Staining

Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.

In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.

Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining.

Biology

Biology is a natural science concerned with the study of life and living organisms, including their structure, function, growth, evolution, distribution, and taxonomy. Modern biology is a vast and eclectic field, composed of many branches and subdisciplines. However, despite the broad scope of biology, there are certain general and unifying concepts within it that govern all study and research, consolidating it into single, coherent fields. In general, biology recognizes the cell as the basic unit of life, genes as the basic unit of heredity, and evolution as the engine that propels the synthesis and creation of new species. It is also understood today that all organisms survive by consuming and transforming energy and by regulating their internal environment to maintain a stable and vital condition.

Subdisciplines of biology are defined by the scale at which organisms are studied, the kinds of organisms studied, and the methods used to study them: biochemistry examines the rudimentary chemistry of life; molecular biology studies the complex interactions among biological molecules; botany studies the biology of plants; cellular biology examines the basic building-block of all life, the cell; physiology examines the physical and chemical functions of tissues, organs, and organ systems of an organism; evolutionary biology examines the processes that produced the diversity of life; and ecology examines how organisms interact in their environment.

Biology (journal)

Biology is a quarterly, peer-reviewed, open access, scientific journal covering research on all aspects of biology. It was established in 2012 and is published by MDPI. The editor-in-chief is Chris O'Callaghan (University of Oxford). The journal publishes reviews, research papers, and communications.

Subject Areas

This journal covers all topics related to biology. More detail about the journal and its scope is available in the first editorial. Research fields of interest include, but are not limited to:

Biology: The Unity and Diversity of Life

Biology: The Unity and Diversity of Life is a college-level introductory biology textbook that covers basic biochemistry, anatomy, taxonomy, evolution, biotechnology and ecology. The book is designed to create in students a broad foundation of knowledge in biology and is frequently used in AP Biology classes in American high schools. Each book includes an interactive CD-ROM with links to additional instructional material. Like many textbooks new versions are printed every few years and reflect new gains in scientific knowledge. The book is published by Brooks/Cole and is currently in its twelfth edition. It was primarily compiled by Cecie Starr and Ralph Taggart with pictures and illustrations by Lisa Starr.

The book was translated into few languages, among others into Hebrew, published by the Open University of Israel.

External links

Stain

A stain is a discoloration that can be clearly distinguished from the surface, material, or medium it is found upon. They are caused by the chemical or physical interaction of two dissimilar materials. Staining is used for biochemical research, metal staining, and art (e.g., wood staining, stained glass).

Types of stains

There can be intentional stains (such as wood stains or paint), indicative stains (such as food coloring or adding a substance to make bacteria visible under a microscope), natural stains (such as rust on iron or a patina on bronze), and accidental stains such as ketchup on a shirt.

Different types of material can be stained by different substances, and stain resistance is an important characteristic in modern textile engineering.

Formation

The primary method of stain formation is surface stains, where the staining substance is spilled out onto the surface or material and is trapped in the fibers, pores, indentations, or other capillary structures on the surface. The material that is trapped coats the underlying material, and the stain reflects back light according to its own color. Applying paint, spilled food, and wood stains are of this nature.

Stain (album)

Stain is the third album by Living Colour. It was released on March 2, 1993, by Epic Records. It is the first album to feature bassist Doug Wimbish. Stain features a much heavier and aggressive Living Colour, containing elements of thrash metal and industrial music. It reached No. 26 on the Billboard 200. The band split up in January 1995 following a tour with Bad Brains.

The cover art shows a woman wearing a brank.

From the mid-1990s through the early 2010s, the album was out of print due to rights issues after a lawsuit from Jon Stainbrook of the band The Stain forced Sony to cease production of the album. In November 2013, the album was reissued by Music On CD and is also available as an MP3 download.

Track listing

Chart performance

Singles

Personnel

Stain (EP)

Stain is the first EP release by the Canadian rock band Mystery Machine.

Track listing