrow spectrum insecticidal applications. Also, as they show no negative impacts on plants, mammals, birds, fish or even on non-target insects, they are beneficial in integrated pest management (IPM) programme in which beneficial insects are conserved. 27. (a) The gases obtained are CH4, H2, NH3 and water vapour. The closed flask, electric discharge was created at 800C.

(b) Amino acids were collected in the water at B. (c) He arrived at the conclusion that life came from pre-existing non-living organic molecules and that formation of life was preceded by chemical evolution.

232 Xam idea BiologyXII SECTIOND 30. (a) Streptococcus pneumoniae (b) Griffith observed two strains of the bacteriumone forming smooth shiny colonies (S-type) with capsule, while other forming rough colonies (R-type) without capsule. He injected live S-type cells into live mice and observed that they died due to pneumonia. He then injected live R-type cells into live mice and observed that they survived. When he injected heat-killed S-type cells into live mice, they survived and there were no symptoms of pnuemonia and when heat-killed S-type cells were mixed with live R-type cells and injected into mice, they died due to unexpected symptoms of pneumonia. He thus, concluded that heat-killed S-type bacteria caused a transformation of the R-type bacteria into S-type bacteria but he was not able to understand the cause of this bacterial transformation. (c) The transforming principle was nucleic acid, i.e., DNA. (d) It was proved by O. Avery, C. MacLeod and M. McCarty. OR (a) The DNA is associated with two types of proteinsbasic proteins (histone and protamine) and acidic non-histone chromosomal (NHC) proteins. The negatively charged DNA molecule wraps around the positively charged histone proteins to form a structure called nucleosome. The nucleosome core is made up of four types of histone proteinsH2A, H2B, H3 and H4 occurring in pairs. 200 bp of DNA helix wraps around the nucleosome by 1 turns, plugged by H1 histone protein. Repeating units of nucleosomes form the chromatin in nucleus, which is a thread-like structure. The chromatin is packed to form a solenoid structure of 30 nm diameter. Further supercoiling forms a looped structure called the chromatin fibre. (b)
S. No. (i) Euchromatin Regions of chromatin which are loosely packed during interphase are called euchromatin. When chromosomes are stained with Feulgen reaction (specific for DNA), these appear as lightly stained chromatin. Euchromatin contains active genes. They do not contain repetitive DNA sequences. Heterochromatin Regions of chromatin which are densely packed during cell division are called heterochromatin. When chromosomes are stained with Feulgen reaction, these appear as intensely stained chromatin. Heterochromatin contains inactive genes. They are enriched with highly repetitive tandemly arranged DNA sequences.

(ii)

(iii) (iv)

(c) The packaging of chromatin at higher level requires the presence of non-histone chromosomal protein.

Examination Papers 233

SETIII
SECTIONA 3. HardyWeinberg equation conveys genetic equilibrium, i.e., sum total of all allelic frequencies is 1. SECTIONB 9. (i) Haemophilia is sex-linked because it shows transmission from unaffected carrier female to some of the male progeny.

(ii) Haemophilia is caused by X-linked gene because the heterozygous female for haemophilia may transmit the disease to sons. 13. During pregnancy, placenta produces hormones like human chorionic gonadotropin, human placental lactogen and ovary produces relaxin. 16. (a) Restriction endonucleases cut DNA at specific points, hence the name molecular scissors.

(b) The convention for naming these enzymes is that the first letter to the name comes from the genes and the second two letters come from species of the prokaryotic cell from which they are isolated e.g., EcoRI comes from Escherichia coli RY. SECTIONC 21. (i) Histamines: These are chemicals which cause inflammatory responses. (ii) Interferons: These are glycoproteins which protect non-infected cells from further viral infection. (iii) B-cells: These produce proteins called antibodies in response to pathogens into the blood to fight with them. 25. Klinefelters syndrome. The karyotype is 44 + XXY. Symptoms are: (i) Sex of the individual is masculine but possess feminine characters. (ii) Gynaecomastia, i.e, development of breasts. (iii) Poor beard growth and often sterile. (iv) Feminine pitched voice. 26. Paul Ehrlich proposed the rivet popper hypothesis. This hypothesis states that in an airplane (ecosystem) all parts are joined together using thousands of rivet (species). If every passenger travelling in it starts popping a rivet to take home (causing a species to become extinct), it may not affect flight safety (proper functioning of the ecosystem) initially but as more and more rivets are removed, the plane becomes dangerously weak over a period of time. SECTIOND 28. (a) Wind pollinated flowers have light-weight, non-sticky, dry and winged pollens. The well-exposed stamens help in easy dispersal of pollen grains. The stigma is sticky, large and feathery to trap pollen grains floating in the air. Numerous flowers are packed together to form inflorescence.

234 Xam idea BiologyXII Insect pollinated flowers are large, sticky and brightly coloured with honey and nectar glands to attract insects. They are highly fragrant and the stigma is sticky. (b) Both Yucca plant and the moth cannot complete their life cycles without each other. The moth deposits its eggs in the locule of the ovary and the flower in turn, gets pollinated by the moth. The larvae of the moth come out of the eggs as the seeds start developing. OR (a) A develops into an embryo; B develops into endosperm. (b) Endosperm develops from primary endosperm nucleus (PEN). Endosperm develops first followed by an embryo. The PEN undergoes successive nuclear divisions to give rise to free nuclei and this stage of endosperm development is called free nuclear endosperm. Subsequently, cell wall is formed and endosperm becomes cellular. The water of tender coconut in the centre is free-nuclear endosperm and white kernel in the outer part is the multicellular endosperm. Non-albuminous seeds have no residual endosperm as it is completely consumed during embryo development (e.g., pea, groundnut). Albuminous seeds retain a part of endosperm as it is not completely used up during embryo development (e.g., wheat, maize, barley, castor, sunflower).

zzz

CBSE Examination Paper (Delhi 2012)

Time: 3 Hours Max. Marks: 70 General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. SectionA contains 8 questions of 1 mark each. SectionB is of 10 questions of 2 marks each. SectionC is of 9 questions of 3 marks each and SectionD is of 3 questions of 5 marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.

SETI
SECTIONA 1. Mention the unique flowering phenomenon exhibited by Strobilanthus kunthiana (neelakuranaji). 2. How does smoking tobacco in human lead to oxygen deficiency in their body? 3. A garden pea plant (A) produced inflated yellow pod, and another plant (B) of the same species produced constricted green pods. Identify the dominant traits. 4. Why is Eichhornia crassipes nicknamed as Terror of Bengal? 5. Write the location and function of the Sertoli cells in humans. 6. Name the following: (a) The semi-dwarf variety of wheat which is high-yielding and disease-resistant. (b) Any one inter-specific hybrid mammal. 7. Write the similarity between the wing of a butterfly and the wing of a bat. What do you infer from the above with reference to evolution? 8. Write what do phytophagous insects feed on. SECTIONB 9. Draw a neat labelled sketch of a replicating fork of DNA. 10. Where is sporopollenin in plants? State its significance with reference to its chemical nature. 11. (a) Highlight the role of thymus as a lymphoid organ. (b) Name the cells that are released from the above mentioned gland. Mention how they help in immunity.

236 Xam idea BiologyXII 12. Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology. 13. Why do clown fish and sea anemone pair up? What is this relationship called? 14. (a) State the difference between meiocyte and gamete with respect to chromosome number. (b) Why is a whiptail lizard referred to as parthenogenetic? 15. Name the plant source of the drug popularly called smack. How does it affect the body of the abuser? OR Why is Rhizobium categorised as a symbiotic bacterium? How does it act as a biofertiliser? 16. (a) State the role of DNA ligase in biotechnology. (b) What happens when Meloidegyne incognitia consumes cells with RNAi gene? 17. Some organisms suspend their metabolic activities to survive in unfavourable conditions. Explain with the help of any four examples. 18. (a) Name the protozoan parasite that causes amoebic dysentery in humans. (b) Mention two diagnostic symptoms of the disease. (c) How is this disease transmitted to others? SECTIONC 19. It is established that RNA is the first genetic material. Explain giving three reasons. OR (a) Name the enzyme responsible for the transcription of tRNA and the amino acid the initiator tRNA gets linked with. (b) Explain the role of initiator tRNA in initiation of protein synthesis. 20. State the theory of Biogenesis. How does Millers experiment support this theory? 21. Name the two different categories of microbes naturally occurring in sewage water. Explain their role in cleaning sewage water into usable water. 22. Write the function of each one of the following:

(a) (Oviducal) Fimbriae (b) Coleoptile (c) Oxytocin 23. Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.

Examination Papers 237 24. Study a part of the life cycle of malarial parasite given below. Answer the questions that follow:

(a) Mention the roles of A in the life cycle of the malarial parasite. (b) Name the event C and the organ where this event occurs. (c) Identify the organ B and name the cells being released from it. 25. Given below is the representation of amino acid composition of the relevant translated portion of b-chain of haemoglobin, related to the shape of human red blood cells.
Gene...CTC... ...GAG... mRNA...GAG...

Val 1

His 2

Leu 3

Thr

Pro

Glu 6

Glu 7

4 5 HbA Peptide

(a) Is this representation indicating a normal human or a sufferer from certain related genetic disease? Give reason in support of your answer. (b) What difference would be noticed in the phenotype of the normal and the sufferer related to this gene? (c) Who are likely to suffer more from the defect related to the gene represented the males, the females or both males and females equally? And why? 26. By the end of 2002 the public transport of Delhi switched over to a new fuel. Name the fuel. Why is this fuel considered better? Explain. 27. Draw a schematic sketch of pBR322 plasmid and label the following in it: (a) Any two restriction sites.

238 Xam idea BiologyXII (b) Ori and rop genes. (c) An antibiotic resistant gene. SECTIOND 28. Explain the carbon cycle with the help of a simplified model. OR Explain how does: (a) a primary succession start on a bare rock and reach a climax community? (b) the algal bloom eventually choke the waterbody in an industrial area? 29. The following is the illustration of the sequence of ovarian events (a i) in a human female.

