PNP

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Purine nucleoside phosphorylase

Purine nucleoside phosphorylase also known as PNPase and inosine phosphorylase is an enzyme that in humans is encoded by the NP gene.

Function

Purine nucleoside phosphorylase is an enzyme involved in purine metabolism. PNP metabolizes inosine into hypoxanthine and guanosine into guanine, in each case creating ribose phosphate. Note: adenosine is first metabolized to inosine via the enzyme adenosine deaminase.

Nucleoside phosphorylase is an enzyme which cleaves a nucleoside by phosphorylating the ribose to produce a nucleobase and ribose 1 phosphate. It is one enzyme of the nucleotide salvage pathways. These pathways allow the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). Often the de novo pathway is interrupted as a result of chemotherapy drugs such as methotrexate or aminopterin.

All salvage pathway enzymes require a high energy phosphate donor such as ATP or PRPP.

Bipolar junction transistor

A bipolar junction transistor (bipolar transistor or BJT) is a type of transistor that uses both electron and hole charge carriers. In contrast, unipolar transistors, such as field-effect transistors, only use one kind of charge carrier. For their operation, BJTs use two junctions between two semiconductor types, n-type and p-type.

BJTs are manufactured in two types, NPN and PNP, and are available as individual components, or fabricated in integrated circuits, often in large numbers. The basic function of a BJT is to amplify current. This allows BJTs to be used as amplifiers or switches, giving them wide applicability in electronic equipment, including computers, televisions, mobile phones, audio amplifiers, industrial control, and radio transmitters.

Note on current direction

By convention the direction of current on diagrams is shown as the direction that a positive charge would move. This is called conventional current. However, current in metal conductors is due to the flow of electrons which, because they carry a negative charge, move in the opposite direction to conventional current. On the other hand, inside a bipolar transistor, currents can be composed of both positively charged holes and negatively charged electrons. In this article, current arrows are shown in the conventional direction, but labels for the movement of holes and electrons show their actual direction inside the transistor.