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Methodology 2

Two sets of 6 test tubes were prepared, one with diastase solution and one with distilled water. The test tubes were placed in water baths at different temperatures for 10 minutes then cooled. Starch was added to each test tube and iodine solution was used to test for remaining starch. Observations were recorded to see the effect of temperature on diastase's ability to break down starch.

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27 views1 page

Methodology 2

Two sets of 6 test tubes were prepared, one with diastase solution and one with distilled water. The test tubes were placed in water baths at different temperatures for 10 minutes then cooled. Starch was added to each test tube and iodine solution was used to test for remaining starch. Observations were recorded to see the effect of temperature on diastase's ability to break down starch.

Uploaded by

lynaAPPLES
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Methodology

Procedures
1.Prepare two sets of 6 test tubes . 2.Fill the first 6 test tubes with 5ml of diastase solution and the second set of test tubes with 5ml of distilled water. 3.Put one of each test tubes into 6 different water baths of ;10degrees,40 degrees, 60 degrees,80 degrees and 100 degrees.Measure the temperatures using thermometer. 4.Leave the test tubes for 10 miutes. 5.After 10 mins,take out each test tubes and let them cool at room temperature. 6.Drop 5ml of starch into each test tubes. 7.Add iodine solution to a few drops of each of the solutions to test availability of starch.Then, leave it for 30 minutes. 8.Record the observation.

Materials
5ml of diastase, 5ml of distilled water, iodine solution, 5ml of starch.

Apparatus
2 set of 6 test tubes,stopwatch,dropper,water bath, thermometer,test tube rack.

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