Primer is a 2004 American indie science fiction drama film about the accidental discovery of a means of time travel. The film was written, directed, produced, edited and scored by Shane Carruth, who also stars in the main role.
Primer is of note for its extremely low budget (completed for $7,000), experimental plot structure, philosophical implications, and complex technical dialogue, which Carruth, a college graduate with a degree in mathematics and a former engineer, chose not to simplify for the sake of the audience. The film collected the Grand Jury Prize at the 2004 Sundance Film Festival, before securing a limited release in the United States, and has since gained a cult following.
Two engineers -- Aaron and Abe -- supplement their day-jobs with entrepreneurial tech projects, working out of Aaron's garage. During one such research effort, involving electromagnetic reduction of objects' weight, the two men accidentally discover an 'A-to-B' time loop side-effect; objects left in the weight-reducing field exhibit temporal anomalies, proceeding normally (from time 'A,' when the field was activated, to time 'B,' when the field is powered off), then backwards (from 'B' back to 'A'), in continuous A-then-B-then-A-then-B sequence, such that objects can leave the field in the present, or at some previous point.
In firearm ballistics, the primer (/ˈpraɪmər/) is a component of pistol, rifle, and shotgun rounds. Upon being struck with sufficient force, a primer reacts chemically to produce heat which ignites the main propellant charge and fires the projectile.
The first step to firing a firearm of any sort is igniting the propellant. The earliest firearms were cannons, which were simple closed tubes. There was a small aperture, the "touchhole", drilled in the closed end of the tube, leading to the main powder charge. This hole was filled with finely ground powder, which was then ignited with a hot ember or torch. With the advent of hand-held firearms, this became an undesirable way of firing a gun. Holding a burning stick while trying to pour a charge of black powder carefully down a barrel is dangerous, and trying to hold the gun with one hand while simultaneously aiming at the target and looking for the touchhole makes it very difficult to fire accurately.
A primer is a strand of short nucleic acid sequences (generally about 10 base pairs) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.
In most cases of natural DNA replication, the primer for DNA synthesis and replication is a short strand of RNA (which can be made de novo).
Many of the laboratory techniques of biochemistry and molecular biology that involve DNA polymerase, such as DNA sequencing and the polymerase chain reaction (PCR), require DNA primers. These primers are usually short, chemically synthesized oligonucleotides, with a length of about twenty bases. They are hybridized to a target DNA, which is then copied by the polymerase.
The lagging strand of DNA is that strand of the DNA double helix that is orientated in a 5' to 3' manner. Therefore, its complement must be synthesized in a 3'→5' manner. Because DNA polymerase III cannot synthesize in the 3'→5' direction, the lagging strand is synthesized in short segments known as Okazaki fragments. Along the lagging strand's template, primase builds RNA primers in short bursts. DNA polymerases are then able to use the free 3'-OH groups on the RNA primers to synthesize DNA in the 5'→3' direction.
&, or ampersand, is a typographic symbol.
& may also refer to:
In the sport of cricket, a single is scored when the batsman take one run, either following a successful shot (with the run attributed to the on-strike batsman) or when running for a bye or leg bye (counted as an extra).
Unlike when a boundary is hit (and the run are scored even if the batsmen don't leave their creases), scoring a single requires the batsmen to physically run between the wickets. This introduces the risk of being run out, so effective communication between the batsmen is vital. If one batsman attempts to run and the other stays put, then a humiliating run out is likely, but quick and well attuned batsmen may be able to run "quick singles" when other batsmen wouldn't. In general, singles are much easier to score when the field is set further out, but bringing more fielders in makes it easier for the on-strike batsman to hit boundaries.
Singles usually rotate the strike in a partnership, but because the bowling end changes at the end of an over, singles deliberately only taken at the end of an over are used by quality batsmen to keep the strike when they are batting with the tail-enders, who are unlikely to survive for long against quality bowling and whom an experienced batsman will normally try to protect.
In Canadian football, a single (single point, or rouge), scoring one point, is awarded when the ball is kicked into the end zone by any legal means, other than a successful field goal, and the receiving team does not return, or kick, the ball out of its end zone. It is also a single if the kick travels through the end zone or goes out of bounds in the end zone without being touched, except on a kickoff. After conceding a single, the receiving team is awarded possession of the ball at the 35-yard line of its own end of the field.
Singles are not awarded in the following situations:
In all these cases the defending team is awarded possession of the ball at the 25-yard line.
In the United States, singles are not usually recognized in most leagues and are awarded only in matches played under the auspices of the National Indoor Football League (formerly United Indoor Football) and the now-defunct American Indoor Football Association. It is applied only on kickoffs in both leagues, and is scored if the receiving team fails to advance the ball out of the end zone when kicked. The NIFL also allowed a single to be scored by kicking a kickoff through the uprights (as in a field goal); this type of single is nicknamed (and has since been codified in the NIFL rules as) an uno, from the Spanish word for the number one. At one point, the Philadelphia Public League (the public high school football sanctioning body in Philadelphia, Pennsylvania) awarded three points for kicking a kickoff through the uprights.
Viaje is a 2015 Costa Rican romance film directed and written by Paz Fábrega. It had its world premiere at the Tribeca Film Festival 2015, where it met with possitive reviews . The film is shot in black-and-white.