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Radioimmunoprecipitation assay buffer is a lysis buffer used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA).^[1][2] This buffer is more denaturing than NP-40 or Triton X-100 lysis buffer because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for nuclear membrane disruption for nuclear extracts. The RIPA buffer gives low background but can denature kinases.

Recipe [link]

RIPA buffer recipe for total protein extract is as follows:

• Tris 50mM
• NaCl 150 mM
• SDS 0.1 %
• Na.Deoxycholate 0.5 %
• Triton X 100 or NP-40 1%
• Protease inhibitors such as PMSF or cocktail protease inhibitors which are commercially available (use according to the manufacturer's guide)

References [link]

External links [link]

• 1X RIPA Buffer

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