Rbpj

Recombination signal binding protein for immunoglobulin kappa J region

PDB rendering based on 2f8x.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2F8X, 3NBN, 3V79

Identifiers

Symbols

RBPJ; CBF1; IGKJRB; IGKJRB1; KBF2; RBP-J; RBPJK; RBPSUH; SUH; csl

External IDs

OMIM: 147183 MGI: 96522 HomoloGene: 7511 GeneCards: RBPJ Gene

Gene Ontology
Molecular function	• recombinase activity • RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription • RNA polymerase II repressing transcription factor binding • DNA binding • sequence-specific DNA binding transcription factor activity • protein binding
Cellular component	• MAML1-RBP-Jkappa- ICN1 complex • nucleus • nucleoplasm • nucleolus • cytoplasm
Biological process	• DNA recombination • regulation of transcription, DNA-dependent • Notch signaling pathway • positive regulation of transcription of Notch receptor target • gene expression • negative regulation of transcription, DNA-dependent
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 4:
26.17 – 26.44 Mb

Chr 5:
53.95 – 54.05 Mb

PubMed search

[1]

[2]

Recombining binding protein suppressor of hairless is a protein that in humans is encoded by the RBPJ gene.^[1]^[2]^[3]

RBPJ,^[4] also named as CBF1, is a human homolog for the Drosophila gene Suppressor of Hairless. Its promoter region is classically used to demonstrate Notch1 signaling.^[5]

1 Interactions
2 References
3 Further reading
4 External links

Interactions [link]

RBPJ has been shown to interact with PCAF,^[6] Nuclear receptor co-repressor 2,^[7]^[8] SNW1,^[9]^[10] NOTCH1^[11]^[12] and SND1.^[9]^[13]

References [link]

^ Amakawa R, Jing W, Ozawa K, Matsunami N, Hamaguchi Y, Matsuda F, Kawaichi M, Honjo T (October 1993). "Human Jk recombination signal binding protein gene (IGKJRB): comparison with its mouse homologue". Genomics 17 (2): 306–15. DOI:10.1006/geno.1993.1326. PMID 8406481.
^ Tang X, Saito-Ohara F, Song J, Koga C, Ugai H, Murakami H, Ikeuchi T, Yokoyama KK (November 1997). "Assignment of the human gene for KBF2/RBP-Jk to chromosome 9p12-13 and 9q13 by fluorescence in situ hybridization". Jpn J Hum Genet 42 (2): 337–41. DOI:10.1007/BF02766956. PMID 9290259.
^ "Entrez Gene: RBPJ recombination signal binding protein for immunoglobulin kappa J region". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3516.
^ "Human Genome Organization: RBPJ Report". https://www.genenames.org/data/hgnc_data.php?hgnc_id=5724.
^ Hsieh JJ, Henkel T, Salmon P, Robey E, Peterson MG, Hayward SD (1996). "Truncated mammalian Notch1 activates CBF1/RBPJk-repressed genes by a mechanism resembling that of Epstein-Barr virus EBNA2.". Molecular and Cellular Biology 16 (3): 952–959. PMC 231077. PMID 8622698. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=231077.