(i) Identify the figure that illustrates ovulation and mention the stage of oogenesis it represents. (ii) Name the ovarian hormone and the pituitary hormone that have caused the above mentioned event. (iii) Explain the changes that occur in the uterus simultaneously in anticipation. (iv) Write the difference between c and h. (v) Draw a labelled sketch of the structure of a human ovum prior to fertilisation. OR How does the megaspore mother cell develop into 7-celled, 8 nucleate embryo sac in an angiosperm? Draw a labelled diagram of a mature embryo sac. 30. What is the inheritance pattern observed in the size of starch grains and seed shape of Pisum sativum? Workout the monohybrid cross showing the above traits. How does this pattern of inheritance deviate from that of Mendelian law of dominance? OR State the aim and describe Meselson and Stahls experiment.

SETII (Questions Uncommon to SETI)

SECTIONA 1. Cucurbits and papaya plants bear staminate and pistillate flowers. Mention the categories they are put under separately on the basis of the type of flowers they bear. 4. What is the interaction called between Cuscuta and shoe flower bush? 5. When do the oogenesis and the spermatogenesis initiate in human females and males respectively? 7. State the significance of the study of fossils in evolution.

Examination Papers 239 SECTIONB 13. Draw a schematic diagram of a part of double stranded dinucleotide DNA chain having all the four nitrogenous bases and showing the correct polarity. 14. Name the parasite that causes filariasis in human. Mention its two diagnostic symptoms. How is this disease transmitted to others? 15. Name the source of streptokinase. How does this bioreactor molecule function in our body? OR How do mycorrhizae act as biofertilisers? Explain. Name a genus of fungi that forms a mycorrhizal association with plants. SECTIONC 19. Write the function of each of the following: (a) Middle piece in human sperm. (b) Tapetum in anthers. (c) Luteinizing hormone in human males. 26. How does an algal bloom cause eutrophication of a water body? Name the weed that can grow in such a eutrophic lake. SECTIOND 28. (a) Draw a pyramid of numbers of a situation where a large population of insects feed upon a very big tree. The insects in turn, are eaten by small birds which in turn are fed upon by big birds. (b) Differentiate giving reason, between the pyramid of biomass of the above situation and the pyramid of numbers that you have drawn. OR (a) What are the two types of desirable approaches to conserve biodiversity? Explain with examples bringing out the difference between the two types. (b) What is the association between the bumble bee and its favourite orchid Ophrys? How would extinction or change of one would affect the other?

SETIII (Questions Uncommon to SETI & SETII)

SECTIONA 1. Mention the difference between spermiogenesis and spermiation. 3. What is an interaction called when an orchid grows on a mango plant? 4. Write the names of two semi-dwarf and high yielding rice varieties developed in India after 1966. 6. Mention the unique feature with respect to flowering and fruiting in bamboo species. 8. State the significance of biochemical similarities amongst diverse organism in evolution.

240 Xam idea BiologyXII SECTIONB 15. Mention the importance of Lactic acid bacteria to humans other than setting milk into curd. OR How do methanogens help in producing biogas? SECTIONC 19. (a) Construct a complete transcription unit with promoter and terminator on the basis of the hypothetical template strand given below:
A T G C A T G C A T A C

(b) Write the RNA strand transcribed from the above transcription unit along with its polarity. OR How are the structural genes inactivated in lac operon in E. coli? Explain. 20. Write the function of each of the following: (a) Seminal vesicle (b) Scutellum (c) Acrosome of human sperm. 25. (a) Why are the colourful polysterene and plastic packagings used for protecting the food, considered an environmental menace? (b) Write about the remedy found for the efficient use of plastic waste by Ahmed Khan of Bangalore. SECTIOND 30. Name the scientists who proved experimentally that DNA is the genetic material. Describe their experiment.

OR (a) List the three different allelic forms of gene I in humans. Explain the different phenotypic expressions, controlled by these three forms. (b) A woman with blood group A marries a man with blood group O. Discuss the possibilities of the inheritance of the blood groups in the following starting with yes or no for each: (i) They produce children with blood group A only. (ii) They produce children some with O blood group and some with A blood group.

Examination Papers 241

SOLUTIONS
SETI
SECTIONA 1. Strobilanthus kunthiana flowers once in 12 years. 2. Smoking increases the carbon monoxide (CO) content in the blood which has greater affinity to haemoglobin than oxygen. CO forms a stable bond with haemoglobin and does not allow binding of oxygen. Smoking also damages alveolar walls, which reduces respiratory surface (emphysema). 3. Inflated green pod is the dominant trait. 4. Eichhornia crassipes is an aquatic weed that grows abundantly and very fast in eutrophic water bodies and imbalances water ecosystem. It causes oxygen depletion leading to death of aquatic life (eutrophication). 5. Sertoli cells are present in seminiferous tubules. They provide nutrition to the germ cells or sperms. 6. (a) Kalyan Sona/Sonalika (b) Mule/Hinny/Liger/Tigon 7. Wings of a bird and a bat perform the same function of flying despite of their structural dissimilarity. This infers that they are analogous organs. 8. Phytophagus insects feed on plant sap and other parts of plant. SECTIONB 9.

10. Sporopollenin is present in the exine of pollen grains. It is the most resistant organic material in nature. It provides protection to the pollen/gamete/gametophyte from unfavourable conditions or chemicals (acids, enzymes and high temperature). 11. (a) Immature lymphocytes differentiate into mature lymphocytes and become antigensensitive in thymus. (b) T- lymphocytes are released from thymus. T-cells help B-cells to produce antibodies and provide cell-mediated immunity.

242 Xam idea BiologyXII 12. Stanley Cohen and Herbert Boyer in 1972 constructed the first recombinant DNA. They isolated the antibiotic resistance gene by cutting out a piece of DNA from the plasmid of a bacterium which was responsible for conferring antibiotic resistance. The cut piece of DNA was then linked with the plasmid DNA of Salmonella typhimurium and transferred to E. coli for transformation. 13. The clown fish gets protection from predators which stay away from stinging tentacles of anemone but anemone does not derive any benefit from the fish. This relationship is called commensalism. 14. (a) Meiocytes contain diploid sets of chromosomes whereas gametes contain haploid sets of chromosomes. (b) Whiptail lizard reproduces without fertilisation, i.e., an unfertilised egg develops into a new individual. Therefore, they are referred to as parthogenetic. 15. Plant source of smack is Papaver somniferum or poppy. Smack is a depressant and slows down body functions. OR Rhizobium is present in the root nodules of leguminous plants. Theirs is a symbiotic relationship in which the bacterium obtains food and shelter from the plant and the plant gets fixed nitrogen in return. These bacteria fix atmospheric nitrogen into organic forms, which is used by the plant as nutrient. 16. (a) DNA ligase joins the DNA with same sticky ends end to end./Link Okazaki fragments or discontinuously synthesised fragments./Link desired gene with plasmid to form recombinant DNA. (Any one) (b) The specific mRNA of the nematode is silenced and the parasite dies. 17. (i) Polar bearThey hibernate during winter to escape the cold weather. (ii) Snails/fishesThey go into aestivation during summer to avoid heat related problems and desiccation. (iii) Seeds of higher plants/spores of bacteria/fungiThey become dormant in unfavourable conditions and in case of Amoeba cyst formation takes place. (iv) Some species of zooplanktonThey undergo diapause. 18. (a) Entamoeba histolytica. (b) Symptoms: Constipation, abdominal pain, cramps, stools with excessive mucus and blood clots. (Any two) (c) It is transmitted by contaminated food or water. SECTIONC 19. The reasons in support are: (i) Processes like metabolism, translation and splicing evolved around RNA. (ii) RNA is reactive and catalyses reactions. (iii) In some viruses, RNA is the hereditary material. (iv) RNA is unstable and can be easily mutated leading to evolution. (Any three) OR (a) RNA polymerase III is responsible for transcription of tRNA and the initiator tRNA gets linked with the amino acid methionine.

Examination Papers 243 (b) The initiator tRNA, which is charged with amino acid methionine, reaches the smaller subunit of ribosome. Its anticodon UAC recognises the codon AUG on mRNA and binds by forming complementary base pairs. The large subunit of ribosome joins the smaller subunit and initiates translation. 20. The theory of biogenesis states that a living organism arises from another living organisms. Experimental evidence of chemical evolution/Millers experiment OO Experiment was performed by S.L. Miller and H.C. Urey in 1953. OO Experimental set-up: In a closed flask containing CH , H , NH and water vapour 4 2 3 at 800C, electric discharge was created. The conditions were similar to those in primitive atmosphere. OO Observations: After a week, they observed presence of amino acids and complex molecules like sugars, nitrogen bases, pigments and fats in the flask. Conclusions: (i) It provides experimental evidence for the theory of chemical origin. (ii) It showed that the first non-cellular form of life was created about 3 billion years ago. (iii) It showed that non-cellular biomolecules exist in the form of DNA, RNA, polysaccharides and protein. 21. Aerobic and anaerobic bacteria or fungi exist in sewage water. After the primary treatment of water, aerobic bacteria are added in aeration tanks. Growth of these bacteria reduces BOD as they consume organic matter. Anaerobic bacteria are added in anaerobic sludge digestors, where these digest the sludge and form biogas, etc. 22. (a) Collection of ovum released by ovary. (b) Protects the plumule of the monocot embryo. (c) Cause uterine contraction for parturition; promotes milk ejection. 23. Bt cotton has cryIAc/cryIIAb genes. These genes produce crystals of protoxin. When bollworm bites the cotton fruits, it consumes the toxic insecticidal protein. The alkaline pH in its gut activates the toxin. The activated toxin binds to mid-gut epithelial cells resulting in the lysis of cell leading to the death of the insect. 24. (a) AGametocytes of Plasmodium enter the mosquito when it bites a malarial patient and feed on blood. (b) CFertilisation. It occurs in the intestine of mosquito. (c) BSalivary gland of the female Anopheles mosquito. These release sporozoites of Plasmodium. 25. (a) This representation (HbA peptide) indicates a normal human because glutamic acid in the sixth position is not substituted by valine. (b) The sufferers RBC become elongated and sickle shaped whereas the normal person will have biconcave RBCs. (c) Both males and females are likely to suffer from the disease equally because this is not a sex-linked disease. It is an autosomal-linked recessive trait. 26. The fuel was CNG or compressed natural gas. CNG is considered better because of the following reasons: (i) CNG burns more efficiently unlike diesel or petrol.

