Development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid VP2 protein carrying immunoreactive epitope of porcine circovirus type 2

Vaccine. 2008 Apr 16;26(17):2119-26. doi: 10.1016/j.vaccine.2008.02.037. Epub 2008 Mar 13.

Abstract

Virus-like particles (VLPs) are non-replicative vectors for delivery of heterologous epitopes and induction of immune responses. In this study, a self-assembled porcine parvovirus (PPV) VP2 capsid protein [PPV:VLP-(PCV2)] carrying immunoreactive epitopes, residues 165-200 from the Porcine circovirus 2 (PCV2) virus nucleoprotein was constructed. Immunogenicity study was carried out with hybrid VLPs derived from HEK-293 cells infected with recombinant adenovirus vectors. To our knowledge, this study presents the first demonstration that hybrid non-replicative PPV VLPs carrying PCV2 immunoreactive epitopes can induce stronger antibody responses against PCV2 than recombinant adenovirus of PCV2 ORF2, in the absence of any adjuvant. The hybrid VLPs [PPV:VLP-(PCV2)] might be a promising candidate vaccine for better prevention of the diseases associated with PCV2 as well as with co-infection by PCV2 and PPV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / immunology*
  • Capsid Proteins / genetics
  • Capsid Proteins / immunology*
  • Cell Line
  • Circovirus / genetics
  • Circovirus / immunology*
  • DNA, Recombinant / genetics
  • DNA, Recombinant / immunology*
  • Epitopes / genetics
  • Epitopes / immunology
  • Genetic Vectors / chemistry*
  • Mice
  • Parvovirus, Porcine / genetics
  • Parvovirus, Porcine / immunology*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology

Substances

  • Antigens, Viral
  • Capsid Proteins
  • DNA, Recombinant
  • Epitopes
  • Recombinant Proteins
  • Viral Vaccines