^ Kurooka, H; Honjo T (June 2000). "Functional interaction between the mouse notch1 intracellular region and histone acetyltransferases PCAF and GCN5". J. Biol. Chem. (UNITED STATES) 275 (22): 17211–20. DOI:10.1074/jbc.M000909200. ISSN 0021-9258. PMID 10747963.
^ Beatus, P; Lundkvist J, Oberg C, Pedersen K, Lendahl U (June 2001). "The origin of the ankyrin repeat region in Notch intracellular domains is critical for regulation of HES promoter activity". Mech. Dev. (Ireland) 104 (1–2): 3–20. DOI:10.1016/S0925-4773(01)00373-2. ISSN 0925-4773. PMID 11404076.
^ Zhou, S; Hayward S D (September 2001). "Nuclear localization of CBF1 is regulated by interactions with the SMRT corepressor complex". Mol. Cell. Biol. (United States) 21 (18): 6222–32. DOI:10.1128/MCB.21.18.6222-6232.2001. ISSN 0270-7306. PMC 87339. PMID 11509665. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=87339.
^ ^a ^b Zhou, S; Fujimuro M, Hsieh J J, Chen L, Hayward S D (February 2000). "A role for SKIP in EBNA2 activation of CBF1-repressed promoters". J. Virol. (UNITED STATES) 74 (4): 1939–47. DOI:10.1128/JVI.74.4.1939-1947.2000. ISSN 0022-538X. PMC 111672. PMID 10644367. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=111672.
^ Zhou, S; Fujimuro M, Hsieh J J, Chen L, Miyamoto A, Weinmaster G, Hayward S D (April 2000). "SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function". Mol. Cell. Biol. (UNITED STATES) 20 (7): 2400–10. DOI:10.1128/MCB.20.7.2400-2410.2000. ISSN 0270-7306. PMC 85419. PMID 10713164. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85419.
^ Nam, Yunsun; Weng Andrew P, Aster Jon C, Blacklow Stephen C (June 2003). "Structural requirements for assembly of the CSL.intracellular Notch1.Mastermind-like 1 transcriptional activation complex". J. Biol. Chem. (United States) 278 (23): 21232–9. DOI:10.1074/jbc.M301567200. ISSN 0021-9258. PMID 12644465.
^ Aster, J C; Robertson E S, Hasserjian R P, Turner J R, Kieff E, Sklar J (April 1997). "Oncogenic forms of NOTCH1 lacking either the primary binding site for RBP-Jkappa or nuclear localization sequences retain the ability to associate with RBP-Jkappa and activate transcription". J. Biol. Chem. (UNITED STATES) 272 (17): 11336–43. DOI:10.1074/jbc.272.17.11336. ISSN 0021-9258. PMID 9111040.
^ Hsieh, J J; Zhou S, Chen L, Young D B, Hayward S D (January 1999). "CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 96 (1): 23–8. DOI:10.1073/pnas.96.1.23. ISSN 0027-8424. PMC 15086. PMID 9874765. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=15086.

