Ago

Ago or AGO may refer to:

People

Places

Other uses

Argonaute

The Argonaute protein family plays a central role in RNA silencing processes, as essential catalytic components of the RNA-induced silencing complex (RISC). RISC complex is responsible for the gene silencing phenomenon known as RNA interference (RNAi). Argonaute proteins bind different classes of small non-coding RNAs, including microRNAs (miRNAs), small interfering RNAs (siRNAs) and Piwi-interacting RNAs (piRNAs). Small RNAs guide Argonaute proteins to their specific targets through sequence complementarity (base pairing), which then leads to mRNA cleavage or translation inhibition.

Discovery

The RNA interference (RNAi) was first reported in 1995 by Guo and Kemphues, and similar pathways collectively referred to as RNA silencing were discovered in plants and fungi. The beginning of people’s understanding of the mechanism of RNA silencing began only in 1998 with the experiments of Fire and colleagues demonstrating that double-stranded RNA triggered RNAi. RNA silencing pathways process long RNAs into small RNAs that direct the repression of transcription or translation of nucleic acid targets with sequence corresponding to the small RNAs. These single-stranded RNAs, referred to as guide strands, are incorporated into RNA silencing effectors complexes such as the RNA-induced silencing complex (RISC). These RNA silencing effector complexes contain Argonaute family proteins.

Traffic

Traffic on roads may consist of pedestrians, ridden or herded animals, vehicles, streetcars, buses and other conveyances, either singly or together, while using the public way for purposes of travel. Traffic laws are the laws which govern traffic and regulate vehicles, while rules of the road are both the laws and the informal rules that may have developed over time to facilitate the orderly and timely flow of traffic.

Organized traffic generally has well-established priorities, lanes, right-of-way, and traffic control at intersections.

Traffic is formally organized in many jurisdictions, with marked lanes, junctions, intersections, interchanges, traffic signals, or signs. Traffic is often classified by type: heavy motor vehicle (e.g., car, truck); other vehicle (e.g., moped, bicycle); and pedestrian. Different classes may share speed limits and easement, or may be segregated. Some jurisdictions may have very detailed and complex rules of the road while others rely more on drivers' common sense and willingness to cooperate.

Traffic (2000 film)

Traffic is a 2000 American crime drama film directed by Steven Soderbergh and written by Stephen Gaghan. It explores the illegal drug trade from a number of perspectives: a user, an enforcer, a politician and a trafficker. Their stories are edited together throughout the film, although some of the characters do not meet each other. The film is an adaptation of the British Channel 4 television series Traffik.

20th Century Fox, the original financiers of the film, demanded Harrison Ford play a leading role and that significant changes to the screenplay be made. Soderbergh refused and proposed the script to other major Hollywood studios, but it was rejected because of the three-hour running time and the subject matter — Traffic is more of a political film than most Hollywood productions. USA Films, however, liked the project from the start and offered the film-makers more money than Fox. Soderbergh operated the camera himself and adopted a distinctive cinematography tint for each story so that audiences could tell them apart.

Traffic (ABC album)

Traffic is the first new album of original material released by English band ABC in eleven years. The album's songs were written whilst the band toured the United States in 2006. Critics have described the album as the most 'satisfying ABC album since the mid-'80s by far'.

Drummer David Palmer, who left the band in 1982 after recording The Lexicon of Love, returned to record this album, for which he co-wrote all of the tracks.

Gary Langan returned to mix the album after working as sound engineer on The Lexicon of Love and producing Beauty Stab.

Allmusic described Traffic as 'the album that ABC fans were probably hoping for in 1985' and considered that Fry's 'lyrical mastery was back in place' in the album's songs which showed 'an elegant mix of soul and style'.

Track listing

All songs written and composed by Martin Fry, Chuck Kentis, and David Palmer.