244 Xam idea BiologyXII (ii) Very little of it is left unburnt. (iii) It cannot be adulterated. (iv) It is cheaper than petrol or diesel. 27.
d

rop

28.

E. coli cloning vector pBR322 showing restriction sites (HindIII, EcoRI, BamHI, SalI, PvuII, PstI, ClaI), ori and antibiotic resistance genes (ampR and tetR). rop codes for the proteins involved in the replication of the plasmid.

SECTIOND

Simplified model of carbon cycle in the biosphere

Examination Papers 245 (a) Primary succession on rocks

OO

OR

Lichens are the pioneer species on a bare area. OO The lichen secretes some acids to dissolve rock and help in weathering and soil formation. OO Later, some small bryophytes invade and hold the small amount of soil. OO The bryophytes are succeeded by herbs, shrubs and ultimately big trees. OO At last, a stable climax forest is formed. OO The xerophytic habitat gets converted into a mesophytic one. (b) Algal bloom Domestic sewage contains nutrients like nitrogen and phosphorus which favour the excessive growth of planktonic (free-floating) algae called an algal bloom. OO Algal bloom causes fish mortality and deterioration of water quality. OO Excessive growth of water hyacinth (Eichhornia crassipes), the worlds most problematic aquatic weed, also called Terror of Bengal, grows abundantly in eutrophic water bodies and imbalances water ecosystem. 29. (i) f; secondary oocyte. (ii) Estrogen and luteinizing hormone (LH) (iii) Endometrium proliferate (glands become cork-screw shaped) highly vascularised, high regeneration anticipating implantation of the fertilised ovum. (iv) cdeveloping follicle; hregressing corpus luteum. (v)
OO

Zona pellucida Plasma membrane

Ovum surrounded by few sperms

OR (i) Megaspore is the first cell of the female gametophyte. (ii) The megaspore increases in size and its nucleus divides mitotically into two nuclei which move apart to opposite poles. Thus, a 2-nucleate embryo sac is formed. (iii) The two daughter nuclei undergo another mitotic division giving rise to the 4-nucleate stage.

246 Xam idea BiologyXII (iv) The third mitotic division, which gives 8-nucleate embryo sac comprises of a micropylar and a chalazal end with four nuclei at each end. (v) One nuclei from each end proceeds towards the centre of the embryo sac and are called the polar nuclei. (vi) The three nuclei remaining at the micropylar region form the egg apparatus, without any cellular wall. (vii) In the egg apparatus, the middle cell is the largest and is called oosphere/egg/ovum, while other two naked cells adjoining the egg cell are called synergids. (viii) The three remaining nuclei at the chalazal end are surrounded by cytoplasm and cellular wall. These are called antipodal cells.

mature embryo sac 30. A single gene controls the size of the starch grains and seed shape of Pisum sativum.
Parents BB Big/Round Gametes B bb Small/Wrinkled b

A diagrammatic representation of the

F1 generation

Bb Intermediate/Round size On selfing

F2 generation

B B BB Big/Round Bb Intermediate size/Round

b Bb Intermediate size/Round bb Small/Wrinkled

Examination Papers 247 OR The aim of Meselson and Stahls experiment was to prove the mode of DNA replication is semi-conservative. OO Matthew Meselson and Franklin Stahl in 1958 performed experiments on E. coli to prove that DNA replication is semi-conservative. 15 OO They grew E. coli in a medium containing NH4Cl (in which 15N is the heavy isotope of nitrogen) for many generations. 15 OO As a result, N got incorporated into newly synthesised DNA. OO This heavy DNA can be differentiated from normal DNA by centrifugation in caesium chloride (CsCl) density gradient. 14 OO Then they transferred the cells into a medium with normal NH4Cl and took the samples at various definite time intervals as the cells multiplied. OO The extracted DNAs were centrifuged and measured to get their densities. 15 OO The DNA extracted from the culture after one generation of transfer from the N 14 medium to N medium (i.e., after 20 minutes; E. coli divides every 20 minutes) showed an intermediate hybrid density. OO The DNA extracted from culture after two generations (i.e., after 40 minutes) showed equal amounts of light DNA and hybrid DNA.

At 0 minutes

Meselson and Stahls experiment

After 20 minutes

After 40 minutes

SETII
SECTIONA 1. Cucurbit is a monoecious plant having staminate and pistillate flowers in the same plant. Papaya has staminate and pistillate flowers on separate plants and hence it is dioecious. 4. Parasitism. 5. Oogenesis in human females initiate at the foetal/embryonic stage. Spermatogenesis in human males starts at puberty. 7. Fossils represent extinct organisms./Show life forms restricted to certain geological time spans existing in the past./Show ancestry of present day organisms./Are connecting links between two groups of organisms. (Any one)

248 Xam idea BiologyXII SECTIONB 13.

DNA double helix 14. Wuchereria bancrofti/W. malayi causes filariasis in humans. Symptoms: Chronic inflation of the lymphatic vessels of the lower limbs and gross deformities of genital organs. This disease is transmitted through the bite of female mosquito vectors/Culex mosquito. 15. Source: Streptococcus. Streptokinase is a clot buster, i.e., it removes clot from the blood vessels of patients who had a heart attack.

OR Mycorrhizae is a symbiotic association of a fungus with roots of higher plants. The fungus absorbs phosphate from soil and passes it to the plant. It also provides resistance to root-borne pathogen and increase tolerance of plant to salinity and drought. This way they act as biofertilisers. Genus of fungi - Glomus. SECTIONC 19. (a) Provides energy for movement. (b) Provides nourishment to developing pollen. (c) Stimulates synthesis and secretion of androgens or male hormones. 26. Algal bloom in the lake or any other water body forms a scum. The scum depletes the oxygen in the water leading to foul smelling of the water body. The oxygen depletion affects the aquatic life adversely resulting in the death of fish and ultimately the eutrophic lake itself dies. Eicchornia crassipes/water hyacinth grows in a eutrophic lake.

Examination Papers 249 SECTIOND 28. (a) Large birds Small birds Insects Big tree Pyramid of number (b) Large birds Small birds Insects Big tree Pyramid of biomass OR (a) The two types of desirable approaches are: In situ conservation (On site conservation) and Ex situ conservation (Off-site conservation).
S. No. (i) In situ conservation It is conservation and protection of biodiversity in its natural habitat. Population is conserved in the surroundings where they have developed their distinctive features. e.g., national parks, biosphere reserves, wildlife sanctuaries, etc. Ex situ conservation It is conservation of selected threatened plant and animal species in places outside their natural habitat. Population is conserved under simulated conditions that closely resemble their natural habitats. e.g., botanical gardens, zoological parks, wildlife safari, gene banks, etc.

(ii)

(iii)

(b) Commensalism. If the female bees colour patterns change even slightly due to any reason during evolution, pollination success will be reduced unless the orchid flower co-evolves to maintain the resemblance of its petal to the female bee.

SETIII
SECTIONA 1. Spermiogenesis: It is the transformation of spermatids into spermatozoa or sperms. Spermiation: It is the release of sperms from seminiferous tubules. 3. Commensalism. 4. Jaya, Ratna. 6. Bamboo flowers once in its life time. It produces large number of fruits and dies.

250 Xam idea BiologyXII 8. Biochemical similarities indicate evolution from common or shared ancestry. SECTIONB 15. Lactic acid bacteria increase vitamin B12 absorption and check disease-causing microbes. OR Methanogens produce biogas by anaerobic decomposition of cellulosic material, i.e., excreta of cattle (dung). SECTIONC 19. (a)
3 Promoter 5 Terminater 3 A T G C A T G C A T A C 5

(b) Transcribed RNA

U A C G U A C G U A U G 5 3

OR The regulator gene produces repressor which is free. It binds to the operator region of the operon and prevents RNA polymerase from transcribing the structural gene. 20. (a) It is responsible for storage and transport of sperms. It provides secretions for motility and nourishment of sperms.

(b) It provides nourishment and protection to the developing embryo. (c) It helps the sperm to enter into the cytoplasm of the ovum through the zona pellucida and provides enzymes for fertilisation. 25. (a) The colourful polystyrene and plastic packagings are non-biodegradable and nonecofriendly. (b) The remedy was found by developing polyblend, which is a fine powder of recycled modified plastic. Polyblend is mixed with bitumen to lay roads. This enhanced the water repellent property of bitumen and enhanced the life of roads. SECTIOND 30. Alfred Hershey and Martha Chase proved that DNA is the genetic material.
OO

Hershey and Chase conducted experiments on bacteriophage to prove that DNA is the genetic material.

Examination Papers 251

Fig. 6.6 The HersheyChase experiment

OO

Procedure:

(i) Some bacteriophage virus were grown on a medium that contained radioactive phosphorus (32P) and some in another medium with radioactive sulphur (35S). (ii) Viruses grown in the presence of radioactive phosphorus (32P) contained radioactive DNA. (iii) Similar viruses grown in presence of radioactive sulphur (35S) contained radioactive protein. (iv) Both the radioactive virus types were allowed to infect E. coli separately. (v) Soon after infection, the bacterial cells were gently agitated in blender to remove viral coats from the bacteria. (vi) The culture was also centrifuged to separate the viral particle from the bacterial cell. OO Observations and Conclusions: (i) Only radioactive 32P was found to be associated with the bacterial cell, whereas radioactive 35S was only found in surrounding medium and not in the bacterial

252 Xam idea BiologyXII cell. (ii) This indicates that only DNA and not protein coat entered the bacterial cell. (iii) This proves that DNA is the genetic material which is passed from virus to bacteria and not protein. OR (a) The three different allelic forms are: IA, IB, IO/i. Co-dominance OO The alleles which are able to express themselves independently when present together are called co-dominant alleles and this biological phenomenon is called co-dominance. For example, ABO blood grouping in humans. A B OO ABO blood groups are controlled by gene I. Gene I has three alleles I , I and O I /i. A B OO I and I produce RBC surface antigens A and B, respectively, whereas i does not produce any antigen. A B OO I and I are the dominant alleles whereas i is the recessive allele. A B OO When I and I are present together, both express equally and produce the surface antigens A and B. (b) (i) Yes; when both the parent are homozygous.
Parents IAIA IOIO

Gametes

IA

IO

F1 generation

IAIO

(ii) Yes; when the woman is heterozygous.