Further reading [link]

Oka C, Kawaichi M (1995). "V(D)J recombination of immunoglobulin genes". Adv. Biophys. 31: 163–80. DOI:10.1016/0065-227X(95)99390-B. PMID 7625272.
Matsunami N, Hamaguchi Y, Yamamoto Y, et al. (1990). "A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif". Nature 342 (6252): 934–7. DOI:10.1038/342934a0. PMID 2556644.
Tong X, Drapkin R, Yalamanchili R, et al. (1995). "The Epstein-Barr virus nuclear protein 2 acidic domain forms a complex with a novel cellular coactivator that can interact with TFIIE". Mol. Cell. Biol. 15 (9): 4735–44. PMC 230717. PMID 7651391. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=230717.
Zhao B, Marshall DR, Sample CE (1996). "A conserved domain of the Epstein-Barr virus nuclear antigens 3A and 3C binds to a discrete domain of Jkappa". J. Virol. 70 (7): 4228–36. PMC 190353. PMID 8676443. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=190353.
Tamura K, Taniguchi Y, Minoguchi S, et al. (1997). "Physical interaction between a novel domain of the receptor Notch and the transcription factor RBP-J kappa/Su(H)". Curr. Biol. 5 (12): 1416–23. DOI:10.1016/S0960-9822(95)00279-X. PMID 8749394.
Gress TM, Müller-Pillasch F, Geng M, et al. (1996). "A pancreatic cancer-specific expression profile". Oncogene 13 (8): 1819–30. PMID 8895530.
Hsieh JJ, Nofziger DE, Weinmaster G, Hayward SD (1997). "Epstein-Barr virus immortalization: Notch2 interacts with CBF1 and blocks differentiation". J. Virol. 71 (3): 1938–45. PMC 191274. PMID 9032325. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=191274.
Aster JC, Robertson ES, Hasserjian RP, et al. (1997). "Oncogenic forms of NOTCH1 lacking either the primary binding site for RBP-Jkappa or nuclear localization sequences retain the ability to associate with RBP-Jkappa and activate transcription". J. Biol. Chem. 272 (17): 11336–43. DOI:10.1074/jbc.272.17.11336. PMID 9111040.
Kao HY, Ordentlich P, Koyano-Nakagawa N, et al. (1998). "A histone deacetylase corepressor complex regulates the Notch signal transduction pathway". Genes Dev. 12 (15): 2269–77. DOI:10.1101/gad.12.15.2269. PMC 317043. PMID 9694793. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=317043.
Hsieh JJ, Zhou S, Chen L, et al. (1999). "CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex". Proc. Natl. Acad. Sci. U.S.A. 96 (1): 23–8. DOI:10.1073/pnas.96.1.23. PMC 15086. PMID 9874765. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=15086.
Scanlan MJ, Gordan JD, Williamson B, et al. (1999). "Antigens recognized by autologous antibody in patients with renal-cell carcinoma". Int. J. Cancer 83 (4): 456–64. DOI:10.1002/(SICI)1097-0215(19991112)83:4<456::AID-IJC4>3.0.CO;2-5. PMID 10508479.
Callahan J, Aster J, Sklar J, et al. (2000). "Intracellular forms of human NOTCH1 interact at distinctly different levels with RBP-jkappa in human B and T cells". Leukemia 14 (1): 84–92. DOI:10.1038/sj.leu.2401630. PMID 10637481.
Smith PR, de Jesus O, Turner D, et al. (2000). "Structure and coding content of CST (BART) family RNAs of Epstein-Barr virus". J. Virol. 74 (7): 3082–92. DOI:10.1128/JVI.74.7.3082-3092.2000. PMC 111807. PMID 10708423. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=111807.
Zhou S, Fujimuro M, Hsieh JJ, et al. (2000). "SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function". Mol. Cell. Biol. 20 (7): 2400–10. DOI:10.1128/MCB.20.7.2400-2410.2000. PMC 85419. PMID 10713164. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85419.
Kurooka H, Honjo T (2000). "Functional interaction between the mouse notch1 intracellular region and histone acetyltransferases PCAF and GCN5". J. Biol. Chem. 275 (22): 17211–20. DOI:10.1074/jbc.M000909200. PMID 10747963.
Beatus P, Lundkvist J, Oberg C, et al. (2001). "The origin of the ankyrin repeat region in Notch intracellular domains is critical for regulation of HES promoter activity". Mech. Dev. 104 (1–2): 3–20. DOI:10.1016/S0925-4773(01)00373-2. PMID 11404076.
Zhou S, Hayward SD (2001). "Nuclear localization of CBF1 is regulated by interactions with the SMRT corepressor complex". Mol. Cell. Biol. 21 (18): 6222–32. DOI:10.1128/MCB.21.18.6222-6232.2001. PMC 87339. PMID 11509665. //www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=87339.

PDB gallery

2f8x: Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence

External links [link]

RBPJ+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

Transcription factors and intracellular receptors

(1) Basic domains

(1.1) Basic leucine zipper (bZIP)	Activating transcription factor (AATF, 1, 2, 3, 4, 5, 6, 7) · AP-1 (c-Fos, FOSB, FOSL1, FOSL2, JDP2, c-Jun, JUNB, JUND) · BACH (1, 2) · BATF · BLZF1 · C/EBP (α, β, γ, δ, ε, ζ) · CREB (1, 3, L1) · CREM · DBP · DDIT3 · GABPA · HLF · MAF (B, F, G, K) · NFE (2, L1, L2, L3) · NFIL3 · NRL · NRF (1, 2, 3) · XBP1

