Polyclonal antibodies

Polyclonal antibodies (pAbs) are antibodies that are secreted by different B cell lineages within the body (whereas monoclonal antibodies come from a single cell lineage). They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.

Production

These antibodies are typically produced by inoculation of a suitable mammal, such as a mouse, rabbit or goat. Larger mammals are often preferred as the amount of serum that can be collected is greater. An antigen is injected into the mammal. This induces the B-lymphocytes to produce IgG immunoglobulins specific for the antigen. This polyclonal IgG is purified from the mammal’s serum.

By contrast, monoclonal antibodies are derived from a single cell line.

Many methodologies exist for polyclonal antibody production in laboratory animals. Institutional guidelines governing animal use and procedures relating to these methodologies are generally oriented around humane considerations and appropriate conduct for adjuvant (agents which modify the effect of other agents while having few if any direct effects when given by themselves) use. This includes adjuvant selection, routes and sites of administration, injection volumes per site and number of sites per animal. Institutional policies generally include allowable volumes of blood per collection and safety precautions including appropriate restraint and sedation or anesthesia of animals for injury prevention to animals or personnel.

PAB

PAB may refer to:

TRNA (guanine10-N2)-dimethyltransferase

TRNA (guanine¹⁰-N²)-dimethyltransferase (EC 2.1.1.213, PAB1283, N(2),N(2)-dimethylguanosine tRNA methyltransferase, Trm-G10, PabTrm-G10, PabTrm-m2 2G10 enzyme) is an enzyme with system name S-adenosyl-L-methionine:tRNA (guanine¹⁰-N²)-dimethyltransferase. This enzyme catalyses the following chemical reaction

References

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