Aldevron’s Post

An engineered #T7 RNA Polymerase for efficient co-transcriptional #capping with reduced #dsRNA byproducts in #mRNA synthesis #IVT #mRNA #LNP https://lnkd.in/e4iMCBNt

#Article now available! #mRNA fragmentation and quality assessment using ion pair reverse-phase analytics This article demonstrates the application of an ion pair reverse phase chromatographic analytical method as a robust tool to determine #mRNAfragmentation while also separating in vitro transcription components from the main product. Read this article to: • Gain an overview of the challenges of mRNA fragmentation monitoring • Explore a versatile mRNA #qualitycontrol method • Learn about a method for determining the extent of mRNA fragmentation • Review a study assessing the stability of the mRNA analog at four different temperatures, determining the storage and shipping conditions Read below:

T7 RNA Polymerase ver.2.0 is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage T7 promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme uses double-stranded DNA containing T7 promoter sequence as a template and incorporates labeled or unlabeled nucleoside triphosphates to synthesize a single-stranded RNA transcript. Enhanced activity of T7 RNA Polymerase ver.2.0 enables the production of large quantities of high-quality RNA with high processivity and transcription frequency.

All 5 topics of the RNA Manufacturing Guided Tour Series are now all available, which addressed the audience question missed during our webinar, mRNA/saRNA Manufacturing: Considerations to Accelerate the Path to Clinic, presented by our Chief Scientific Officer, Dr. Venkata Indurthi.   Get the answers on how to move your RNA program from development to clinic. The complete series can be accessed through this link, so download each topic today.  https://hubs.li/Q01Xm-Y80

George Church and his team Harvard Medical School report the development and optimization of a platform for controlled, template-independent #enzymatic RNA #oligonucleotide synthesis. This enzymatic approach may address the length limitations commonly associated with phosphoramidite chemistry, as an enzymatic process has the potential for higher fidelity and fewer side reactions. Clearly exciting although more optimization is required to surpass chemical synthesis standards. https://lnkd.in/gsbDk-F6

Southern RNA's flagship Co Cap™ A , is a novel co-transcriptional capping reagent designed for mRNA synthesis. Co Cap™ A provides a robust 5' cap 1 structure, enhancing mRNA stability and translational efficiency. ✅ License-free and seamlessly integrated into the IVT process ✅ Requires a modified T7 RNA polymerase promoter. ✅ Full Quality Control testing complete with CoA ✅ Research or GMP-Grade available ✅ Sodium salt form and other Cap also available ✅ 24 month stability data ✅If you would like to receive a free trial sample, reach out to us to discuss - [email protected] A synthetic trinucleotide cap analog 𝗺𝟳𝗚𝗽𝗽𝗽(𝟮’-𝗢𝗠𝗲𝗔)𝗽𝗚, produces 𝟱’ 𝗖𝗮𝗽 𝟭 𝗺𝗥𝗡𝗔 when used as part of in vitro transcription (IVT) reaction. We supply in solution at 100±5 mM in various volumes (𝟮𝟬µ𝗟, 𝟭𝟬𝟬µ𝗟, 𝟭𝗺𝗹 & 𝟱𝗺𝗹). The larger the volume, the higher the discount available. Learn more about this cutting-edge solution at https://lnkd.in/g4gJTR-H #mRNA #Research #Innovation #Science #SouthernRNA #NothernRNA #CoCapA #IVT

#mRNAtechnology skyrocketed to fame during the COVID-19 pandemic; now it’s being considered for a variety of groundbreaking applications. Despite its progress, mRNA process development still has several areas of improvement, including the purification process required to remove contaminants, such as dsRNA and residual host cell DNA. Our new blog explores the advantages and disadvantages of different purification methods and explains how the right #CDMO partner can help develop a strong #mRNApurification strategy for your project’s unique needs. Read the blog: http://spr.ly/6044PokpA

📣 Please check the post below if you are interested in structural characterization of RNA!

Lipid nanoparticles (LNPs) are commonly used to deliver mRNA, and cationic lipids can significantly affect biodistribution, cellular uptake, endosomal escape, and transfection efficiency. However, cationic lipids are difficult to synthesize in the laboratory, which limits the discovery of suitable lipids and hinders large-scale production. In view of this, the researchers reported a one-pot series of multi-component reaction and designed a multi-component conjugation of amine-thiol-acrylate, which enabled the rapid synthesis (1 h) of degradable lipids containing amidines at room temperature. https://lnkd.in/g9eQxA8h