Rbx1

Ring-box 1, E3 ubiquitin protein ligase

PDB rendering based on 1ldj.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1LDJ, 1LDK, 1U6G, 2HYE, 2LGV, 3DPL, 3DQV, 3RTR

Identifiers

Symbols

RBX1; BA554C12.1; RNF75; ROC1

External IDs

OMIM: 603814 MGI: 1891829 HomoloGene: 6872 GeneCards: RBX1 Gene

Gene Ontology
Molecular function	• ubiquitin-protein ligase activity • protein binding • zinc ion binding • ligase activity • NEDD8 ligase activity • metal ion binding
Cellular component	• nucleus • cytoplasm • cytosol • cytosol • SCF ubiquitin ligase complex • SCF ubiquitin ligase complex • VCB complex • Cul3-RING ubiquitin ligase complex • Cul4A-RING ubiquitin ligase complex • Cul4B-RING ubiquitin ligase complex
Biological process	• DNA repair • viral reproduction • protein ubiquitination • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process • proteasomal ubiquitin-dependent protein catabolic process • interspecies interaction between organisms • protein neddylation
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 22:
41.35 – 41.37 Mb

Chr 15:
81.3 – 81.31 Mb

PubMed search

[1]

[2]

RING-box protein 1 is a protein that in humans is encoded by the RBX1 gene.^[1]^[2]

This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over.^[3]

Interactions [link]

RBX1 has been shown to interact with CUL4A,^[2]^[4]^[5] CAND1,^[6]^[7] CUL1,^[2]^[4]^[6]^[7]^[8]^[9] CUL2,^[2]^[4] P70-S6 Kinase 1,^[10] DCUN1D1,^[7] CUL5^[2]^[11] and CUL7.^[4]

References [link]

^ Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG Jr, Elledge SJ, Conaway RC, Harper JW, Conaway JW (May 1999). "Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase". Science 284 (5414): 657–61. DOI:10.1126/science.284.5414.657. PMID 10213691.
^ ^a ^b ^c ^d ^e Ohta T, Michel JJ, Schottelius AJ, Xiong Y (May 1999). "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity". Mol Cell 3 (4): 535–41. DOI:10.1016/S1097-2765(00)80482-7. PMID 10230407.
^ "Entrez Gene: RBX1 ring-box 1". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9978.
^ ^a ^b ^c ^d Dias, Dora C; Dolios Georgia, Wang Rong, Pan Zhen-Qiang (Dec. 2002). "CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex". Proc. Natl. Acad. Sci. U.S.A. (United States) 99 (26): 16601–6. DOI:10.1073/pnas.252646399. ISSN 0027-8424. PMC 139190. PMID 12481031. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139190.
^ Guerrero-Santoro, Jennifer; Kapetanaki Maria G, Hsieh Ching L, Gorbachinsky Ilya, Levine Arthur S, Rapić-Otrin Vesna (Jul. 2008). "The cullin 4B-based UV-damaged DNA-binding protein ligase binds to UV-damaged chromatin and ubiquitinates histone H2A". Cancer Res. (United States) 68 (13): 5014–22. DOI:10.1158/0008-5472.CAN-07-6162. PMID 18593899.
^ ^a ^b Min, Kyoeng-Woo; Hwang Ji-Won, Lee Jong-Sik, Park Yoon, Tamura Taka-aki, Yoon Jong-Bok (May. 2003). "TIP120A associates with cullins and modulates ubiquitin ligase activity". J. Biol. Chem. (United States) 278 (18): 15905–10. DOI:10.1074/jbc.M213070200. ISSN 0021-9258. PMID 12609982.
^ ^a ^b ^c Kim, Alexander Y; Bommeljé Claire C, Lee Benjamin E, Yonekawa Yoshihiro, Choi Lydia, Morris Luc G, Huang Guochang, Kaufman Andrew, Ryan Russel J H, Hao Bing, Ramanathan Y, Singh Bhuvanesh (Nov. 2008). "SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation". J. Biol. Chem. (United States) 283 (48): 33211–20. DOI:10.1074/jbc.M804440200. ISSN 0021-9258. PMC 2586271. PMID 18826954. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2586271.
^ Kim, Jaehoon; Kim Jeong-Hoon, Lee Sung-Hak, Kim Do-Hyung, Kang Ho-Young, Bae Sung-Ho, Pan Zhen-Qiang, Seo Yeon-Soo (Jul. 2002). "The novel human DNA helicase hFBH1 is an F-box protein". J. Biol. Chem. (United States) 277 (27): 24530–7. DOI:10.1074/jbc.M201612200. ISSN 0021-9258. PMID 11956208.
^ Zheng, Ning; Schulman Brenda A, Song Langzhou, Miller Julie J, Jeffrey Philip D, Wang Ping, Chu Claire, Koepp Deanna M, Elledge Stephen J, Pagano Michele, Conaway Ronald C, Conaway Joan W, Harper J Wade, Pavletich Nikola P (Apr. 2002). "Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex". Nature (England) 416 (6882): 703–9. DOI:10.1038/416703a. ISSN 0028-0836. PMID 11961546.
^ Panasyuk, Ganna; Nemazanyy Ivan, Filonenko Valeriy, Gout Ivan (May. 2008). "Ribosomal protein S6 kinase 1 interacts with and is ubiquitinated by ubiquitin ligase ROC1". Biochem. Biophys. Res. Commun. (United States) 369 (2): 339–43. DOI:10.1016/j.bbrc.2008.02.016. PMID 18279656.
^ Duda, David M; Borg Laura A, Scott Daniel C, Hunt Harold W, Hammel Michal, Schulman Brenda A (Sep. 2008). "Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation". Cell (United States) 134 (6): 995–1006. DOI:10.1016/j.cell.2008.07.022. PMC 2628631. PMID 18805092. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2628631.

