No. 53 Squadron of the Royal Flying Corps was formed at Catterick on 15 May 1916. Originally intended to be a training squadron, it was sent to France to operate reconnaissance in December that year. The squadron was equipped with the B.E.2e—swapped for the R.E.8 in April 1917. It returned the UK in March 1919 to Old Sarum where it was disbanded on 25 October 1919.
The squadron reformed on 28 June 1937 at RAF Farnborough with the Hawker Hector for Army Cooperation; specialising in night reconnaissance.
The squadron was given the Bristol Blenheim light bomber in January 1939 and moved to France in September. Following the German attack on France in May 1940, the squadron returned to the UK. From here it undertook bombing and reconnaissance missions.