A vesicle is a small bubble enclosed by lipid bilayer. Vesicles can form naturally, for example, during the endocytosis, or they may be prepared. Artificially prepared vesicles are known as liposomes. If there is only one phospholipid bilayer, they are called unilamellar vesicles; otherwise they are called multilamellar.
Vesicles do many things. The membrane enclosing the vesicle is similar to that of the plasma membrane, and vesicles can fuse with the plasma membrane to release their contents outside of the cell. Vesicles can also fuse with other organelles within the cell.
Because it is separated from the cytosol, the inside of the vesicle can be made to be different from the cytosolic environment. For this reason, vesicles are a basic tool used by the cell for organizing cellular substances. Vesicles are involved in metabolism, transport, buoyancy control,[1] and enzyme storage. They can also act as chemical reaction chambers.
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Types of vesicles [link]
Vacuoles [link]
Vacuoles are vesicles which contain mostly water.
- Plant cells are known for having a large central vacuole in the center of the cell that is used for osmotic control and nutrient storage.
- Contractile vacuoles are found in certain protists, especially those in Phylum Ciliophora. These vacuoles take water from the cytoplasm and excrete it from the cell to avoid bursting due to osmotic pressure.
Lysosomes [link]
- Lysosomes or "Suicide Cells" are involved in cellular digestion. Food can be taken from outside the cell into food vacuoles by a process called endocytosis. These food vacuoles fuse with lysosomes which break down the components so that they can be used in the cell. This form of cellular eating is called phagocytosis.
- Lysosomes are also used to destroy defective or damaged organelles in a process called endophagocytosis. They fuse with the membrane of the damaged organelle digesting it.
Transport vesicles [link]
- Transport vesicles can move molecules between locations inside the cell, e.g., proteins from the rough endoplasmic reticulum to the Golgi apparatus.
- Membrane-bound and secreted proteins are made on ribosomes found in the rough endoplasmic reticulum. Most of these proteins mature in the Golgi apparatus before going to their final destination which may be to lysosomes, peroxisomes, or outside of the cell. These proteins travel within the cell inside of transport vesicles.
Secretory vesicles [link]
Secretory vesicles contain materials that are to be excreted from the cell. Cells have many reasons to excrete materials. One reason is to dispose of wastes. Another reason is tied to the function of the cell. Within a larger organism, some cells are specialized to produce certain chemicals. These chemicals are stored in secretory vesicles and released when needed. Some examples include the following.
Types of secretory vesicles [link]
- Synaptic vesicles are located at presynaptic terminals in neurons and store neurotransmitters. When a signal comes down an axon, the synaptic vesicles fuse with the cell membrane releasing the neurotransmitter so that it can be detected by receptor molecules on the next nerve cell.
- In animals endocrine tissues release hormones into the bloodstream. These hormones are stored within secretory vesicles. A good example is the endocrine tissue found in the islets of Langerhans in the pancreas. This tissue contains many cell types that are defined by which hormones they produce.
- Secretory vesicles hold the enzymes that are used to make the cell walls of plants, protists, fungi, bacteria, and Archaea cells as well as the extracellular matrix of animal cells.
Other types of vesicles [link]
- Gas vesicles are used by Archaea, bacteria and planktonic microorganisms, possibly to control vertical migration by regulating the gas content and thereby buoyancy, or possibly to position the cell for maximum solar light harvesting.
- Matrix vesicles are located within the extracellular space, or matrix. Using electron microscopy but working independently, they were discovered in 1967 by H. Clarke Anderson[2] and Ermanno Bonucci.[3] These cell-derived vesicles are specialized to initiate biomineralisation of the matrix in a variety of tissues, including bone, cartilage, and dentin. During normal calcification, a major influx of calcium and phosphate ions into the cells accompanies cellular apoptosis (genetically determined self-destruction) and matrix vesicle formation. Calcium-loading also leads to formation of phosphatidylserine:calcium:phosphate complexes in the plasma membrane mediated in part by a protein called annexins. Matrix vesicles bud from the plasma membrane at sites of interaction with the extracellular matrix. Thus, matrix vesicles convey to the extracellular matrix calcium, phosphate, lipids and the annexins which act to nucleate mineral formation. These processes are precisely coordinated to bring about, at the proper place and time, mineralization of the tissue's matrix unless the Golgi are non-existent.
- Multivesicular body, or MVB, is a membrane-bound vesicle containing a number of smaller vesicles.
Vesicle formation and transport [link]
(1) Nucleolus
(2) Nucleus
(3) Ribosomes (little dots)
(4) Vesicle
(5) Rough endoplasmic reticulum (ER)
(6) Golgi apparatus
(7) Cytoskeleton
(8) Smooth ER
(9) Mitochondria
(10) Vacuole
(11) Cytosol
(12) Lysosome
(13) Centrioles within Centrosome
Some vesicles are made when part of the membrane pinches off the endoplasmic reticulum or the Golgi complex. Others are made when an object outside of the cell is surrounded by the cell membrane.
Capturing cargo molecules [link]
The assembly of vesicles requires numerous coats to surround and bind to the proteins being transported. these bind to the coat vesicle. They also trap various transmembrane receptor proteins, called cargo receptors, which in turn trap the cargo molecules.