Further reading [link]

Skowyra D, Koepp DM, Kamura T, et al. (1999). "Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1.". Science 284 (5414): 662–5. DOI:10.1126/science.284.5414.662. PMID 10213692.
Tan P, Fuchs SY, Chen A, et al. (1999). "Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha.". Mol. Cell 3 (4): 527–33. DOI:10.1016/S1097-2765(00)80481-5. PMID 10230406.
Winston JT, Koepp DM, Zhu C, et al. (1999). "A family of mammalian F-box proteins.". Curr. Biol. 9 (20): 1180–2. DOI:10.1016/S0960-9822(00)80021-4. PMID 10531037.
Kamura T, Conrad MN, Yan Q, et al. (2000). "The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2.". Genes Dev. 13 (22): 2928–33. DOI:10.1101/gad.13.22.2928. PMC 317157. PMID 10579999. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=317157.
Dunham I, Shimizu N, Roe BA, et al. (1999). "The DNA sequence of human chromosome 22.". Nature 402 (6761): 489–95. DOI:10.1038/990031. PMID 10591208.
Périn J-P , Seddiqi N, Charbonnier F, et al. (2000). "Genomic organization and expression of the ubiquitin-proteasome complex-associated protein Rbx1/ROC1/Hrt1.". Cell. Mol. Biol. (Noisy-le-grand) 45 (8): 1131–7. PMID 10643962.
Read MA, Brownell JE, Gladysheva TB, et al. (2000). "Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha.". Mol. Cell. Biol. 20 (7): 2326–33. DOI:10.1128/MCB.20.7.2326-2333.2000. PMC 85397. PMID 10713156. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85397.
Furukawa M, Zhang Y, McCarville J, et al. (2000). "The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1.". Mol. Cell. Biol. 20 (21): 8185–97. DOI:10.1128/MCB.20.21.8185-8197.2000. PMC 86428. PMID 11027288. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=86428.
Maeda I, Ohta T, Koizumi H, Fukuda M (2001). "In vitro ubiquitination of cyclin D1 by ROC1-CUL1 and ROC1-CUL3.". FEBS Lett. 494 (3): 181–5. DOI:10.1016/S0014-5793(01)02343-2. PMID 11311237.
Lyapina S, Cope G, Shevchenko A, et al. (2001). "Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.". Science 292 (5520): 1382–5. DOI:10.1126/science.1059780. PMID 11337588.
Fukuchi M, Imamura T, Chiba T, et al. (2001). "Ligand-dependent degradation of Smad3 by a ubiquitin ligase complex of ROC1 and associated proteins.". Mol. Biol. Cell 12 (5): 1431–43. PMC 34595. PMID 11359933. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=34595.
Kamura T, Burian D, Yan Q, et al. (2001). "Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase.". J. Biol. Chem. 276 (32): 29748–53. DOI:10.1074/jbc.M103093200. PMID 11384984.
Wu K, Chen A, Tan P, Pan ZQ (2002). "The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34.". J. Biol. Chem. 277 (1): 516–27. DOI:10.1074/jbc.M108008200. PMID 11675391.
Gomes MD, Lecker SH, Jagoe RT, et al. (2002). "Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy.". Proc. Natl. Acad. Sci. U.S.A. 98 (25): 14440–5. DOI:10.1073/pnas.251541198. PMC 64700. PMID 11717410. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=64700.
Kim J, Kim JH, Lee SH, et al. (2002). "The novel human DNA helicase hFBH1 is an F-box protein.". J. Biol. Chem. 277 (27): 24530–7. DOI:10.1074/jbc.M201612200. PMID 11956208.
Zheng N, Schulman BA, Song L, et al. (2002). "Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex.". Nature 416 (6882): 703–9. DOI:10.1038/416703a. PMID 11961546.
Yoshida Y, Chiba T, Tokunaga F, et al. (2002). "E3 ubiquitin ligase that recognizes sugar chains.". Nature 418 (6896): 438–42. DOI:10.1038/nature00890. PMID 12140560.
Brower CS, Sato S, Tomomori-Sato C, et al. (2002). "Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase.". Proc. Natl. Acad. Sci. U.S.A. 99 (16): 10353–8. DOI:10.1073/pnas.162424199. PMC 124918. PMID 12149480. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=124918.