Parents IAIO

IO

IOIO

Gametes

IA

IO

F1 generation

IAIO

IOIO

zzz

CBSE Examination Paper (All India 2012)

Time: 3 Hours General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. SectionA contains 8 questions of 1 mark each. SectionB is of 10 questions of 2 marks each. SectionC is of 9 questions of 3 marks each and SectionD is of 3 questions of 5 marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled. Max. Marks: 70

SETI
SECTIONA 1. Why is banana considered a good example of parthenocarpy? 2. State two different roles of spleen in the human body. 3. A garden pea plant produced axial white flowers. Another of the same species produced terminal violet flowers. Identify the dominant traits. 4. Why is it desirable to use unleaded petrol in vehicles fitted with catalytic converters? 5. Where is acrosome present in humans? Write its function. 6. Write the name of the following: (a) The most common species of bees suitable for apiculture. (b) An improved breed of chicken. 7. Comment on the similarity between the wing of a cockroach and the wing of a bird. What do you infer from the above, with reference to evolution? SECTIONB 9. (a) Draw a neat labelled diagram of a nucleosome. (b) Mention what enables histones to acquire a positive charge. 10. State one advantage and one disadvantage of cleistogamy. 11. (a) Where do the signals for parturition originate from in humans? (b) Why is it important to feed the newborn babies on colostrum?

8. Mention the role of cyanobacteria as a biofertiliser.

254 Xam idea BiologyXII 12. (a) A recombinant vector with a gene of interest inserted within the gene of a-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as insertional inactivation? 13. Explain brood parasitism with the help of an example. 14. Give reasons for the following: (a) The human testes are located outside the abdominal cavity. (b) Some organisms like honey-bees are called parthenogenetic animals. 15. Name the plant source of ganja. How does it affect the body of the abuser? OR Name the two special types of lymphocytes in humans. How do they differ in their roles in immune response? 16. (a) Mention the cause and the body system affected by ADA deficiency in humans. (b) Name the vector used for transferring ADA-DNA into the recipient cells in humans. Name the recipient cells. 17. How did Ahmed Khan, plastic sacks manufacturer from Bangalore, solve the everincreasing problem of accumulating plastic waste? 18. Name the bacterium that causes typhoid. Mention two diagnostic symptoms. How is this disease transmitted to others? SECTIONC 19. (a) Explain the phenomena of multiple allelism and co-dominance taking ABO blood group as an example. (b) What is the phenotype of the following: (i) IAi (ii) i i 20. How does industrial melanism support Darwins theory of Natural Selection? Explain. 21. (a) What is the programme called that is involved in improving success rate of production of desired hybrid and herd size of cattle? (b) Explain the method used for carrying this programme for cows. 22. Explain the function of each of the following: (a) Coleorhiza (b) Umbilical cord (c) Germ pores 23. How is the amplification of a gene sample of interest carried out using Polymerase Chain Reaction (PCR)?

24. Trace the life-cycle of malarial parasite in the human body when bitten by an infected female Anopheles. 25. List the salient features of double helix structure of DNA. OR How are the structural genes activated in the lac operon in E. coli?

Examination Papers 255 26. Alien species are highly invasive and are a threat to indigenous species. Substantiate this statement with any three examples. 27. (a) Tobacco plants are damaged severely when infested with Meloidegyne incognitia. Name and explain the strategy that is adopted to stop this infestation. (b) Name the vector used for introducing the nematode specific gene in tobacco plant. SECTIOND 28. (a) Taking one example each of habitat loss and fragmentation, explain how are the two responsible for biodiversity loss. (b) Explain two different ways of biodiversity conservation. OR (a) What depletes ozone in the stratosphere? How does this affect human life? (b) Explain biomagnification of DDT in an aquatic food chain. How does it affect the bird population? 29. The following is the illustration of the sequence of ovarian events a to i in a human female:

(a) Identify the figure that illustrates corpus luteum and name the pituitary hormone that influences its formation. (b) Specify the endocrine function of corpus luteum. How does it influence the uterus? Why is it essential? (c) What is the difference between d and e? (d) Draw a neat labelled sketch of Graafian follicle. OR (a) Why is fertilisation in an angiosperm referred to as double fertilisation? Mention the ploidy of the cells involved. (b) Draw a neat labelled sketch of L.S. of an endospermous monocot seed. 30. Describe Frederick Griffiths experiment on Streptococcus pneumoniae. Discuss the conclusion he arrived at. OR (a) Explain a monohybrid cross taking seed coat colour as a trait in Pisum sativum. Work out the cross up to F2 generation. (b) State the laws of inheritance that can be derived from such a cross. (c) How is the phenotypic ratio of F2 generation different in a dihybrid cross?

SETII (Questions Uncommon to SETI)

SECTIONA 1. How do the pollen grains of Vallisneria protect themselves? 2. Name the respective pattern of inheritance where F1 phenotype (a) does not resemble either of the two parents and is in between the two.

256 Xam idea BiologyXII (b) resembles only one of the two parents. 5. How is the entry of only one sperm and not many ensured into an ovum during fertilisation in humans? 7. State the significance of Coelacanth in evolution. SECTIONB 12. Name the source organism that possesses Taq polymerase. What is so special about the function of this enzyme? 15. Name the source of cyclosporin-A. How does this bioactive molecule function in our body? OR (a) Name the group of viruses responsible for causing AIDS in humans. Why are these viruses so named? (b) List any two ways of transmission of HIV infection in humans, other than sexual contact. 17. Name any two organisms that are responsible for ringworms in humans. Mention two diagnostic symptoms. Name the specific parts of the human body where these organisms thrive and explain why. SECTIONC 19. Differentiate between perisperm and endosperm giving one example of each. 25. (a) List any three ways of measuring population density of a habitat.

(b) Mention the essential information that can be obtained by studying the population density of an organism. SECTIOND 28. (a) Explain the significance of ecological pyramids with the help of an example. (b) Why are the pyramids referred to as upright or inverted? OR (a) Explain giving reasons why the tourists visiting Rohtang Pass or Mansarovar are advised to resume normal active life only after a few days of reaching there. (b) It is impossible to find small animals in the polar regions. Give reasons.

SETIII (Questions Uncommon to SetI and SetII)

SECTIONA 1. Identify the figure given below and the part labelled A.
A

Examination Papers 257 2. How do interferons protect us? 3. Name the interaction between a whale and the barnacles growing on its back. 7. In a dihybrid cross, when would the proportion of parental gene combinations be much higher than non-parental types, as experimentally shown by Morgan and his group? SECTIONB 12. Name the cells that act as HIV factory in humans when infected by HIV. Explain the events that occur in the infected cell.

15. Name the explain the two types of immune responses in humans. 16. How does the study of different parts of a flower help in identifying in wind as its pollinating agent? SECTIONC 22. Explain how do the following act as contraceptives: (a) CuT (b) Saheli 26. Name the explain the evolutionary concept represented in the illustration given below:

Mole

Ancestral stock

Rat

258 Xam idea BiologyXII OR (a) Why is it that the father never passes on the gene for haemophilia to his sons? Explain. (b) State the functions of the following in a prokaryote: (i) tRNA (ii) rRNA SECTIOND 29. (a) A garden pea plant bearing terminal, violet flowers, when crossed with another pea plant bearing axial, violet flowers, produced axial, violet flower and axial, white flowers in the ratio of 3 : 1. Work out the cross showing the genotypes of the parent pea plants and their progeny.

(b) Name and state the law that can be derived from this cross and not from a monohybrid cross. OR (a) Describe the process of synthesis of fully functional mRNA in a eukaryotic cell. (b) How is this process of mRNA synthesis different from that in prokaryotes?

Examination Papers 259

SOLUTIONS
SETI
SECTIONA 1. Because formation of fruit occurs without fertilisation, i.e., there is no formation of seeds. 2. Spleen is the secondary lymphoid organ that stores lymphocytes, it filters microbes and acts as a reservoir to store erythrocytes (Any two). 3. Axial, violet flower. 4. Lead in petrol inactivates the catalysts which convert harmful pollutants (CO, unburnt hydrocarbons, nitric oxide) to lesser harmful pollutants (CO2, H2O, N2). 5. On the sperm head.

It has enzymes to dissolve the follicles of ovum./It facilitates entry of sperm nucleus for fertilisation./It helps the sperm enter into the cytoplasm of the ovum.(Any one) 6. (a) Apis indica/Apis mellifera/Apis dorsata 7. They are similar in function. Thus we infer that these organs are analogous which has resulted in convergent evolution. 8. It is a biological organism that fixes atmospheric nitrogen. SECTIONB 9. (a) (b) Leghorn/Rhode island red/Minorcha

Nucleosome

(b) Basic amino acid residues of lysines and arginines.

260 Xam idea BiologyXII 10. Advantage: Self-pollination is assured/Seed production is assured. Disadvantage: Least variations observed/Leads to inbreeding depression. 11. (a) From the fully developed foetus/placenta/foetal ejection reflex (Any one) (b) Contains antibodies (IgA), to (passively) immunise the baby. 12. (a) Bacteria is grown in a medium with chromogenic substrate, blue coloured colonies show no recombinations and colonies with no blue colour show presence of recombinants. (b) Gene for the enzyme is inactivated by insertion. 13. Koel is a parasitic bird (which has lost the instinct to make its own nest to lay eggs) has evolved the technique of laying eggs in the nest of a crow. Its eggs bear resemblances to those of crow. 14. (a) To maintain the temperature (2-2.5C) lower than the normal internal body temperature, is essential for spermatogenesis.

(b) They (drones/males) develop from unfertilised eggs. 15. Cannabis sativa/hemp plant. It damages cardio-vascular system. OR B lymphocytes, T lymphocytes. B-cells produce pathogen specific antibodies/humoral immune response. T-cells help the B-cells to produce antibodies/are responsible for cell mediated immunity. 16. (a) The cause is the defective gene not producing ADA. The immune system is affected. (b) A retroviral vector is used, recipient cells are lymphocytes. 17. He collected plastic wastes and recycled them. He powdered plastic to form polyblend, which is blended with bitumen. Polyblend was used in road laying, which increased road life by a factor of three, making it more durable. 18. Salmonella typhi. Constipation, stomach pain, headache, weakness, loss of appetite, high fever. (Any two) The disease is transmitted through contaminated food/water. SECTIONC 19. (a) Multiple allelism is the phenomenon of occurrence of a gene in more than two allelic forms on the same locus. In ABO blood group in humans one gene I has three alleles IA, IB and IO/i. Co-dominance is the phenomena in which both alleles express themselves when present together. We inherit any two alleles for the blood group. When the genotype is IAIB the individual has AB blood group since both IA and IB equally influence the formation of antigens A and B. (b) (i) IA A blood group. (ii) ii O blood group.

