Transgenic Zebrafish Expressing Rat Cytochrome P450 2E1 (CYP2E1): Augmentation of Acetaminophen-Induced Toxicity in the Liver and Retina
Abstract
:1. Introduction
2. Results
2.1. Generation of Transgenic Zebrafish Expressing Rat CYP2E1
2.2. Measurement of CYP2E1 Activity in Transgenic Zebrafish Expressing rat CYP2E1
2.3. Effects of Earlier Exposure to Acetaminophen on Zebrafish Embryos/Larvae
2.4. Effects of Later Exposure to Acetaminophen on Larval Zebrafish
2.5. Effects of APAP on Markers for Hepatocytes and Liver Primordium
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Transgenesis of Rat CYP2E1 into the Zebrafish Genome
4.3. Measurement of Rat CYP2E1 Activity
4.4. Zebrafish and Chemical Treatment
4.5. Measurement of the Size of the Retina and Liver
4.6. Measurement of Melanin
4.7. Whole-Mount In Situ Hybridization
4.8. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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p-nitropheonl | p-nitrocatechol | |
---|---|---|
Wild type | 1.577 ± 0.191 a | 0.052 ± 0.015 a |
EGFP [+] | 1.560 ± 0.123 a | 0.525 ± 0.113 b |
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Sato, Y.; Dong, W.; Nakamura, T.; Mizoguchi, N.; Nawaji, T.; Nishikawa, M.; Onaga, T.; Ikushiro, S.; Kobayashi, M.; Teraoka, H. Transgenic Zebrafish Expressing Rat Cytochrome P450 2E1 (CYP2E1): Augmentation of Acetaminophen-Induced Toxicity in the Liver and Retina. Int. J. Mol. Sci. 2023, 24, 4013. https://doi.org/10.3390/ijms24044013
Sato Y, Dong W, Nakamura T, Mizoguchi N, Nawaji T, Nishikawa M, Onaga T, Ikushiro S, Kobayashi M, Teraoka H. Transgenic Zebrafish Expressing Rat Cytochrome P450 2E1 (CYP2E1): Augmentation of Acetaminophen-Induced Toxicity in the Liver and Retina. International Journal of Molecular Sciences. 2023; 24(4):4013. https://doi.org/10.3390/ijms24044013
Chicago/Turabian StyleSato, Yoshinori, Wenjing Dong, Tatsuro Nakamura, Naohiro Mizoguchi, Tasuku Nawaji, Miyu Nishikawa, Takenori Onaga, Shinichi Ikushiro, Makoto Kobayashi, and Hiroki Teraoka. 2023. "Transgenic Zebrafish Expressing Rat Cytochrome P450 2E1 (CYP2E1): Augmentation of Acetaminophen-Induced Toxicity in the Liver and Retina" International Journal of Molecular Sciences 24, no. 4: 4013. https://doi.org/10.3390/ijms24044013