Vesicle coat [link]
The vesicle coat serves to sculpt the curvature of a donor membrane, and to select specific proteins as cargo. It selects cargo proteins by binding to sorting signals. In this way the vesicle coat clusters selected membrane cargo proteins into nascent vesicle buds.
There are three types of vesicle coats: clathrin, COPI and COPII. Clathrin coats are found on vesicles trafficking between the Golgi and plasma membrane, the Golgi and endosomes, and the plasma membrane and endosomes. COPI coated vesicles are responsible for retrograde transport from the Golgi to the ER, while COPII coated vesicles are responsible for anterograde transport from the ER to the Golgi.
The clathrin coat is thought to assemble in response to regulatory G protein. A coatomer coat assembles and disassembles due to an ARF protein.
Vesicle docking [link]
Surface markers called SNAREs identify the vesicle's cargo, and complementary SNAREs on the target membrane act to cause fusion of the vesicle and target membrane. Such v-SNARES are hypothesised to exist on the vesicle membrane, while the complementary ones on the target membrane are known as t-SNAREs.
Often SNAREs associated with vesicles or target membranes are instead classified as Qa, Qb, Qc or R SNAREs owing to further variation than simply v- or t-SNAREs. An array of different SNARE complexes can be seen in different tissues and subcellular compartments, with 36 isoforms currently identified in humans.
Regulatory Rab proteins are thought to inspect the joining of the SNAREs. Rab protein is a regulatory GTP-binding protein, and controls the binding of these complementary SNAREs for a long enough time for the Rab protein to hydrolyse its bound GTP and lock the vesicle onto the membrane.
Vesicle fusion [link]
Vesicle fusion can occur in one of two ways: full fusion or kiss-and-run fusion. Fusion requires the two membranes to be brought within 1.5 nm of each other. For this to occur water must be displaced from the surface of the vesicle membrane. This is energetically unfavourable, and evidence suggests that the process requires ATP, GTP and acetyl-coA, fusion is also linked to budding, which is why the term budding and fusing arises.
Vesicles in receptor downregulation [link]
Membrane proteins serving as receptors are sometimes tagged for downregulation by the attachment of ubiquitin. After arriving an endosome via the pathway described above, vesicles begin to form inside the endosome, taking with them the membrane proteins meant for degradation; When the endosome either matures to become a lysosome or is united with one, the vesicles are completely degraded. Without this mechanism, only the extracellular part of the membrane proteins would reach the lumen of the lysosome, and only this part would be degraded.[4]
It is because of these vesicles that the endosome is sometimes known as a multivesicular body. The pathway to their formation is not completely understood; unlike the other vesicles described above, the outer surface of the vesicles is not in contact with the cytosol.
Vesicle preparation [link]
Isolated vesicles [link]
Producing membrane vesicles is one of the methods to investigate various membranes of the cell. After the living tissue is crushed into suspension, various membranes form tiny closed bubbles. Big fragments of the crushed cells can be later discarded by low speed centrifugation and later the fraction of the known origin (plasmalemma, tonoplast, etc.) can be isolated by precise high speed centrifugation in the density gradient. Using osmotic shock, it is possible temporarily open vesicles (filling in them with the required solution) and then centrifugate down again and resuspend in a different solution. Applying ionophores like valinomycin can create electrochemic gradients that are comparable to the gradients inside the living cell.
Vesicles are mainly used in two types of research:
- To find and later isolate membrane receptors that specifically bind hormones and various other important substances.[5]
- To investigate transport of various ions or other substances across the membrane of the given type.[6] While the transport can be easier investigated with patch clamp techniques, vesicles can also be isolated from objects for that the patch clamp is not applicable.
Artificial vesicles [link]
Phospholipid vesicles have also been studied in biochemistry. For such studies, a homogeneous phospholipid vesicle suspension can be prepared by sonication,[7] injection of a phospholipid solution into the aqueous buffer solution membranes.[8] In this way aqueous vesicle solutions can be prepared of different phospholipid composition, as well as different sizes of vesicles.
See also [link]
- Endoplasmic reticulum
- Golgi apparatus
- Micelle
- Membrane nanotube
- Endocytosis
- Synaptic vesicle
- Membrane contact sites
- Spitzenkörper
- DODAB
References [link]
- ^ Walsby AE (1994). "Gas vesicles". Microbiological reviews 58 (1): 94–144. PMC 372955. PMID 8177173. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=372955.
- ^ Anderson HC (1967). "Electron microscopic studies of induced cartilage development and calcification". J. Cell Biol. 35 (1): 81–101. DOI:10.1083/jcb.35.1.81. PMC 2107116. PMID 6061727. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2107116.
- ^ Bonucci E (1967). "Fine structure of early cartilage calcification". J. Ultrastruct. Res. 20 (1): 33–50. DOI:10.1016/S0022-5320(67)80034-0. PMID 4195919.