Examination Papers 261 20. Before industrial revolution the environment was unpolluted. The lichens on the barks of trees were pale. The white-winged moths could easily camouflage, while the darkwinged were spotted out by the birds for food. Hence, they could not survive. After industrial revolution the lichens became dark (due to soot deposit). This favoured the dark-winged moths while the white-winged were picked by birds. The population of the former which was naturally selected increased.

21. (a) Multiple ovulation embryo transfer method/MOET. (b) Procedure (i) A cow is administered hormones with FSH-like activity to induce follicular maturation and super-ovulation. (ii) The cow produces 68 eggs instead of one egg produced normally. (iii) It is now, either mated with an elite bull or artificial insemination is carried out. (iv) When the fertilised eggs attain 832 cells stage, they are non-surgically removed and transferred to a surrogate mother. (v) The genetic mother can now be again super-ovulated. 22. (a) Coleorhiza: Protects the radical of (monocot) embryo. (b) Umbilical cord: Transports nutrients and respiratory gases and metabolic wastes to and from mother and foetus. (c) Germ pores: Allow germination of pollen grain/formation of pollen tubes. 23. PCR is carried out in the following three steps: The double-stranded DNA is denatured by applying high temperature of 95C for 15 seconds. Each separated single stranded strand now acts as template for DNA synthesis. (b) Annealing
OO OO

(a) Denaturation

Two sets of primers are added which anneal to the 3 end of each separated strand.

Primers act as initiators of replication. (c) Extension

OO OO

DNA polymerase extends the primers by adding nucleotides complementary to the template provided in the reaction. A thermostable DNA polymerase (Taq polymerase) is used in the reaction which can tolerate the high temperature of the reaction. All these steps are repeated many times to obtain several copies of desired DNA.

OO

OO

262 Xam idea BiologyXII

Polymerase chain reaction (PCR)

24. Sporozoite of Plasmodium gets into human blood through the bite of female Anopheles mosquito. Sporozoites reproduce asexually in liver cells, then they get into red blood cells, where they reproduce asexually and infect more blood cells. After a while they change into gametocytes, which are picked up by the mosquitoes and the entire cycle occurs again. 25. Salient Features of Double Helical DNA: (i) DNA is made up of two polynucleotide chains, where the backbone is made up of sugar and phosphate groups and the nitrogenous bases project towards the centre. (ii) There is complementary base pairing between the two strands of DNA. (iii) The two strands are coiled in right-handed fashion and are anti-parallel in orientation. One chain has a 53 polarity while the other has 35 polarity. (iv) The diameter of the strand is always constant due to pairing of purine and pyrimidine, i.e., adenine is complementary to thymine while guanine is complementary to cytosine. (v) The distance between the base pairs in a helix is 0.34 nm and a complete turn contains approximately ten base pairs. The pitch of the helix is 3.4 nm and the two strands are right-handed coiled.

Examination Papers 263 OR Lactose acts as the inducer that binds with repressor protein and frees the operator gene. RNA polymerase freely moves over the structural genes, transcribing lac mRNA, which in turn produces the enzymes responsible for the digestion of lactose. 26. (i) Nile perch introduced into lake Victoria in East Africa led to the extinction of Cichild fish.

(ii) Parthenium/Lantana/Eichhornia are invasive plants and pose a threat to indigenous species. (iii) Introduction of African catfish (Clarias gariepinus) to aquaculture is a threat to Indian catfishes. 27. (a) Gene expression can be controlled by using RNA molecule and this technology is called RNA interference or RNAi or gene silencing. During this process nematode specific gene is introduced into host plant (using Agrobacterium) which produce dsRNA. This specific mRNA of the nematode silenced and parasite dies. SECTIOND 28. (a) Habitat loss and fragmentation Destruction of habitat is the primary cause of extinction of species. The tropical rainforests initially covered 14 per cent of the land surface of earth, but now cover only 6 per cent of land area. OO The Amazon rainforest (called the lungs of the planet) is being cut and cleared for cultivation of soya beans and for conversion into grasslands for raising beef cattle. OO When large-sized habitats are broken or fragmented due to human settlements, building of roads, digging of canals, etc., the population of animals requiring large territories and some animals with migratory habitats declines. (b) The two different ways of biodiversity conservation are: Ex situ and in situ conservation.
OO OO

(b) Agrobacterium tumifaciens.

In ex situ conservation threatened organism are taken out from the natural habitat and placed in special setting with care and protected, e.g., zoological park/botanical garden/wild safari. In in situ conservation threatened organisms are conserved in their natural habitat, e.g., national parks/biosphere reserves. OR (a) Chlorofluorocarbons (CFCs). UV(B) damages DNA causing mutation, skin cancer, inflammation of cornea, cataract, aging of skin, snow blindness. (b) If DDT leaches from the agricultural field, it gets into the water body (the concentration is 0.0003 ppm) and enters the food chain: zooplanktons (0.04 ppm) small fish (0.05 ppm) large fish (2 ppm) any fish eating bird (5 ppm).

264 Xam idea BiologyXII Concentration of DDT increases along the food chain, reaching a high level in the top carnivore bird. DDT concentration disturbs Ca++ metabolism, egg shells become thin, premature breaking resulting in decline in bird population. 29. (a) Corpus luteum is illustrated by g and the hormone influencing its formation is luteininsing hormone (LH). (b) Produces the hormone progesterone, causes proliferation of the endometrium which gets highly vascularised. It is essential for the implantation of the fertilised ovum and maintains the same during pregnancy. (c) d is the developing tertiary follicle. e is the Graafian follicle. (d)

OR (a) Fertilisation of haploid egg cell by one haploid male gamete to form diploid zygote is called syngamy. Fertilisation of two (diploid) polar nuclei by the other haploid male gamete to form triploid primary endosperm nucleus is called triple fusion. (b)

Examination Papers 265 30. Transforming Principle

OO

Frederick Griffith (1928) conducted experiments with Streptococcus pneumoniae (bacterium causing pneumonia). He observed two strains of this bacteriumone forming smooth shiny colonies (S-type) with capsule, while other forming rough colonies (R-type) without capsule. When live S-type cells were injected into mice, they died due to pneumonia. When live R-type cells were injected into mice, they survived. When heat-killed S-type cells were injected into mice, they survived and there were no symptoms of pnuemonia. When heat-killed S-type cells were mixed with live R-type cells and injected into mice, they died due to unexpected symptoms of pneumonia. He concluded that heat-killed S-type bacteria caused a transformation of the R-type bacteria into S-type bacteria but he was not able to understand the cause of this bacterial transformation. OR

OO

OO OO OO

OO

OO

(a)

Parents

YY Yellow

yy Green

F1 generation

Yy Yellow Selfed

F2 generation
Y

Y YY Yellow

y Yy Yellow

y Yy Yellow yy Green

F2 Phenotypic ratio = 3 : 1 F2 Genotypic ratio = 1 : 2 : 1 (b) Law of Dominance: In a contrasting pair of factors one member of the pair dominates (dominant) the other (recessive). Law of Segregation: Factors or allele of pair segment from each other such that gamete receives only one of the two factors. (c) Phenotypic ratio of F2 in monohybrid cross is 3 : 1 whereas in a dihybrid cross the phenotypic ratio is 9 : 3 : 3 : 1.

266 Xam idea BiologyXII

SETII
SECTIONA 1. They have mucilaginous covering to prevent them from getting wet. 2. (a) Incomplete dominance. (b) Dominance (law). 5. On the sperm head. Has enzymes to dissolve the follicles of ovum/facilitate entry of sperm nucleus for fertilisation/help the sperm enter into the cytoplasm of the ovum. 7. It is an ancestor of amphibians. SECTIONB 12. Thermus aquaticus. The enzyme can tolerate high temperature/ is thermostable. It does not get denatured during PCR at high temperature. 15. Trichoderma polysporum. It acts as an immuno-suppresant and is used in organ transplant patient. OR (a) Retrovirus. These are named so because they (have RNA genome) have reverse transcriptase enzyme which carries on the processes RNA DNA RNA. (b) Infected blood transfusion/sharing syringes/needles, children born to HIV mothers. (Any two) 17. Microsporum/Trichophyton/Epideromophyton. Symptoms: Dry/scaly lesion on skin/nails/scalp/intense itching (Any two). These thrive in body groin/between toes, thrive better in heat/moisture/perspiration. SECTIONC 19.
S.No. (i) (ii) (iii) Perisperm It is persistent nucellus. It is diploid. Example: black pepper, beet. Endosperm It is the nutritive tissue for embryo. It is triploid. Example: maize, rice, wheat, castor.

25. (a) By physical counting, percent cover or total biomass, from relative density, counting pug marks, counting faecal pellets. (Any three) (b) Status of habitat, whether competition for survival exists or not, whether population is increasing or declining, natality, mortality, emigration, immigration. (Any three) SECTIOND 28. (a) Ecological pyramid expresses the relationship between the organisms at different trophic levels with reference to their number, energy and biomass. e.g. Pyramid of energy

Examination Papers 267

Top Carnivore Secondary Consumer Primary Consumer

10 J/Least energy

100 J/Lesser energy

1000 J/Less energy

Primary Producer

10000 J/High energy

(b) Pyramid of energy is always upright as only 10% energy is transferred from one trophic level to the next. Inverted pyramid shows less number/biomass of producers when compared to primary consumers. OR (a) Initially the person suffers from altitude sickness/nausea, fatigue and heart palpitation because of low oxygen availability and low atmospheric pressure. Gradually the body increases RBC production, decreasing binding capacity of Hb and increases the breathing rate to get acclimatised. (b) Small birds have larger surface area relative to their volume, so they lose heat much faster spend more energy to generate body heat.

