Nucleoid
The nucleoid (meaning nucleus-like) is an irregularly shaped region within the cell of a prokaryote that contains all or most of the genetic material, called genophore. In contrast to the nucleus of a eukaryotic cell, it is not surrounded by a nuclear membrane. The genome of prokaryotic organisms generally is a circular, double-stranded piece of DNA, of which multiple copies may exist at any time. The length of a genome widely varies, but generally is at least a few million base pairs. As in all cellular organisms, length of the DNA molecules of bacterial and archaeal chromosomes is very large compared to the dimensions of the cell, and the genomic DNA molecules must be compacted to fit.
Visualization
The nucleoid can be clearly visualized on an electron micrograph at high magnification, where, although its appearance may differ, it is clearly visible against the cytosol. Sometimes even strands of what is thought to be DNA are visible. By staining with the Feulgen stain, which specifically stains DNA, the nucleoid can also be seen under a light microscope. The DNA-intercalating stains DAPI and ethidium bromide are widely used for fluorescence microscopy of nucleoids.
Podcasts:
-
by Nellie McKay
-
by Cardiacs
-
by Delfinai
-
by Waitresses
No Equality
by: Nellie McKayThere's no equality here
There's no equality anywhere
And every failing face
Is quickly replaced
By one you can't lose
It's an illusion
I'll even do revolution
That's never meant to occur
Won't happen to her
Won't happen to you
And you let me down slowly
Let me down slow and you turned me around
Let me down easy
Let yourself go, now you're goin' to town
Baby, baby, can't you see
What this hypocrisy is doin' to me?
We should've kicked over the ladders
From the start
You used to be my lover
You used to be my soul, soul brother
I used to believe
That you could conceive
Conceive with my love
But you let me down slowly
You let me down slow and you turned me around
Let me down easy
Let yourself go, now you're goin' to drown
Baby, baby, can't you see
What this absurdity is doin' to me?
We should've kicked over the ladders
From the start
There's no equality
Nobody knows the trouble I've seen
No one supposes just how mean I can be
I play the victim and I really care
And all I've ever wanted is
Everything I'm gonna have
There's no equality here
There's no equality on earth I fear
There are so many [Incomprehensible]
The boss and the border
It's me or it's you
That's why I let you down slowly
Let you down slow and I turned you around
Let you down easy
Let myself go, now we're both set to drown
Baby, baby, can't you see
What this futility is doin' to me?
We should've kicked over the ladders
From the start