Abstract
The Saccharomyces cerevisiae gene CDC6, whose protein product is required for DNA replication, is transcribed only in late G1 and S phases. We have discovered a critical reason why CDC6 expression is regulated in this fashion. Constitutive CDC6 transcription greatly delayed the initiation of M phase without effecting the G1-S transition or growth rate. This occurred in both fission and budding yeasts. The CDC6-induced M phase delay was dependent on the wee1/mik1 mitotic inhibitor kinases and was greatly accentuated in strains defective for the cdc25/MIH1 mitotic inducer phosphatases, indicating that CDC6 indirectly inhibits activation of the p34cdc2/CDC28 M phase kinase. Thus CDC6 appears to have an important and perhaps unique dual role in S phase, it is first required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Cell Cycle / drug effects
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Cell Cycle / genetics*
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Cell Cycle Proteins*
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Cell Division
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Cell Nucleus / physiology*
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Cell Nucleus / ultrastructure
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DNA Replication / genetics*
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism*
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Genes, Fungal*
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Mating Factor
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Mitosis / genetics
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Peptides / pharmacology
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Pheromones / pharmacology
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Plasmids
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Protein Tyrosine Phosphatases / genetics
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Protein Tyrosine Phosphatases / metabolism
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae Proteins*
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Transcription, Genetic*
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ras-GRF1*
Substances
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CDC6 protein, S cerevisiae
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Cell Cycle Proteins
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Fungal Proteins
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Oligodeoxyribonucleotides
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Peptides
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Pheromones
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Saccharomyces cerevisiae Proteins
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ras-GRF1
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Mating Factor
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Protein Tyrosine Phosphatases