SETIII
SECTIONA 1. The figure is of blastula/blastocyst. ATrophoblast. 2. Interferons protect non-infected cells from further viral infections, by creating cytokine barriers.

3. Commensalism 7. When the genes are linked SECTIONB 12. Macrophages/Helper T-cells act as HIV factory. The virus enters macrophages or helper T-cells where RNA genome of the virus replicates to form viral DNA with the help of the enzyme reverse transcriptase. The viral DNA then gets incorporated into host cells DNA and directs infected cells to produce virus particles.

268 Xam idea BiologyXII 15. The two types of immunity are active immunity and passive immunity. Active immunity: Immunity developed in the host body due to production of antibodies in response to antigens. Passive immunity: When ready-made antibodies are directly given to protect the body against foreign agents. OR The two types of immunity are humoral immunity and cell-mediated immunity. Humoral immunity: Immune respones given by antibodies found in the blood. Cell-mediated immunity: T-lymphocytes mediate this immunity. OR The two types of immunity are primary immunity and secondary immunity. Primary immunity: When our body encounters a pathogen for the first time produces primary response. Secondary immunity: Subsequent encounter with the same pathogen generates highly intensified secondary response. 16. Wind pollinated flowers have light and non sticky pollen grains, well exposed stamens, large and often feathery stigma, single ovule in each ovary, numerous flowers packed into an inflorescence.(Any four) SECTIONC 22. (a) Cu ions released suppress sperm motility, lowers the fertilising capacity of sperms. (b) Inhibit ovulation, implantation, as well as alter the quality of cervical mucus to prevent or retard the entry of sperms. 26. The illustration represents adaptive radiation.
OO

Placental animals in Australia A variety of placental mammals have evolved which appear similar to a corresponding marsupial. When more than one adaptive radiation appear to have occurred in an isolated geographical area (representing different habitats), and two or more groups of unrelated animals come to resemble each other for similar mode of life or habitat, it is called convergent evolution. For example, Australian marsupials, placental mammals. OR (a) Haemophilia is a sex-linked recessive disease and the defective gene is present on X chromosome only and not on Y chromosome. Father never passes X chromosome to the son. Therefore, father can never pass the defective gene to son. (b) (i) tRNA reads the genetic codes, carries amino acids to the site of protein synthesis and act as an adaptor molecule. (ii) rRNA plays structural and catalytic role during translation.

OO

Examination Papers 269 SECTIOND 30. (a)

Parents Vvaa Violet, Terminal VvAA Violet, Axial

Gametes

Va

va

VA

vA

F1 generation

VVAa Violet, Axial

VvAa Violet, Axial 3

VvAa Violet, Axial :

vvAa White axial 1

(b) Law of Independent Assortment.

The law states that when two pairs of traits are combined in a hybrid, segregation of one pair of characters is independent of the other pair of character. OR (a) Post-transcriptional modifications
OO

The primary transcripts are non-functional, containing both the coding region, exon, and non-coding region, intron, in RNA and are called heterogenous RNA or hnRNA. The hnRNA undergoes two additional processes called capping and tailing. In capping, an unusual nucleotide, methyl guanosine triphosphate, is added to the 5-end of hnRNA. In tailing, adenylate residues (about 200300) are added at 3-end in a template independent manner.

OO OO

OO

Now the hnRNA undergoes a process where the introns are removed and exons are joined to form mRNA by the process called splicing. (b) In prokaryotes, there is a single DNA-dependent RNA polymerase that catalyses transcription of all types of RNA in bacteria. In bacteria, mRNA does not require any processing as it does not have any introns.
OO

zzz

CBSE Examination Paper (Foreign 2012)

Time: 3 Hours Max. Marks: 70 General Instructions: (i) All questions are compulsory. (ii) The question paper consists of four sections A, B, C and D. SectionA contains 8 questions of 1 mark each. SectionB is of 10 questions of 2 marks each. SectionC is of 9 questions of 3 marks each and SectionD is of 3 questions of 5 marks each. (iii) There is no overall choice. However, an internal choice has been provided in one question of 2 marks, one question of 3 marks and all the three questions of 5 marks weightage. A student has to attempt only one of the alternatives in such questions. (iv) Wherever necessary, the diagrams drawn should be neat and properly labelled.

SETI
SECTIONA 1. How is it possible in Oxalis and Viola plants to produce assured seed-sets even in the absence of pollinators? 2. Why do pollen grains of some flowers trigger sneezing in some people? 3. A garden pea plant produced round green seeds. Another of the same species produced wrinkled yellow seeds. Identify the dominant traits. 4. Why is an earthworm called a detritivore? 5. Mention the location and the function of Leydig cells in humans. 6. Why is the South Indian sugarcane preferred by agriculturalists? 7. Comment on the similarity between the flippers of dolphins and penguins, with reference to evolution. 8. Mention the effect of global warming on the geographical distribution of stenothermals like amphibians. SECTIONB 9. Draw a labelled schematic diagram of a transcription unit. 10. Write the cellular contents carried by the pollen tube. How does the pollen tube gain its entry into the embryo sac? 11. Describe the role of lymph nodes in providing immunity. 12. How can the following be made possible for biotechnology experiments? (a) Isolation of DNA from bacterial cell. (b) Reintroduction of the recombinant DNA into a bacterial cell.

Examination Papers 271 13. Refrigerants are considered to be a necessity in modern living, but are said to be responsible for ozone holes detected in Antarctica. Justify. 14. Name the product of fertilisation that forms the kernel of cocount. How does the kernel differ from coconut water? 15. Name the plant source of cocaine. How does it affect the human body? OR How does culturing Spirulina solve the food problems of the growing human population? 16. (a) How does cryIAc gene express itself in its host?

(b) State the role of this gene in controlling the infestation of bollworm. 17. Why are small birds like humming birds not found in polar regions? Explain. 18. Name the parasite that causes ascariasis. Mention two diagnostic symptoms of the disease. How is this disease transmitted to others? SECTIONC 19. Why is DNA considered a better hereditary material than RNA? OR How is hnRNA processed to form mRNA? 20. Evolution is a change in gene frequencies in a population in response to changes in the environment in a time scale of years and not centuries. Justify this statement with reference to DDT. How does the theory of Hugo de Vries support this? 21. (a) Name the Indian scientist whose efforts brought green revolution in India. (b) Mention the steps that are essentially carried out in developing a new genetic variety of crop under plant breeding programme. 22. Mention the function of each of the following: (a) tassels of corn-cob.

(b) mitochondria in sperm. (c) tapetum in the microsporangium. 23. Explain the synthesis of genetically engineered human insulin. 24. How does AIDS virus enter the human body? Describe its life cycle. Why does this infection shatter the immunity of the victim? 25. (a) Explain sex determination in humans. (b) How do human males with XXY abnormality suffer? 26. List the reasons that account for greater biological diversity in the tropics. 27. How are the DNA fragments separated and isolated for DNA fingerprinting? Explain. SECTIOND 28. (a) Name the green house gases. How do they affect life on earth? (b) Describe the cases of eutrophication of a lake.

272 Xam idea BiologyXII OR (a) How does primary succession start in water and lead to the climax community? Explain. (b) Explain giving reasons why thermal power plants are not considered eco-friendly. 29. (a) How does microspore mother cell develop into mature pollen gain in angiosperms? (b) Describe the structure of a mature pollen grain and draw a labelled diagram of its two-celled stage. OR Study the illustration given and answer the questions that follow:
b

(i) Identify a. (ii) Name and state the function of c. (iii) Identify d. (iv) Explain the role of hormones in the formation and release of a. (v) Draw a diagram of b separately and label the parts: that help its entry into a; that carry genetic material; that help in its movement. 30. (a) Snapdragon shows incomplete dominance for flower colour. Work out a cross and explain the phenomenon. How is this inheritance different from Mendelian pattern of inheritance? Explain. (b) Draw the structure of a tRNA charged with methionine. OR Describe the HersheyChase experiment. Write the conclusion they arrived at after the experiment.

Examination Papers 273

SETII (Questions Uncommon to SETI)

SECTIONA 1. Papaver and Michelia both have multicarpellary ovaries. How do they differ from each other? 5. Name the category of the disease Rheumatoid arthritis. 6. Name the interaction between sea anemone and the hermit crab that grows on it. 8. Write the possible genotypes Mendel got when he crossed F1 tall pea plants with a dwarf pea plant. SECTIONB 10. (a) Mention the similarity between autogamy and geitonogamy. (b) How does geitonogamy differ from xenogamy? 11. How has recombinant technology helped in large scale production of vaccines? Explain giving one example. 15. Name the source of statin and state its action on the human body. OR How is it possible to recover healthy banana plants from a diseased but desirable quality banana plant? Explain. SECTIONC 19. State the conditions when genetic code is said to be (i) degenerate, (ii) unambigous and specific, (iii) universal. OR Explain the process of translation in a bacterium. 24. Name the phenomenon that leads to situations like XO abnormality in humans. How do humans with XO abnormality suffer? Explain. SECTIOND 30. Answer the following questions based on Messelson and Stahls experiment: (a) Why did the scientists use 15NH4Cl and 14NH4Cl as sources of nitrogen in the culture medium for growing E. coli? (b) Name the molecule(s) that 15N got incorporated into. (c) How did they distinguish between 15N labelled molecules from 14N ones? (d) Mention the significance of taking the E. coli samples at definite time intervals for observations. (e) Write the observations made by them from the samples taken at the end of 20 minutes and 40 minutes respectively. (f) Write the conclusion drawn by them at the end of their experiment. OR

274 Xam idea BiologyXII (a) How does HardyWeinberg equation explain genetic equilibrium? (b) Describe how does this equilibrium get disturbed which may lead to founder effect.