(1.2) Basic helix-loop-helix (bHLH)	ATOH1 · AhR · AHRR · ARNT · ASCL1 · BHLH (2, 9) · BMAL (ARNTL, ARNTL2) · CLOCK · EPAS1 · FIGLA · HAND (1, 2) · HES (5, 6) · HEY (1, 2, L) · HES1 · HIF (1A, 3A) · ID (1, 2, 3, 4) · LYL1 · MESP2 · MXD4 · MYCL1 · MYCN · Myogenic regulatory factors (MyoD, Myogenin, MYF5, MYF6) · Neurogenins (1, 2, 3) · NeuroD (1, 2) · NPAS (1, 2, 3) · OLIG (1, 2) · Pho4 · Scleraxis · SIM (1, 2) · TAL (1, 2) · Twist · USF1

(1.3) bHLH-ZIP	AP-4 · MAX · MITF · MNT · MLX · MXI1 · Myc · SREBP (1, 2)

(1.4) NF-1	NFI (A, B, C, X) · SMAD (R-SMAD (1, 2, 3, 5, 9) - I-SMAD (6, 7) - 4)

(1.5) RF-X	RFX (1, 2, 3, 4, 5, 6, ANK)

(1.6) Basic helix-span-helix (bHSH)	AP-2 (α, β, γ, δ, ε)

(2) Zinc finger DNA-binding domains

(2.1) Nuclear receptor (Cys₄)	subfamily 1 (Thyroid hormone (α, β), CAR, FXR, LXR (α, β), PPAR (α, β/δ, γ), PXR, RAR (α, β, γ), ROR (α, β, γ), Rev-ErbA (α, β), VDR) subfamily 2 (COUP-TF (I, II), Ear-2, HNF4 (α, γ), PNR, RXR (α, β, γ), Testicular receptor (2, 4), TLX) subfamily 3 (Steroid hormone (Androgen, Estrogen (α, β), Glucocorticoid, Mineralocorticoid, Progesterone), Estrogen related (α, β, γ)) subfamily 4 NUR (NGFIB, NOR1, NURR1) · subfamily 5 (LRH-1, SF1) · subfamily 6 (GCNF) · subfamily 0 (DAX1, SHP)

(2.2) Other Cys₄	GATA (1, 2, 3, 4, 5, 6) · MTA (1, 2, 3) · TRPS1

(2.3) Cys₂His₂	General transcription factors (TFIIA, TFIIB, TFIID, TFIIE (1, 2), TFIIF (1, 2), TFIIH (1, 2, 4, 2I, 3A, 3C1, 3C2)) ATBF1 · BCL (6, 11A, 11B) · CTCF · E4F1 · EGR (1, 2, 3, 4) · ERV3 · GFI1 · GLI-Krüppel family (1, 2, 3, REST, S2, YY1) · HIC (1, 2) · HIVEP (1, 2, 3) · IKZF (1, 2, 3) · ILF (2, 3) · KLF (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17) · MTF1 · MYT1 · OSR1 · PRDM9 · SALL (1, 2, 3, 4) · SP (1, 2, 4, 7, 8) · TSHZ3 · WT1 · Zbtb7 (7A, 7B) · ZBTB (11, 16, 17, 20, 32, 33, 40) · zinc finger (3, 7, 9, 10, 19, 22, 24, 33B, 34, 35, 41, 43, 44, 51, 74, 143, 146, 148, 165, 202, 217, 219, 238, 239, 259, 267, 268, 281, 295, 300, 318, 330, 346, 350, 365, 366, 384, 423, 451, 452, 471, 593, 638, 644, 649, 655)

(2.4) Cys₆	HIVEP1

(2.5) Alternating composition	AIRE · DIDO1 · GRLF1 · ING (1, 2, 4) · JARID (1A, 1B, 1C, 1D, 2) · JMJD1B

