Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities

Carbohydr Polym. 2020 May 15:236:116075. doi: 10.1016/j.carbpol.2020.116075. Epub 2020 Feb 26.

Abstract

One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8-73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223-444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80-4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.

Keywords: Antibacterial activity; Chitosan; Clove essential oil; Nanoencapsulation; Nanoparticles.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Antioxidants / pharmacology*
  • Chitosan / chemistry*
  • Clove Oil / pharmacology*
  • Drug Carriers / chemistry*
  • Escherichia coli / drug effects
  • Listeria monocytogenes / drug effects
  • Microbial Sensitivity Tests
  • Nanoparticles / chemistry*
  • Oils, Volatile / pharmacology
  • Particle Size
  • Salmonella typhimurium / drug effects
  • Staphylococcus aureus / drug effects
  • Syzygium / chemistry

Substances

  • Anti-Bacterial Agents
  • Antioxidants
  • Clove Oil
  • Drug Carriers
  • Oils, Volatile
  • Chitosan