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Sem3 P4 Syllabus

The document outlines the key topics covered in four units of a biochemistry techniques course. Unit I discusses various chromatographic techniques including paper, thin layer, gas, and reversed phase chromatography. Unit II covers ion exchange, gel exclusion, affinity, and HPLC chromatography. Unit III focuses on spectroscopic methods like IR, ESR, NMR, and mass spectrometry. Unit IV examines electrophoretic techniques such as paper, gel, and capillary electrophoresis along with their applications in separating peptides, proteins, and nucleic acids.

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60 views2 pages

Sem3 P4 Syllabus

The document outlines the key topics covered in four units of a biochemistry techniques course. Unit I discusses various chromatographic techniques including paper, thin layer, gas, and reversed phase chromatography. Unit II covers ion exchange, gel exclusion, affinity, and HPLC chromatography. Unit III focuses on spectroscopic methods like IR, ESR, NMR, and mass spectrometry. Unit IV examines electrophoretic techniques such as paper, gel, and capillary electrophoresis along with their applications in separating peptides, proteins, and nucleic acids.

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vsiddharthareddy
Copyright
© Attribution Non-Commercial (BY-NC)
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P3: BIOCHEMICAL TECHNIQUES / SEM-III / SIDDY UNIT-I: CHROMATOGARPHIC METHODS. Principles & applications of techniques based on partitioning.

tioning. Counter current distribution. Paper chromatography (ascending, descending, two dimensional, circular). Thin layer chromatography- types of adsorbent, detection methods. Reversed phase chromatography. Gas chromatography. Methods of detection-flame ionization. ECD.

APPLICATIONS IN SEPERATION & PURIFICATION OF CHOS, A.AS, PEPTIDES, LIPIDS & NUCLEIC ACIDS. UNIT II: CHROMATOGRAPHIC METHODS II Ion- exchange chromatography- principle, types of exchangers & exchange capacity. Elution methods (stepwise, linear & isocratic elution). Applications of ion exchange chromatography. Gel exclusion chromatography, types of matrices & applications. Methodology & application of affinity chromatography. HPLC & reverse phase techniques. Chromatography of chiral compounds.

APPLICATIONS IN SEPERATION & PURIFICATION OF A.AS, PEPTIDES, PROTEINS, ENZYMES, NUCLEIC ACIDS, VITAMINS & PHARMACEUTICALS. UNIT III: SPECTROSCOPIC METHODS II Basic principles & applications of IR, ESR, NMR & Mass spectroscopy. Principles & applications of biophysical techniques such as ORD, CD & X-ray diffraction in relation to conformation analysis of bio molecules (outlines only). Instrumental aspects of design & operation.

UNIT IV: ELETROPHORETIC TECHNIQUES

Concept of electrophoresis- moving boundary & zonal electrophoresis, (paper ,cellulose acetate, gel). Principles & applications of native &SDS, PAGE, IEF, 2D. Capillary, High voltage, Agarose gel electrophoresis, Pulse field gel electrophoresis. Zymography. Blotting techniques- Southern, Northern & Western Blotting. APPLICATIONS IN SEPERATION AND ANALYSIS OF PEPTIDES, PROTEINS & NUCLEIC ACIDS

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