(3) Helix-turn-helix domains

(3.1) Homeodomain	ARX · CDX (1, 2) · CRX · CUTL1 · DBX (1, 2) · DLX (3, 4, 5) · EMX (1, 2) · EN (1, 2) · FHL (1, 2, 3) · HESX1 · HHEX · HLX · Homeobox (A1, A2, A3, A4, A5, A7, A9, A10, A11, A13, B1, B2, B3, B4, B5, B6, B7, B8, B9, B13, C4, C5, C6, C8, C9, C10, C11, C12, C13, D1, D3, D4, D8, D9, D10, D11, D12, D13) · HOPX · IRX (1, 2, 3, 4, 5, 6, MKX) · LMX (1A, 1B) · MEIS (1, 2) · MEOX2 · MNX1 · MSX (1, 2) · NANOG · NKX (2-1, 2-2, 2-3, 2-5, 3-1, 3-2, 6-1, 6-2) · NOBOX · PBX (1, 2, 3) · PHF (1, 3, 6, 8, 10, 16, 17, 20, 21A) · PHOX (2A, 2B) · PITX (1, 2, 3) · POU domain (PIT-1, BRN-3: A, B, C, Octamer transcription factor: 1, 2, 3/4, 6, 7, 11) · OTX (1, 2) · PDX1 · SATB2 · SHOX2 · SIX (1, 2, 3, 4, 5) · VAX1 · ZEB (1, 2)

(3.2) Paired box	PAX (1, 2, 3, 4, 5, 6, 7, 8, 9)

(3.3) Fork head / winged helix	E2F (1, 2, 3, 4, 5) · FOX proteins (A1, A2, A3, C1, C2, D3, D4, E1, E3, F1, G1, H1, I1, J1, J2, K1, K2, L2, M1, N1, N3, O1, O3, O4, P1, P2, P3, P4)

(3.4) Heat Shock Factors	HSF (1, 2, 4)

(3.5) Tryptophan clusters	ELF (2, 4, 5) · EGF · ELK (1, 3, 4) · ERF · ETS (1, 2, ERG, SPIB) · ETV (1, 4, 5, 6) · FLI1 · Interferon regulatory factors (1, 2, 3, 4, 5, 6, 7, 8) · MYB · MYBL2

(3.6) TEA domain	transcriptional enhancer factor (1, 2, 3, 4)

(4) β-Scaffold factors with minor groove contacts

(4.1) Rel homology region	NF-κB (NFKB1, NFKB2, REL, RELA, RELB) · NFAT (C1, C2, C3, C4, 5)

(4.2) STAT	STAT (1, 2, 3, 4, 5, 6)

(4.3) p53	p53 · TBX (1, 2, 3, 5, 19, 21, 22, Brachyury, TBR1, TBR2) · TP63

(4.4) MADS box	Mef2 (A, B, C, D) · SRF

(4.6) TATA binding proteins	TBP · TBPL1

(4.7) High-mobility group	BBX · HMGB (1, 2, 3, 4) · HMGN (1, 2, 3, 4) · HNF (1A, 1B) · LEF1 · SOX (1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 18, 21) · SRY · SSRP1 · TCF (3, 4) · TOX (1, 2, 3, 4)

(4.9) Grainyhead	TFCP2

(4.10) Cold-shock domain	CSDA, YBX1

(4.11) Runt	CBF (CBFA2T2, CBFA2T3, RUNX1, RUNX2, RUNX3, RUNX1T1)

(0) Other transcription factors

(0.2) HMGI(Y)	HMGA (1, 2) · HBP1

(0.3) Pocket domain	Rb · RBL1 · RBL2

(0.5) AP-2/EREBP-related factors	Apetala 2 · EREBP · B3

(0.6) Miscellaneous	ARID (1A, 1B, 2, 3A, 3B, 4A) · CAP · IFI (16, 35) · MLL (2, 3, T1) · MNDA · NFY (A, B, C) · Rho/Sigma

see also transcription factor/coregulator deficiencies
B bsyn: dna (repl, cycl, reco, repr) · tscr (fact, tcrg, nucl, rnat, rept, ptts) · tltn (risu, pttl, nexn) · dnab, rnab/runp · stru (domn, 1°, 2°, 3°, 4°)

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