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    Column Chromatography (CC)

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    liska ramdanawati
    Column chromatography is a technique used to purify synthetic or natural products by separating compounds through differential intermolecular forces between the components and the mobile and stationary phases. Silica gel is commonly used as the polar stationary phase, with polar components adhering more strongly and eluting later than less polar components. Compounds are separated as they travel through the column at different rates depending on their interactions with the mobile and stationary phases.

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    Column Chromatography (CC)

    Uploaded by

    liska ramdanawati
    11 views12 pages
    Column chromatography is a technique used to purify synthetic or natural products by separating compounds through differential intermolecular forces between the components and the mobile and stationary phases. Silica gel is commonly used as the polar stationary phase, with polar components adhering more strongly and eluting later than less polar components. Compounds are separated as they travel through the column at different rates depending on their interactions with the mobile and stationary phases.

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    3122008 Cc

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    3122008 Cc

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    Column chromatography is a technique used to purify synthetic or natural products by separating compounds through differential intermolecular forces between the components and the mobile and stationary phases. Silica gel is commonly used as the polar stationary phase, with polar components adhering more strongly and eluting later than less polar components. Compounds are separated as they travel through the column at different rates depending on their interactions with the mobile and stationary phases.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    11 views12 pages

    Column Chromatography (CC)

    Uploaded by

    liska ramdanawati
    Column chromatography is a technique used to purify synthetic or natural products by separating compounds through differential intermolecular forces between the components and the mobile and stationary phases. Silica gel is commonly used as the polar stationary phase, with polar components adhering more strongly and eluting later than less polar components. Compounds are separated as they travel through the column at different rates depending on their interactions with the mobile and stationary phases.

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    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
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    COLUMN CHROMATOGRAPHY (CC)

    TLC - Optimizing for column chromatography

    Optimum: 0.2 < Rf < 0.5

    COLUMN CHROMATOGRAPHY Column chromatography (CC) is an extremely valuable technique for purification of synthetic or natural products. Compounds are separated by CC through the same mechanism as TLC; through differential intermolecular forces between the components of the mixture with the mobile phase, and between the components with the stationary phase. A variety of adsorbents can be used as the stationary phase; silica gel (which is very polar) is most commonly used in organic chemistry.

    COLUMN CHROMATOGRAPHY

    Polar components (b) adsorb more strongly to the polar silica and elute after the less polar components (a), which move more quickly with the non-polar (relative to silica) solvent.

    COLUMN CHROMATOGRAPHY

    Skoog and Leary: Principals of Instrumental Analysis, 4th ed. Suanders, 1992

    FLASH COLUMN CHROMATOGRAPHY

    FLASH COLUMN CHROMATOGRAPHY

    Build column Apply sample Elute Collect Fractions Analyze by TLC Combine pure fractions

    FLASH COLUMN CHROMATOGRAPHY

    FCC - OPTIMIZATION

    Measure of column efficiency

    FCC Sample vs Column Size

    TLC vs High Performance Liquid Chromatography (HPLC)

    TLC vs High Performance Liquid Chromatography (HPLC)

    http://www.labhut.com/education/flash/introduction07.php

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