Program and System Information Protocol

The Program and System Information Protocol (PSIP) is the MPEG and privately defined program-specific information originally defined by General Instrument for the DigiCipher 2 system and later extended for the ATSC digital television system for carrying metadata about each channel in the broadcast MPEG transport stream of a television station and for publishing information about television programs so that viewers can select what to watch by title and description.

What PSIP does

PSIP defines virtual channels and content ratings, as well as electronic program guides with titles and (optionally) descriptions to be decoded and displayed by the ATSC tuner.

PSIP can also send:

PSIP is defined in ATSC standard A/65, the most recent revision of which is A/65:2013, published in 2013. A/69 is a recommended practice for implementing PSIP in a television station.

PSIP1

PC4 and SFRS1 interacting protein 1, also known as lens epithelium-derived growth factor (LEDGF/p75), dense fine speckles 70kD protein (DFS 70) or transcriptional coactivator p75/p52, is a protein that in humans is encoded by the PSIP1 gene.

Function

PSIP1 has not been clearly linked to a specific cellular mechanism. The term LEDGF/p75 (Lens epithelium-derived growth factor) has entered common usage based on the initial characterization of PSIP1, however this is a misnomer, as the protein is present in most tissues and has no direct role in the development of lens epithelium. LEDGF/p75, a transcription coactivator, gained prominence as a host factor that assists HIV integration and is probably the only integrase interactor whose knock-down severely affects the HIV integration levels. The interaction between HIV integrase and human LEDGF/p75 is a promising target for anti-HIV drug discovery.

Structure

LEDGF/p75 is a 60kDa, 530-amino-acid-long protein. The N-terminal portion of the protein consists of a PWWP domain, a nuclear localization sequence, and two copies of the AT-hook DNA binding motif. The C-terminal portion of LEDGF/p75 contains a structure termed the integrase-binding domain, which interacts with lentiviral integrase proteins as well as numerous cellular proteins. The N-terminal portion interacts strongly with chromatin, making LEDGF/p75 a constitutively nuclear protein. An isoform of the protein, LEDGF/p52, is produced by alternative splicing. LEDGF/p52 shares the N-terminal 325 amino acids of LEDGF/p75 but lacks the integrase-binding domain.