This was achieved by determining whether there was a significant increase in absolute
reticulocyte count after initiation of iron therapy and whether the increase in absolute
reticulocyte count was early and cost effective.
The inference that it was the internal contents of the
reticulocyte staining and not anything more sinister was supported by both the high
reticulocyte count and the fact that the inclusions were only found in the polychromatic cells.
In laboratory evidences, normochromic and normocytic anemia, normalized number of
reticulocytes formed by reticulocytosis, which the response of the bone marrow to the increased peripheral degradation, is >5% (2).
Lower vitamin D levels were associated with lower hemoglobin and hematocrit but with higher
reticulocyte counts, AST, LDH, and total and indirect serum bilirubin levels.
A summary of the E%(FECH), red cell, and
reticulocyte parameters of the subjects is shown in Table 3, Table 4, and Fig.
Despite a low
reticulocyte count, she was able to achieve sustained virologic response (SVR) with the combination of sofosbuvir (SOF) and RBV.
His initial hematocrit was 44% with a 1.9%
reticulocyte count.
(5,7) Other testing beneficial to diagnosing the cause of the anemia would be
reticulocyte indices, iron studies, and fecal blood testing.
Laboratory analysis revealed hemoglobin 5.6 g/dL, hematocrit 16.9%, platelet count 92 x 109/L, white blood cell count 6.4 x 109/L,
reticulocyte count 3.3%, mean corpuscular volume 113.2 fL, lactate dehydrogenase 3137 IU/L, homocysteine 69.5 [micro]mol/L, and normal creatinine.
The
reticulocyte count was determined from blood smears after supravital staining with methylene blue and reported as a percentage of circulating RBCs.
These include biochemical parameters, namely zinc protoporphyrin and soluble transferrin receptor, as well as haematological parameters, namely the percentage of hypochromic red blood cells, and
reticulocyte parameters such as the
reticulocyte haemoglobin content (CHr).
While infection of WT mice caused a significant decrease in
reticulocyte numbers, infection of IFNa[sup]−/− or IFNa-R[sup]−/− mice did not alter
reticulocyte numbers as compared with sham controls [Figure 3]b.
Reticulocyte count was significantly greater in LHTH compared with CONTROL at 2 (p = 0.02) but not at 3 weeks (p = 0.27) of exposure.
The method is an integral part of the
reticulocyte assay and is based on a proprietary fluorescent dye (10) and an optimized flow cytometric method.
