Transfer RNA

A transfer RNA (abbreviated tRNA and archaically referred to as sRNA, for soluble RNA) is an adaptor molecule composed of RNA, typically 76 to 90 nucleotides in length, that serves as the physical link between the mRNA and the amino acid sequence of proteins. It does this by carrying an amino acid to the protein synthetic machinery of a cell (ribosome) as directed by a three-nucleotide sequence (codon) in a messenger RNA (mRNA). As such, tRNAs are a necessary component of translation, the biological synthesis of new proteins according to the genetic code.

The specific nucleotide sequence of an mRNA specifies which amino acids are incorporated into the protein product of the gene from which the mRNA is transcribed, and the role of tRNA is to specify which sequence from the genetic code corresponds to which amino acid. One end of the tRNA matches the genetic code in a three-nucleotide sequence called the anticodon. The anticodon forms three base pairs with a codon in mRNA during protein biosynthesis. The mRNA encodes a protein as a series of contiguous codons, each of which is recognized by a particular tRNA. On the other end of the tRNA is a covalent attachment to the amino acid that corresponds to the anticodon sequence. Each type of tRNA molecule can be attached to only one type of amino acid, so each organism has many types of tRNA (in fact, because the genetic code contains multiple codons that specify the same amino acid, there are several tRNA molecules bearing different anticodons which also carry the same amino acid).

TRNA (guanine26-N2)-dimethyltransferase

TRNA (guanine²⁶-N²)-dimethyltransferase (EC 2.1.1.216, Trm1p, TRM1, tRNA (m22G26)dimethyltransferase) is an enzyme with system name S-adenosyl-L-methionine:tRNA (guanine²⁶-N²)-dimethyltransferase. This enzyme catalyses the following chemical reaction

The enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions.

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TRNA (cytidine34-2'-O)-methyltransferase

tRNA (cytidine³⁴-2'-O)-methyltransferase (EC 2.1.1.207, yibK (gene), methyltransferase yibK, TrmL, tRNA methyltransferase L, tRNA (cytidine^34/5-carboxymethylaminomethyluridine³⁴-2'-O)-methyltransferase) is an enzyme with system name S-adenosyl-L-methionine:tRNA (cytidine^34/5-carboxymethylaminomethyluridine³⁴-2'-O)-methyltransferase. This enzyme catalyses the following chemical reaction

The enzyme from Escherichia coli catalyses the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNALeuCmAA and tRNALeucmnm5UmAA.

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