RAD18
E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene.
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is a ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Animal models
Model organisms have been used in the study of RAD18 function. A conditional knockout mouse line, called Rad18tm1a(EUCOMM)Wtsi, was generated as part of the EUCOMM program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute. Mice lacking Rad18 had no significant defects in viability or fertility, therefore male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.
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by Ruth Moody
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by Ruth Moody
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by Ruth Moody
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by Ruth Moody
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by Ruth Moody
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by Ruth Moody
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by Ruth Moody
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by Ruth Moody
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by Ruth Moody
The Garden
by: Ruth MoodyO light shine down on me You know what I need Shine down on me Shine down on the garden Shine down on the garden
Sweet earth alive under me You know what I need Cradle me like a seed As I lay in the garden As I lay in the garden
O wise and beautiful tree Standing high over me Oh the things you have seen Tell me your story in the garden Tell it to me in the garden
And tell me How long have we slept How long have we wept There's work to be done
Sky above So vast and so deep You know what I need Rain down on me Rain down on the garden Rain down on the garden
O light surrounding me Sweet mystery In everything I see Come and find me in the garden Come and find me in the garden