Introduction Wastewater

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Introduction: Biochemical oxygen demand or B.O.D.

is the amount of dissolved oxygen needed by aerobic biological organisms in a body of water to break down organic material present in a given water sample at certain temperature over a specific time period. The term also refers to a chemical procedure for determining this amount. This is not a precise quantitative test, although it is widely used as an indication of the organic quality of water. The BOD value is most commonly expressed in milligrams of oxygen consumed per litre of sample during 5 days of incubation at 20 C and is often used as a robust surrogate of the degree of organic pollution of water. BOD can be used as a gauge of the effectiveness of wastewater treatment plants. The BOD5 test Dilution method To ensure that all other conditions are equal a very small amount of microorganism seed is added to each sample being tested. This seed is typically generated by diluting organisms with buffered dilution water. The BOD test is carried out by diluting the sample with oxygen saturated dilution water, inoculating it with a fixed aliquot of seed, measuring the dissolved oxygen (DO) and then sealing the sample to prevent further oxygen dissolving in. The sample is kept at 20 C in the dark to prevent photosynthesis (and thereby the addition of oxygen) for five days, and the dissolved oxygen is measured again. The difference between the final DO and initial DO is the BOD. The loss of dissolved oxygen in the sample, once corrections have been made for the degree of dilution, is called the BOD5. For measurement of carbonaceous BOD (cBOD), a nitrification inhibitor is added after the dilution water has been added to the sample. The inhibitor hinders the oxidation of ammonia nitrogen. BOD can be calculated by: Undiluted: Initial DO - Final DO = BOD Diluted: ((Initial DO - Final DO)- BOD of Seed) x Dilution Factor BOD is similar in function to chemical oxygen demand (COD), in that both measure the amount of organic compounds in water. However, COD is less specific, since it measures everything that can be chemically oxidized, rather than just levels of biologically active organic matter.

Objective:

The objective of this experiment is to detemine the Biochemical Oxygen Demand (BOD) for sample from Taman Jati by calculating the Dissolved Oxygen (DO) of the sample.

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