- ^ Katzmann DJ, Odorizzi G, Emr SD (2002). "Receptor downregulation and multivesicular-body sorting" (pdf). Nat. Rev. Mol. Cell Biol. 3 (12): 893–905. DOI:10.1038/nrm973. PMID 12461556. https://www.colorado.edu/MCDB/odorizzilab/katzmann2002.pdf.
- ^ Sidhu, V.K., F.-J. Vorhölter, K. Niehaus, & S.A. Watt. (2008). Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris. BMC Microbiology, 8:87
- ^ Günther G.F.E. Scherer, Georg Martiny-Baron (1985) K+/H+ exchange transport in plantmembranevesicles is evidence for K+ transport. Plant Science, 41 (3), 161–168
- ^ Barenholz, Y.; Gibbes, D.; Litman, B. J.; Goll, J.; Thompson, T. E.; Carlson, F. D. (1977). "A simple method for the preparation of homogeneous phospholipid vesicles". Biochemistry 16 (12): 2806. DOI:10.1021/bi00631a035. PMID 889789.
- ^ Batzri, S; Korn, E (1973). "Single bilayer liposomes prepared without sonication". Biochimica et Biophysica Acta (BBA) - Biomembranes 298: 1015. DOI:10.1016/0005-2736(73)90408-2.
Further reading [link]
- Bruce Alberts, et al. (1994); Molecular Biology of the Cell; Third Edition
External links [link]
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Biology
Biology is a natural science concerned with the study of life and living organisms, including their structure, function, growth, evolution, distribution, and taxonomy. Modern biology is a vast and eclectic field, composed of many branches and subdisciplines. However, despite the broad scope of biology, there are certain general and unifying concepts within it that govern all study and research, consolidating it into single, coherent fields. In general, biology recognizes the cell as the basic unit of life, genes as the basic unit of heredity, and evolution as the engine that propels the synthesis and creation of new species. It is also understood today that all organisms survive by consuming and transforming energy and by regulating their internal environment to maintain a stable and vital condition.
Subdisciplines of biology are defined by the scale at which organisms are studied, the kinds of organisms studied, and the methods used to study them: biochemistry examines the rudimentary chemistry of life; molecular biology studies the complex interactions among biological molecules; botany studies the biology of plants; cellular biology examines the basic building-block of all life, the cell; physiology examines the physical and chemical functions of tissues, organs, and organ systems of an organism; evolutionary biology examines the processes that produced the diversity of life; and ecology examines how organisms interact in their environment.
Biology (journal)
Biology is a quarterly, peer-reviewed, open access, scientific journal covering research on all aspects of biology. It was established in 2012 and is published by MDPI. The editor-in-chief is Chris O'Callaghan (University of Oxford). The journal publishes reviews, research papers, and communications.
Subject Areas
This journal covers all topics related to biology. More detail about the journal and its scope is available in the first editorial. Research fields of interest include, but are not limited to:
Biology: The Unity and Diversity of Life
Biology: The Unity and Diversity of Life is a college-level introductory biology textbook that covers basic biochemistry, anatomy, taxonomy, evolution, biotechnology and ecology. The book is designed to create in students a broad foundation of knowledge in biology and is frequently used in AP Biology classes in American high schools. Each book includes an interactive CD-ROM with links to additional instructional material. Like many textbooks new versions are printed every few years and reflect new gains in scientific knowledge. The book is published by Brooks/Cole and is currently in its twelfth edition. It was primarily compiled by Cecie Starr and Ralph Taggart with pictures and illustrations by Lisa Starr.
The book was translated into few languages, among others into Hebrew, published by the Open University of Israel.
External links
Vesicle
Vesicle may refer to:
See also
Cutaneous condition
A cutaneous condition is any medical condition that affects the integumentary system—the organ system that encloses the body and includes skin, hair, nails, and related muscle and glands. The major function of this system is as a barrier against the external environment.
Conditions of the human integumentary system constitute a broad spectrum of diseases, also known as dermatoses, as well as many nonpathologic states (like, in certain circumstances, melanonychia and racquet nails). While only a small number of skin diseases account for most visits to the physician, thousands of skin conditions have been described. Classification of these conditions often presents many nosological challenges, since underlying etiologies and pathogenetics are often not known. Therefore, most current textbooks present a classification based on location (for example, conditions of the mucous membrane), morphology (chronic blistering conditions), etiology (skin conditions resulting from physical factors), and so on.
Vesicular texture
Vesicular texture is a volcanic rock texture characterized by a rock being pitted with many cavities (known as vesicles) at its surface and inside. This texture is common in aphanitic, or glassy, igneous rocks that have come to the surface of the earth, a process known as Extrusion. As magma rises to the surface the pressure on it decreases. When this happens gasses dissolved in the magma are able to come out of solution, forming gas bubbles (the cavities) inside it. When the magma finally reaches the surface as lava and cools, the rock solidifies around the gas bubbles and traps them inside, preserving them as holes filled with gas called vesicles.
A related texture is amygdaloidal in which the volcanic rock, usually basalt or andesite, has cavities, or vesicles, that are filled with secondary minerals, such as zeolites, calcite, quartz, or chalcedony. Individual cavity fillings are termed amygdules (American usage), or amygdales (British usage). Sometimes these can be sources of semi-precious stones such as agate.
