Ribonuclease H
Ribonuclease H (RNase H) is a family of non-sequence-specific endonucleases that catalyze the cleavage of RNA via a hydrolytic mechanism. Members of the RNase H family can be found in nearly all organisms, from bacteria to archaea to eukaryotes.
RNase H’s ribonuclease activity cleaves the 3’-O-P bond of RNA in a DNA/RNA duplex substrate to produce 3’-hydroxyl and 5‘-phosphate terminated products. In DNA replication, RNase H is responsible for removing the RNA primer, allowing completion of the newly synthesized DNA.
Structure
The 3-D structure of RNase H commonly consists of a 5-stranded β-sheet surrounded by a distribution of α-helices. In some RNase H, such as the one found in HIV-1, the enzyme is missing one of the helices known as the C-helix, a positively charged α-helix whose protrusive shape increases substrate binding capacity. The active site of the enzyme is centered around a conserved DEDD motif (composed of residues: D443, E478, D498, and D549) which performs the hydrolysis of the RNA substrate. A magnesium ion is commonly used as a cofactor during the hydrolysis step. It is also a potential but unconfirmed mechanism in which multiple ions are necessary to perform the hydrolysis. The enzyme also contains a nucleic acid binding cleft about 60 Å in length that can encompass a region of 18 bound RNA/DNA base pairs.
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Don't Go Away
by: RunawaysI met you last night, it was really alright
We got along
We partied awhile, you gave me the smile
Nothin' went wrong
Hold me close
Hold me really tight
Go ahead
Make me feel alright
Don't go away, I'm so happy
You're just what I need to make it
Don't go away, I'm so happy
You're just what I need
The very next day, you saw us play
It was really a kick
Drippin' in sweat, I got very wet, honey
You did the trick
Hold me close
Hold me really tight
Go ahead
Make me feel alright
Don't go away, I'm so happy
You're just what I need to make it
Don't go away, I'm so happy
You're just what I need
Hold me close
Hold me really tight
Go ahead
Make me feel alright
Don't go away, I'm so happy
You're just what I need to make it
Don't go away, I'm so happy
You're just what I need
I stayed the night, it was really alright
I need our heat
I got kinda cold, you got really bold
Ya said, come to me
Hold me close
Hold me really tight
Go ahead
Make me feel alright
Don't go away, I'm so happy
You're just what I need to make it
Don't go away, I'm so happy
You're just what I need
Don't go away, I'm so happy
You're just what I need to make it
Don't go away, I'm so happy
You're just what I need
Don't go away, I'm so happy
You're just what I need to make it
Don't go away, I'm so happy
You're just what I need
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