Fluorescence and Docking Studies of the Interaction between Human Serum Albumin and Pheophytin
Abstract
:1. Introduction
2. Results and Discussion
2.1. Molecular Absorption Spectroscopy in the UV-Vis
2.2. Fluorescence Spectroscopy
2.2.1. Binding Constant of Stern-Volmer Modified (Ka)
T (K) | Ka (L∙mol−1) | r2 |
---|---|---|
296 | 4.48 ± 0.06 × 104 | 0.9997 |
303 | 5.43 ± 0.13 × 104 | 0.9978 |
310 | 5.95 ± 0.08 × 104 | 0.9992 |
2.2.2. Thermodynamics Parameters (ΔG°, ΔH°, ΔS°)
T (K) | ΔH° (kJ∙mol−1) | ΔS° (kJ∙mol−1∙K−1) | ΔG° (kJ∙mol−1) | r2 |
---|---|---|---|---|
296 | −26.4 | |||
303 | 15.5 ± 1.2 | 0.145 | −27.4 | 0.9313 |
310 | −28.4 |
2.2.3. Fluorescence Quenching; Static vs. Dynamic
T (K) | KSV (L∙mol−1) | kq (L∙mol−1∙s−1) | r2 |
---|---|---|---|
296 | 1.75 ± 0.07 × 105 | 1.75 × 1013 | 0.9927 |
303 | 1.82 ± 0.03 × 105 | 1.82 × 1013 | 0.9980 |
310 | 2.03 ± 0.06 × 105 | 2.03 × 1013 | 0.9946 |
2.3. Molecular Modeling Calculation
3. Experimental Section
3.1. Spectroscopic Experiments
3.1.1. Instruments and Materials
3.1.2. Methodology for UV-Vis Spectroscopy
3.1.3. Methodology for Fluorescence Spectroscopy
3.2. Computational Experiments
Methodology for Docking Study
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Chaves, O.A.; Amorim, A.P.d.O.; Castro, L.H.E.; Sant’Anna, C.M.R.; De Oliveira, M.C.C.; Cesarin-Sobrinho, D.; Netto-Ferreira, J.C.; Ferreira, A.B.B. Fluorescence and Docking Studies of the Interaction between Human Serum Albumin and Pheophytin. Molecules 2015, 20, 19526-19539. https://doi.org/10.3390/molecules201019526
Chaves OA, Amorim APdO, Castro LHE, Sant’Anna CMR, De Oliveira MCC, Cesarin-Sobrinho D, Netto-Ferreira JC, Ferreira ABB. Fluorescence and Docking Studies of the Interaction between Human Serum Albumin and Pheophytin. Molecules. 2015; 20(10):19526-19539. https://doi.org/10.3390/molecules201019526
Chicago/Turabian StyleChaves, Otávio Augusto, Ana Paula de O. Amorim, Larissa H. E. Castro, Carlos Mauricio R. Sant’Anna, Márcia C. C. De Oliveira, Dari Cesarin-Sobrinho, José Carlos Netto-Ferreira, and Aurélio B. B. Ferreira. 2015. "Fluorescence and Docking Studies of the Interaction between Human Serum Albumin and Pheophytin" Molecules 20, no. 10: 19526-19539. https://doi.org/10.3390/molecules201019526