Pleckstrin homology domain
Pleckstrin homology domain (PH domain) is a protein domain of approximately 120 amino acids that occurs in a wide range of proteins involved in intracellular signaling or as constituents of the cytoskeleton.
This domain can bind Phosphatidylinositol lipids within biological membranes (such as Phosphatidylinositol (3,4,5)-trisphosphate and phosphatidylinositol (4,5)-bisphosphate), and proteins such as the βγ-subunits of heterotrimeric G proteins, and protein kinase C. Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways.
Lipid binding specificity
Individual PH domains possess specificities for phosphoinositides phosphorylated at different sites within the inositol ring, e.g., some bind phosphatidylinositol (4,5)-bisphosphate but not phosphatidylinositol (3,4,5)-trisphosphate or phosphatidylinositol (3,4)-bisphosphate, while others may possess the requisite affinity. This is important because it makes the recruitment of different PH domain containing proteins sensitive to the activities of enzymes that either phosphorylate or dephosphorylate these sites on the inositol ring, such as phosphoinositide 3-kinase or PTEN, respectively. Thus, such enzymes exert a part of their effect on cell function by modulating the localization of downstream signaling proteins that possess PH domains that are capable of binding their phospholipid products.
Podcasts:
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by Joe Cocker
Five Women
by: Joe CockerIt took five women to getcha off of my mind
It took five months, a plenty good wasted time
But it just took five minutes when I saw your face again
To fall in love all over, I guess with you, I'll never win
March was cold in more ways than one
I went down to the nightclub lookin' for the sun
Lookin' for the light that could brighten up a day
That's been darker than a hole since you went away
April usually brings showers
This time it was a hurtin' kind
This woman said she wanted my baby
I told her I'd have to be deaf, dumb, and blind
To fall in love with someone, someone that I barely knew
The child would never really have a father
Oh and I'd be stuck, stuck on u
It took five women to getcha off of my mind
Get off my mind, baby
Fallin' in love all over, you I'll never win
May was her name and she was jumpin'
Jumpin' from plane to plane
She was an actress or model or somethin'
Can't even remember her first name
June brought an Easter bunny
Needless to say, that girl was late
Told a million stories to a busy-body of our one and only date
July was a heartbreak, extraordinary
I met this woman whose kiss was somethin' scary
It felt so much, so much like yours
That only made me, made me want you more
She's gone, she's gone but you're still here
I want you very [Incomprehensible]
It took five women to getcha off of my mind
It took five months, a plenty good wasted time
Oh, but it just took five minutes when I saw your face again