Prostate cancer antigen 3 (PCA3, also referred to as DD3) is a gene that expresses a non-coding RNA. PCA3 is only expressed in human prostate tissue, and the gene is highly overexpressed in prostate cancer. Because of its restricted expression profile, the PCA3 RNA is useful as a tumor marker.
The most frequently used biomarker for prostate cancer today is the serum level of prostate-specific antigen (PSA), or derived measurements. However, since PSA is prostate-specific but not cancer-specific, it is an imperfect biomarker. For example, PSA can increase in older men with benign prostatic hyperplasia. Several new biomarkers are being investigated to improve the diagnosis of prostate cancer. Some of these can be measured in urine samples, and it is possible that a combination of several urinary biomarkers will replace PSA in the future.
Compared to serum PSA, PCA3 has a lower sensitivity but a higher specificity and a better positive and negative predictive value. It is independent of prostate volume, whereas PSA is not. It should be measured in the first portion of urine after prostate massage with digital rectal examination.
Yo 'Pac yo, I heard you got beat up by the police
Got a big fat lawsuit and everything
Niggaz just wanna know
if you still gon' be on some crooked-ass sheeeit
[2Pac]
Please tell me what's a nigga to do, and it's true
Ain't nuttin new, so I do, what I can to get through
Now first they had me trapped and now I'm pissed
A loaded AK-47 lay under my head so I don't trip
One motherfucker from the Underground
And Big Stretch buckin niggaz if they fuck around
Yo why I got beef with police?
Ain't that a bitch that motherfuckers got a beef with me
They make it hard for me to sleep
I wake up at the slightest peep, and my sheets are 3 feet deep
I guess it's hard for you to see
But now I'm pointin the finger at police
instead of them motherfuckers blamin me
I got the right to bear a pistol
And when the punk motherfuckers get to trippin I got shit too
And maybe then you'll see the truth (hell yeah)
But until then, I gotta do what I do
and stay a crooked nigga too
[Chorus: Raphael Saadiq]
I've got to do, what I'm gon' do
I'm gon' say what I'm gon' say
I'm gon' live how I live - how else you want a nigga to live?
I'm gon' do, what I do
I'm gon' say, what I say
I'm gon' live how I live - how do you want a nigga to live?
[2Pac]
Y'know you really can't say that ya blame niggaz
Fuck bein tame, set aflame, time to aim triggers
2Pac'll spark a revolution, fuck the Constitution
I want my bucks for restitution
This time you got a bigger problem
Time to face the niggaz from South Central, Oakland, Brooklyn and Harlem
And we ain't shootin at each other
That's my motherfuckin brother, so Dave Duke, run for cover
And all the bitches from the Klan
Come feel the wrath of a black man that doesn't smoke crack and
I don't drink St. Ides (fuck that!)
Genuine Draft, ganja ganja, and my fuckin tec-9
They know they scared to see us sober
Stop drinkin King Cobra, and niggaz'll take the world over
It's all up to you (up to you)
Blame the Korean, blame the jew, or be a crooked nigga too
[Chorus]
[2Pac]
Aiyyo! Why me? Play like Jasmine Guy and try me
I'll be damned if I die, come look at the rage in my eyes G
They got my homies in a jail cell
And it's the Rebel and the Devil, and one of us is goin to Hell
I got the whole place covered, with loc'd out brothers
And nuttin but love for each other
So motherfucker make a motion
I give a fuck, slice you up, and throw your ass in the ocean
Temperatures drop; see it's cool to shoot a nigga
but they hate it when we pop the cops
That's when they gettin petrol
You better watch your step or you'll be left on death row
But I learn to look ahead of me
Stay strapped watch your back keep your eyes on the enemy
We blowin up precincts and OOOH
You can't fuck with the crew, of crooked nigga too
[Chorus]
[Raphael Saadiq]
It's the coldest town from here to Georgia
(I'm a crooked nigga too)
It's the coldest town from here to Georgia
It's the coldest town from here to Georgia
(I'm a crooked.. crooked nigga too)
It's the coldest town from here to Georgia
Y'all gon' stop fuckin with me
[Chorus - repeat to fade]