SETIII (Questions Uncommon to SetI and SetII)

SECTIONA 1. Banana produces fruits but is propagated only by vegetative means. Why is it so? 2. Why, in a test cross, did Mendel cross a tall pea pant with a dwarf pea plant only? 4. Mention the role of pioneer species in primary succession on rocks. 6. Mention the useful as well as the harmful drug obtained from the latex of Poppy plant. SECTIONB 9. Name the different types of cells providing cellular barriers responsible for innate immunity in humans. 12. Draw a schematic diagram of lac operon in its switched off position. Label the following: (i) The structural genes (ii) Repressor bound to its correct position (iii) Promoter gene (iv) Regulatory gene. 15. How are biofortified maize and wheat considered nutritionally improved? OR Why is an antibody represented as H2L2? SECTIONC 19. Explain the process of transcription in a bacterium. OR Forensic department was given three blood samples. Write the steps of the procedure carried to get the DNA fingerprinting done for the above samples. 24. Explain how does trisomy of 21st chromosome occur in humans. List any four characteristic features in an individual suffering from it. SECTIOND 30. (a) Explain the process of natural selection that leads to speciation. (b) List the three different ways in which this process operates in nature. Explain any one of the processes. OR (a) Four children with four different blood groups are born to parents where the mother has blood group A and the father has blood group B. Work out the cross to show the genotypes of the parents and all four children. (b) Explain the contribution of Alfred Sturtevant in Chromosome mapping.

Examination Papers 275

SOLUTIONS
SETI
SECTIONA 1. By presence of cleistogamous flowers. 2. They result in an allergic reaction. 3. Round yellow seed is the dominant trait. 4. This is because earthworm breaks down detritus into smaller particles. 5. Leydig cells are present in seminiferous tubules. They synthesise and secrete androgens. 6. South Indian sugarcane has thicker stem and higher sugar content. 7. Similar habitat resulted in selection of similar adaptive features in different groups of organisms. 8. Due to global warming, stenothermals would either migrate or die due to change in the temperature. SECTIONB 9.

Schematic structure of a transcription unit 10. Pollen tube carries two male gametes. Pollen tube, after reaching the ovary, enters the ovule through the micropyle and then enters one of the synergids through the filiform apparatus which guides the entry of pollen tube.

11. Lymph nodes trap microorganisms or other antigens. These trapped antigens activate lymphocytes present in the lymph and cause an immune response. 12. (a) Gel electrophoresis

(b) Microinjection/gene gun 13. The widely used refrigerants are CFCs or chlorofluorocarbons. CFCs discharged in the lower part of atmosphere move upwards to the stratosphere. Here, the UV rays act on them and release chlorine atoms. These free chlorine atoms react with ozone to release molecular oxygen. Chlorine atoms are not consumed in this reaction and hence, these continuously degrade ozone and have resulted in ozone hole. 14. Endosperm. The coconut water is free-nuclear endosperm whereas kernel is cellular endosperm. 15. Plant source of cocaine is Erythroxylum coca. It has a potent stimulating action on central nervous system, producing a sense of euphoria and increased energy. Excessive dosage of cocaine causes hallucinations.

276 Xam idea BiologyXII OR Spirulina can be grown easily on materials like waste water from potato processing plants, straw, molasses, animal manure and even sewage, to produce large quantities. It serves as a food rich in protein, minerals, fats, carbohydrate and vitamins, being environment friendly. 16. (a) cryIAc gene codes for a toxic insecticidal protein that controls the cotton bollworms. (b) This gene codes for a toxin that becomes active when ingested by the insect. The activated toxin binds to the surface of mid-gut epithelial cells thus creating pores which causes cell swelling and lysis, further leading to death of the insects. 17. Small animals have larger surface area relative to their volume, they tend to lose body heat very fast when it is cold outside; therefore they have to expend much energy to generate body heat through metabolism. This is the reason why polar regions are not a suitable habitat for tiny humming birds. 18. Ascariasis is caused by Ascaris lumbricoides, commonly called roundworm. Symptoms: Abdominal pain, indigestion, internal bleeding, muscular pain, fever, anaemia (Write any two). It is transmitted through contaminated fruits, vegetables and water. SECTIONC 19. DNA is considered a better hereditary material than RNA because of the following reasons: (i) It is able to generate its replica (replication). (ii) It is chemically and structurally stable. (iii) It provides the scope for slow changes (mutation) that are required for evolution. (iv) It expresses itself in the form of Mendelian characters. OR The hnRNA undergoes the following processes to form mRNA: (i) Capping: Addition of methyl guanosine triphosphate at 5'-end. (ii) Tailing: Addition of 200-300 adenylate residues at 3'-end. (iii) Splicing: Removal of introns and joining of exons. 20. When DDT was used for the first time, maximum mosquitoes died but few survived due to variation in a population. These mosquitoes show resistance to DDT and survived to reproduce successfully in the presence of DDT and gradually such mosquito population become DDT resistant within a time span of few years. According to Hugo de Vries, evolution is caused by sudden large differences in the population and not minor variations. 21. (a) M.S. Swaminathan (b) The steps are: (i) Collection of variability. (ii) Evaluation and selection of parents. (iii) Cross hybridisation among the selected parents. (iv) Selection and testing of superior recombinants. (v) Testing, release and commercialisation of new cultivars.

Examination Papers 277 22. (a) These are the stigma and style which wave in the wind to trap pollen grains. (b) Provide energy for the movement of sperm tail. (c) Provides nourishment to the developing pollen grains. 23. Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly, in 1983. It prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli for production. The A and B chains produced were separated, extracted and combined by creating disulfide bonds to form human insulin. 24. Life cycle of HIV
OO OO

After getting into the body of a person, the virus enters the macrophages.

Here, RNA is replicated to form viral DNA by enzyme reverse transcriptase. OO The viral DNA now gets incorporated into the host cells DNA and directs the infected cells to produce viruses. OO The macrophages continue to produce virus particles. OO The virus particles enter helper T-lymphocytes (T H cells) in the blood, where they continue to replicate and produce viral progenies. OO Thus, the number of helper T-lymphocytes progressively decreases in the body of the infected persons. OO With the decrease in number of T-cells, the immunity also decreases. The person is unable to produce any immune response even against common bacteria like Mycobacterium, parasites like Toxoplasma, viruses and fungi. 25. (a) By the process of spermatogenesis, males produce two types of sperms50% carrying X-chromosome and 50% carrying Y-chromosome. However, females produce only one type of ovum carrying X-chromosome. If the sperm carrying X-chromosome fertilises the ovum, the zygote will develop into a female (XX) and if the sperm carrying Y-chromosome fertilises the ovum, the zygote develops into a male (XY).

(b) The XXY individual suffers from Klinefelters syndrome. 26. The three hypotheses to explain species richness in tropics are: (i) The constant environment in tropics promotes niche specialisation and increased species diversity. (ii) There is longer exposure to solar radiation in the tropical regions that contributes directly to higher productivity and indirectly to greater species diversity.

278 Xam idea BiologyXII (iii) There occurred no glaciation in tropical region and it remained undisturbed. Thus organisms living in tropics continued to flourish and evolved more species diversity. 27. Separation and Isolation of DNA Fragments (Gel Electrophoresis) OO Gel electrophoresis is a technique for separating DNA fragments based on their size. OO Firstly, the sample DNA is cut into fragments by restriction endonucleases. OO The DNA fragments being negatively charged can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. OO Commonly used matrix is agarose, which is a natural linear polymer of D-galactose and 3, 6-anhydro-L-galactose which is extracted from sea weeds. OO The DNA fragments separate-out (resolve) according to their size because of the sieving property of agarose gel. Hence, the smaller the fragment size, the farther it will move. OO The separated DNA fragments are visualised after staining the DNA with ethidium bromide followed by exposure to UV radiation. OO The DNA fragments are seen as orange coloured bands. OO The separated bands of DNA are cut out and extracted from the gel piece. This step is called elution. OO The purified DNA fragments are used to form recombinant DNA which can be joined with cloning vectors. SECTIOND 28. (a) Carbon dioxide, methane, nitrous oxide and chlorofluorocarbons are the greenhouse gases. They absorb the radiations reflected by the earths surface and eat it up. (b) Eutrophication
OO OO OO OO OO

OO OO

It is defined as the natural aging of a lake by biological enrichment of its water. Water in a young lake is cold and clear to support life. With time, it is enriched with nutrients by streams draining into it. This encourages growth of aquatic lifeplant and animal life. Organic remains deposit at the bottom of the lake and with time makes the water warmer. Eventually, floating plants develop in the lake, finally converting into land. The accelerated aging of lakes due to sewage and agricultural and industrial wastes is called cultural or accelerated eutrophication. OR

(a) Primary succession in water

OO OO OO OO OO

The pioneer species are phytoplanktons. The phytoplanktons are replaced by free-floating angiosperms. Then, rooted angiosperms invade sedges, grasses and finally the trees. At last, a stable climax forest is formed. An aquatic habitat is converted into mesic habitat.

Examination Papers 279

Diagrammatic representation of primary succession

(b) Thermal power plants release particulate and gaseous pollutants in the environment. Inhalation of these pollutants can cause breathing or respiratory symptoms, irritation, inflammation, damage to lungs and even premature death. 29. (a) Microsporogenesis The process of formation of microspore from a pollen mother cell by meiosis is called microsporogenesis. OO Each cell of the sporogenous tissue in a microsporangium acts as a potential pollen mother cell (PMC) or microspore mother cell. OO PMC undergoes meiotic divisions to form cluster of four cells called microspore tetrad. OO On maturity, the anther dehydrates and the microspores separate from each other to form pollen grains. (b) Pollen grains are generally spherical in structure.
OO OO

They possess two prominent layered wallsouter exine and inner intine.

Generative cell

Mature pollen grain

280 Xam idea BiologyXII

OO

OO OO

The exine is a hard layer made of sporopollenin which is one of the most resistant organic material present in nature. The inner thin layer of intine is made up of cellulose and pectin. The exine has an aperture where sporopollenin is absent, called germ pore. OR

(i) aovum. (ii) czona pellucida. It protects ovum and regulates interaction between ovum and sperms during fertilisation. (iii) dcells of corona radiata. (iv) Follicular stimulating hormone (FSH) stimulate follicular development. Luteinizing hormone (LH) ruptures Graafian follicle and thereby release ovum. (v)

Structure of a sperm

30. (a) In incomplete dominance the genes of an allelomorphic pair are not expressed as dominant and recessive but express themselves partially when present together in a hybrid and is an intermediate between the two genes. As a result an intermediate character is obtained. e .g., Two types of flowers occur snapdragon. The red flower colour is due to gene RR, white flower colour is due to gene rr and pink flower colour is due to gene Rr. In Mendelian inheritance, only one allele is dominant which expresses itself whereas the other allele which does not express itself in presence of the dominant gene is called the recessive allele.

