New York State Route 65

New York State Route 65 (NY 65) is a north–south state highway located in the western portion of New York in the United States. It extends for 18.51 miles (29.79 km) from an intersection with U.S. Route 20 (US 20) and NY 5 in the Ontario County town of West Bloomfield to a junction with NY 96 in the Monroe County town of Brighton. In between, the route serves the village of Honeoye Falls and passes through the extreme northeastern corner of Livingston County. NY 65 intersects NY 251 in Mendon, NY 252 in Pittsford, and the regionally important NY 31 in Brighton. The southern half of NY 65 passes through mostly rural areas, while its northern section traverses densely populated portions of Monroe County.

NY 65 originally began at what is now US 20A in Honeoye when it was assigned as part of the 1930 renumbering of state highways in New York; however, it was truncated to its current southern terminus in the late 1930s. No changes have been made to NY 65's alignment since that time; however, ownership and maintenance of the 1.95-mile (3.14 km) portion of the route north of NY 31 was transferred to Monroe County at some point prior to 1990. This section of NY 65 is co-designated as the unsigned County Route 271 (CR 271).