Biochemistry

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Biochemistry Fundamental Principles of Biochemistry and Biotechnology

(Grundlagen der Biochemie einschl. Biotechnologie) Lecture: 2 hours per week (winter semester) ECTS credits: 2 Topics : Peptides: structure, functions Enzymes: mechanisms, kinetics Glucose metabolism, citrate cycle, fermentation, oxidative phosphorylation Lipid metabolism, fatty acid degradation and biosynthesis, steroid biosynthesis Amino acid metabolism, transamination, urea cycle, nucleobase syntheses DNA and RNA structure, protein biosynthesis Literature : Lffler/Petrides Biochemie und Pathobiochemie Voet/Voet: Biochemie Stryer: Biochemistry no assessment

Biochemical Techniques

(Biochemische Untersuchungsmethoden) Lecture: 2 hours per week (summer semester) ECTS credits: 2 Topics : "Biochemical" buffers, buffer additives, sample preparation, isolation of proteins and nucleic acids, absorption spectroscopy (biochemical and clinical applications), analysis of amino acids, peptides and proteins, electrophoresis, radiochemistry, immunochemistry, enzymatic analysis (theory and clinical applications), chromatography, ligand binding, fluorescence spectroscopy (applications, e.g. intrinsic fluorescence of proteins, fura-assay), centrifugation Literature : Lottspeich S, Zorbas H (Hrsg) (1998) Bioanalytik, Spektrum Akademischer Verlag, Heidelberg, Berlin Wilson K, Walker J (Eds) (1997) Practical Biochemistry, Cambridge University Press, Cambridge Freifelder D (1982) Physical Biochemistry, Applications to Biochemistry and Molecular Biology, 2nd edn, WH Freeman and Company,New York

no assessment

Biochemical Methods and Clinical Chemistry


(Biochemische Untersuchungsmethoden einschl. klinische Chemie) Practical Course: 13 hours per week (summer semester) ECTS credits: 11 Topics : Enzymes: kinetics, activities, isolation DNA isolation, purification, characterization, and analysis Radiochemical analysis, hemoglobin determination, urine analyses Peptide analysis, electrophoresis, metabolism, enzyme induction Literature : Cooper: Biochemische Arbeitsmethoden Pindur/Pindur: Klinische Chemie Cooper: The Tools of Biochemistry Method of assessment : written examination

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