Examination Papers 281

Phenotypic ratio Genotypic ratio

(b)

Monohybrid cross in Snapdragon, where one allele is incompletely dominant over the other allele
Met

C A

OR Hershey and Chase conducted experiments on bacteriophage to prove that DNA is the genetic material. OO Procedure: (i) Some bacteriophage virus were grown on a medium that contained radioactive phosphorus (32P) and some in another medium with radioactive sulphur (35S). (ii) Viruses grown in the presence of radioactive phosphorus (32P) contained radioactive DNA.

282 Xam idea BiologyXII (iii) Similar viruses grown in presence of radioactive sulphur (35S) contained radioactive protein. (iv) Both the radioactive virus types were allowed to infect E. coli separately. (v) Soon after infection, the bacterial cells were gently agitated in blender to remove viral coats from the bacteria. (vi) The culture was also centrifuged to separate the viral particle from the bacterial cell. OO Observations and Conclusions: (i) Only radioactive 32P was found to be associated with the bacterial cell, whereas radioactive 35S was only found in surrounding medium and not in the bacterial cell. (ii) This indicates that only DNA and not protein coat entered the bacterial cell. (iii) This proves that DNA is the genetic material which is passed from virus to bacteria and not protein.

SETII
SECTIONA 1. Papaver has syncarpous gynoecium whereas Michelia has apocarpous gynoecium. 5. Auto-immune disease. 6. Commensalism. 8. Possible genotypes: Tt and tt. SECTIONB 10. (a) In both cases pollen grains come from the same plant. (b) In geitonogamy pollen grains are transferred from the anther to the stigma of another flower of the same plant whereas in xenogamy pollen grains are transferred from the anther to the stigma of a different flower. 11. Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly, in 1983. It prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli for production. The A and B chains produced were separated, extracted and combined by creating disulfide bonds to form human insulin. 15. Statin is produced by the yeast Monascus purpureus. It acts as a blood-cholesterol lowering agent by competitively inhibiting the enzyme responsible for synthesis of cholesterol. OR Healthy bananas can be obtained by tissue culture technique. The meristem (apical and axillary) is free from virus. Hence, it is removed and grown in vitro to obtain healthy banana.

Examination Papers 283 SECTIONC 19. (i) DegenerateWhen some amino acids are coded by more than one amino acids. (ii) Unambiguous and specificWhen one codon codes for only one amino acid. (iii) The actual structure of tRNA looks like inverted L. OR Translation OO Translation is the process of synthesis of protein from mRNA with the help of ribosome. OO A translational unit in mRNA from 5' 3' comprises of a start codon, region coding for a polypeptide, a stop codon and untranslated regions (UTRs) at both 5'-end and 3'-end for efficient process. OO There are three stages of protein synthesis: (i) Initiation Assembly of ribosome on mRNA. Activation of amino acids and its delivery to tRNA. (ii) Elongation
OO OO

Repeated cycle of amino acid delivery. Peptide bond formation and movement along the mRNA called translocation. (iii) Termination
OO OO OO The release of a polypeptide chain. 24. Absence of one X chromosome leads to XO abnormality. These are sterile female with rudimentary ovaries. They have shield-shaped thorax, webbed neck, poor development of breasts, short stature, small uterus and puffy fingers.

SECTIOND 30. (a) N is the heavy isotope of nitrogen and it can be separated from 14N based on the difference in their densities. (b) 15N was incorporated into newly synthesised DNA. (c) The two molecules were distinguished by cesium chloride centrifugation in which these two separated based on their densities. (d) E. coli culture is taken at equal intervals to know the progress of the experiment. (e) After 20 minutes the culture had an intermediate density and after 40 minutes, the culture had equal amounts of hybrid DNA and the light DNA. (f) They concluded that DNA replicates semi-conservatively. OR (a) Hardy-Weinberg equation is p2 + 2pq + q2 = 1. This means that the sum total of all the allelic frequencies is 1. In a diploid, p2 means that the probability an allele AA with a frequency of p appear on both the chromosomes of a diploid individual will be p2. Similarly of allele aa is q2, and of Aa is 2pq. (b) When migration of a section of population to another place and population occurs, gene frequencies change in the original as well as in the new population. New genes/
15

284 Xam idea BiologyXII alleles are added to the new population and these are lost from the old population. Sometimes the change in allele frequency is so different in the new sample of population that they become a different species. The original drifted population becomes founders and the effect is called founder effect.

SETIII
SECTIONA 1. 2. 4. 6. Banana does not produce seeds, therefore fertilisation is not possible. To determine the genotype of F2 generation. The pioneer species invade a bare area and pave way for other species. Useful drugmorphine. Harmful drugheroin. SECTIONB 9. Polymorphonuclear leukocytes, monocytes, natural killer cells and macrophages. 12.

(i) z, y and a are structural genes. (ii) p is the promoter gene. (iii) i is the regulatory gene. 15. Biofortified maize had twice the amount of amino acids, lysine and tryptophan, compared to existing hybrids and the wheat variety had increased protein content. OR Each antibody molecule has four polypeptide chains. The two smaller chains are called light chains while the two longer chains are called heavy chains. Therefore an antibody is represented as H2L2. SECTIONC 19. Transcription in Prokaryotes OO In prokaryotes, the structural gene is polycistronic and continuous. OO In bacteria, the transcription of all the three types of RNA (mRNA, tRNA and rRNA) is catalysed by single DNA-dependent enzyme, called the RNA polymerase. OO In E. coli bacterium, the RNA polymerase has co-factors , , a, a and w along with s (sigma) factor, to catalyse the process.

Examination Papers 285

OO OO

OO

OO

OO

The transcription is completed in three steps: initiation, elongation and termination. Initiation: s (sigma) factor recognises the start signal and promotor region on DNA which then along with RNA polymerase binds to the promoter to initiate transcription. Elongation: The RNA polymerase after initiation of RNA transcription loses the s factor but continues the polymerisation of ribonucleotides to form RNA. Termination: Once the RNA polymerase reaches the termination region of DNA, the RNA polymerase is separated from DNARNA hybrid, as a result nascent RNA separates. This process is called termination which is facilitated by a termination factor r (rho). In prokaryotes, mRNA does not require any processing, so both transcription and translation occur in the cytosol. It can be said that transcription and translation are coupled together. OR

Methodology and Technique (i) DNA is isolated and extracted from the cell or tissue by centrifugation. (ii) By the process of polymerase chain reaction (PCR), many copies are produced. This step is called amplification. (iii) DNA is cut into small fragments by treating with restriction endonucleases. (iv) DNA fragments are separated by agarose gel electrophoresis. (v) The separated DNA fragments are visualised under ultraviolet radiation after applying suitable dye. (vi) The DNA is transferred from electrophoresis plate to nitrocellulose or nylon membrane sheet. This is called Southern blotting. (vii) Probes are now added which bind to specific nucleotide sequences that are complementary to them. This is called hybridisation. (viii) The hybridised DNA fragments are detected by autoradiography. They are observed as dark bands on X-ray film. 24. Downs syndrome Cause: Additional copy of chromosome number 21 or trisomy of chromosome 21. Symptoms: (i) Short statured with small round head. (ii) Partially open mouth with protruding furrowed tongue. (iii) Palm is broad with characteristic palm crease. (iv) Slow mental development. SECTIOND 30. (a) Charles Darwin, based on his observations during a sea voyage around the world in the ship H.M.S. Beagle. He observed varying degrees of similarities between existing life forms and those that existed millions of years ago. He gave the theory of ntural selection. According to it those populations which are better fit (reproductively fit) in an environment will be selected by nature and will survive more. Adaptability is inherited and fitness is the end result of ability to adapt and get selected by nature.

286 Xam idea BiologyXII (b) Natural selection is based on following factual observations: Limited natural resources. Stable population size except seasonal fluctuation. OO Varying characteristics of members of a population. OO Most of the variations are inherited. QQ Example of natural selection. Industrial melanism:
OO OO OO

OO

OO

OO

 n England, before industrialisation, white-winged moths were more in number I than dark-winged moths. But after industrialisation dark-winged moths became more in number than white-winged moths. This is because during industrialisation, the tree trunks covered by white lichens became dark due to dust and coal particles. As a result, white-winged moths could be easily picked up by predators from the dark background and dark-winged moths survived. OR
Parents Blood group Gamets IA I A IO A IO IB IB IO B IO

(a)

F1 generation IB

IA IA I B Blood group AB IA IO Blood group A

IO IBIO Blood group B IO IO Blood group O

IO

(b) Alfred Sturtevant used the frequency of recombination between gene pairs on the same chromosome as a measure of the distance between genes and mapped their position on the chromosome.

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Important Diagrams

Important Diagrams 289

Fig. 1 A diagrammatic representation of L.S. of a Flower

Fig. 2 T.S. of an ovary

290 Xam idea BiologyXII

Synergids

Fig. 3 Anatropous ovule

Zona pellucida

Fig. 4 Fertilisation of ovum

Important Diagrams 291

Fig. 5 Schematic structure of a transcription unit

Fig. 6 Simplified model of carbon cycle in the biosphere

292 Xam idea BiologyXII

rop

Fig. 7 E. coli cloning vector pBR322 showing restriction sites (HindIII, EcoRI, BamHI, SalI, PvuII, PstI, ClaI), ori and antibiotic resistance genes (ampR and tetR). rop codes for the proteins involved in the replication of the plasmid.

Fig. 8 Structure of some seeds

Important Diagrams 293

Fig. 9 Growth of pollen tube into pistil

Fig. 10 Human sperm

294 Xam idea BiologyXII

Fig. 11 Deoxyribonucleic acid (DNA)

Fig. 12 Nucleosome

Important Diagrams 295

Antigen binding sites

Variable region on heavy chain

Light chain Variable region on light chain Disulfide bridges Constant region on light chain Constant region on heavy chain

Heavy chain

Fig. 13 Structure of antibody

Fig. 14 Seminiferous tubule

296 Xam idea BiologyXII

Fig. 15 Primary endosperm nucleus (PEN)

Fig. 16 Replication fork