Biochemistry
Biochemistry
Biochemistry
(Grundlagen der Biochemie einschl. Biotechnologie) Lecture: 2 hours per week (winter semester) ECTS credits: 2 Topics : Peptides: structure, functions Enzymes: mechanisms, kinetics Glucose metabolism, citrate cycle, fermentation, oxidative phosphorylation Lipid metabolism, fatty acid degradation and biosynthesis, steroid biosynthesis Amino acid metabolism, transamination, urea cycle, nucleobase syntheses DNA and RNA structure, protein biosynthesis Literature : Lffler/Petrides Biochemie und Pathobiochemie Voet/Voet: Biochemie Stryer: Biochemistry no assessment
Biochemical Techniques
(Biochemische Untersuchungsmethoden) Lecture: 2 hours per week (summer semester) ECTS credits: 2 Topics : "Biochemical" buffers, buffer additives, sample preparation, isolation of proteins and nucleic acids, absorption spectroscopy (biochemical and clinical applications), analysis of amino acids, peptides and proteins, electrophoresis, radiochemistry, immunochemistry, enzymatic analysis (theory and clinical applications), chromatography, ligand binding, fluorescence spectroscopy (applications, e.g. intrinsic fluorescence of proteins, fura-assay), centrifugation Literature : Lottspeich S, Zorbas H (Hrsg) (1998) Bioanalytik, Spektrum Akademischer Verlag, Heidelberg, Berlin Wilson K, Walker J (Eds) (1997) Practical Biochemistry, Cambridge University Press, Cambridge Freifelder D (1982) Physical Biochemistry, Applications to Biochemistry and Molecular Biology, 2nd edn, WH Freeman and Company,New York
no assessment