Effect of photocatalysis on the physicochemical properties of liquid digestate

Antibiotic residues pose a risk to the agricultural application of liquid digestate. In our previous study, photocatalysis was employed to degrade the antibiotics in liquid digestate and observed that the removal efficiency of TC, OTC, and CTC was up to 94.99%, 88.92%, and 95.52%, respectively, at the optimal experimental level, demonstrating the feasibility of this technology. In this study, the liquid digestate after photocatalysis was analyzed to evaluate the effect of photocatalysis on the nutrients, phytotoxicity, and bacterial community of liquid digestate. The results showed that photocatalysis had little effect on the major nutrients TN, TP, and TK in liquid digestate. However, photocatalysis could cause an increase in tryptophan substances as well as soluble microbial by-products and a decrease in humic acid substances in the liquid digestate. The toxicity of liquid digestate after photocatalysis exhibited an increasing trend followed by a decreasing trend, and the liquid digestate after photocatalysis for 2 h had a promoting effect on seed germination and root growth. The richness, diversity, and evenness of bacterial communities in liquid digestate were decreased as a result of photocatalysis. The dominant species in the liquid digestate was dramatically changed by photocatalysis, and the antibiotic concentration also had a major effect on the dominant species in the liquid digestate after photocatalysis. After photocatalysis for 2 h, the dominant species in the liquid digestate changed from Firmicutes to Proteobacteria.