Ube2m

Ubiquitin-conjugating enzyme E2M

PDB rendering based on 1y8x.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1TT5, 1Y8X, 2NVU, 3TDU, 3TDZ

Identifiers

Symbols

UBE2M; UBC-RS2; UBC12; hUbc12

External IDs

OMIM: 603173 MGI: 108278 HomoloGene: 2952 GeneCards: UBE2M Gene

EC number

6.3.2.19

Gene Ontology
Molecular function	• nucleotide binding • ubiquitin-protein ligase activity • ubiquitin-protein ligase activity • protein binding • ATP binding • ligase activity • acid-amino acid ligase activity • ribosomal S6-glutamic acid ligase activity • NEDD8 ligase activity
Cellular component	• cellular_component
Biological process	• protein modification process • protein modification process • protein ubiquitination • protein ubiquitination • positive regulation of neuron apoptosis • protein neddylation
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 19:
59.07 – 59.07 Mb

Chr 7:
13.62 – 13.62 Mb

PubMed search

[1]

[2]

NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene.^[1]^[2]

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.^[2]

Interactions [link]

UBE2M has been shown to interact with NEDD8,^[3] PRKAR1A^[4] and UBA3.^[5]

References [link]

^ Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S (Aug 1998). "A new NEDD8-ligating system for cullin-4A". Genes Dev 12 (15): 2263–8. DOI:10.1101/gad.12.15.2263. PMC 317039. PMID 9694792. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=317039.
^ ^a ^b "Entrez Gene: UBE2M ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9040.
^ Gong, L; Yeh E T (Apr. 1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway". J. Biol. Chem. (UNITED STATES) 274 (17): 12036–42. DOI:10.1074/jbc.274.17.12036. ISSN 0021-9258. PMID 10207026.
^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. DOI:10.1038/msb4100134. PMC 1847948. PMID 17353931. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.
^ Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA (February 2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1". Mol. Cell 17 (3): 341–50. DOI:10.1016/j.molcel.2004.12.020. PMID 15694336.

Further reading [link]

Gong L, Yeh ET (1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway". J. Biol. Chem. 274 (17): 12036–42. DOI:10.1074/jbc.274.17.12036. PMID 10207026.
Chen Y, McPhie DL, Hirschberg J, Neve RL (2000). "The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons". J. Biol. Chem. 275 (12): 8929–35. DOI:10.1074/jbc.275.12.8929. PMID 10722740.
Wada H, Yeh ET, Kamitani T (2000). "A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo". J. Biol. Chem. 275 (22): 17008–15. DOI:10.1074/jbc.275.22.17008. PMID 10828074.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. DOI:10.1073/pnas.242603899. PMC 139241. PMID 12477932. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Tomsig JL, Snyder SL, Creutz CE (2003). "Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif". J. Biol. Chem. 278 (12): 10048–54. DOI:10.1074/jbc.M212632200. PMID 12522145.
Huang DT, Miller DW, Mathew R et al. (2004). "A unique E1–E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8". Nat. Struct. Mol. Biol. 11 (10): 927–35. DOI:10.1038/nsmb826. PMC 2862556. PMID 15361859. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2862556.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. DOI:10.1101/gr.2596504. PMC 528928. PMID 15489334. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Huang DT, Paydar A, Zhuang M et al. (2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1". Mol. Cell 17 (3): 341–50. DOI:10.1016/j.molcel.2004.12.020. PMID 15694336.
Li T, Santockyte R, Shen RF et al. (2006). "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers". Arch. Biochem. Biophys. 453 (1): 70–4. DOI:10.1016/j.abb.2006.03.002. PMID 16620772.
Ewing RM, Chu P, Elisma F et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. DOI:10.1038/msb4100134. PMC 1847948. PMID 17353931. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.

PDB gallery

1y8x: Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1

2nvu: Structure of APPBP1-UBA3~NEDD8-NEDD8-MgATP-Ubc12(C111A), a trapped ubiquitin-like protein activation complex

Posttranslational modification

Chaperones/
protein folding

Heat shock proteins/ Chaperonins	Hsp10/GroES (Early pregnancy factor) · Hsp27 · Hsp47 · HSP60/GroEL Hsp40/DnaJ (A1, A2, A3, B1, B2, B11, B4, B6, B9, C1, C3, C5, C6, C7, C10, C11, C13, C14, C19) Hsp70 (1A, 1B, 1L, 2, 4, 4L, 5, 6, 7, 8, 9, 12A, 14) Hsp90 (α₁, α₂, β, ER, TRAP1)

Other	Alpha crystallin · Clusterin · Survival of motor neuron (SMN1, SMN2)

Protein targeting

Signal peptide

Ubiquitin

E1 Ubiquitin-activating enzyme (UBA1, UBA2, UBA3, UBA5, UBA6, UBA7, ATG7, NAE1, SAE1)

E2 Ubiquitin-conjugating enzyme (A • B • C • D1, D2, D3 • E1, E2, E3 • G1, G2 • H • I • J1, J2 • K • L1, L2, L3, L4, L6 • M • N • O • Q1, Q2 • R1 (CDC34), R2 • S • V1, V2 • Z)

E3 Ubiquitin ligase (VHL, Cullin, CBL, MDM2, FANCL, UBR1)

Deubiquitinating enzyme: Ataxin 3 • USP6 • CYLD

ATG3 • BIRC6 • UFC1

Other

SUMO protein

see also posttranslational modification disorders
B bsyn: dna (repl, cycl, reco, repr) · tscr (fact, tcrg, nucl, rnat, rept, ptts) · tltn (risu, pttl, nexn) · dnab, rnab/runp · stru (domn, 1°, 2°, 3°, 4°)

This article on a gene on chromosome 19 is a stub. You can help Wikipedia by expanding it.

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Interactions [link]

References [link]

Further reading [link]

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