Replication protein A

Replication protein A (RPA) is a protein that binds to single-stranded DNA in eukaryotic cells. During DNA replication, RPA prevents single-stranded DNA (ssDNA) from winding back on itself or from forming secondary structures. This keeps DNA unwound for the polymerase to replicate it. RPA also binds to ssDNA during the initial phase of homologous recombination, an important process in DNA repair and prophase I of meiosis. Hypersensitivity to DNA damaging agents can be caused by mutations in the RPA gene. Like its role in DNA replication, this keeps ssDNA from binding to itself (self-complementizing) so that the resulting nucleoprotein filament can then be bound by Rad51 and its cofactors. RPA also binds to DNA during the Nucleotide Excision Repair process. This binding stabilizes the repair complex during the repair process. A bacterial homolog is called single-strand binding protein (SSB).

Structure

RPA is a heterotrimer, composed of the subunits RPA1 (70kDa subunit), RPA2 (32kDa subunit) and RPA3 (14kDa subunit). The three RPA subunits contain OB-folds (oligonucleotide/oligosaccharide binding).

Representation of the People (Equal Franchise) Act 1928

The Representation of the People (Equal Franchise) Act 1928 was an Act of the Parliament of the United Kingdom. This act expanded on the Representation of the People Act 1918 which had given some women the vote in Parliamentary elections for the first time after World War I. The 1928 Act widened suffrage by giving women electoral equality with men. It gave the vote to all women over 21 years old, regardless of property ownership. Prior to this act only women over 30 who met minimum property qualifications could vote. Similar provision was made for the Parliament of Northern Ireland by the Representation of the People Act (Northern Ireland) 1928 (18 & 19 Geo V, Ch 24 (NI)).

This statute is sometimes known informally as the Fifth Reform Act or the Equal Suffrage Act.

Passing of the act

The act was passed by the Conservative Party without much opposition from other parties. This was unsurprising given that the Liberal Party had been behind most reform in the nineteenth century.

RPA 12

The RPA 12 is a fireboat built in 2002 and owned by the Port of Rotterdam.

Numbers

External links

School (TV series)

School!! (スクール!!, Sukūru!!) is a Japanese television series which premiered on Fuji TV on January 16, 2011. It was aired on Fuji TV's Sunday 9:00pm slot "Dramatic Sunday" in the 2011 winter drama season.

Plot

The story is about Shingū Elementary School which is beset by a lot of problems. Seichiro Naruse, a construction worker whose company closed down, suddenly becomes the principal of his old school. He must save the school from closing down.

Cast

Main Cast

The construction company he was working in went bankrupt and he was appointed as the civilian principal of the Shingū Elementary School. Having devoted 20 years in the construction industry, he has no teaching license. He takes up the job in order to keep a promise he made with his former teacher and ex-principal Takeichi. Nonetheless, he has a cheerful personality and takes positive action at every turn, and he frequently describes himself as a "X X demon". He was greatly appalled by the appearance and the change in his alma mater, which was struggling to change the status quo. In the last episode, Shingū Elementary School narrowly avoided closing down, but at the same time, he resigned to take responsibility for the incident that was caused by Akira Hara in the school.

Bleach (Nirvana album)

Bleach is the debut studio album by American rock band Nirvana, released on June 15, 1989 by Sub Pop. The main recording sessions took place at Reciprocal Recording in Seattle, Washington between December 1988 and January 1989.

Bleach was well received by critics, but failed to chart in the U.S. upon its original release. The album was re-released internationally by Geffen Records in 1992 following the success of Nirvana's second album, Nevermind (1991). The re-release debuted at number 89 on the Billboard 200, and peaked at number 33 on the UK Albums Chart and 34 on the Australian albums chart. In 2009 Sub Pop released a 20th anniversary edition of Bleach featuring a live recording of a Nirvana show in Portland, Oregon from 1990 as extra material. Since its release in 1989, Bleach has sold over 1.7 million units in the United States alone. It is Sub Pop's best-selling release to date.

Recording

Following the release of its debut single "Love Buzz" on Sub Pop in November 1988, Nirvana practiced for two to three weeks in preparation for recording a full-length album, even though Sub Pop had only requested an EP. The main sessions for Bleach took place at Reciprocal Recording Studios in Seattle, with local producer Jack Endino.

Crime of the Century (album)

Crime of the Century is the third album by the English progressive rock band Supertramp, released in September 1974. Crime of the Century was Supertramp's commercial breakthrough in both the US and UK, aided by the UK hit "Dreamer" and the U.S. hit "Bloody Well Right". It was a UK Top 10 album and a U.S. Top 40 album, eventually being certified Gold in the U.S. in 1977 after the release of Even in the Quietest Moments.... The album was Supertramp's first to feature drummer Bob Siebenberg (at the time credited as Bob C. Benberg), woodwinds player John Anthony Helliwell, bassist Dougie Thomson, and co-producer Ken Scott.

The album's dedication reads "To Sam", which is a nickname for Stanley August Miesegaes, the Dutch millionaire who supported the band financially from 1969–72.

Background and recording

After the failure of their first two albums and an unsuccessful tour, the band broke up, and Rick Davies and Roger Hodgson recruited new members, drummer Bob C. Benberg, woodwinds player John Helliwell, and bassist Dougie Thomson. This new line-up were sent by their record label, A&M, in particular A&R man Dave Margereson (who would become their manager for the next ten years) to a seventeenth-century farm in Somerset in order to rehearse together and prepare the album.